Colon cancer is one of the most common types of cancer worldwide, predominantly consisting of carcinomas originating from epithelial tissue. A key mechanism in its development involves mutations in the tumor suppressor gene adenomatous polyposis coli (APC), which plays a crucial role in tumor progression through its interaction with β-catenin in the Wnt signaling pathway. Traditionally research has relied on two-dimensional (2D) cell models, where cells grow as a monolayer on a flat surface. However, these models have significant limitations, as they lack the natural cell-cell interactions and tissue morphology found in vivo. To overcome these, three-dimensional (3D) models such as spheroids or organoids have been developed. These models better mimicthe physiological conditions in the body. In this study a 3D model was established using FN-silk, providing structural support during the growth phase. The silk is similar to the extracellular matrix, which is what cells are surrounded by in the body. The cell lines HCT116 and HT29 were characterized using immunofluorescence and immunohistochemistry. The epithelial markers Ecadherin and EGFR were used to analyze the expression patterns and compare differences between 2D and 3D models. The results revealed distinct expression patterns between the two cell lines. HT29 consistently exhibited stronger Ecadherin expression compared to HCT116, while EGFR expression was more pronounced in the 3D model of HCT116 than in its 2D counterpart. Additionally 3D models displayed more artifacts in IF visualization compared to 2D cultures.Further optimization and validation of the method is needed to fully explore the 3D-models potential.