Publikationer från Malmö universitet
Ändra sökning
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
Mechanistic characterization of an oxygen reduction reaction-driven, fully enzymatic and self-calibrating pH biosensor based on wired bilirubin oxidase
Vilnius Univ, Life Sci Ctr, Inst Biochem, Dept Bioanal, Sauletekio Al 7, LT-10257 Vilnius, Lithuania.;Vilnius Gediminas Tech Univ, Dept Chem & Bioengn, Sauletekio Al 11, LT-10223 Vilnius, Lithuania..
Vilnius Univ, Life Sci Ctr, Inst Biochem, Dept Bioanal, Sauletekio Al 7, LT-10257 Vilnius, Lithuania..
Malmö universitet, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV). Malmö universitet, Biofilms Research Center for Biointerfaces.ORCID-id: 0000-0001-6421-2158
Vilnius Univ, Life Sci Ctr, Inst Biochem, Dept Bioanal, Sauletekio Al 7, LT-10257 Vilnius, Lithuania..
Visa övriga samt affilieringar
2022 (Engelska)Ingår i: Sensors and actuators. B, Chemical, ISSN 0925-4005, E-ISSN 1873-3077, Vol. 367, artikel-id 132054Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

The oxygen reduction reaction (ORR) is important due to high electrochemical potential. Multicopper oxidases (e.g., bilirubin oxidase (BOx)) belong to a class of enzymes that catalyze ORR with low overpotential, and have attracted significant attention in designing bioelectronic devices, viz., biosensors and enzymatic cathodes of biofuel cells. In this work, we present a new concept - an enzyme driven pH electrode system composed of two electrodes: a pH-sensitive BOx-based working electrode and pH-insensitive, a BOx-based reference electrode. ORR-driven pH monitoring was conducted by measuring the potential difference between the two electrodes. Moreover, the electrode system was self-supported and self-calibrating, and no additional electrodes (Pt counter or Ag/AgCl reference) were needed for measurements. The BOx-based pH sensor was tested in continuous and real-time pH monitoring with high accuracy. Also, we present an in-depth mechanistic study explaining the electrode potential-pH dependence, which brings new insights into the operational principle of bilirubin oxidase at different pH.

Ort, förlag, år, upplaga, sidor
Elsevier, 2022. Vol. 367, artikel-id 132054
Nyckelord [en]
Bilirubin oxidase, Enzyme electrode, pH electrode, Oxygen reduction reaction, Reference electrode, Self-referencing electrode
Nationell ämneskategori
Analytisk kemi
Identifikatorer
URN: urn:nbn:se:mau:diva-54054DOI: 10.1016/j.snb.2022.132054ISI: 000807773900004Scopus ID: 2-s2.0-85133977564OAI: oai:DiVA.org:mau-54054DiVA, id: diva2:1685200
Tillgänglig från: 2022-08-02 Skapad: 2022-08-02 Senast uppdaterad: 2024-02-05Bibliografiskt granskad

Open Access i DiVA

Fulltext saknas i DiVA

Övriga länkar

Förlagets fulltextScopus

Person

Shleev, Sergey

Sök vidare i DiVA

Av författaren/redaktören
Shleev, SergeyRatautas, Dalius
Av organisationen
Institutionen för biomedicinsk vetenskap (BMV)Biofilms Research Center for Biointerfaces
I samma tidskrift
Sensors and actuators. B, Chemical
Analytisk kemi

Sök vidare utanför DiVA

GoogleGoogle Scholar

doi
urn-nbn

Altmetricpoäng

doi
urn-nbn
Totalt: 128 träffar
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf