Publikationer från Malmö universitet
Ändra sökning
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
TOPICAL SAMPLING OF POTENTIAL SKIN CANCER BIOMARKERS, KYNURENINE AND TRYPTOPHAN: STUDY ON A 3D MELANOMA MODEL
2022 (Engelska)Självständigt arbete på avancerad nivå (masterexamen), 20 poäng / 30 hpStudentuppsats (Examensarbete)
Abstract [en]

Background: Malignant melanoma continues to be one of the deadliest forms of skin cancer. Despite immense international efforts, it remains a major clinical challenge. To date, the golden standard for skin cancer diagnosis is through visual inspection and biopsies. UVB (280-320 nm) and IFN-γ have been proven to induce IDO-1 expression in tumor cells, increasing the ratio between tryptophan (Trp) and kynurenine (Kyn) in the tumor microenvironment. Non-invasive sampling of kyn and trp could thereby serve as an alternative for skin cancer diagnostics. 

Objective: This study aimed to investigate the clinical relevance of monitoring trp and kyn by stimulation of skin cancer development in a 3D melanoma model.

Methods: Polystyrene scaffolds were used to create 3D melanoma and melanocyte models respectively. Monolayers were used to examine keratinocytes, fibroblasts, melanoma cells, and melanocytes' response to stimuli. After treatment with UVB and IFN-g, the release of cytokines was measured with ELISA, and gene expression was analyzed with quantitative reverse transcription PCR. Secreted trp and kyn were quantified by high-performance liquid chromatography. The 3D models were then sectioned into 3 µm thick pieces for histological analysis and immunohistochemistry staining.

Results: Significantly increased gene expression of IDO-1 was seen in all monolayers, including the 3D models stimulated with IFN-g. Released IL-6 was induced after UVB exposure in the 3D models, and IL-1a was only detected in the melanocyte model. Both models showed an increase in kyn levels after stimulation with IFN-g. No induced IDO-1 gene expression could be detected in the models after UVB exposure, and no significant change in Trp/Kyn ratio was detected in those samples. 

Conclusion: Our results suggest that IFN-g induces IDO-1 gene expression in 3D models, leading to an increased trp/kyn ratio. Topical sampling of kyn and trp may be possible as an alternative non-invasive method to present diagnostics. UVB seemed to induce inflammation in the 3D models, but no significant signs of malignant transformation.

Ort, förlag, år, upplaga, sidor
2022.
Nyckelord [en]
3D models, IDO-1, Kynurenine, Monolayers, Pro-inflammatory cytokines, Tryptophan.
Nationell ämneskategori
Medicinsk bioteknologi (med inriktning mot cellbiologi (inklusive stamcellsbiologi), molekylärbiologi, mikrobiologi, biokemi eller biofarmaci)
Identifikatorer
URN: urn:nbn:se:mau:diva-54591OAI: oai:DiVA.org:mau-54591DiVA, id: diva2:1690941
Utbildningsprogram
HS Biomedical Surface Science
Handledare
Examinatorer
Tillgänglig från: 2022-09-01 Skapad: 2022-08-28 Senast uppdaterad: 2022-09-01Bibliografiskt granskad

Open Access i DiVA

Fulltext saknas i DiVA

Sök vidare i DiVA

Av författaren/redaktören
Miletic Dahlström, Ruzica
Medicinsk bioteknologi (med inriktning mot cellbiologi (inklusive stamcellsbiologi), molekylärbiologi, mikrobiologi, biokemi eller biofarmaci)

Sök vidare utanför DiVA

GoogleGoogle Scholar

urn-nbn

Altmetricpoäng

urn-nbn
Totalt: 98 träffar
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf