Exploring the Surface: Sampling of Potential Skin Cancer Biomarkers Kynurenine and Tryptophan, Studied on 3D Melanocyte and Melanoma Models.Visa övriga samt affilieringar
2024 (Engelska)Ingår i: Biomolecules, E-ISSN 2218-273X, Vol. 14, nr 7, artikel-id 815
Artikel i tidskrift (Refereegranskat) Published
Abstract [en]
Early detection of cancer via biomarkers is vital for improving patient survival rates. In the case of skin cancers, low-molecular-weight biomarkers can penetrate the skin barrier, enabling non-invasive sampling at an early stage. This study focuses on detecting tryptophan (Trp) and kynurenine (Kyn) on the surface of reconstructed 3D melanoma and melanocyte models. This is examined in connection with IDO-1 and IL-6 expression in response to IFN-γ or UVB stimulation, both crucial factors of the melanoma tumor microenvironment (TME). Using a polystyrene scaffold, full-thickness human skin equivalents containing fibroblasts, keratinocytes, and melanocytes or melanoma cells were developed. The samples were stimulated with IFN-γ or UVB, and Trp and Kyn secretion was measured using HPLC-PDA and HPLC-MS. The expression of IDO-1 and IL-6 was measured using RT-qPCR. Increased Trp catabolism to Kyn was observed in IFN-γ-stimulated melanoma and melanocyte models, along with higher IDO-1 expression. UVB exposure led to significant changes in Kyn levels but only in the melanoma model. This study demonstrates the potential of skin surface Trp and Kyn monitoring to capture TME metabolic changes. It also lays the groundwork for future in vivo studies, aiding in understanding and monitoring skin cancer progression.
Ort, förlag, år, upplaga, sidor
MDPI, 2024. Vol. 14, nr 7, artikel-id 815
Nyckelord [en]
IDO-1, IFN-γ, UVB, full-thickness 3D skin models, kynurenine, melanoma, non-invasive sampling, skin cancer biomarkers, tryptophan
Nationell ämneskategori
Cancer och onkologi
Identifikatorer
URN: urn:nbn:se:mau:diva-70066DOI: 10.3390/biom14070815ISI: 001278168300001PubMedID: 39062529Scopus ID: 2-s2.0-85199639039OAI: oai:DiVA.org:mau-70066DiVA, id: diva2:1886612
2024-08-022024-08-022024-09-18Bibliografiskt granskad