Histoner är en familj av proteiner som bildar nukleosomer vid packning av DNA i cellkärnan. Komplexet mellan histoner och DNA benämns kromatin. Utöver histoner består kromatinet av proteiner som är icke-histoner vilket bidrar till kromatinstabilitet samt genaktivitet. Histoner, med molekylvikter om 11-21 kDa, omfattar fem huvudklasser: H1, H2A, H2B, H3, H4, som bildar ett komplex med varandra genom jonbindning. Histonkomplexet består av (H2A-H2B) som är flankerade av (H3-H4) tetramerer. Eftersom histoner består av basiska aminosyror såsom arginin och lysin är de positivt laddade, vilket underlättar bindningen med DNA som är negativt laddat. Histoner betraktas som antimikrobiella peptider (AMPs) för att de har förmåga att neutralisera bakteriellt endotoxin. Syftet med studien var att rena upp histoner från vetegroddar och karaktärisera dessa, samt studera deras effekt på bakterietillväxt. Vetegroddar användes eftersom de är rika på kromatin. Metoden för att rena upp histoner var baserad på låg jonstyrka ochutnyttjande av tre olika buffertar samt syra extraktion av kromatin med svavelsyra. För att karaktärisera histoner användes SDS-PAGE elektrofores och för att studera deras bakteriella hämning användes E. coli bakterier. Resultatet visade på ett lågt utbyte av histoner, men dock kunde olika histoners huvudklasser separeras och karaktäriseras genom SDS-PAGE. Vissa prover visade på en bakteriehämmande effekt. Optimering av extraktionen är nödvändig för att öka utbytet och därmed bättre kunna studera histonernas antibakteriella effekt. Förmodligen är det homogeniseringen som begränsar utbytet, och därtill bör man också se över hanteringen av syra extraktet för att förhindra aggregering.

Nyckelord: Antimikrobiella peptider, DNA, histoner, jonstyrka, kromatin, syraextraktion, vetegroddar, upprening

Huden är kroppens största organ, det är mycket komplext och har flera funktioner. Ett av hudens primära funktioner är att förhindra vattenförlust genom att hålla kvar vatten så att huden kan fungera i torra miljöer. Det yttersta lagret av huden stratum corneum (SC) förhindrar vattenförlust genom att återfukta med natural moisturizing factors (NMFs). I detta projekt inkuberades HaCaT celler med vanligt förekommande hudvårdsingredienser (urea, glycerol, transcutol, salicylsyra och polyetylenglykol 4000 Da (PEG-4000)) för att utvärdera deras inverkan på cellviabilitet, genom MTT proliferation assay och genuttryck, med qPCR. Sambandet mellan viabilitet, genuttryck och osmotisk stress studerades också. Molekylerna visade en dosberoende minskning av cellviabilitet då det osmotiska trycket ökade. Ett resultat var att transcutol gav en skyddande effekt mot koncentrationer där osmotiskt tryck bör skada cellen. PEG-4000 med en koncentration av 10 % (w/v) visade en uppreglering av elongation of very long chain fatty acid (ELOVL-4). Vid tillsats av 10 mM transcutol var cellviabiliteten >100 % och genuttrycket av serin palmitoyltransferas (SPT) lågt. Teoretiskt sätt borde det varit en uppreglering av SPT då cellerna var metabolt aktiva. Sammanfattningsvis är viabilitet och genuttryck troligen relaterade till osmotisk stress men kan ytterligare analyseras med digital holographic microscopy (DHM) och Western blot.

Vitamin K is a fat soluble compound which is synthesized by the gut microbiota and produced in many tissues within the body. Considering its role in the liver as a cofactor for gamma carboxylase enzymes, treatment of dark circles and pigments under the eye among others. It is clear that is some circumstances vitamin K has to cross biological barriers, particularly, when the vitamin is produced by microbiota in the intestine or applied topically on skin. Thus it is important to develop methods that allow studies of vitamin K permeability through the skin including its participation in redox reactions and transdermal permeability. Taking into account that transdermal permeability is strongly limited for high molecular weight compounds, i.e., compounds with higher than 500Da, the study was conducted with vitamin K of  lower molecular weight. Specifically vitamin K4 model, i.e., 1,4-dihydroxy-2 naphthoic acid, with molecular weight of 204g/mol. Vitamin K4 is suitable for this kind of study , because it can work as reducing (antioxidant) compound as well as has relatively beneficial physicochemical characteristics for transdermal permeability. Permeability studies were conducted with skin covered oxygen electrode and franz diffusion cell. Data from measurements were analyzed to estimate diffusion coefficients, apparent Michaelis-Menten constants and flux of a vitamin K4 model whilst contribution of different permeability pathways was determined theoretically.

På Nuklearmedicin, VO Bild och Funktion på Skånes universitetssjukhus i Malmö sker dagligen administrering med fluor-18 (18F) märkta läkemedel, vilket medför exponering för joniserande strålning till personal i verksamheten. Strålskyddsförordningen i Sverige, har riktlinjer för dosgränser för att förhindra eventuella negativa hälsoeffekter på både allmänheten och arbetstagare som är aktiva i verksamhet med joniserande strålning. Syftet med studien var att studera om det föreligger signifikant skillnad i stråldos till personalens fingrar respektive kropp efter administrering med 18F märkt läkemedel, beroende på när borttagning av perifer venkateter (PVK) sker. Mätningarna utfördes direkt efter administrering av 18F-märkt läkemedel samt efter bildtagning. I studien utfördes 44 mätningar på 11 av personalen vid nuklearmedicinska avdelningen, varav 22 mätningar utfördes efter administration av 18F-märkt läkemedel och 22 mätningar efter bildtagning. Efter 22 mätningar visar resultaten ett medelvärde av den ekvivalenta dosen till fingrarna direkt efter injektion på 0,20 μSv/MBq och efter bildtagning på 0,18 μSv/MBq. Medelvärdet av den uppmätta effektiva dosen på 0,004 μSv/MBq efter injektion och 0,003 μSv/MBq efter bildtagning är i samma storleksordning. Ett Mann-Whitney U-test redovisade ett p-värde på >0,05, vilket tyder på att det inte föreligger någon signifikant skillnad i stråldos mellan de två olika metoderna vid borttagning av PVK. 

Kolorektalcancer är den fjärde vanligaste cancerrelaterade orsaken till dödsfall i världen. År 2020 diagnostiserades totalt 1,93 miljoner nya fall varav 60% diagnostiserades med koloncancer. Cirka 30–40% av patienter med koloncancer har en mutation i KRAS-genen. Denna mutation har associerats med en sämre överlevnad, ökad tumör-aggressivitet samt resistens mot utvalda behandlingsstrategier. Det finns för närvarande ingen effektiv läkemedelsinriktad-behandling mot KRAS-muterad koloncancer. Däremot har möjligheten att utreda effekten av läkemedelsbehandling på koloncancer ökat, efter att KRAS-inhibitorn ISA-2011B har upptäckts. Studiens syfte är att analysera effekten av ISA-2011B vid både enkel och kombinerad behandling med Erlotinib och Docetaxel, på humana koloncancercellinjer. Cancercellinjerna HCT116 (KRAS-muterad) och HT29 (BRAF-muterad) användes för jämförelse av cellproliferation vid behandling av de nämnda läkemedlen samt ISA-2011B. Analys av cellinjernas proliferation utfördes med hjälp av MTS-analys. Den signifikanta skillnaden vad gäller effekten av ISA-2011B i jämförelse med DMSO kontrollen, fastställdes med ett 2 sidigt t-test. Studien har visat en signifikant effekt av ISA-2011B på cellinjerna HCT116 och HT29. Behandling med endast ISA-2011B påvisade en signifikant effekt på cancercellinjerna och minskade deras proliferation. Dock var denna effekt oförändrad i kombination med Docetaxel eller Erlotinib. Däremot visade ISA-2011B ha störst effekt på HCT116 vad gäller minskad cell-proliferation i jämförelse med HT29. Sammanfattningsvis kommer studiens resultat att bidra med ökad förståelse kring effekten av ISA-2011B på koloncancercellinjerna. Mer omfattande studier på sfäroider samt xenograftmöss behöver utföras med positiva resultat för att ISA-2011B ska kunna bedömas som ett fungerande läkemedel och därmed kunna implementeras på patienter med KRAS-muterad koloncancer.

Background Medication discrepancies in care transitions and medication non-adherence are problematic. Few interventions consider the entire process, from the hospital to the patient’s medication use at home.

Aim In preparation for randomised controlled trials (RCTs), this study aimed (1) to investigate the feasibility of recruitment and retention of patients and data collection to reduce medication discrepancies at discharge and improve medication adherence and (2) to explore the outcomes of the interventions.

Method Participants were recruited from a hospital and a residential area. Hospital patients participated in a pharmacist-led intervention to establish a correct medication list upon discharge and a follow-up interview two weeks post-discharge. All participants received a person-centred adherence intervention for three to six months. Discrepancies in the medication lists, the Beliefs about Medicines Questionnaire (BMQ-S), and the Medication Adherence Report Scale (MARS-5) were assessed.

Results Of 87 asked to participate, 35 were included, and 12 completed the study. Identifying discrepancies, discussing discrepancies with physicians, and performing follow-up interviews were possible. Conducting the adherence intervention was also possible using individual health plans for medication use. Among the seven hospital patients, 24 discrepancies were found. Discharging physicians agreed that all discrepancies were errors, but only ten were corrected in the discharge information. Ten participants decreased their total BMQ-S concern scores, and seven increased their total MARS-5 scores.

Conclusion Based on this study, conducting the two RCTs separately may increase the inclusion rate. Data collection was feasible. Both interventions were feasible in many aspects but need to be optimised in upcoming RCTs.

Algae extracts are used as cosmetic products and used as additives in specific foods because of their antioxidant activity. Reactive oxygen species (ROS) such as hydrogen peroxide and superoxide radicals are toxic on the skin and can be scavenged by antioxidants, which are abundant in microalgae. Antioxidant substances protect the skin against external factors such as ultraviolet light (UV). Many creams that are concerned with treating the skin have antioxidant molecules. Researchers are performing many studies to achieve natural and non-chemical skin maintenance for the skin. Conducting in vivo studies to analyze the antioxidant potential of extracts on skin needs ethical permission to recruit a specific number of people. In this study, pig ear skin was used with the help of a skin-membrane-covered oxygen electrode (SCOE) to analyze the antioxidant effect of an extract from the microalgae Chlamydomonas reinhardtii. A study proved that this method was effective in measuring other antioxidants. Hydroquinone was used as a control to see if the system worked correctly. Hydroquinone showed that it could penetrate the skin and give antioxidant activity. When the algae extract was used, the same effect as for hydroquinone could not be detected. With a 2,2-diphenylpicrylhydrazyl (DPPH) assay, the algae extract was analyzed for its antioxidant capacity, and the assay revealed a positive antioxidant effect of the extract. The spectrophotometric measurement of the amount of bioactive antioxidant molecules in the extract in different solutions suggested that the one resuspended in ethanol presented a higher amount of carotenoids and chlorophylls than the extract resuspended in an aqueous buffer. Additional research will be needed to characterize the antioxidant potential of the extract from the microalgae Chlamydomonas reinhardtii.

This thesis is focused on bioelements for biological electric power sources,specifically, on blue copper proteins with and without an intrinsic biocatalyticactivity, i.e. ability to reduce oxygen directly to water. These proteins, viz. differentlaccases, ceruloplasmin, and rusticyanin, were characterised in detailand employed for the construction of both self-charging and conventional biosupercapacitors.First, similarities and particularities of oxygen electroreductionvs. bioelectroreduction were reviewed. Moreover, being a promising candidatefor the construction of autotolerant implantable biocathodes, the electrochemistryof human ceruloplasmin was revisited. For the first time, a clearbioelectrocatalytic reduction of oxygen on ceruloplasmin modified electrodeswas shown. Second, computational design combined with directed evolutionresulted in a high redox potential mutated laccase, GreeDo, with increased redoxpotential of the T1 site, increased activity towards high redox potentialmediators, as well as enhanced stability. Third, GreeDo was electrochemicallycharacterised in detail. The mutant exhibited higher open circuit potentialvalues and onset potentials for oxygen bioelectroreduction compared to the parental laccase, OB-1. Moreover, the operational stability of GreeDo modifiedgraphite electrodes was found to be more than 2 h in a decidedly acidicelectrolyte, in agreement with the extended operational and storage stabilitiesof the enzyme in acidic solutions. Fourth, multi-cell single-electrolyte glucose/oxygen biodevices with adjustable open-circuit and operating voltages,which are independent on the difference in equilibrium redox potentials of thetwo redox couples, gluconolactone/glucose and oxygen/water, viz. 1.18 V, butdependent on the number of half-cells in the biodevice construction, were designedand tested. The biodevices were made from tubular graphite electrodeswith electropolymerised poly(3,4-ethylenedioxythiophene) modified withTrametes hirsuta laccase and Neurospora crassa cellobiose dehydrogenase as the cathodic and anodic biocatalysts, respectively. Due to the interplay betweenfaradaic and non-faradaic electrochemical processes, as well as betweenionic and electronic conductivities, the open-circuit voltage of the self-chargedbiodevice is extraordinarily high, reaching 3 V, when seven biosupercapacitorsoperating in a common electrolyte were connected in series. Moreover,glucose/oxygen biodevices could be externally discharged at an operatingvoltage exceeding the maximal limiting open-circuit value of 1.24 V for thecomplete glucose oxidation. Last but not least, a conventional biosupercapacitor,i.e. a biodevice lacking self-charging ability, was composed of Acidithiobacillusferrooxidans rusticyanin modified gold electrodes. The complete biodevicesas well as separate electrodes were thoroughly characterised electrochemically.The symmetrical biosupercapacitor based on two identical goldelectrodes modified with rusticyanin is able to capacitively store electricityand deliver electric power, accumulated mostly in the form of biopseudocapacitance,when charged and discharged externally.

Electrochemical characterization of the GreeDo variant of a high redox potential fungal laccase obtained by laboratory evolution together with computer-guided mutagenesis, in comparison to its parental variety (the OB-1 mutant), is presented. Both laccases, when immobilized on graphite electrodes either by direct physical adsorption or covalently attached via gold nanoparticles, were capable of both non-mediated and mediator-based bioelectroreduction of molecular oxygen at low overpotentials. GreeDo exhibited higher open circuit potential values and onset potentials for oxygen bioelectroreduction compared to OB-1. However, even though in homogeneous catalysis GreeDo outperforms OB-1 in terms of turnover numbers and catalytic efficiency, when exposed to high redox potential substrates, direct electron transfer based bioelectrocatalytic currents of GreeDo and OB-1 modified electrodes were similar. High operational stability of freely diffusing GreeDo and also the immobilized enzyme in the acidic electrolyte was registered, in agreement with high storage stability of GreeDo in acidic solutions.

Direct transformation of solar energy into electrical energy by means of biological photosynthesis is considered as an attractive option for sustainable electrical energy production. Thylakoid membranes, the site of photosynthesis, are regarded as a promising biological material for the development of photoelectric biodevices, which produce electrical power consuming only light energy as oxygen evolves at photobioanode upon irradiation and biocathode converts it back to water. Therefore, in this work dual-feature photobioanode based on nanoimprinted gold substrates modified with thylakoids in combination with a capacitive part made of a planar gold substrate coated with a conductive polymer was designed and evaluated, providing open-circuit potential of -0.21 V vs. Ag vertical bar AgCl vertical bar KClsat and a capacitance of ca. 60 F m(-2) both at ambient light and artificial illumination of 400 W m(-2). Combination of thylakoid based dual-feature photobioanode with bilirubin oxidase modified transparent and capacitive indium tin oxide biocathode resulted in a photoelectric biosupercapacitor with remarkable characteristics at ambient light, viz. an open-circuit voltage as high as 0.74 V, which was stable upon charge-discharge cycles during ca. 2 h.

As the global energy demand continues to increase, the interest in photosynthetic energy conversion is growing accordingly. Chloroplasts, photosynthetic organelles present in plants and algae, are attractive candidates for construction of bio solar cells; however, they have been less studied because of their complex membrane system, which restricts electrochemical communication with an electrode surface. Nevertheless, in this work photobioanodes based on planar and nanoimprinted gold substrates modified with chloroplasts were designed and evaluated. Apparently, nanoimprint lithography contributed to higher photocurrent densities, not only owing to the enlarged real surface area, but also due to boosting electrochemical communication between the photosynthetic organelles and the electrode. Combining chloroplast-modified nanoimprinted gold electrodes with a capacitive part made of a planar gold substrate, coated with a conductive polymer, resulted in a dual-feature photobioanode providing a lower open-circuit potential, i. e., -0.11 V vs. Ag|AgCl|KClsat, and an enhanced capacitance of ca. 37 F m(-2) upon illumination of 400 W m(-2).

High-performance autotolerant bioelectrodes should be ideally suited to design implantable bioelectronic devices. Because of its high redox potential and ability to reduce oxygen directly to water, human ceruloplasmin, HCp, the only blue multicopper oxidase present in human plasma, appears to be the ultimate biocatalyst for oxygen biosensors and also biocathodes in biological power sources. In comparison to fungal and plant blue multicopper oxidases, e.g. Myrothecium verrucaria bilirubin oxidase and Rhus vernicifera laccase, respectively, the inflammatory response to HCp in human blood is significantly reduced. Partial purification of HCp allowed to preserve the native conformation of the enzyme and its biocatalytic activity. Therefore, electrochemical studies were carried out with the partially purified enzyme immobilised on nanostructured graphite electrodes at physiological pH and temperature. Amperometric investigations revealed low reductive current densities, i.e. about 1.65 µA cm−2 in oxygenated electrolyte and in the absence of any mediator, demonstrating nevertheless direct electron transfer based O2 bioelectroreduction by HCp for the first time. The reductive current density obtained in the mediated system was about 12 µA cm−2. Even though the inflammatory response of HCp is diminished in human blood, inadequate bioelectrocatalytic performance hinders its use as a cathodic bioelement in a biofuel cell.

Background Adverse drug events from preventable medication errors can result in patient morbidity and mortality, and in cost to the healthcare system. Medication reconciliation can improve communication and reduce medication errors at transitions in care. Objective Evaluate the impact of medication reconciliation and counselling intervention delivered by a pharmacist for medical patients on clinical outcomes 30 days after discharge. Setting Sultan Qaboos University Hospital, Muscat, Oman. Methods A randomized controlled study comparing standard care with an intervention delivered by a pharmacist and comprising medication reconciliation on admission and discharge, a medication review, a bedside medication counselling, and a take-home medication list. Medication discrepancies during hospitalization were identified and reconciled. Clinical outcomes were evaluated by reviewing electronic health records and telephone interviews. Main outcome measures Rates of preventable adverse drug events as primary outcome and healthcare resource utilization as secondary outcome at 30 days post discharge. Results A total of 587 patients were recruited (56 ± 17 years, 57% female); 286 randomized to intervention; 301 in the standard care group. In intervention arm, 74 (26%) patients had at least one discrepancy on admission and 100 (35%) on discharge. Rates of preventable adverse drug events were significantly lower in intervention arm compared to standard care arm (9.1 vs. 16%, p = 0.009). No significant difference was found in healthcare resource use. Conclusion The implementation of an intervention comprising medication reconciliation and counselling by a pharmacist has significantly reduced the rate of preventable ADEs 30 days post discharge, compared to the standard care. The effect of the intervention on healthcare resource use was insignificant. Pharmacists should be included in decentralized, patient-centred roles. The findings should be interpreted in the context of the study's limitations.

Water is a vital component regulating the properties of topical formulations and their interaction with biological barriers, such as skin and mucosa. Changing the watercontent within the frame of the pharmaceutical triangle will have a huge impact on which type of formulation, such as a cream, ointment, gel, or lotion, is formed, as well as the physical properties of the formulation. The composition of a formulation, and the subsequent reformulation after application, will govern the features of the residual film. This will in turn affect the barrier properties of the underlying tissue and consequently the penetration of various substances across skin or mucosa.The primary aim of this thesis has been to provide further understanding on differences between traditional surfactant-based formulations and particle-stabilized, Pickering, formulations and how specific excipients, like alcohols, emollients, and thickeners can affect their physical and/or sensorial properties. The secondary aim has been to gain more knowledge on the role of water in topical formulations and how it affects the properties of the underlaying tissue on application.

By combining a portfolio of physicochemical techniques combined with sensory science, we have been able to identify differences between Pickering and surfactantstabilized formulations. Starch-based Pickering emulsions were perceived as less greasy and sticky than traditional creams, even at high oil content. Moreover, we were able develop a novel type of alcohol-based Pickering emulsion with combined moisturizing and antiseptic properties. We have also been able to link sensory attributes, evaluated by human volunteers, with physicochemical characterizations. Furthermore, the in vitro ForceBoard™ method was developed further and we evaluated its potential to be used as an ex vivo method using excised skin. In addition, we have shown that that the water gradient over a biological barrier has a general relevance with respect to drug absorption and should be considered not only in dermaldrug delivery but also for buccal and nasal drug delivery.

Tactile perception can be investigated through ex vivo friction measurements using a so-called ForceBoard™, providing objective assessments and savings in time and money, compared to a subjective human panel. In this work we aim to compare excised skin versus VitroSkin® as model substrates for tactile friction measurements. A further aim is to detect possible differences between traditional surfactant-based creams, and a particle-stabilized (Pickering) cream and investigate how the different substrates affect the results obtained. It was found that the difference in tactile friction between excised skin and VitroSkin® was small on untreated substrates. When topical creams were applied, the same trends were observed for both substrates, although the frictional variation over time relates to the difference in surface structure between the two substrates. The results also confirmed that there is a difference between starch-based Pickering formulations and surfactant-based creams after application, indicating that the latter is greasier than Pickering cream. It was also shown that the tactile friction of Pickering emulsions was consistently high even with high amounts of oil, indicating a non-greasy, and non-sticky formulation. The characteristics of starch-stabilized Pickering formulations make them promising candidates in the development of surfactant-free topical formulations with unique tactile properties.

Rising consumer demands for safer, more natural, and sustainable topical products have led to increased interest in finding alternative excipients, while retaining functionality and cosmetic appeal. Particle-stabilized Pickering creams have emerged as possible alternatives to replace traditional surfactant-stabilized creams and are thus one of the focuses in this study. The aim of this paper was to study relationships between sensorial characteristics and physical properties to understand how different excipients affect these aspects, comparing one starch particle–stabilized and three surfactant-stabilized formulations. A human panel was used to evaluate sensorial perception, while physical properties were deduced by rheology and tactile friction, together with in vivo and ex vivo skin hydration measurements.

The results show that sensorial attributes related to the application phase can be predicted with rheology, while afterfeel attributes can be predicted with tactile friction studies. Differences in rheological and sensory properties among surfactant-based creams could mainly be attributed to the type of emollients used, presence of thickeners and surfactant composition. Differences between surfactant-based creams and a Pickering cream were more evident in relation to the afterfeel perception. Presence of starch particles in the residual film on skin results in high tactile friction and low perception of residual coating, stickiness, greasiness, and slipperiness in sensorial afterfeel.

Formulations for buccal drug delivery often comprise polymers to facilitate mucoadhesion based on water sorption. The main objective of the current study was therefore to evaluate the effect of dehydration on drug uptake through oral mucosa. We have used diffusion cells with excised porcine mucosa to study uptake of three alternative drugs (i.e., Metronidazole, Benzydamine and Xylometazoline) together with polyethylene glycol (PEG) as the model polymer for adjusting water activity in the test solutions. Taking drug activity into account, we can conclude that addition of PEG results in a drug flux through mucosa that is about two times lower for Metronidazole and more than 40 times lower for Xylometazoline compared to that from a pure PBS-solution. However, for Benzydamine the uptake through mucosa was more or less the same, which could possibly be due to the high PEG-concentration (65 wt%) affecting the dissociation constant and thus the permeability. These results indicate that an increased water gradient may have the same limiting effect on permeability through oral mucosa as previously seen for skin. Thus, water gradient effects should be a factor to consider when developing buccal adhesive formulations.

Formulations for nasal drug delivery often rely on water sorption to adhere to the mucosa, which also causes a higher water gradient over the tissue and subsequent dehydration. The primary aim of this study was therefore to evaluate mucosal response to dehydration and resolve the hypothesis that mucoadhesion achieved through water sorption could also be a constraint for drug absorption via the nasal route. The effect of altering water activity of the vehicle on Xylometazoline HCl and Cr-EDTA uptake was studied separately using flow through diffusion cells and excised porcine mucosa. We have shown that a modest increase in the water gradient over mucosa induces a substantial decrease in drug uptake for both Xylometazoline HCl and Cr-EDTA. A similar result was obtained when comparing two different vehicles on the market; Nasoferm (Nordic Drugs, Sweden) and BLOX4 (Bioglan, Sweden). Mucoadhesion based on water sorption can slow down drug uptake in the nasal cavity. However, a clinical study is required to determine whether prolonged duration of the vehicle or preventing dehydration of the mucosa is the most important factor for improving bioavailability.

Legionella omfattar bakterier som kan orsaka allvarliga lunginflammationer, såsom legionärsjuka hos människor. Den mest kända sjukdomen som kan orsakas av Legionella-bakterier är legionärsjuka, en allvarlig typ av pneumoni, vilket utgörs oftast av Legionella pneumophila (L. pneumophila). L. pneumophila kan delas in i olika serogrupperbaserat på variationer i dess yttre strukturer. Majoriteten av L. pneumophila framkallas av serogrupp 1. I Region Skåne utgörs diagnostiken med PCR analys för att detektera Legionella bakterier, som utförs på nedre luftvägsprov (Bronkoalveolärt lavage, förkortad BAL, och sputum). PCR-positiva prover odlas för att artbestämmas genom riktad odling på fasta substrat för att därefter försöka matcha Legionella stammen som isolerats från miljöer med patienten genom genetisk typning. Då odling av Legionella stammen för artbestämning vanligtvis ej svaras ut förens efter 10 dygn kan utvärdering av en PCR förenkla särskiljning av serogrupp 1 och serogrupp 2-14 för att kunna behandla patienten i tid. Wzm-PCR:en anses kunna särskilja serogrupp 1 från serogrupp 2-14. Genom att odla fram L. pneumophila serogrupp 1 isolat och därefter utföra en spädningsserie kunde PCR-effektiviteten med hjälp utav qPCR beräknas vara 96,5 %. L. pneumophila serogrupp 2-14 odlades även ut för att kunna avskilja serogrupp 1 från serogrupp 2-14 vilket gavs till resultat, men vidare testning av metoden behövs.

Tuberkulos är en luftburen smitta orsakad av Mycobacterium tuberculosis, som främst påverkar lungorna. Multiresistent tuberkulos (MDR – TB) utvecklas till följd av att inadekvat behandling blivit insatt och är svårbehandlad eftersom bakterien utvecklat resistens mot de mest effektiva tuberkulosläkemedlena. Konventionell oral administrering av Klofazimin (KLZ) orsakar ofta biverkningar som hudmissfärgningar, vilket ökar risken för att patienter ska upphöra behandling i förtid. Läkemedelsleverans till lungorna med mesoporösa kiseldioxid partiklar (MSP) är ett alternativt tillvägagångssätt vid behandling av MDR – TB som erbjuder lösningar till tidigare nämnda problem. Syftet med studien var att undersöka hur MSP I, II och III:s olika egenskaper påverkar KLZ:s in vitro löslighet i stimulerad lungvätska. Ytterligare ett syfte var att avgöra om den frisatta läkemedelskoncentrationen överskred den minsta hämmande koncentrationen (MIC) som används vid behandling av MDR – TB. KLZ – laddade MSP:er med en koncentration på 25 mg/l genomgick löslighetsstudier med hjälp av sample and separate (SS) och dialysmembran (DM) metoder. Resultaten visade att KLZ frisatt från MSP I, II och III erhöll liknande maximala koncentrationer och precipitationsmönster med SS metoden. ANOVA visade en statistiskt signifikant högre koncentration av frigjord KLZ från MSP I jämfört med MSP III. Det indikerade att MSP:ernas egenskaper påverkade KLZ löslighet. DM metoden resulterade i icke – kvantifierbara nivåer av KLZ. Svepelektronmikroskop bekräftade MSP:ernas degradering från mikro – till nanostorlek i simulerad lungvätska och det observerades skillnader i partiklarnas degradering till följd av deras olika halveringstider. Dessutom var en koncentration på 25 mg/l otillräcklig för att uppnå KLZ koncentrationer som överskred MIC. Det innebar att KLZ inte hade kunnat hämma tillväxten av de resistenta stammarna av M. tuberculosis.

Vaccin upptäcktes under 1800-talets smittkoppspandemi och har sedan dess blivit ett väsentligt medel för bekämpning av diverse sjukdomar. Ett vaccin behöver en adjuvant som stärker dess effekt, idag är aluminium-baserade adjuvanter (ABA) en av de vanligaste i bruk. ABA har framställts empiriskt utan förståelse för dess mekanism och inverkan på immunförsvaret. Världens forskare är eniga om att ABA via fagocytos tas upp av fagocyterande celler och påverkar immunaktiveringen. Syftet med studien var att undersöka om ABA påverkar makrofagers metabolism så att cellernas huvudsakliga energiutvinning sker via glykolysen alternativt oxidativ fosforylering. Glykolysens slutprodukt är pyruvat, som efter en rad reaktioner omvandlas till laktat. Av den orsak analyserades mängden laktat före och efter inkubation av olika koncentrationer av ABA Alhydrogel. Studien omfattar försök med både en human myeolid cellinje THP-1 och humana perifera monocyter som isolerats från leukocytkoncentrat. Differentiering av cellinjerna till makrofager av M1, M2 och M0 utfördes med hjälp av stimuleringsfaktorn LPS och cytokiner som IL-4, IL-13 och INF-γ. Efter avslutad inkubation med ABA polades cellernas medium för analys av laktatkoncentration. Dessutom analyserades det relativa antalet celler i brunnarna från vilka mediet hade isolerats. Laktat mängden analyserades med Lactate-GloTM Assay och cellviabliteten mättes med CellTiterFluorTM Cell Viability Assay. Resultaten påvisade en märkbar förändring i laktatproduktionen hos cellerna, särskilt hos M1 makrofagerna. Laktatkoncentrationen visade ett maximum vid inkubering av lägre koncentrationer av ABA. Högre koncentrationer av ABA visade däremot en reduktion av laktat och cellers viabilitet. Utifrån erhållna resultat kan det konstateras att fagocytos av ABA påverkar makrofagers metabolism och inducerar en glykolytisk metabolism, men att vid höga koncentrationer leder till celldöd. Dock behövs mer arbete med upprepande experiment för att erhålla statiskt signifikanta resultat.

Dendritiska celler (DC) är en form av fagocyterande och antigenpresenterande celler som spelar en viktig roll i att koppla samman det medfödda och det adaptiva immunförsvaret. DC har som huvudfunktion att fagocytera främmande ämnen och presentera antigener för naiva T-celler. Under de senaste decennierna har APC-celler spelat en viktig roll inom vaccinutvecklingen. Utvecklingen av vacciner har avsevärt avancerat för att sträva efter ökad säkerhet och färre biverkningar. Tidigare studier har visat att makrofager som exponeras för aluminium-adjuvanter och därmed fagocyterar adjuvanten, får ett ökat intracellulärt innehåll av laktat. Laktatkoncentrationen mättes för att utvärdera eventuella förändringar i metabolismen efter fagocytos av Al-adjuvanter. Mätningen av laktatkoncentrationen utfördes med hjälp av Lactate-Glo^TM Assay-kits, som bygger på en luminescensbaserad metod. Syftet med studien var att undersöka den intracellulära koncentrationen av laktat hos in vitro-differentierade monocythärledda dendritiska celler (MoDCs) som inkuberats med och utan lipopolysackarider (LPS). Resultaten för denna studie har uppvisat en möjlig förändringen i laktatproduktion hos icke aktiverade MoDC efter inkubation med två olika koncentrationer av Aluminium-adjuvanter (Al 2,5 µg/ml och Al 5 µg/ml). När det gäller MoDC aktiverade med LPS så påvisade studien ingen signifikant påverkan av aluminium-adjuvanten på cellernas laktatproduktion. För att kunna dra slutsatser krävdes flera ytterligare försök för att fastställa om det förekommer en signifikant påverkning av laktatkoncentrationen hos dendritiska celler efter exponering med hjälp av Al-adjuvant.

Lipid mediators, small molecules involved in regulating inflammation and its resolution, are a class of lipids of wide interest as their levels in blood and tissues may be used to monitor health and disease states or the effect of new treatments. These molecules are present at low levels in biological samples, and an enrichment step is often needed for their detection. We describe a rapid and selective method that uses new low-cost molecularly imprinted (MIP) and non-imprinted (NIP) polymeric sorbents for the extraction of lipid mediators from plasma and tissue samples. The extraction process was carried out in solid-phase extraction (SPE) cartridges, manually packed with the sorbents. After extraction, lipid mediators were quantified by liquid chromatography–tandem mass spectrometry (LC–MSMS). Various parameters affecting the extraction efficiency were evaluated to achieve optimal recovery and to reduce non-specific interactions. Preliminary tests showed that MIPs, designed using the prostaglandin biosynthetic precursor arachidonic acid, could effectively enrich prostaglandins and structurally related molecules. However, for other lipid mediators, MIP and NIP displayed comparable recoveries. Under optimized conditions, the recoveries of synthetic standards ranged from 62% to 100%. This new extraction method was applied to the determination of the lipid mediators concentration in human plasma and mouse tissues and compared to other methods based on commercially available cartridges. In general, the methods showed comparable performances. In terms of structural specificity, our newly synthesized materials accomplished better retention of prostaglandins (PGs), hydroxydocosahexaenoic acid (HDoHE), HEPE, hydroxyeicosatetraenoic acids (HETE), hydroxyeicosatrienoic acid (HETrE), and PUFA compounds, while the commercially available Strata-X showed a higher recovery for dihydroxyeicosatetraenoic acid (diHETrEs). In summary, our results suggest that this new material can be successfully implemented for the extraction of lipid mediators from biological samples. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.

α1-microglobulin (A1M) är en antioxidant som hittas i de flesta vävnader och kan binda heme och andra fria radikaler. A1M kodas av alpha-1-mikroglobulin/bikunin prekursor (AMBP) genen tillsammans med proteas-hämmaren bikunin. Nyligen utvecklades en knock-out (KO)-model med A1M, men hittills har modellen inte använts för att utforska A1Ms roll i perinatal hjärnutveckling. Därför syftar denna studie till att utforska A1Ms roll i perinatal hjärnutveckling, och dess effekt på övergången till en högre syremättad miljö vid födsel, genom att studera genuttryck av oxidativ stress, inflammation, hjärnutveckling, och antioxidant markörer med hjälp av A1M KO-musmodellen. Studiedesignen inkluderade jämförelser mellan vildtyp (WT) och homozygota (HO) möss vid fyra tidpunkter mellan gestationsåldern 19,5 dagar och postnatal dag 10. För att studera genuttryck, extraherades RNA från hjärnvävnad, sedan syntetiserades cDNA som analyserades med qPCR med SYBR Green som detektionsmetod. I studien fann vi att A1M uttrycks i låga nivåer i hjärnan. Ett annat intresse för studien var att undersöka  oxidativ stress relaterad till övergången från en syrefattig miljö i utero till en mer syrerik miljö efter födsel. Vi observerade inga signifikanta genuttrycksförändringar i någon av de studerade generna vid födsel i jämförelse med andra tidpunkter. Det är viktigt att poängtera att en potentiell begränsning i den aktuella studien är de små provgruppstorlekarna (n=5 i 4 tidsgrupper för vardera genotyp). Sammanfattningsvis verkar inte A1M spela en betydande roll i neonatal hjärnutveckling eller i övergången till en mer syrerik miljö under friska förhållanden, men ytterligare studier förespråkas. 

Malignt mesoteliom är en ovanlig och aggressiv cancerform. Den är vanligast i pleura och kallas då pleuralt mesoteliom. Vanligaste orsaken till utveckling av pleuralt mesoteliom är exponering för asbest. Insjuknande patienter har dålig prognos och begränsade behandlingsmöjligheter. Röntgen kan i ett första stadie ge diagnos av sjukdomen. Oftast behövs också en biopsi tas för fastställande av diagnos. Även provtagning av pleuravätskan kan ge värdefull diagnostisk information. Immunhistokemi är en viktig tilläggsanalys för diagnos med biopsier och cellblock. Immunhistokemi innebär färgning med antikroppar. För pleuralt mesoteliom kan exempelvis antikropparna PD-L1 och calretinin användas för diagnostisering. I denna studie färgades 10 parade pleurabiopsier och cellblock från pleuravätska från patienter med malignt mesoteliom. Antikropparna PD-L1 och calretinin användes. Syftet var att utvärdera uttrycket av PD-L1 och calretinin. Alla infärgade glas undersöktes i ljusmikroskop. För PD-L1 ansågs enbart membranfärgning som positivt infärgad och för calretinin ansågs cytoplasmatisk och/eller kärnfärgning som positiv. Procentuellt antal positiva tumörceller undersöktes vid två cutoff värden, ≥1 % och ≥10 %. Procentuellt antal <1 % vid cutoff ≥1 % och procentuellt antal <10 % vid cutoff ≥10 % ansågs negativt. Detta gällde för båda antikropparna. Antal positiva och negativa biopsier samt cellblock och konkordansen redovisades. Overall percentage agreement (OPA), Cohen’s kappa (κ) och konfidensintervall (CI) beräknades också. Resultatet visade på lägre antal positiva färgningar för cellblocken jämfört med biopsierna för båda antikropparna. För konkordansen visades att den vara lika för PD-L1 och calretinin vid cutoff ≥1 % men högre för PD-L1 än för calretinin vid cutoff ≥10 %.

Introduction: There is a need for new therapies that can enhance response rates and broaden the number of cancer indications where immunotherapies provide clinical benefit. CD40 targeting therapies provide an opportunity to meet this need by promoting priming of tumor-specific T cells and reverting the suppressive tumor microenvironment. This is supported by emerging clinical evidence demonstrating the benefits of immunotherapy with CD40 antibodies in combination with standard of care chemotherapy.

Areas covered: This review is focused on the coming wave of next-generation CD40 agonists aiming to improve efficacy and safety, using new approaches and formats beyond monospecific antibodies. Further, the current understanding of the role of different CD40 expressing immune cell populations in the tumor microenvironment is reviewed.

Expert opinion: There are multiple promising next-generation approaches beyond monospecific antibodies targeting CD40 in immuno-oncology. Enhancing efficacy is the most important driver for this development, and approaches that maximize the ability of CD40 to both remodel the tumor microenvironment and boost the anti-tumor T cell response provide great opportunities to benefit cancer patients. Enhanced understanding of the role of different CD40 expressing immune cells in the tumor microenvironment may facilitate more efficient clinical development of these compounds.

Detailed impedance and voltammetric studies of hexameric octaheme nitrite reductase immobilized on carbon-based nanomaterials, specifically nanotubes and nanoparticles, were performed. Well-pronounced bioelectrocatalytic reduction of nitrite on enzyme-modified electrodes was obtained. Analysis of the impedance data indicated the absence of long-lived intermediates involved in the nitrite reduction. Cyclic voltammograms of biomodified electrodes had a bi-sigmoidal shape, which pointed to the presence of two enzyme orientations on carbon supports. The maximum (limiting) catalytic currents were determined and, by applying the correction by the mixed kinetics equation, the Tafel dependences were plotted for each catalytic wave/each enzyme orientation. Finally, two schemes for the rate-limiting processes during bioelectrocatalysis were proposed, viz. for low- and high-potential orientations.

We report on the encapsulation of the antibiotic clofazimine (CLZ) within the pores of mesoporous silica particles having hydrophilic (CBET value of 137) and more hydrophobic (CBET value of 94 after calcination at 600 °C) surfaces. We studied the effect of pH on the released amount of CLZ in aqueous solutions and observed a maximum at pH 4.1 in correlation with the solubility of the drug. Less release of the drug was observed from the more hydrophobic particles which was attributed to a difference in the affinity of the drug to the carrier particles. Fluorescence lifetime imaging microscopy, emission spectra, and fluorescence lifetimes of single drug loaded particles provided detailed understanding and new knowledge of the physical form of the encapsulated drug and the distribution within the particles. The distribution of CLZ within the particles was independent of the surface chemistry of the particles. The confirmation of CLZ molecules as monomers or aggregates was revealed by controlled removal of the drug with solvent. Additionally, the observed optical "halo effect" in the fluorescent images was interpreted in terms of specific quenching of high concentration of molecules. The emission lifetime experiments suggest stronger interaction of CLZ with the more hydrophobic particles, which is relevant to its release. The results reported in this work demonstrate that tuning the hydrophilicity/hydrophobicity of mesoporous silica particles can be used as a tool to control the release without impacting their loading ability.

Equilibrium water sorption in stratum corneum (SC) is considered by treating it as a biocomposite with two main phases, namely, corneocytes and lipids. To validate the rule of mixtures for the individual phase sorption isotherms, a new flexible fitting model is introduced by accounting for characteristic features observed in the variations of the thermodynamic correction factors corresponding to the individual sorption isotherms. The comparison of the model fitting performance with that of the five-parameter Park's model shows a remarkably good ability to fit experimental data for different types of sorption isotherms. The effect of the lipids content on the variance of the composite sorption isotherm of stratum corneum is highlighted. The sensitivity analysis reveals that for the typical water content 20-30 wt%, which corresponds to the SC in a stable condition, the sensitivity of the composite sorption isotherm to the variation of the lipids content on dry basis is predominantly positive and sufficiently small. The good agreement observed between the experimental sorption isotherm for SC and the composite isotherm, which is based on the rule of mixtures for the individual phase sorption isotherms, yields a plausible conclusion (hypothesis) that the corneocytes-lipids mechanical interaction during unconstrained swelling of the SC membrane in the in vitro laboratory experiment is negligible.

Application of artificial neural networks (ANNs) for modeling of vapor-liquid equilibrium in multicomponent mixtures is considered. Two novel ANN-based models are introduced, which can be seen as generalizations of the Wilson model and the NRTL model. A unique feature of the proposed approach is that an ANN approximation for the molar excess Gibbs energy generates approximations for the activity coefficients. A special case of the ternary acetic acid-n-propyl alcohol-water system (at 313.15 K) is used to illustrate the efficiency of the different models, including Wilson's model, Focke's model, and the introduced generalized degree-1 homogeneous neural network model. Also, the latter one-level NN model is compared to the Wilson model on 10 binary systems. The efficiency of the two-level NN model is assessed by a comparison with the NRTL model. (C) 2019 Elsevier B.V. All rights reserved.

A new fitting model for sorption by glassy polymers is suggested based on the Flory–Huggins (FH) equation with a composite formula for the FH interaction parameter, χ, which is applicable if sorption experimental data shows a single-maximum variation of the FH parameter. Namely, a power-like and a linear approximation is assumed for χ(φ1), as a function of solvent volume fraction φ1, before and after the point of its maximum. After determining the maximum point from a direct inspection of the sorption data, the three fitting parameters are evaluated by solving two independent least-square minimization problems. Several sorption studies of biopolymers taken from the literature show that the endset of the glass transition region is correlated with the position of the maximum of the FH interaction parameter. Based on this hypothesis and the Vrentas–Vrentas model for sorption of glassy polymers, a theoretical framework for the glass transition analysis is developed. In particular, the solvent-induced glass transition temperature variation can be estimated from the sorption isotherm as a function of the solvent content corresponding to temperatures above the temperature of sorption.

The so-called minimal model is formulated for describing the enthalpy relaxation and recovery in glass transition. The model is based on the Arrhenius law for the enthalpy relaxation, which uses two-dimensional parameters, namely the activation energy and the so-called pre-factor (relaxation time at relatively high temperature). A numerically effective exact analytical solution is obtained for the case of constant-rate differential scanning calorimetry. The developed model is analyzed according to the logic of the model itself without introducing additional simplifying assumptions of thermodynamic nature. For typical range of the model parameters, the resulting differential equation contains a large parameter, which offers an opportunity for the application of asymptotic and approximate techniques. A number of simple approximations have been provided for some thermodynamic quantities of interest.

Gravimetric sedimentation is known as a relatively simple method of determining density of spherical particles. When the method is applied to water-swollen starch microparticles of about submillimeter sizes, it becomes evident that a careful selection of the experimental setup parameters is needed for producing accurate testing results. The main reason for this is that the mean particle density is very close to the density of water, and therefore, a dynamic model accounting for the so-called Bassett history force should be employed for describing the unsteady accelerating particle settling. A main novelty of this study consists in deriving a priori estimates for the settling time and distance.

Diffusion of water through membranes presents a considerable challenge, as the diffusivity often depends on the local concentration of water. One particular example with strong biological relevance is the stratum corneum (SC) as the primary permeability barrier for the skin. A simple alternative for the constant diffusivity model is provided by the Fujita's two-parameter rational approximation, which captures the experimentally observed fact that the SC diffusion constant for water increases with increasing the water concentration. Based on Fick's law of diffusion, a one-dimensional concentration-dependent diffusion model is developed and applied for the analysis of both the steady-state transepidermal water loss (TEWL) and the non-steady-state so-called skin surface water loss (SSWL) occurred after removal of an occlusion patch from the SC surface. It is shown that some of the age-related changes in the SSWL can be qualitatively explained by the variation of the dimensionless Fujita concentration-dependence parameter.

Background The Lund Integrated MedicinesManagement model offers a systematic approach forindividualising and optimising patient drug treatment.Clinical, economical and humanistic outcomes havebeen shown as well as results from the medicationreconciliation process. There is a need also to describethe medication review process.Objective To describe the frequency and types of drug-relatedproblems (DRPs) identified during medicationreviews and to evaluate the actions of the pharmacistsand the physicians regarding the identified DRPs.Method Structured medication reviews were conductedby a multi-professionalteam on top of standard care for719 patients in two internal medicine wards in a SwedishUniversity Hospital. The medication reviews were studiedretrospectively to classify DRPs and actions taken.Results A total of 573 (80%) of patients had at leastone actual DRP; an average of three DRPs per patientand in total 2164. Wrong drug and adverse drug reactionwere the most common types of DRPs. The most frequentmedication groups involved in DRPs were drugs forthe cardiovascular system and the nervous system andthe most frequent substances were warfarin, digoxin,furosemide and paracetamol. The 10 most commonmedications accounted for 27% of the actual DRPs. Ofthe identified DRPs, a total of 1740 (80%) were actedon. The three most common types of adjustments madewere withdrawal of drug therapy, change of drug therapyand initiation of drug therapy. When the pharmacistsuggested an adjustment, the physician implemented88% (1037/1174) of the recommendations.Conclusion DRPs are common among elderly patientswho are admitted to hospital. Systematic identificationof high-riskmedications and common DRP types enablestargeting of prioritised patients for medication reviews.

År 2019 beräknas 13,4 miljoner barn ha fötts för tidigt. En av de mest förekommande terapierna inom neonatalvården är syrgasbehandling, men att ge höga nivåer av syrgas är inte helt riskfritt. Hyperoxi kan ge upphov till toxicitet som orsakas av för höga syrehaltsnivåer. Det finns hypoteser gällande att hyperoxi kan leda till permanenta neurologiska funktionsnedsättningar. Forskningsfältet är överens om att det finns en kunskapslucka gällande vilka syrenivåer som är mest lämpliga att ge till för tidigt födda barn, och ytterligare studier behövs på hur hyperoxi påverkar den omogna hjärnan. Denna studie är ett randomiserat pilotförsök, som använde en kanin-modell som inducerar bronkopulmonell dysplasi (BPD) hos för tidigt födda kaniner. Detta för att undersöka om hyperoxi har någon histologisk effekt på den omogna, utvecklande kaninhjärnan.

I in vivo-experimentet exponerades kaninungar med olika syrenivåer. Olika histologiska analyser utfördes på vävnadsproverna från kaninhjärnorna. Snitten färgades med Mayers hematoxylin och Eosin (H&E) och en immunhistokemisk (IHC) inmärkning av CD31 utfördes. Histologiska analyser utfördes på snitten, och en digital kvantifiering av antalet blodkärl utfördes. Dessutom testades en IHC-inmärkning av CD31 med uppkonverterade nanopartiklar (UCNPs) för att visualisera blodkärlen i hjärnvävnaden.

Inga histopatologiska fynd relaterade till syrenivåer noterades i någon av experimentgrupperna. Vid analys av H&E-färgade sektioner fann man att blodkärlen i hippocampus hos en av hyperoxigrupperna (95% O₂) hade morfologiska skillnader jämfört med deras kontrollgrupp (21% O₂). IHC-färgning av CD31 bekräftade delvis dessa fynd, medan resultaten från den digitala kvantifieringen var ej konklusiva. Visualiseringen med UCNPs genererade inte tydliga bilder av blodkärlen i hjärnvävnaden. Fortsatt utveckling av metoden behövs. Denna pilotstudie betonar vikten av ytterligare forskning för att fortsätta undersöka sambandet mellan hyperoxi och angiogenes i den omogna hjärnan.

When compared to the general population, socioeconomically disadvantaged communitiesfrequently experience compromised health. Monitoring the divide is challenging since standardizedbiomedical tests are linguistically and culturally inappropriate. The aim of this study was to developand test a unique mobile biomedical testbed based on non-invasive analysis, as well as to explorethe relationships between the objective health measures and subjective health outcomes, asevaluated with the World Health Organization Quality of Life survey. The testbed was evaluated in asocioeconomically disadvantaged neighborhood in Malmö, which has been listed as one of the twelvemost vulnerable districts in Sweden. The study revealed that compared to conventional protocolsthe less intrusive biomedical approach was highly appreciated by the participants. Surprisingly, thecollected biomedical data illustrated that the apparent health of the participants from the ethnicallydiverse low-income neighborhood was comparable to the general Swedish population. Statisticallysignificant correlations between perceived health and biomedical data were disclosed, even thoughthe dependences found were complex, and recognition of the manifest complexity needs to beincluded in further research. Our results validate the potential of non-invasive technologies incombination with advanced statistical analysis, especially when combined with linguistically andculturally appropriate healthcare methodologies, allowing participants to appreciate the significanceof the different parameters to evaluate and monitor aspects of health.

Immobilization of enzymes into different support materials has been widely studied as means to control their activity and stability. Here we will consider lipid liquid crystalline phases as enzyme carriers, as they have been demonstrated to have a high potential in a range of applications such as drug delivery, protein encapsulation or crystallization thanks to the wide range of self-assembly structures they can form, which have cavities of nano-scale dimensions. Furthermore, such structures have also been observed in a range of living organisms. Although, reverse cubic or hexagonal lipid aqueous phase can be used to entrap smaller biomolecules, it is still challenging to encapsulate bioactive macromolecules, such as proteins. Here, we will present a novel lipid system able to form highly swollen sponge phases (L3), with aqueous pores up to 13 nm of diameter. We will show that this structure is preserved even in excess aqueous solution, where they form sponge-like nanoparticles (L3 NPs) in which two enzymes of different sizes, Aspartic protease and beta-galactosidase (34 KDa and 460 KDa, respectively), could be included. To reveal the nature of the interaction between the enzymes and the lipid matrix, we studied the adsorption of both proteins on the lipid layers formed by the L3 NPs. The results will be discussed in terms of the ability of these nanoparticles to encapsulate and release of the proteins in the lipid matrix.

Hypothesis Gelatin is one of the most popular constituents of biodegradable/edible films. Because of its poor mechanical properties, it typically needs to be cross-linked. In this regard, the use of tannic acid has attracted significant interest. Whereas the biodegradability of gelatin is well established, little is known on how different crosslinking methods affect biodegradability. In most cases, the ionic strength at which protein films are grown has a drastic effect on their structure. Thus, it is expected that by controlling the ionic strength during the growth of cross-linked gelatin films it should be possible to tune the access to relevant cleavage sites by proteases and, therefore, their biodegradability. Experiments Gelatin-tannic acid were grown at different ionic strengths by means of the layer-by-layer self-assembly method. The growth of these multilayers and their response to the presence of different proteases were monitored by means of Electrochemical Impedance Spectroscopy and Quartz Crystal Microbalance with Dissipation. Findings Gelatin-tannic acid multilayers grown at low ionic strength exhibited a swollen structure that allowed easy access to their cleavage sites by proteases. Multilayers formed at physiological ionic strength exhibited a compacter structure, which limited their proteolytic degradation.

Compound-specific isotope analysis (CSIA) of polar organic micropollutants in environmental waters requires a processing of large sample volumes to obtain the required analyte masses for analysis by gas chromatography/isotoperatio mass spectrometry (GC/IRMS). However, the accumulation of organic matter of unknown isotopic composition in standard enrichment procedures currently compromises the accurate determination of isotope ratios. We explored the use of molecularly imprinted polymers (MIPs) for selective analyte enrichment for C-13/C-12 and N-15/N-14 ratio measurements by GC/IRMS using 1H-benzotriazole, a typical corrosion inhibitor in dishwashing detergents, as example of a widely detected polar organic micropollutant. We developed procedures for the treatment of >10 L of water samples, in which custom-made MIPs enabled the selective cleanup of enriched analytes in organic solvents obtained through conventional solid-phase extractions. Hydrogen bonding interactions between the triazole moiety of 1H-benzotriazole, and the MIP were responsible for selective interactions through an assessment of interaction enthalpies and N-15 isotope effects. The procedure was applied successfully without causing isotope fractionation to river water samples, as well as in- and effluents of wastewater treatment plants containing mu g/L concentrations of 1H-benzotriazole and dissolved organic carbon (DOC) loads of up to 28 mg C/L. MIP-based treatments offer new perspectives for CSIA of organic micropollutants through the reduction of the DOC-to-micropollutant ratios.

Bulk and magnetic core-shell Molecularly Imprinted Polymers (MMIPs) have been introduced and compared to extract and determine amiodarone from a complex matrix, i.e., plasma, due to the importance of Therapeutic Drug Monitoring (TDM). Polymer synthesis was confirmed by FTIR, AFM, TGA, DLS, VSM, TEM, and the adsorption studies such as capacity, isothermal models, selectivity, and regeneration were performed to evaluate and compare polymer efficiency in extraction and separation of amiodarone from sample solutions and human plasma. Both nano-sized and bulk polymers successfully extracted the target molecule at the low therapeutic ranges and the overdose concentrations (recoveries of 98.38%-102.70%). The maximum adsorption capacity of the MMIPs was 42.5 mu g/mg compared with 2.6 mu g/mg for bulk polymers. The imprinting factors of the polymers were 15.12 and 6.84 for MMIPs and bulk, respectively. MMIPs and bulk polymers presented 4.68 and 1.66 selectivity factors, respectively, towards amiodarone compared with lidocaine. LOD, LOQ and enrichment factor in human plasma were 0.09, 0.28 mu g mL(-1), and 10 respectively. Recoveries of therapeutic concentration from plasma were 91.38 and 97.33% for bulk and MMIPs, respectively. MMIPs as an adsorbent in amiodarone extraction from plasma offered reduced necessary sample amount, less adsorbent consumption, reduced pretreatment time, and reduced elution solvent waste while yielding higher extraction recovery and more specificity for the target compared with the bulk polymer. Bulk polymers have a more straightforward synthesis procedure due to fewer synthesis steps and fewer variables, and Molecularly Imprinted Polymer Solid-phase Extraction (MIP-SPE) has already been introduced commercially. MMIPs prevail on a small scale, and in the context of a simple extraction, separation, or concentration in large-scale bioanalysis, efforts towards optimization and development of MMIPs can unearth tremendous opportunities for green chemistry principles. 

Dicarboxylate metallosurfactants (AASM), synthesized by mixing N-dodecyl aminomalonate, -aspartate and -glutamate with CaCl2, MnCl2 and CdCl2, were characterized by XRD, FTIR, and NMR spectroscopy. Layered structures, formed by metallosurfactants, were evidenced from differential scanning calorimetry and thermogravimetric analyses. Solvent-spread monolayer of AASM in combination with soyphosphatidylcholine (SPC) and cholesterol (CHOL) were studied using Langmuir surface balance. With increasing mole fraction of AASM mean molecular area increased and passed through maxima at ~60 mol% of AASMs, indicating molecular packing reorganization. Systems with 20 and 60 mol% AASM exhibited positive deviations from ideal behavior signifying repulsive interaction between the AASM and SPC, while synergistic interactions were established from the negative deviation at other combinations. Dynamic surface elasticity increased with increasing surface pressure signifying formation of rigid monolayer. Transition of monolayer from gaseous to liquid expanded to liquid condensed state was established by Brewster angle microscopic studies. Stability of the hybrid vesicles, formed by AASM+SPC+CHOL, was established by monitoring their size, zeta potential and polydispersity index values over 100 days. Size and spherical morphology of hybrid vesicles were confirmed by transmission electron microscopic studies. Biocompatibility of the hybrid vesicles were established by cytotoxicity studies revealing their possible applications in drug delivery and imaging.

Soft materials are usually defined as materials made of mesoscopic entities, often self-organised, sensitive to thermal fluctuations and to weak perturbations. Archetypal examples are colloids, polymers, amphiphiles, liquid crystals, foams. The importance of soft materials in everyday commodity products, as well as in technological applications, is enormous, and controlling or improving their properties is the focus of many efforts. From a fundamental perspective, the possibility of manipulating soft material properties, by tuning interactions between constituents and by applying external perturbations, gives rise to an almost unlimited variety in physical properties. Together with the relative ease to observe and characterise them, this renders soft matter systems powerful model systems to investigate statistical physics phenomena, many of them relevant as well to hard condensed matter systems. Understanding the emerging properties from mesoscale constituents still poses enormous challenges, which have stimulated a wealth of new experimental approaches, including the synthesis of new systems with, e.g. tailored self-assembling properties, or novel experimental techniques in imaging, scattering or rheology. Theoretical and numerical methods, and coarse-grained models, have become central to predict physical properties of soft materials, while computational approaches that also use machine learning tools are playing a progressively major role in many investigations. This Roadmap intends to give a broad overview of recent and possible future activities in the field of soft materials, with experts covering various developments and challenges in material synthesis and characterisation, instrumental, simulation and theoretical methods as well as general concepts.

The formation of molecular assemblies in protein solutions is of strong interest both from a fundamental viewpoint and for biomedical applications. While ordered and desired protein assemblies are indispensable for some biological functions, undesired protein condensation can induce serious diseases. As a common cofactor, the presence of salt ions is essential for some biological processes involving proteins, and in aqueous suspensions of proteins can also give rise to complex phase diagrams including homogeneous solutions, large aggregates, and dissolution regimes. Here, we systematically study the cluster formation approaching the phase separation in aqueous solutions of the globular protein BSA as a function of temperature (T), the protein concentration (c(p)) and the concentrations of the trivalent salts YCl3 and LaCl3 (c(s)). As an important complement to structural, i.e. time-averaged, techniques we employ a dynamical technique that can detect clusters even when they are transient on the order of a few nanoseconds. By employing incoherent neutron spectroscopy, we unambiguously determine the short-time self-diffusion of the protein clusters depending on c(p), c(s) and T. We determine the cluster size in terms of effective hydrodynamic radii as manifested by the cluster center-of-mass diffusion coefficients D. For both salts, we find a simple functional form D(c(p), c(s), T) in the parameter range explored. The calculated inter-particle attraction strength, determined from the microscopic and short-time diffusive properties of the samples, increases with salt concentration and temperature in the regime investigated and can be linked to the macroscopic behavior of the samples.

The crowded environment of biological systems such as the interior of living cells is occupied by macromolecules with a broad size distribution. This situation of polydispersity might influence the dependence of the diffusive dynamics of a given tracer macromolecule in a monodisperse solution on its hydrodynamic size and on the volume fraction. The resulting size dependence of diffusive transport crucially influences the function of a living cell. Here, we investigate a simplified model system consisting of two constituents in aqueous solution, namely, of the proteins bovine serum albumin (BSA) and bovine polyclonal gamma-globulin (Ig), systematically depending on the total volume fraction and ratio of these constituents. From high-resolution quasi-elastic neutron spectroscopy, the separate apparent short-time diffusion coefficients for BSA and Ig in the mixture are extracted, which show substantial deviations from the diffusion coefficients measured in monodisperse solutions at the same total volume fraction. These deviations can be modeled quantitatively using results from the short-time rotational and translational diffusion in a two-component hard sphere system with two distinct, effective hydrodynamic radii. Thus, we find that a simple colloid picture well describes short-time diffusion in binary mixtures as a function of the mixing ratio and the total volume fraction. Notably, the self-diffusion of the smaller protein BSA in the mixture is faster than the diffusion in a pure BSA solution, whereas the self-diffusion of Ig in the mixture is slower than in the pure Ig solution.

With continuously improving signal-to-noise ratios, a statistically sound analysis of quasi-elasticneutron scattering (QENS) spectra requires to fit increasingly complex models which poses several challenges.Simultaneous fits of the spectra for all recorded values of the momentum transfer become a standard approach.Spectrometers at spallation sources can have a complicated non-Gaussian resolution function which has to bedescribed most accurately. At the same time, to speed up the fitting, an analytical convolution with this resolutionfunction is of interest. Here, we discuss basic concepts to efficient approaches for fits of QENS spectra basedon standard MATLAB and Python fit algorithms. We illustrate the fits with example data from IN16B, BASIS,and BATS.