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  • 1.
    Figueiredo, Carina
    et al.
    CSIC, Inst Catalisis & Petroleoquim, C-Marie Curie 2, Madrid 28049, Spain..
    Psotta, Carolin
    Malmö universitet, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV). Malmö universitet, Biofilms Research Center for Biointerfaces. Aptusens AB, S-29394 Kyrkhult, Sweden..
    Jayakumar, Kavita
    Univ Galway, Sch Biol & Chem Sci, Galway H91 TK33, Ireland.;Univ Galway, Ryan Inst, Galway H91 TK33, Ireland..
    Lielpetere, Anna
    Ruhr Univ Bochum, Fac Chem & Biochem, Analyt Chem Ctr Electrochem Sci CES, D-44791 Bochum, Germany..
    Mandal, Tanushree
    Univ Galway, Sch Biol & Chem Sci, Galway H91 TK33, Ireland.;Univ Galway, Ryan Inst, Galway H91 TK33, Ireland..
    Schuhmann, Wolfgang
    Ruhr Univ Bochum, Fac Chem & Biochem, Analyt Chem Ctr Electrochem Sci CES, D-44791 Bochum, Germany..
    Leech, Donal
    Univ Galway, Sch Biol & Chem Sci, Galway H91 TK33, Ireland.;Univ Galway, Ryan Inst, Galway H91 TK33, Ireland..
    Falk, Magnus
    Malmö universitet, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV). Malmö universitet, Biofilms Research Center for Biointerfaces.
    Pita, Marcos
    CSIC, Inst Catalisis & Petroleoquim, C-Marie Curie 2, Madrid 28049, Spain..
    Shleev, Sergey
    Malmö universitet, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV). Malmö universitet, Biofilms Research Center for Biointerfaces. Aptusens AB, S-29394 Kyrkhult, Sweden..
    De Lacey, Antonio L.
    CSIC, Inst Catalisis & Petroleoquim, C-Marie Curie 2, Madrid 28049, Spain..
    Effect of Protection Polymer Coatings on the Performance of an Amperometric Galactose Biosensor in Human Plasma2024Ingår i: Biosensors, ISSN 2079-6374, Vol. 14, nr 4, artikel-id 167Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Galactose monitoring in individuals allows the prevention of harsh health conditions related to hereditary metabolic diseases like galactosemia. Current methods of galactose detection need development to obtain cheaper, more reliable, and more specific sensors. Enzyme-containing amperometric sensors based on galactose oxidase activity are a promising approach, which can be enhanced by means of their inclusion in a redox polymer coating. This strategy simultaneously allows the immobilization of the biocatalyst to the electroactive surface and hosts the electron shuttling units. An additional deposition of capping polymers prevents external interferences like ascorbic or uric acid as well as biofouling when measuring in physiological fuels. This work studies the protection effect of poly(2-methacryloyloxyethyl phosphorylcholine-co-glycidyl methacrylate (MPC) and polyvinylimidazole-polysulfostyrene (P(VI-SS)) when incorporated in the biosensor design for the detection of galactose in human plasma.

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  • 2.
    Psotta, Carolin
    et al.
    Malmö universitet, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV). Malmö universitet, Biofilms Research Center for Biointerfaces. Aptusens AB, S-29394 Kyrkhult, Sweden..
    Cirovic, Stefan
    Malmö universitet, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV). Malmö universitet, Biofilms Research Center for Biointerfaces.
    Gudmundsson, Petri
    Malmö universitet, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV). Malmö universitet, Biofilms Research Center for Biointerfaces.
    Falk, Magnus
    Malmö universitet, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV). Malmö universitet, Biofilms Research Center for Biointerfaces.
    Mandal, Tanushree
    Univ Galway, Sch Chem & Ryan Inst, Univ Rd, Galway, Ireland..
    Reichhart, Thomas
    Univ Nat Resources & Life Sci, Dept Food Sci & Technol, BOKU, A-1190 Vienna, Austria.;DirectSens Biosensors GmbH, A-3400 Klosterneuburg, Austria..
    Leech, Donal
    Univ Galway, Sch Chem & Ryan Inst, Univ Rd, Galway, Ireland..
    Ludwig, Roland
    Univ Nat Resources & Life Sci, Dept Food Sci & Technol, BOKU, A-1190 Vienna, Austria.;DirectSens Biosensors GmbH, A-3400 Klosterneuburg, Austria..
    Kittel, Roman
    Univ Nat Resources & Life Sci, Dept Food Sci & Technol, BOKU, A-1190 Vienna, Austria..
    Schuhmann, Wolfgang
    Ruhr Univ Bochum, Fac Chem & Biochem, Analyt Chem Ctr Electrochem Sci, D-44780 Bochum, Germany..
    Shleev, Sergey
    Malmö universitet, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV). Malmö universitet, Biofilms Research Center for Biointerfaces. Aptusens AB, S-29394 Kyrkhult, Sweden..
    Continuous ex vivo glucose sensing in human physiological fluids using an enzymatic sensor in a vein replica2023Ingår i: Bioelectrochemistry, ISSN 1567-5394, E-ISSN 1878-562X, Vol. 152, artikel-id 108441Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Managing blood glucose can affect important clinical outcomes during the intraoperative phase of surgery. However, currently available instruments for glucose monitoring during surgery are few and not optimized for the specific application. Here we report an attempt to exploit an enzymatic sensor in a vein replica that could continuously monitor glucose level in an authentic human bloodstream. First, detailed investigations of the superficial venous systems of volunteers were carried out using ocular and palpating examinations, as well as advanced ultrasound measurements. Second, a tubular glucose-sensitive biosensor mimicking a venous system was designed and tested. Almost ideal linear dependence of current output on glucose concentration in phosphate buffer saline was obtained in the range 2.2-22.0 mM, whereas the dependence in human plasma was less linear. Finally, the developed biosensor was investigated in whole blood under homeostatic conditions. A specific correlation was found between the current output and glucose concentration at the initial stage of the biodevice operation. However, with time, blood coagulation during measurements negatively affected the performance of the biodevice. When the experimental results were remodeled to predict the response without the influence of blood coagulation, the sensor output closely followed the blood glucose level.

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  • 3.
    Falk, Magnus
    et al.
    Malmö universitet, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV). Malmö universitet, Biofilms Research Center for Biointerfaces.
    Psotta, Carolin
    Malmö universitet, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV). Malmö universitet, Biofilms Research Center for Biointerfaces.
    Cirovic, Stefan
    Malmö universitet, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV). Malmö universitet, Biofilms Research Center for Biointerfaces.
    Ohlsson, Lars
    Malmö universitet, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV). Malmö universitet, Biofilms Research Center for Biointerfaces.
    Shleev, Sergey
    Malmö universitet, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV). Malmö universitet, Biofilms Research Center for Biointerfaces.
    Electronic Tongue for Direct Assessment of SARS-CoV-2-Free and Infected Human Saliva-A Feasibility Study2023Ingår i: Biosensors, ISSN 2079-6374, Vol. 13, nr 7, artikel-id 717Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    An electronic tongue is a powerful analytical instrument based on an array of non-selective chemical sensors with a partial specificity for data gathering and advanced pattern recognition methods for data analysis. Connecting electronic tongues with electrochemical techniques for data collection has led to various applications, mostly within sensing for food quality and environmental monitoring, but also in biomedical research for the analyses of different bioanalytes in human physiological fluids. In this paper, an electronic tongue consisting of six electrodes (viz., gold, platinum, palladium, titanium, iridium, and glassy carbon) was designed and tested in authentic (undiluted, unpretreated) human saliva samples from eight volunteers, collected before and during the COVID-19 pandemic. Investigations of 11 samples using differential pulse voltammetry and a principal component analysis allowed us to distinguish between SARS-CoV-2-free and infected authentic human saliva. This work, as a proof-of-principle demonstration, provides a new perspective for the use of electronic tongues in the field of enzyme-free electrochemical biosensing, highlighting their potential for future applications in non-invasive biomedical analyses.

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  • 4.
    Ramji, Rathi
    et al.
    Malmö universitet, Fakulteten för hälsa och samhälle (HS), Institutionen för vårdvetenskap (VV).
    Rämgård, Margareta
    Malmö universitet, Fakulteten för hälsa och samhälle (HS), Institutionen för vårdvetenskap (VV).
    Carlson, Elisabeth
    Malmö universitet, Fakulteten för hälsa och samhälle (HS), Institutionen för vårdvetenskap (VV).
    Shleev, Sergey
    Malmö universitet, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV). Malmö universitet, Biofilms Research Center for Biointerfaces.
    Awad, Eman
    Malmö universitet, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV).
    Cirovic, Stefan
    Malmö universitet, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV). Malmö universitet, Biofilms Research Center for Biointerfaces.
    Kottorp, Anders
    Malmö universitet, Fakulteten för hälsa och samhälle (HS), Institutionen för vårdvetenskap (VV).
    Health and quality of life among women after participation in a CBPR-informed physical activity intervention: with a pandemic perspective.2023Ingår i: Scientific Reports, E-ISSN 2045-2322, Vol. 13, nr 1, artikel-id 17972Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    The lack of culturally and contextually oriented interventions promoting physical activity (PA) has led to increased physical inactivity among women living in disadvantaged neighbourhoods in Sweden. In this study one such intervention informed by community-based participatory research (CBPR) has been evaluated among 34 women from a disadvantaged neighbourhood before and during COVID-19. Health-related quality of life (HRQOL), behavioural and biomedical outcomes were assessed directly prior and post-intervention, followed by evaluations at 6-months and 18-months follow-up during COVID-19. The results revealed that HRQOL, particularly psychological, social, and environmental health significantly increased post-intervention compared to prior to intervention but reversed back at 6-months follow-up. Perceived health satisfaction and environmental health increased at 18-months follow-up during COVID-19. Participation in PA improved post-intervention and at 6-months follow-up. Everyday activities and fruit and vegetable intake continued to increase through all timepoints. Systolic blood pressure significantly decreased post-intervention and 6-months follow-up; blood flow rate increased significantly at all timepoints. Overall, the findings underscores the potential effectiveness of CBPR approaches in promoting and sustaining healthy lifestyles, even during acute situations such as the COVID-19. It may even serve as a future model for promoting health and addressing health disparities in similar groups.

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  • 5.
    Aleksejeva, Olga
    et al.
    Malmö universitet, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV).
    Nilsson, N.
    Obducat Technol AB, S-22363 Lund, Sweden..
    Genevskiy, Vladislav
    Malmö universitet, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV).
    Thulin, K.
    Obducat Technol AB, S-22363 Lund, Sweden..
    Shleev, Sergey
    Malmö universitet, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV).
    Dual-feature photobioanodes based on nanoimprint lithography for photoelectric biosupercapacitors2022Ingår i: Journal of Power Sources, ISSN 0378-7753, E-ISSN 1873-2755, Vol. 517, artikel-id 230677Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Direct transformation of solar energy into electrical energy by means of biological photosynthesis is considered as an attractive option for sustainable electrical energy production. Thylakoid membranes, the site of photosynthesis, are regarded as a promising biological material for the development of photoelectric biodevices, which produce electrical power consuming only light energy as oxygen evolves at photobioanode upon irradiation and biocathode converts it back to water. Therefore, in this work dual-feature photobioanode based on nanoimprinted gold substrates modified with thylakoids in combination with a capacitive part made of a planar gold substrate coated with a conductive polymer was designed and evaluated, providing open-circuit potential of -0.21 V vs. Ag vertical bar AgCl vertical bar KClsat and a capacitance of ca. 60 F m(-2) both at ambient light and artificial illumination of 400 W m(-2). Combination of thylakoid based dual-feature photobioanode with bilirubin oxidase modified transparent and capacitive indium tin oxide biocathode resulted in a photoelectric biosupercapacitor with remarkable characteristics at ambient light, viz. an open-circuit voltage as high as 0.74 V, which was stable upon charge-discharge cycles during ca. 2 h.

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  • 6.
    Ramonas, Eimantas
    et al.
    Vilnius Univ, Life Sci Ctr, Inst Biochem, Dept Bioanal, Sauletekio Al 7, LT-10257 Vilnius, Lithuania.;Vilnius Gediminas Tech Univ, Dept Chem & Bioengn, Sauletekio Al 11, LT-10223 Vilnius, Lithuania..
    Butkevicius, Marius
    Vilnius Univ, Life Sci Ctr, Inst Biochem, Dept Bioanal, Sauletekio Al 7, LT-10257 Vilnius, Lithuania..
    Shleev, Sergey
    Malmö universitet, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV). Malmö universitet, Biofilms Research Center for Biointerfaces.
    Dagys, Marius
    Vilnius Univ, Life Sci Ctr, Inst Biochem, Dept Bioanal, Sauletekio Al 7, LT-10257 Vilnius, Lithuania..
    Ratautas, Dalius
    Vilnius Univ, Life Sci Ctr, Inst Biochem, Dept Bioanal, Sauletekio Al 7, LT-10257 Vilnius, Lithuania.;Vilnius Gediminas Tech Univ, Dept Chem & Bioengn, Sauletekio Al 11, LT-10223 Vilnius, Lithuania..
    Mechanistic characterization of an oxygen reduction reaction-driven, fully enzymatic and self-calibrating pH biosensor based on wired bilirubin oxidase2022Ingår i: Sensors and actuators. B, Chemical, ISSN 0925-4005, E-ISSN 1873-3077, Vol. 367, artikel-id 132054Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    The oxygen reduction reaction (ORR) is important due to high electrochemical potential. Multicopper oxidases (e.g., bilirubin oxidase (BOx)) belong to a class of enzymes that catalyze ORR with low overpotential, and have attracted significant attention in designing bioelectronic devices, viz., biosensors and enzymatic cathodes of biofuel cells. In this work, we present a new concept - an enzyme driven pH electrode system composed of two electrodes: a pH-sensitive BOx-based working electrode and pH-insensitive, a BOx-based reference electrode. ORR-driven pH monitoring was conducted by measuring the potential difference between the two electrodes. Moreover, the electrode system was self-supported and self-calibrating, and no additional electrodes (Pt counter or Ag/AgCl reference) were needed for measurements. The BOx-based pH sensor was tested in continuous and real-time pH monitoring with high accuracy. Also, we present an in-depth mechanistic study explaining the electrode potential-pH dependence, which brings new insights into the operational principle of bilirubin oxidase at different pH.

  • 7.
    Aleksejeva, Olga
    et al.
    Malmö universitet, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV).
    Nilsson, Nicklas
    Obducat Technol AB, S-22363 Lund, Sweden..
    Genevskiy, Vladislav
    Malmö universitet, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV).
    Thulin, Kristian
    Obducat Technol AB, S-22363 Lund, Sweden..
    Shleev, Sergey
    Malmö universitet, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV).
    Photobioanodes Based on Nanoimprinted Electrodes and Immobilized Chloroplasts2022Ingår i: ChemElectroChem, E-ISSN 2196-0216, Vol. 9, nr 2, s. 37-42Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    As the global energy demand continues to increase, the interest in photosynthetic energy conversion is growing accordingly. Chloroplasts, photosynthetic organelles present in plants and algae, are attractive candidates for construction of bio solar cells; however, they have been less studied because of their complex membrane system, which restricts electrochemical communication with an electrode surface. Nevertheless, in this work photobioanodes based on planar and nanoimprinted gold substrates modified with chloroplasts were designed and evaluated. Apparently, nanoimprint lithography contributed to higher photocurrent densities, not only owing to the enlarged real surface area, but also due to boosting electrochemical communication between the photosynthetic organelles and the electrode. Combining chloroplast-modified nanoimprinted gold electrodes with a capacitive part made of a planar gold substrate, coated with a conductive polymer, resulted in a dual-feature photobioanode providing a lower open-circuit potential, i. e., -0.11 V vs. Ag|AgCl|KClsat, and an enhanced capacitance of ca. 37 F m(-2) upon illumination of 400 W m(-2).

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  • 8.
    Aleksejeva, Olga
    et al.
    Malmö universitet, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV).
    Sokolov, A. V.
    Russia Saint-Petersburg State University, Russia.
    Marquez, I.
    University of Seville, Spain.
    Gustafsson, Anna
    Malmö universitet, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV).
    Bushnev, S.
    Russian Academy of Sciences, Russia.
    Eriksson, Håkan
    Malmö universitet, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV).
    Ljunggren, Lennart
    Malmö universitet, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV).
    Shleev, Sergey
    Malmö universitet, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV). Russian Academy of Sciences, Russia.
    Autotolerant ceruloplasmin based biocathodes for implanted biological power sources2021Ingår i: Bioelectrochemistry, ISSN 1567-5394, E-ISSN 1878-562X, Vol. 140Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    High-performance autotolerant bioelectrodes should be ideally suited to design implantable bioelectronic devices. Because of its high redox potential and ability to reduce oxygen directly to water, human ceruloplasmin, HCp, the only blue multicopper oxidase present in human plasma, appears to be the ultimate biocatalyst for oxygen biosensors and also biocathodes in biological power sources. In comparison to fungal and plant blue multicopper oxidases, e.g. Myrothecium verrucaria bilirubin oxidase and Rhus vernicifera laccase, respectively, the inflammatory response to HCp in human blood is significantly reduced. Partial purification of HCp allowed to preserve the native conformation of the enzyme and its biocatalytic activity. Therefore, electrochemical studies were carried out with the partially purified enzyme immobilised on nanostructured graphite electrodes at physiological pH and temperature. Amperometric investigations revealed low reductive current densities, i.e. about 1.65 µA cm−2 in oxygenated electrolyte and in the absence of any mediator, demonstrating nevertheless direct electron transfer based O2 bioelectroreduction by HCp for the first time. The reductive current density obtained in the mediated system was about 12 µA cm−2. Even though the inflammatory response of HCp is diminished in human blood, inadequate bioelectrocatalytic performance hinders its use as a cathodic bioelement in a biofuel cell.

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  • 9.
    Awad, Eman
    et al.
    Malmö universitet, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV).
    Ramji, Rathi
    Malmö universitet, Fakulteten för hälsa och samhälle (HS), Institutionen för vårdvetenskap (VV).
    Cirovic, Stefan
    Malmö universitet, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV).
    Rämgård, Margareta
    Malmö universitet, Fakulteten för hälsa och samhälle (HS), Institutionen för vårdvetenskap (VV).
    Kottorp, Anders
    Malmö universitet, Fakulteten för hälsa och samhälle (HS), Institutionen för vårdvetenskap (VV).
    Shleev, Sergey
    Malmö universitet, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV). Malmö University.
    Developing and evaluating non-invasive healthcare technologies for a group of female participants from a socioeconomically disadvantaged area2021Ingår i: Scientific Reports, E-ISSN 2045-2322, Vol. 11, nr 1, artikel-id 23896Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    When compared to the general population, socioeconomically disadvantaged communitiesfrequently experience compromised health. Monitoring the divide is challenging since standardizedbiomedical tests are linguistically and culturally inappropriate. The aim of this study was to developand test a unique mobile biomedical testbed based on non-invasive analysis, as well as to explorethe relationships between the objective health measures and subjective health outcomes, asevaluated with the World Health Organization Quality of Life survey. The testbed was evaluated in asocioeconomically disadvantaged neighborhood in Malmö, which has been listed as one of the twelvemost vulnerable districts in Sweden. The study revealed that compared to conventional protocolsthe less intrusive biomedical approach was highly appreciated by the participants. Surprisingly, thecollected biomedical data illustrated that the apparent health of the participants from the ethnicallydiverse low-income neighborhood was comparable to the general Swedish population. Statisticallysignificant correlations between perceived health and biomedical data were disclosed, even thoughthe dependences found were complex, and recognition of the manifest complexity needs to beincluded in further research. Our results validate the potential of non-invasive technologies incombination with advanced statistical analysis, especially when combined with linguistically andculturally appropriate healthcare methodologies, allowing participants to appreciate the significanceof the different parameters to evaluate and monitor aspects of health.

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  • 10.
    Andoralov, Victor
    et al.
    Malmö universitet, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV).
    Shleev, Sergey
    Malmö universitet, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV). Malmö universitet, Biofilms Research Center for Biointerfaces. Research Centre of Biotechnology, Russian Academy of Sciences, 119071 Moscow, Russia.
    Dergousova, Natalia
    Research Centre of Biotechnology, Russian Academy of Sciences, 119071 Moscow, Russia.
    Kulikova, Olga
    Research Centre of Biotechnology, Russian Academy of Sciences, 119071 Moscow, Russia.
    Popov, Vladimir
    Research Centre of Biotechnology, Russian Academy of Sciences, 119071 Moscow, Russia; Kurchatov NBIC Centre, National Research Centre “Kurchatov Institute”, 123182 Moscow, Russia.
    Tikhonova, Tamara
    Research Centre of Biotechnology, Russian Academy of Sciences, 119071 Moscow, Russia.
    Octaheme nitrite reductase: The mechanism of intramolecular electron transfer and kinetics of nitrite bioelectroreduction.2021Ingår i: Bioelectrochemistry, ISSN 1567-5394, E-ISSN 1878-562X, Vol. 138, artikel-id 107699Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Detailed impedance and voltammetric studies of hexameric octaheme nitrite reductase immobilized on carbon-based nanomaterials, specifically nanotubes and nanoparticles, were performed. Well-pronounced bioelectrocatalytic reduction of nitrite on enzyme-modified electrodes was obtained. Analysis of the impedance data indicated the absence of long-lived intermediates involved in the nitrite reduction. Cyclic voltammograms of biomodified electrodes had a bi-sigmoidal shape, which pointed to the presence of two enzyme orientations on carbon supports. The maximum (limiting) catalytic currents were determined and, by applying the correction by the mixed kinetics equation, the Tafel dependences were plotted for each catalytic wave/each enzyme orientation. Finally, two schemes for the rate-limiting processes during bioelectrocatalysis were proposed, viz. for low- and high-potential orientations.

  • 11.
    Falk, Magnus
    et al.
    Malmö universitet, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV). Malmö universitet, Biofilms Research Center for Biointerfaces.
    Nilsson, Emelie J.
    Malmö universitet, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV). Malmö universitet, Biofilms Research Center for Biointerfaces.
    Cirovic, Stefan
    Malmö universitet, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV). Malmö universitet, Biofilms Research Center for Biointerfaces.
    Tudosoiu, Bogdan
    Covercast AB, Drottensgatan 4, 222 23 Lund, Sweden.
    Shleev, Sergey
    Malmö universitet, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV). Malmö universitet, Biofilms Research Center for Biointerfaces.
    Wearable Electronic Tongue for Non-Invasive Assessment of Human Sweat2021Ingår i: Sensors, E-ISSN 1424-8220, Vol. 21, nr 21, artikel-id 7311Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Sweat is a promising biofluid in allowing for non-invasive sampling. Here, we investigate the use of a voltammetric electronic tongue, combining different metal electrodes, for the purpose of non-invasive sample assessment, specifically focusing on sweat. A wearable electronic tongue is presented by incorporating metal electrodes on a flexible circuit board and used to non-invasively monitor sweat on the body. The data obtained from the measurements were treated by multivariate data processing. Using principal component analysis to analyze the data collected by the wearable electronic tongue enabled differentiation of sweat samples of different chemical composition, and when combined with 1H-NMR sample differentiation could be attributed to changing analyte concentrations.

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  • 12.
    Ramji, Rathi
    et al.
    Malmö universitet, Fakulteten för hälsa och samhälle (HS), Institutionen för vårdvetenskap (VV).
    Carlson, Elisabeth
    Malmö universitet, Fakulteten för hälsa och samhälle (HS), Institutionen för vårdvetenskap (VV).
    Kottorp, Anders
    Malmö universitet, Fakulteten för hälsa och samhälle (HS), Institutionen för vårdvetenskap (VV).
    Shleev, Sergey
    Malmö universitet, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV). Malmö universitet, Biofilms Research Center for Biointerfaces.
    Awad, Eman
    Malmö universitet, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV).
    Rämgård, Margareta
    Malmö universitet, Fakulteten för hälsa och samhälle (HS), Institutionen för vårdvetenskap (VV).
    Development and evaluation of a physical activity intervention informed by participatory research: a feasibility study2020Ingår i: BMC Public Health, E-ISSN 1471-2458, Vol. 20, artikel-id 112Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Background: Despite numerous interventions aiming to improve physical activity in socially disadvantagedpopulations, physical inactivity remains to be a rising challenge to public health globally, as well as, in Sweden. Inan effort to address this challenge, a community-based participatory intervention was developed through activecommunity engagement and implemented in a socially disadvantaged neighborhood in Sweden. The current studyaims to present the development and initial evaluation of a participatory research driven physical activityintervention.Methods: Fifteen participants (11 females and 4 males) aged 17–59 years volunteered to participate in the physicalactivity intervention program. The intervention program was evaluated using a longitudinal mixed methods designmeasuring health impact changes over time through focus group discussions and quality of life surveys. Furtheradditional biomedical health parameters such as levels of glycosylated hemoglobin, blood pressure, levels ofoxygen saturation and body mass index were monitored before and after the intervention. Focus group data wereanalyzed using content analysis with an inductive approach. The pre-and post-test scores from the survey-basedquality of life domains, as well as the health parameters were compared using non-parametric and parametricstatistics.Results: Four themes emerged from the analysis of the focus group discussions including sense of fellowship,striving for inclusion and equity, changing the learner perspective and health beyond illness. The scores for thedomains Physical Health, Psychological Health, Social Relationships and Health Satisfaction where significantlyhigher after participation in the physical activity intervention program compared to the pre-test scores (p < .05)s.There were however, no significant changes in the scores for the environmental domain and overall quality of lifeafter intervention compared to that prior to intervention start. Overall, the biomedical health parameters remainedstable within the normal ranges during intervention.Conclusion: The focus group discussions and results from the surveys and biomedical measures reveal importantfindings to understand and further develop the intervention program to promote health equity among citizens indisadvantaged areas. Evaluating the feasibility of such an intervention using multiple approaches contributes toeffective implementation of it for larger communities in need.

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  • 13.
    Falk, Magnus
    et al.
    Malmö universitet, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV). Malmö universitet, Biofilms Research Center for Biointerfaces.
    Psotta, Carolin
    Malmö universitet, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV). Malmö universitet, Biofilms Research Center for Biointerfaces. Aptusens AB.
    Cirovic, Stefan
    Malmö universitet, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV). Malmö universitet, Biofilms Research Center for Biointerfaces.
    Shleev, Sergey
    Malmö universitet, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV). Malmö universitet, Biofilms Research Center for Biointerfaces. Aptusens AB.
    Non-Invasive Electrochemical Biosensors Operating in Human Physiological Fluids2020Ingår i: Sensors, E-ISSN 1424-8220, Vol. 20, nr 21, s. 1-28, artikel-id 6352Artikel, forskningsöversikt (Refereegranskat)
    Abstract [en]

    Non-invasive healthcare technologies are an important part of research and development nowadays due to the low cost and convenience offered to both healthcare receivers and providers. This work overviews the recent advances in the field of non-invasive electrochemical biosensors operating in secreted human physiological fluids, viz. tears, sweat, saliva, and urine. Described electrochemical devices are based on different electrochemical techniques, viz. amperometry, coulometry, cyclic voltammetry, and impedance spectroscopy. Challenges that confront researchers in this exciting area and key requirements for biodevices are discussed. It is concluded that the field of non-invasive sensing of biomarkers in bodily fluid is highly convoluted. Nonetheless, if the drawbacks are appropriately addressed, and the pitfalls are adroitly circumvented, the approach will most certainly disrupt current clinical and self-monitoring practices.

  • 14.
    Shleev, Sergey
    et al.
    Malmö universitet, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV).
    Aleksejeva, Olga
    Malmö universitet, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV).
    Falk, Magnus
    Malmö universitet, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV).
    Blum, Zoltan
    Malmö universitet, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV).
    Biodegradable electric power devices2019Ingår i: Bioelectrochemistry: Design and Applications of Biomaterials / [ed] Serge Cosnier, Walter de Gruyter, 2019, s. 237-258Kapitel i bok, del av antologi (Refereegranskat)
  • 15. Laurynenas, Audrius
    et al.
    Butkevicius, Marius
    Dagys, Marius
    Shleev, Sergey
    Malmö universitet, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV).
    Kulys, Juozas
    Consecutive Marcus Electron and Proton Transfer in Heme Peroxidase Compound II-Catalysed Oxidation Revealed by Arrhenius Plots2019Ingår i: Scientific Reports, E-ISSN 2045-2322, Vol. 9, artikel-id 14092Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Electron and proton transfer reactions in enzymes are enigmatic and have attracted a great deal of theoretical, experimental, and practical attention. The oxidoreductases provide model systems for testing theoretical predictions, applying experimental techniques to gain insight into catalytic mechanisms, and creating industrially important bio(electro) conversion processes. Most previous and ongoing research on enzymatic electron transfer has exploited a theoretically and practically sound but limited approach that uses a series of structurally similar ("homologous") substrates, measures reaction rate constants and Gibbs free energies of reactions, and analyses trends predicted by electron transfer theory. This approach, proposed half a century ago, is based on a hitherto unproved hypothesis that pre-exponential factors of rate constants are similar for homologous substrates. Here, we propose a novel approach to investigating electron and proton transfer catalysed by oxidoreductases. We demonstrate the validity of this new approach for elucidating the kinetics of oxidation of "non-homologous" substrates catalysed by compound II of Coprinopsis cinerea and Armoracia rusticana peroxidases. This study-using the Marcus theory-demonstrates that reactions are not only limited by electron transfer, but a proton is transferred after the electron transfer event and thus both events control the reaction rate of peroxidase-catalysed oxidation of substrates.

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  • 16.
    Aleksejeva, Olga
    et al.
    Malmö universitet, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV).
    Mateljak, Ivan
    Ludwig, Roland
    Alcalde, Miguel
    Shleev, Sergey
    Malmö universitet, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV).
    Electrochemistry of a high redox potential laccase obtained by computer-guided mutagenesis combined with directed evolution2019Ingår i: Electrochemistry communications, ISSN 1388-2481, E-ISSN 1873-1902, Vol. 106, artikel-id UNSP 106511Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Electrochemical characterization of the GreeDo variant of a high redox potential fungal laccase obtained by laboratory evolution together with computer-guided mutagenesis, in comparison to its parental variety (the OB-1 mutant), is presented. Both laccases, when immobilized on graphite electrodes either by direct physical adsorption or covalently attached via gold nanoparticles, were capable of both non-mediated and mediator-based bioelectroreduction of molecular oxygen at low overpotentials. GreeDo exhibited higher open circuit potential values and onset potentials for oxygen bioelectroreduction compared to OB-1. However, even though in homogeneous catalysis GreeDo outperforms OB-1 in terms of turnover numbers and catalytic efficiency, when exposed to high redox potential substrates, direct electron transfer based bioelectrocatalytic currents of GreeDo and OB-1 modified electrodes were similar. High operational stability of freely diffusing GreeDo and also the immobilized enzyme in the acidic electrolyte was registered, in agreement with high storage stability of GreeDo in acidic solutions.

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  • 17. Mateljak, Ivan
    et al.
    Monza, Emanuele
    Fatima Lucas, Maria
    Guallar, Victor
    Aleksejeva, Olga
    Malmö universitet, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV).
    Ludwig, Roland
    Leech, Donal
    Shleev, Sergey
    Malmö universitet, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV).
    Alcalde, Miguel
    Increasing Redox Potential, Redox Mediator Activity, and Stability in a Fungal Laccase by Computer-Guided Mutagenesis and Directed Evolution2019Ingår i: ACS Catalysis, ISSN 2155-5435, E-ISSN 2155-5435, Vol. 9, nr 5, s. 4561-4572Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Fungal high-redox-potential laccases (HRPLs) are multi-copper oxidases with a relaxed substrate specificity that is highly dependent on their binding affinity and redox potential of the T1Cu site (E-T1). In this study, we combined computational design with directed evolution to tailor an HRPL variant with increased E-T1 and activity toward high-redox-potential mediators as well as enhanced stability. Laccase mutant libraries were screened in vitro using synthetic highredox-potential mediators with different oxidation routes and chemical natures, while computer-aided evolution experiments were run in parallel to guide benchtop mutagenesis, without compromising protein stability. Through this strategy, the E-T1 of the evolved HRPL increased from 740 to 790 mV, with a concomitant improvement in thermal and acidic pH stability. The kinetic constants for high-redox-potential mediators were markedly improved and were then successfully tested within laccase systems (LMSs). Two hydrophobic substitutions surrounding the T1Cu site appeared to underlie these effects, and they were rationalized at the atomic level. Together, this study represents a proof-of-concept of the joint elevation of the E-T1, redox mediator activity, and stability in an HRPL, making this versatile biocatalyst a promising candidate for future LMS applications and for the development of bioelectrochemical devices.

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  • 18. Jarne, Carmen
    et al.
    Paul, Logan
    Carlos Conesa, Jose
    Shleev, Sergey
    Malmö universitet, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV).
    De Lacey, Antonio L.
    Pita, Marcos
    Underpotential Photoelectrooxidation of Water by SnS2-Laccase Co-catalysts on Nanostructured Electrodes with Only Visible-Light Irradiation2019Ingår i: ChemElectroChem, E-ISSN 2196-0216, Vol. 6, nr 10, s. 2755-2761Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    More sustainable ways to produce and store energy are urgently needed to reduce our dependence on fossil fuels, which are the principal drivers of global warming and pollution. Hydrogen may become the energy vector needed for this purpose if its production through water splitting can become competitive against steam methane reforming. Even after decades of research, the proposed strategies for water splitting are not efficient enough to overcome the high overpotential of the water oxidation reaction. In a quest for new approaches to this problem, recent studies have attempted to combine inorganic catalysts with biocatalysts, aiming to open new possibilities towards a definitive solution. In the present work we have tested a chalcogenide semiconductor, SnS2, characterized by a deep valence band and a visible-light band gap of approximately 2.2eV (lambda=550nm). Preparation of a fluorine-doped tin oxide electrode modified with SnS2 and laccase allowed water oxidation at a lower overpotential, taking better advantage of light energy. Additionally, indium tin oxide nanoparticles were added to increase the contact area between SnS2 and the electrode surface and thereby improve charge separation for photobioelectrocatalytic water oxidation. We tested the nanostructured anode electrodes under different applied potentials and irradiance intensities from a solar simulator to find the optimal photonic and faradaic efficiencies.

  • 19.
    Shleev, Sergey
    et al.
    Malmö universitet, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV).
    Falk, Magnus
    Malmö universitet, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV).
    Cirovic, Stefan
    Malmö universitet, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV).
    Blum, Zoltan
    Malmö universitet, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV).
    Wearable bioelectronic devices2019Ingår i: Bioelectrochemistry: Design and Applications of Biomaterials / [ed] Serge Cosnier, Walter de Gruyter, 2019, s. 213-236Kapitel i bok, del av antologi (Refereegranskat)
  • 20.
    Gonzalez-Arribas, Elena
    et al.
    Malmö universitet, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV).
    Falk, Magnus
    Malmö universitet, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV).
    Aleksejeva, Olga
    Malmö universitet, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV).
    Bushnev, Sergey
    Sebastian, Paula
    Feliu, Juan M.
    Shleev, Sergey
    Malmö universitet, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV).
    A conventional symmetric biosupercapacitor based on rusticyanin modified gold electrodes2018Ingår i: Journal of Electroanalytical Chemistry, ISSN 1572-6657, Vol. 816, s. 253-258Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Here we report on an entirely new kind of bioelectronic device - a conventional biosupercapacitor, which is built from copper containing redox proteins. Prior to biodevice fabrication, detailed spectroelectrochemical studies of the protein, viz. Acidithiobacillus ferrooxidcats rusticyanin, in solution and in adsorbed state, were performed, including estimation of the redox potential of the T1 site (0.62 V vs. NHE), protein midpoint potential when adsorbed on a self-assembled monolayer (0.34 V vs. NHE), as well as biocapacitance of rusticyanin modified gold electrodes (115 mu F cm(-2)). The symmetrical biosupercapacitor based on two identical gold electrodes modified with rusticyanin is able to capacitively store electricity and deliver electric power accumulated mostly in the form of biopseudocapacitance, when charged and discharged externally. When charged during Just 5 s, the biosupercapacitor with a total capacitance of about 73 mu F cm(-2) provided a maximum of 4 mu A cm(-2) peak current at 0.40 V. The biodevice, which can be charged and discharged at least 50 times without a significant loss of ability to store electric energy, had a low leakage current below 50 nA cm(-2).

  • 21. Zebda, Abdelkader
    et al.
    Alcaraz, Jean-Pierre
    Vadgama, Pankaj
    Shleev, Sergey
    Malmö universitet, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV).
    Minteer, Shelley D.
    Boucher, Francois
    Cinquin, Philippe
    Martin, Donald K.
    Challenges for successful implantation of biofuel cells2018Ingår i: Bioelectrochemistry, ISSN 1567-5394, E-ISSN 1878-562X, Vol. 124, s. 57-72Artikel, forskningsöversikt (Refereegranskat)
    Abstract [en]

    There is a growing interest in the design and engineering of operational biofuel cells that can be implanted. This review highlights the recent progress in the electrochemistry of biofuel cell technologies, but with a particular emphasis on the medical and physiological aspects that impact the biocompatibility of biofuel cells operating inside a living body. We discuss the challenge of supplying power to implantable medical devices, with regard to the limitations of lithium battery technology and why implantable biofuel cells can be a promising alternative to provide the levels of power required for medical devices. In addition to the challenge of designing a biofuel cell that provides a stable level of sufficient power, the review highlights the biocompatibility and biofouling problems of implanting a biofuel cell that have a major impact on the availability of the substrates inside body that provide fuel for the biofuel cell. These physiological challenges and associated ethical considerations are essential to consider for biofuel cells that are designed to be implanted for long-term operation inside a living animal and eventually to human clinical applications. (C) 2018 The Authors. Published by Elsevier B.V.

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  • 22.
    Shleev, Sergey
    Malmö universitet, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV). National Research Centre “Kurchatov Institute”, Moscow, Russian Federation; A.N. Bach Institute of Biochemistry, Moscow, Russian Federation.
    Enzymatic Electrocatalysis of the Oxygen Reduction Reaction2018Ingår i: Encyclopedia of Interfacial Chemistry: Surface Science and Electrochemistry / [ed] Klaus Wandelt, Elsevier, 2018, s. 596-606Kapitel i bok, del av antologi (Refereegranskat)
    Abstract [en]

    Enzymatic reduction of oxygen is an important process in many living organizations; it is the key final step in the overall cycle of extracting energy from biofuels via oxidation. It is also a key step in the operation of technological contrivances, such as biosensors, biofuel cells, and biosupercapacitors. Issues like generation of harmful reactive oxygen species must be dealt with in living systems, as well as in technological applications. This article is focused on enzymatic electrocatalysis in the oxygen reduction reaction. Both direct-electron- and mediated-electron-transfer reactions are considered, nevertheless with some focus on the mediator-free approach. The active sites of enzymes, especially oxidoreductases, are intrinsically highly active. However, it appears that few enzymes can be fruitfully used for four-electron electroreduction of oxygen directly to water. Moreover, owing to limitations in oxygen availability and diffusion, oxygen bioelectroreduction has emerged as a rate-limiting step in overall fuel cycle processes and poses challenges in potential applications. Inadequate stability of redox enzymes is also an issue which is not often addressed. The potential and challenges for technological applications have been a driving force for research, but insight gained into biocatalyst structure/function is also mentioned where appropriate.

      

  • 23.
    Falk, Magnus
    et al.
    Malmö universitet, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV).
    Shleev, Sergey
    Malmö universitet, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV).
    Hybrid dual-functioning electrodes for combined ambient energy harvesting and charge storage: Towards self-powered systems.2018Ingår i: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 126, s. 275-291Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    In the last few years, there have been an increasing number of reports where different energy harvesters are directly combined with charge storing devices, based on dual-function electrodes able to convert and store electrical energy in the same volume. This includes (bio)fuel cells harvesting chemical energy, (bio)solar cells harvesting solar energy, tribo- and piezoelectric devices harvesting mechanical energy, and thermoelectrics harvesting thermal energy, which now have been intimately combined with batteries and electrochemical capacitors. These new types of hybrid electric devices show great promise especially for the design of self-powered electronics where an integrated hybrid power system is preferable to separated ones, capable of scavenging ambient energy and simultaneously store it and in this way increasing the efficiency and enabling further miniaturization. This paper details the recent emergence of hybrid energy systems, reviewing the progress made using widely different energy harvesting techniques, which have so-far not been described in a single body of work.

  • 24. Bobrowski, Tim
    et al.
    Gonzalez-Arribas, Elena
    Malmö universitet, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV).
    Ludwig, Roland
    Toscano, Miguel D.
    Shleev, Sergey
    Malmö universitet, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV).
    Schuhmann, Wolfgang
    Rechargeable, flexible and mediator-free biosupercapacitor based on transparent ITO nanoparticle modified electrodes acting in mu M glucose containing buffers2018Ingår i: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 101, s. 84-89Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    We present a transparent and flexible self-charging biosupercapacitor based on an optimised mediator- and membrane-free enzymatic glucose/oxygen biofuel cell. Indium tin oxide (ITO) nanoparticles were spray-coated on transparent conducting ITO supports resulting in a flocculent, porous and nanostructured electrode surface. By this, high capacitive currents caused by an increased electrochemical double layer as well as enhanced catalytic currents due to a higher number of immobilised enzyme molecules were obtained. After a chemical pretreatment with a silane derivative, bilirubin oxidase from Myrothecium verrucaria was immobilized onto the ITO nanostructured electrode surface under formation of a biocathode, while bioanodes were obtained by either immobilisation of cellobiose dehydrogenase from Corynascus thermophilus or soluble PQQ-dependent glucose dehydrogenase from Acinetobacter calcoaceticus. The latter showed a lower apparent K-M value for glucose conversion and higher catalytic currents at mu M glucose concentrations. Applying the optimised device as a biosupercapacitor in a discontinuous charge/discharge mode led to a generated power output of 0.030 mW/cm(2) at 50 mu M glucose, simulating the glucose concentration in human tears. This represents an enhancement by a factor of 350 compared to the power density obtained from the continuously operating biofuel cell with a maximum power output of 0.086 mu W/cm(2) under the same conditions. After 17 h of charging/discharging cycles a remarkable current enhancement was still measured. The entire device was transferred to flexible materials and applied for powering a flexible display showing its potential applicability as an intermittent power source in smart contact lenses.

  • 25. Alsaoub, Sabine
    et al.
    Ruff, Adrian
    Conzuelo, Felipe
    Ventosa, Edgar
    Ludwig, Roland
    Shleev, Sergey
    Malmö högskola, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV). Malmö högskola, Biofilms Research Center for Biointerfaces.
    Schuhmann, Wolfgang
    An Intrinsic Self-Charging Biosupercapacitor Comprised of a High-Potential Bioanode and a Low-Potential Biocathode2017Ingår i: ChemPlusChem, E-ISSN 2192-6506, Vol. 82, nr 4, s. 576-583Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    An intrinsic self-charging biosupercapacitor built on a unique concept for the fabrication of biodevices based on redox polymers is presented. The biosupercapacitor consists of a high-potential redox polymer based bioanode and a low-potential redox polymer based biocathode in which the potentials of the electrodes in the discharged state show an apparent potential mismatch E-anode > E-cathode and prevent the use of the device as a conventional biofuel cell. Upon charging, the potentials of the electrodes are shifted to more positive (cathode) and more negative (anode) values because of a change in the a(ox-)to-a(red) ratio within the redox polymer matrix. Hence, a potential inversion occurs in the charged state (E-anode < E-cathode) and an open circuit voltage of >0.4 V is achieved and the bio-device acts as a true biosupercapacitor. The bioanode consists of a novel specifically designed high-potential Os complex modified polymer for the efficient immobilization and electrical wiring of glucose converting enzymes, such as glucose oxidase and flavin adenine dinucleotide (FAD)-dependent glucose dehydrogenase. The cathodic side is constructed from a low-potential Os complex modified polymer integrating the O-2 reducing enzyme, bilirubin oxidase. The large potential differences between the redox polymers and the prosthetic groups of the biocatalysts ensure fast and efficient charging of the biodevice.

  • 26.
    Shleev, Sergey
    et al.
    Malmö högskola, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV).
    Gonzalez-Arribas, Elena
    Malmö högskola, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV).
    Falk, Magnus
    Malmö högskola, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV).
    Biosupercapacitors2017Ingår i: Current Opinion in Electrochemistry, E-ISSN 2451-9103, Vol. 5, nr 1, s. 226-233Artikel, forskningsöversikt (Refereegranskat)
    Abstract [en]

    This article reviews recent progress in the development of biosupercapacitors - supercapacitors fabricated using biological materials. In conventional biosupercapacitors the biomaterial serves as the pseudocapacitive component, while in self-charging biodevices the biocomponent also functions as the biocatalyst. The performance characteristics of biosupercapacitors are summarized and characterized in the perspective of the broader family of electric power devices, including biodevices. Self-charging biosupercapacitors show great promise in pulse-power delivery at the milliwatt level, typically greatly exceeding the capability of free-running bio-fuel and bio-solar cells. Thus, chemical biosupercapacitors might be suitable for powering a new generation of miniaturized electronic applications, including those intended for use in medical technology, while solar biodevices might be used as highly functional, but at the same time low-cost, environmentally friendly, and technically undemanding electric power sources.

  • 27. Bushnev, S.
    et al.
    Parunova, Y.
    Shleev, Sergey
    Malmö högskola, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV).
    Chemical biosupercapacitors for biomedical application2017Ingår i: The FEBS Journal, ISSN 1742-464X, E-ISSN 1742-4658, Vol. 284, nr S1, s. 207-207Artikel i tidskrift (Övrigt vetenskapligt)
    Abstract [en]

    The development of miniature autonomous bioelectronic devices that function in the human or animal internal environments is one of the most popular areas of bioelectronics. In recent works, a concept was developed for the creation of charge-storing fuel cells, or in other words self-charging supercapacitors based on (bio)electrodes with a dual function of generation and accumulation of electric charge and operating in both continuous and pulse modes. The main purpose of this work is to create a potentially implantable biodevice with a dual function of generation and accumulation of electrical charge on the basis of a membraneless nanobiocomposite biocathode with CNT/PANI/MvBOx composite material and a bioanode with GOx/AuNPs composite material, as well as investigation of their stability and efficiency in solutions close to the human blood. Nanobiocomposite materials are widely used as components of electronic devices for biomedical applications (biosensors, bio-fuel cells, biobataries, etc.) Modern bioelectronic devices based on nanocomposite materials can be used to influence organs and tissues, as well as for point delivery of drugs. Electrically conductive polymers are usually synthesized by chemical methods in an acid medium by oxidative polymerization of the monomer. This approach has a number of disadvantages, in particular, contamination of the final product with residual monomers and oxidant degradation products. Therefore, in this paper, electrochemical and enzymatic methods for the synthesis of electrically conducting polymers have been tested, which may be an alternative to chemical polymerization.

  • 28.
    Lamberg, P.
    et al.
    Department of Chemistry, University of Rochester, 14611 Rochester, NY, USA.
    Hamit-Eminovski, J.
    CMC Diabetes Formulation Development, Novo Nordisk, Novo Alle Bagsvaerd, 2880, Denmark.
    Toscano, M. D.
    Protein Engineering, Novozymes A/S, Bagsvaerd 2880, Denmark.
    Eicher-Lorka, O.
    Department of Organic Chemistry, Center for Physical Sciences and Technology, Sauletekio av. 3, LT-10257 Vilnius, Lithuania.
    Niaura, G.
    Department of Organic Chemistry, Center for Physical Sciences and Technology, Sauletekio av. 3, LT-10257 Vilnius, Lithuania.
    Arnebrant, Thomas
    Malmö högskola, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV).
    Shleev, Sergey
    Malmö högskola, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV).
    Ruzgas, Tautgirdas
    Malmö högskola, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV).
    Electrical activity of cellobiose dehydrogenase adsorbed on thiols: Influence of charge and hydrophobicity2017Ingår i: Bioelectrochemistry, ISSN 1567-5394, E-ISSN 1878-562X, Vol. 115, s. 26-32Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    The interface between protein and material surface is of great research interest in applications varying from implants, tissue engineering to bioelectronics. Maintaining functionality of bioelements depends greatly on the immobilization process. In the present study direct electron transfer of cellobiose dehydrogenase from Humicola insolens (HiCDH), adsorbed on four different self-assembled monolayers (SAMs) formed by 5-6 chain length carbon thiols varying in terminal group structure was investigated. By using a combination of quartz crystal micro balance with dissipation, ellipsometry and electrochemistry the formation and function of the HiCDH film was studied. It was found that the presence of charged pyridinium groups was needed to successfully establish direct electron contact between the enzyme and electrode. SAMs formed from hydrophilic charged thiols achieved nearly two times higher current densities compared to hydrophobic charged thiols. Additionally, the results also indicated proportionality between HiCDH catalytic constant and water content of the enzyme film. Enzyme films on charged pyridine thiols had smaller variations in water content and viscoelastic properties than films adsorbed on the more hydrophobic thiols. This work highlights several perspectives on the underlying factors affecting performance of immobilized HiCDH. (C) 2017 Elsevier B.V. All rights reserved.

  • 29. Scheiblbrandner, Stefan
    et al.
    Breslmayr, Erik
    Csarman, Florian
    Paukner, Regina
    Fuehrer, Johannes
    Herzog, Peter L.
    Shleev, Sergey V.
    Malmö högskola, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV).
    Osipov, Evgeny M.
    Tikhonova, Tamara V.
    Popov, Vladimir O.
    Haltrich, Dietmar
    Ludwig, Roland
    Kittl, Roman
    Evolving stability and pH-dependent activity of the high redox potential Botrytis aclada laccase for enzymatic fuel cells2017Ingår i: Scientific Reports, E-ISSN 2045-2322, Vol. 7, artikel-id 13688Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Fungal high redox potential laccases are proposed as cathodic biocatalysts in implantable enzymatic fuel cells to generate high cell voltages. Their application is limited mainly through their acidic pH optimum and chloride inhibition. This work investigates evolutionary and engineering strategies to increase the pH optimum of a chloride-tolerant, high redox potential laccase from the ascomycete Botrytis aclada. The laccase was subjected to two rounds of directed evolution and the clones screened for increased stability and activity at pH 6.5. Beneficial mutation sites were investigated by semi-rational and combinatorial mutagenesis. Fourteen variants were characterised in detail to evaluate changes of the kinetic constants. Mutations increasing thermostability were distributed over the entire structure. Among them, T383I showed a 2.6-fold increased half-life by preventing the loss of the T2 copper through unfolding of a loop. Mutations affecting the pH-dependence cluster around the T1 copper and categorise in three types of altered pH profiles: pH-type I changes the monotonic decreasing pH profile into a bell-shaped profile, pH-type II describes increased specific activity below pH 6.5, and pH-type III increased specific activity above pH 6.5. Specific activities of the best variants were up to 5-fold higher (13 U mg(-1)) than BaL WT at pH 7.5.

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  • 30. Di Bari, Chiara
    et al.
    Mano, Nicolas
    Shleev, Sergey
    Malmö högskola, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV). Malmö högskola, Biofilms Research Center for Biointerfaces.
    Pita, Marcos
    De Lacey, Antonio
    Halides inhibition of multicopper oxidases studied by FTIR spectroelectrochemistry using azide as an active infrared probe2017Ingår i: Journal of Biological Inorganic Chemistry, ISSN 0949-8257, E-ISSN 1432-1327, Vol. 22, nr 8, s. 1179-1186Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    An IR spectroelectrochem. study of Trametes hirsuta laccase and Magnaporthe oryzae bilirubin oxidase has been performed using azide, an inhibitor of multicopper oxidases, as an active IR probe incorporated into the T2​/T3 copper cluster of the enzymes. The redox potential-​controlled measurements indicate that N3-​ stretching IR bands of azide ion bound to the T2​/T3 cluster are only detected for the oxidized enzymes, confirming that azide only binds to Cu2+. Moreover, the process of binding​/dissocn. of azide ion is shown to be reversible. The interaction of halide anions, which also inhibit multicopper oxidases, with the active site of the enzymes was studied by measuring the changes in the azide FTIR bands. Enzymes inhibited by azide respond differently upon addn. of fluoride or chloride ions to the sample soln. inhibited by azide. Fluoride ions compete with azide for binding at one of the T2​/T3 Cu ions, whereas competition from chloride ions is much less evident.

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  • 31. Tapia, Cristina
    et al.
    Shleev, Sergey
    Malmö högskola, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV). Malmö högskola, Biofilms Research Center for Biointerfaces.
    Conesa, Jose Carlos
    De Lacey, Antonio L.
    Pita, Marcos
    Lactase-Catalyzed Bioelectrochemical Oxidation of Water Assisted with Visible Light2017Ingår i: ACS Catalysis, ISSN 2155-5435, E-ISSN 2155-5435, Vol. 7, nr 7, s. 4881-4889Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Here we present the modification of fluorinated tin oxide electrodes with In2S3, an n-type semiconductor chalcogenide that absorbs visible light (lambda <= 600 nm), and its further use as an active scaffold for laccase-catalyzed oxidation of water. Illumination of an FTO-In2S3-laccase electrode yields O-2 production at much lower applied potential in comparison to the previous example using the same laccase, where only electric energy was applied. The present system allows a diversification of the energy applied to accomplish the water splitting, taking a portion of it from the sun. This work is the first example where an enzyme other than PSII has been used in combination with visible light to biocatalyze O-2 evolution.

  • 32. Dagys, Marius
    et al.
    Laurynenas, Audrius
    Ratautas, Dalius
    Kulys, Juozas
    Vidziunaite, Regina
    Talaikis, Martynas
    Niaura, Gediminas
    Marcinkeviciene, Liucija
    Meskys, Rolandas
    Shleev, Sergey
    Malmö högskola, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV). Malmö högskola, Biofilms Research Center for Biointerfaces.
    Oxygen electroreduction catalysed by laccase wired to gold nanoparticles via the trinuclear copper cluster2017Ingår i: Energy & Environmental Science, ISSN 1754-5692, E-ISSN 1754-5706, Vol. 10, nr 2, s. 498-502Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Specific wiring of biocatalysts par excellence, viz. redox enzymes, to an electrode can be exploited in the fabrication of high-performance bioelectronic devices. Here we report oxygen electroreduction catalysed by Didymocrea sp. J6 laccase wired to gold nanoparticles via the trinuclear copper cluster. Bypassing the intramolecular electron transfer, which under certain conditions is the rate-limiting step of oxygen bioelectroreduction, has resulted in the fabrication of a high current density biocathode based on high-redox-potential laccase, which is able to operate in electrolytes with a broad pH range in the presence of high fluoride concentrations.

  • 33.
    Shleev, Sergey
    Malmö högskola, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV). Malmö högskola, Biofilms Research Center for Biointerfaces.
    Quo Vadis, Implanted Fuel Cell?2017Ingår i: ChemPlusChem, E-ISSN 2192-6506, Vol. 82, nr 4, s. 522-539Artikel, forskningsöversikt (Refereegranskat)
    Abstract [en]

    The road to safe and effective implantable electrical power devices has been long-and the goal has not been reached yet, although a certain amount of scientific and technological progress has been made. This brief review is focused on highlighting the stages of development of implanted fuel cells capable of providing electrical power for running implanted "personal electronics". The paper starts with early efforts to implant glucose-burning fuel cells in dogs, and ends with realistic attempts at interfacing a more sophisticated enzymatic glucose/oxygen fuel cell in an actual human blood stream. However, before that, the review deals with thermodynamic aspects of fuel cells, emphasizing the general advantages of these devices. Further steps, which are needed to realize the potential of this technology and which somehow differ from generally accepted ideas, are presented. These next steps are evaluated in the context of theoretically achievable abilities of implantable chemical power sources, which are not as great as many researchers might expect.

  • 34. Pankratova, Galina
    et al.
    Pankratov, Dmitry
    Malmö högskola, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV). Malmö högskola, Biofilms Research Center for Biointerfaces.
    Hasan, Kamrul
    Åkerlund, Hans-Erik
    Albertsson, Per-Åke
    Leech, Dónal
    Shleev, Sergey
    Malmö högskola, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV). Malmö högskola, Biofilms Research Center for Biointerfaces.
    Gorton, Lo
    Supercapacitive Photo-Bioanodes and Biosolar Cells: A Novel Approach for Solar Energy Harnessing2017Ingår i: Advanced Energy Materials, ISSN 1614-6832, E-ISSN 1614-6840, Vol. 7, nr 12Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    The concept of supercapacitive photo-bioanode and biosolar cell (photo-biosupercapacitor) for simultaneous solar energy conversion and storage is demonstrated for the first time. Exploiting the capacitive component significantly improves the electron transfer processes and allows the achievement of a current density of 280 µA cm−2 in the pulse mode.

  • 35.
    Gonzalez-Arribas, Elena
    et al.
    Malmö högskola, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV). Malmö högskola, Biofilms Research Center for Biointerfaces.
    Bobrowski, Tim
    Di Bari, Chiara
    Sliozberg, Kirill
    Ludwig, Roland
    Toscano, Miguel D.
    De Lacey, Antonio L.
    Pita, Marcos
    Schuhmann, Wolfgang
    Shleev, Sergey
    Malmö högskola, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV). Malmö högskola, Biofilms Research Center for Biointerfaces.
    Transparent, mediator- and membrane-free enzymatic fuel cell based on nanostructured chemically modified indium tin oxide electrodes2017Ingår i: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 97, s. 46-52Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    We detail a mediator- and membrane-free enzymatic glucose/oxygen biofuel cell based on transparent and nanostructured conducting supports. Chemically modified indium tin oxide nanoparticle modified electrodes were used to substantially increase the active surface area without significantly compromising transparency. Two different procedures for surface nanostructuring were employed, viz. spray-coating and drop-coating. The spray-coated biodevice showed superior characteristics as compared to the drop-coated enzymatic fuel cell, as a result of the higher nanostructured surface area as confirmed by electrochemical characterisation, as well as scanning electron and atomic force microscopy. Subsequent chemical modification with silanes, followed by the immobilisation of either cellobiose dehydrogenase from Corynascus thermophiles or bilirubin oxidase from Myrothecium verrucaria, were performed to obtain the bioanodes and biocathodes, respectively. The optimised biodevice exhibited an OCV of 0.67 V and power output of up to 1.4 mu W/cm(2) at an operating voltage of 0.35 V. This is considered a significant step forward in the field of glucose/oxygen membrane- and mediator-free, transparent enzymatic fuel cells.

  • 36.
    Pankratov, Dmitry
    et al.
    Malmö högskola, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV).
    Conzuelo, Felipe
    Pinyou, Piyanut
    Alsaoub, Sabine
    Schuhmann, Wolfgang
    Shleev, Sergey
    Malmö högskola, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV).
    A Nernstian Biosupercapacitor2016Ingår i: Angewandte Chemie International Edition, ISSN 1433-7851, E-ISSN 1521-3773, Vol. 55, nr 49, s. 15434-15438Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    We propose the very first "Nernstian biosupercapacitor", a biodevice based on only one redox polymer: poly(vinyl imidazole-​co-​allylamine)​[Os(bpy)​2Cl]​, and two biocatalysts. At the bioanode PQQ-​dependent glucose dehydrogenase reduces the Os3+ moieties at the polymer to Os2+ shifting the Nernst potential of the Os3+/Os2+ redox couple to neg. values. Concomitantly, at the biocathode the redn. of O2 by means of bilirubin oxidase embedded in the same redox polymer leads to the oxidn. of Os2+ to Os3+ shifting the Nernst potential to higher values. Despite the use of just one redox polymer an open circuit voltage of more than 0.45 V was obtained during charging and the charge is stored in the redox polymer at both the bioanode and the biocathode. By connecting both electrodes via a predefined resistor a high power d. is obtained for a short time exceeding the steady state power of a corresponding biofuel cell by a factor of 8.

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  • 37.
    Pankratov, Dmitry
    et al.
    Malmö högskola, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV).
    Ohlsson, Lars
    Malmö högskola, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV).
    Gudmundsson, Petri
    Malmö högskola, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV).
    Halak, Sanela
    Ljunggren, Lennart
    Malmö högskola, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV).
    Blum, Zoltan
    Malmö högskola, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV).
    Shleev, Sergey
    Malmö högskola, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV).
    Ex vivo electric power generation in human blood using an enzymatic fuel cell in a vein replica2016Ingår i: RSC Advances, E-ISSN 2046-2069, Vol. 6, nr 74, s. 70215-70220Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Here we report an enzymic fuel cell in a vein replica that generates sustained electricity, enough to power an e-​ink display, in an authentic human blood stream. We also detail a simple and safe approach for fuel cell evaluation under homeostatic conditions. Our results demonstrate proof-​of-​principle operation of a biocompatible and safe biodevice that could be implanted in superficial human veins, which we anticipate to be a starting point for more sophisticated investigations of personal sources of electricity.

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  • 38. Di Bari, Chiara
    et al.
    Goni-Urtiaga, Asier
    Pita, Marcos
    Shleev, Sergey
    Malmö högskola, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV).
    Toscano, Miguel
    Sainz, Raquel
    De Lacey, Antonio
    Fabrication of high surface area graphene electrodes with high performance towards enzymatic oxygen reduction2016Ingår i: Electrochimica Acta, ISSN 0013-4686, E-ISSN 1873-3859, Vol. 191, s. 500-509Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    High surface area graphene electrodes were prepd. by simultaneous electrodeposition and electroredn. of graphene oxide. The electrodeposition process was optimized in terms of pH and cond. of the soln. and the obtained graphene electrodes were characterized by XPS, FTIR spectroscopy, SEM and electrochem. methods (cyclic voltammetry and impedance spectroscopy)​. Electrodeposited electrodes were further functionalized to carry out covalent immobilization of two O-​reducing multicopper oxidases: laccase and bilirubin oxidase. The enzymic electrodes were tested as direct electron transfer based biocathodes and catalytic currents ≤1 mA​/cm2 were obtained. Finally, the mechanism of the enzymic O redn. reaction was studied for both enzymes calcg. the Tafel slopes and transfer coeffs.

  • 39. O'Conghaile, Peter
    et al.
    Falk, Magnus
    Malmö högskola, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV).
    MacAodha, Domhnall
    Yakovleva, Maria
    Gonaus, Christoph
    Peterbauer, Clemens
    Gorton, Lo
    Shleev, Sergey
    Malmö högskola, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV).
    Leech, Donal
    Fully Enzymatic Membraneless Glucose|Oxygen Fuel Cell That Provides 0.275 mA cm-?2 in 5 mM Glucose, Operates in Human Physiological Solutions, and Powers Transmission of Sensing Data2016Ingår i: Analytical Chemistry, ISSN 0003-2700, E-ISSN 1520-6882, Vol. 88, nr 4, s. 2156-2163Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Coimmobilization of pyranose dehydrogenase as an enzyme catalyst, osmium redox polymers [Os(4,​4'-​dimethoxy-​2,​2'-​bipyridine)​2(poly(vinylimidazole)​)​10Cl]​+ or [Os(4,​4'-​dimethyl-​2,​2'-​bipyridine)​2(poly(vinylimidazole)​)​10Cl]​+ as mediators, and carbon nanotube conductive scaffolds in films on graphite electrodes provides enzyme electrodes for glucose oxidn. The recombinant enzyme and a deglycosylated form, both expressed in Pichia pastoris, are investigated and compared as biocatalysts for glucose oxidn. using flow injection amperometry and voltammetry. In the presence of 5 mM glucose in phosphate-​buffered saline (PBS) (50 mM phosphate buffer soln., pH 7.4, with 150 mM NaCl)​, higher glucose oxidn. current densities, 0.41 mA​/cm2, are obtained from enzyme electrodes contg. the deglycosylated form of the enzyme. The optimized glucose-​oxidizing anode, prepd. using deglycosylated enzyme coimmobilized with [Os(4,​4'-​dimethyl-​2,​2'-​bipyridine)​2(poly(vinylimidazole)​)​10Cl]​+ and carbon nanotubes, was coupled with an oxygen-​reducing bilirubin oxidase on gold nanoparticle dispersed on gold electrode as a biocathode to provide a membraneless fully enzymic fuel cell. A max. power d. of 275 μW​/cm2 is obtained in 5 mM glucose in PBS, the highest to date under these conditions, providing sufficient power to enable wireless transmission of a signal to a data logger. When tested in whole human blood and unstimulated human saliva max. power densities of 73 and 6 μW​/cm2 are obtained for the same fuel cell configuration, resp.

  • 40. Di Bari, Chiara
    et al.
    Shleev, Sergey
    Malmö högskola, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV).
    De Lacey, Antonio
    Pita, Marcos
    Laccase-​modified gold nanorods for electrocatalytic reduction of oxygen2016Ingår i: Bioelectrochemistry, ISSN 1567-5394, E-ISSN 1878-562X, Vol. 107, s. 30-36Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    The multicopper oxidase Trametes hirsuta laccase (ThLc) served as a bioelectrocatalyst on nanostructured cathodes. Nanostructuring was provided by gold nanorods (AuNRs)​, which were characterized and covalently attached to electrodes made of low-​d. graphite. The nanostructured electrode was the scaffold for covalent and oriented attachment of ThLc. The bioelectrocatalytic currents measured for oxygen redn. were as high as 0.5 mA​/cm2 and 0.7 mA​/cm2, which were recorded under direct and mediated electron transfer regimes, resp. The exptl. data were fitted to math. models showing that when the O2 is bioelectroreduced at high rotation speed of the electrode the heterogeneous electron transfer step is the rate-​liming stage. The electrochem. measurement hints a wider population of non-​optimally wired laccases than previously reported for 5-​8 nm size Au nanoparticle-​modified electrode, which could be due to a larger size of the AuNRs when compared to the laccases as well as their different crystal facets.

  • 41.
    Shleev, Sergey
    et al.
    Malmö högskola, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV).
    Andoralov, Viktor
    Pankratov, Dmitry
    Malmö högskola, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV).
    Falk, Magnus
    Malmö högskola, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV).
    Aleksejeva, Olga
    Malmö högskola, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV).
    Blum, Zoltan
    Malmö högskola, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV).
    Oxygen Electroreduction Versus Bioelectroreduction: Direct Electron Transfer Approach2016Ingår i: Electroanalysis, ISSN 1040-0397, E-ISSN 1521-4109, Vol. 28, nr 10, s. 2270-2287Artikel, forskningsöversikt (Refereegranskat)
    Abstract [en]

    A review. Being inspired by a very recent review entitled: "Electrocatalysis and bioelectrocatalysis - Distinction without a difference" and following the general approach employed by Prof. Dr. Schuhmann, in the current work we point to the similarities and differences between oxygen electroredn. and bioelectroredn. processes. To addnl. distinguish our paper from the recent review we touch on different bioelements, such as redox proteins and living cells, but we still keep a special emphasis on oxidoreductases, biocatalysts par excellence. Moreover, we also exclusively focus on oxygen electroredn. based on direct electron transfer reactions. On the one hand, we corroborate the previously made conclusion regarding intrinsically high activity of the active sites of biol. catalysts, esp. redox enzymes, which results in mass transfer and heterogeneous electron transfer limited currents from oxygen reducing bioelectrodes. On the other hand, we disagree with the statements regarding the exceptionality of precious metal catalysts, and the notion of a huge trade-​off between high activity and stability of non-​precious metal catalysts and bioelements. We show that the activity and stability of noble metal based cathodes is very far from perfect, esp. when these electrodes operate in complex electrolytes, such as physiol. fluids, e.g. human blood.

  • 42.
    Parunova, Yulia
    et al.
    National Research Institute “Kurchatov Institute,”, Moscow, 123182, Russia.
    Bushnev, Sergey
    National Research Institute “Kurchatov Institute,”, Moscow, 123182, Russia.
    Gonzalez-Arribas, Elena
    Malmö högskola, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV). Malmö högskola, Biofilms Research Center for Biointerfaces.
    Falkman, Peter
    Malmö högskola, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV). Malmö högskola, Biofilms Research Center for Biointerfaces.
    Lipkin, Aleksey
    National Research Institute “Kurchatov Institute,”, Moscow, 123182, Russia.
    Popov, Vladimir
    National Research Institute “Kurchatov Institute,”, Moscow, 123182, Russia.
    Shleev, Sergey
    Malmö högskola, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV). Malmö högskola, Biofilms Research Center for Biointerfaces. National Research Institute “Kurchatov Institute,”, Moscow, 123182, Russia.
    Pankratov, Dmitry
    Malmö högskola, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV). Malmö högskola, Biofilms Research Center for Biointerfaces. National Research Institute “Kurchatov Institute,”, Moscow, 123182, Russia.
    Potentially implantable biocathode with the function of charge accumulation based on nanocomposite of polyaniline​/carbon nanotubes2016Ingår i: Russian journal of electrochemistry, ISSN 1023-1935, E-ISSN 1608-3342, Vol. 52, nr 12, s. 1166-1171Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    A potentially implantable biocathode with the function of charge accumulation based on a nanobiocomposite including multiwall carbon nanotubes, polyaniline, and bilirubin oxidase is developed. The regularities of the functioning of the obtained electrode are studied in air-​satd. phosphate buffer soln., pH 7.4 (PB)​, and also in phosphate buffer soln. contg. redox-​active blood components (BMB)​. The open circuit potential of the biocathode is 0.33 and 0.08 V vs. the SCE in PB and BMB, resp.; it is completely restored after at least three self-​charge​/discharge cycles with connection to resistors with different resistance. Bioelectrocatalytic c.d. of oxygen redn. is 0.50 and 0.42 mA cm-​2 with the residual activity of 78 and 60​% of the initial value after 12 h of continuous operation in PB at 25°C and in BMB at 37°C, resp.

  • 43.
    Gonzalez-Arribas, Elena
    et al.
    Malmö högskola, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV).
    Aleksejeva, Olga
    Malmö högskola, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV).
    Bobrowski, Tim
    Toscano, Miguel
    Gorton, Lo
    Schuhmann, Wolfgang
    Shleev, Sergey
    Malmö högskola, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV).
    Solar biosupercapacitor2016Ingår i: Electrochemistry communications, ISSN 1388-2481, E-ISSN 1873-1902, Vol. 74, s. 9-13Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Here we report on an entirely new kind of bioelectronic device - a solar biosupercapacitor, which is built from a dual-​feature photobioanode combined with a double-​function enzymic cathode. The self-​charging biodevice, based on transparent nanostructured indium tin oxide electrodes modified with biol. catalysts, i.e. thylakoid membranes and bilirubin oxidase, is able to capacitively store electricity produced by direct conversion of radiant energy into elec. energy. When self-​charged during 10 min, using ambient light only, the biosupercapacitor provided a max. of 6 mW m-​ 2 at 0.20 V.

  • 44.
    Pankratov, Dmitry
    et al.
    Malmö högskola, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV).
    Gonzalez-Arribas, Elena
    Malmö högskola, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV).
    Blum, Zoltan
    Malmö högskola, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV).
    Shleev, Sergey
    Malmö högskola, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV).
    Tear Based Bioelectronics2016Ingår i: Electroanalysis, ISSN 1040-0397, E-ISSN 1521-4109, Vol. 28, nr 6, s. 1250-1266Artikel, forskningsöversikt (Refereegranskat)
    Abstract [en]

    A review. This work provides an overview of the recent advances in the field of tear-​based wearable electrochem. biodevices, including non-​invasive biosensors, biol. fuel cells and biosupercapacitors. Contact lenses are attractive platforms for fabricating non-​invasive self-​contained gadgets for different applications, starting from devices with casual or mundane purposes only, like personalized smart lenses with direct (invisible for others) displays, and ending with biomedical devices for continuous fitness status and​/or health care monitoring. Key requirements and challenges that confront researchers in this exciting area are discussed.

  • 45.
    Gonzalez-Arribas, Elena
    et al.
    Malmö högskola, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV).
    Pankratov, Dmitry
    Malmö högskola, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV).
    Gounel, Sebastien
    Mano, Nicolas
    Blum, Zoltan
    Malmö högskola, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV).
    Shleev, Sergey
    Malmö högskola, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV).
    Transparent and Capacitive Bioanode Based on Specifically Engineered Glucose Oxidase2016Ingår i: Electroanalysis, ISSN 1040-0397, E-ISSN 1521-4109, Vol. 28, nr 6, s. 1290-1297Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Here the authors detail an optimized transparent capacitive glucose oxidizing bioanode, capable of supplying current densities of 10 μA cm-​2 at applied potentials of 0.1 V-​0.2 V vs. SCE, when continuously performing in a simple phosphate buffer, pH 7.4 and artificial human tears, both with a glucose concn. of 0.05 mM only. When operating in pulse mode, the bioanode was able to deliver current densities ≤21 μA cm-​2 at the beginning of the pulse with 571 μC cm-​2 total charges stored. The biogenic part of the enzymic device was a recombinant glucose oxidase mutant from Penicillium amagasakiense with high catalytic efficiency towards glucose, up to 14.5x104 M-​1 s-​1. The nonbiogenic part of the anodic system was based on a poly(3,​4-​ethylenedioxythiophene)​-​graphene nanocomposite, as a highly capacitive component with a capacitance d. in the 1 mF cm-​2 range, multi-​walled carbon nanotubes, as an addnl. nanostructuring element, and a conductive org. complex, as an electron shuttle between the redox enzyme and the electrode surface. The bioanode could potentially serve as a prototype of a double-​function enzymic anode for hybrid elec. power biodevices, energizing smart contact lenses.

  • 46.
    Shleev, Sergey
    et al.
    Malmö högskola, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV). Malmö högskola, Biofilms Research Center for Biointerfaces.
    Bergel, Alain
    Gorton, Lo
    Biological fuel cells: Divergence of opinion2015Ingår i: Bioelectrochemistry, ISSN 1567-5394, E-ISSN 1878-562X, Vol. 106, nr Part A, s. 1-2Artikel i tidskrift (Övrigt vetenskapligt)
    Abstract [en]

    Editorial

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  • 47. Suyatin, Dmitry B.
    et al.
    Sundberg, Richard
    Malmö högskola, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV). Malmö högskola, Biofilms Research Center for Biointerfaces.
    Maximov, Ivan
    Shleev, Sergey
    Malmö högskola, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV). Malmö högskola, Biofilms Research Center for Biointerfaces.
    Montelius, Lars
    Concept for assembling individual nanostructure-based components into complex devices2015Ingår i: Journal of Vacuum Science and Technology B: Nanotechnology and Microelectronics, ISSN 2166-2746, E-ISSN 2166-2754, Vol. 33, artikel-id 062202Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Minute electronic (bio)devices will likely play an increasingly important role in everyday life and beyond, as overall device size often limits device functionality and applicability, a factor especially critical for brain implants. Recent progress in micro- and nanoelectronics has enabled the production of nanoscale electronic components; however, overall device size is often defined by technical and technological limitations, in particular, the ability to combine heterogeneous components made using incompatible processes on different substrates. Here, the authors suggest and evaluate a concept and approach aimed at the direct three-dimensional assembly of individual nanoscale-based components into complex devices for brain implants. They demonstrate this assembly possibility via the transfer of free-standing GaP nanowires, as well as test devices made of gold film which exhibit good quality electrical contacts. The key features essential for such a functional assembly process are discussed. The authors expect this approach to be generic and to enable the development of complex minute electronic (bio)devices based on nanoscale components. The proposed type of assembly may be especially beneficial for devices with strict size constraints, such as implantable neural interfaces.

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  • 48. Zeng, Ting
    et al.
    Pankratov, Dmitry
    Malmö högskola, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV).
    Falk, Magnus
    Malmö högskola, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV).
    Leimkühler, Silke
    Shleev, Sergey
    Malmö högskola, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV).
    Wollenberger, Ulla
    Miniature direct electron transfer based sulphite/oxygen enzymatic fuel cells2015Ingår i: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 66, s. 39-42Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    A direct electron transfer (DET) based sulphite/oxygen biofuel cell is reported that utilises human sulphite oxidase (hSOx) and Myrothecium verrucaria bilirubin oxidase (MvBOx) and nanostructured gold electrodes. For bioanode construction, the nanostructured gold microelectrodes were further modified with 3,3′-dithiodipropionic acid di(N-hydroxysuccinimide ester) to which polyethylene imine was covalently attached. hSOx was adsorbed onto this chemically modified nanostructured electrode with high surface loading of electroactive enzyme and in presence of sulphite high anodic bioelectrocatalytic currents were generated with an onset potential of 0.05 V vs. NHE. The biocathode contained MvBOx directly adsorbed to the deposited gold nanoparticles for cathodic oxygen reduction starting at 0.71 V vs. NHE. Both enzyme electrodes were integrated to a DET-type biofuel cell. Power densities of 8 and 1 μW cm−2 were achieved at 0.15 V and 0.45 V of cell voltages, respectively, with the membrane based biodevices under aerobic conditions.

  • 49.
    Pankratov, Dmitry
    et al.
    Malmö högskola, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV). Malmö högskola, Biofilms Research Center for Biointerfaces.
    González Arribas, Elena
    Malmö högskola, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV). Malmö högskola, Biofilms Research Center for Biointerfaces.
    Parunova, Yulia M.
    Gorbacheva, Marina A.
    Zeyfman, Yulia S.
    Kuznetsov, Sergey V.
    Lipkin, Aleksey
    Shleev, Sergey
    Malmö högskola, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV). Malmö högskola, Biofilms Research Center for Biointerfaces.
    New nanobiocomposite materials for bioelectronic devices2015Ingår i: Acta Naturae, ISSN 2075-8251, Vol. 7, nr 1, s. 98-101Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    We have developed and synthesized nanobiocomposite materials based on graphene, poly(3,4-ethylenedioxythiophene), and glucose oxidase immobilized on the surface of various nanomaterials (gold nanoparticles and multi-walled carbon nanotubes) of different sizes (carbon nanotubes of different diameters). Comparative studies of the possible influence of the nanomaterial’s nature on the bioelectrocatalytic characteristics of glucose-oxidizing bioanodes in a neutral phosphate buffer solution demonstrated that the bioelectrocatalytic current densities of nanocomposite-based bioanodes are only weakly dependent on the size of the nanomaterial and are primarily defined by its nature. The developed nanobiocomposites are promising materials for new bioelectronic devices due to the ease in adjusting their capacitive and bioelectrocatalytic characteristics, which allows one to use them for the production of dual-function electrodes: i.e., electrodes which are capable of generating and storing electric power simultaneously.

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    fulltext
  • 50.
    Pankratov, Dmitry
    et al.
    Malmö högskola, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV). Malmö högskola, Biofilms Research Center for Biointerfaces.
    Sundberg, Richard
    Sotres, Javier
    Malmö högskola, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV). Malmö högskola, Biofilms Research Center for Biointerfaces.
    Suyatin, Dmitry B.
    Maximov, Ivan
    Shleev, Sergey
    Malmö högskola, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV). Malmö högskola, Biofilms Research Center for Biointerfaces.
    Montelius, Lars
    Scalable, high performance, enzymatic cathodes based on nanoimprint lithography2015Ingår i: Beilstein Journal of Nanotechnology, ISSN 2190-4286, Vol. 6, s. 1377-1384Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Here we detail high performance, enzymatic electrodes for oxygen bio-electroreduction, which can be easily and reproducibly fabricated with industry-scale throughput. Planar and nanostructured electrodes were built on biocompatible, flexible polymer sheets, while nanoimprint lithography was used for electrode nanostructuring. To the best of our knowledge, this is one of the first reports concerning the usage of nanoimprint lithography for amperometric bioelectronic devices. The enzyme (Myrothecium verrucaria bilirubin oxidase) was immobilised on planar (control) and artificially nanostructured, gold electrodes by direct physical adsorption. The detailed electrochemical investigation of bioelectrodes was performed and the following parameters were obtained: open circuit voltage of approximately 0.75 V, and maximum bio-electrocatalytic current densities of 18 µA/cm2 and 58 µA/cm2 in air- saturated buffers versus 48 µA/cm2 and 186 µA/cm2 in oxygen-saturated buffers for planar and nanostructured electrodes, respect- ively. The half-deactivation times of planar and nanostructured biocathodes were measured to be 2 h and 14 h, respectively. The comparison of standard heterogeneous and bio-electrocatalytic rate constants showed that the improved bio-electrocatalytic performance of the nanostructured biocathodes compared to planar biodevices is due to the increased surface area of the nanostructured electrodes, whereas their improved operational stability is attributed to stabilisation of the enzyme inside nanocavities.

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