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Fluorescent Molecularly Imprinted Polymer Layers against Sialic Acid on Silica-Coated Polystyrene Cores-Assessment of the Binding Behavior to Cancer Cells.
Malmö University, Faculty of Health and Society (HS), Department of Biomedical Science (BMV).
Chemical and Optical Sensing Division, Bundesanstalt für Materialforschung und -prüfung (BAM), Richard-Willstätter Straße 11, 12489 Berlin, Germany.
Malmö University, Faculty of Health and Society (HS), Department of Biomedical Science (BMV). Malmö University, Biofilms Research Center for Biointerfaces.
Institute of Biomedicine, University of Turku, 20520 Turku, Finland; FICAN West Cancer Centre, Turku University Hospital, 20520 Turku, Finland.
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2022 (English)In: Cancers, ISSN 2072-6694, Vol. 14, no 8, article id 1875Article in journal (Refereed) Published
Abstract [en]

Sialic acid (SA) is a monosaccharide usually linked to the terminus of glycan chains on the cell surface. It plays a crucial role in many biological processes, and hypersialylation is a common feature in cancer. Lectins are widely used to analyze the cell surface expression of SA. However, these protein molecules are usually expensive and easily denatured, which calls for the development of alternative glycan-specific receptors and cell imaging technologies. In this study, SA-imprinted fluorescent core-shell molecularly imprinted polymer particles (SA-MIPs) were employed to recognize SA on the cell surface of cancer cell lines. The SA-MIPs improved suspensibility and scattering properties compared with previously used core-shell SA-MIPs. Although SA-imprinting was performed using SA without preference for the α2,3- and α2,6-SA forms, we screened the cancer cell lines analyzed using the lectins Maackia Amurensis Lectin I (MAL I, α2,3-SA) and Sambucus Nigra Lectin (SNA, α2,6-SA). Our results show that the selected cancer cell lines in this study presented a varied binding behavior with the SA-MIPs. The binding pattern of the lectins was also demonstrated. Moreover, two different pentavalent SA conjugates were used to inhibit the binding of the SA-MIPs to breast, skin, and lung cancer cell lines, demonstrating the specificity of the SA-MIPs in both flow cytometry and confocal fluorescence microscopy. We concluded that the synthesized SA-MIPs might be a powerful future tool in the diagnostic analysis of various cancer cells.

Place, publisher, year, edition, pages
MDPI, 2022. Vol. 14, no 8, article id 1875
Keywords [en]
SA conjugates, cancer, imprinting, molecularly imprinted polymers, sialic acid
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:mau:diva-51285DOI: 10.3390/cancers14081875ISI: 000786858400001PubMedID: 35454783Scopus ID: 2-s2.0-85127781436OAI: oai:DiVA.org:mau-51285DiVA, id: diva2:1656017
Available from: 2022-05-04 Created: 2022-05-04 Last updated: 2024-02-05Bibliographically approved
In thesis
1. Connecting sialic acid expression to cancer cell characteristics: Novel tools for detection, imaging, and analysis
Open this publication in new window or tab >>Connecting sialic acid expression to cancer cell characteristics: Novel tools for detection, imaging, and analysis
2022 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Sialic acid (SA) plays a crucial role in many biological processes. Cell surface SA expression is usually analyzed with antibodies or lectins; however, they are costly and with poor stability. We have used a molecular imprinting technique to synthesize an alternative SA receptor – SA molecularly imprinted polymers(SA-MIPs) with an embedded fluorophore for fluorescent detection of theSA-MIPs. The binding behavior and specificity of SA-MIPs were verified by using lectins and SA conjugates on cancer cell lines, showing that SA-MIPs can be used as an effective tool for SA expression analysis of cancer cells. Digital holographic cytometry (DHC) is a non-phototoxic quantitative phase imaging technique that facilitates the monitoring of living cells over time. We have demonstrated the potential of DHC by mapping cellular parameters, such as cell number, area, thickness, and volume. In addition, cellular parameters possibly depending on sialylation, were evaluated using DHC. Furthermore, the uptake over time of SA-MIPs by macrophages was investigated for any inflammatory and/or cytotoxic responses when administered to phagocytosing cells. Our results indicate that SA-MIPs caused low induction and sparse secretion of inflammatory cytokines, and that reduced cell proliferation was not due to cytotoxicity, but to attenuated cell cycles. These results suggest that SA-MIPs will contribute to the further understanding of cancer cell behavior and can be an asset for in vivo studies.

Place, publisher, year, edition, pages
Malmö: Malmö University Press, 2022. p. 83
Series
Malmö University Health and Society Dissertations, ISSN 1653-5383 ; 2022:9
Keywords
Molecularly imprinted polymers, digital holographic cytometry, cancer, cytotoxicity, inflammation, sialic acid
National Category
Medical and Health Sciences
Research subject
Health and society
Identifiers
urn:nbn:se:mau:diva-56210 (URN)10.24834/isbn.9789178773251 (DOI)978-91-7877-326-8 (ISBN)9789178773251 (ISBN)
Public defence
2022-12-15, AS:E002, Malmö, 10:12 (English)
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Note

Incorrect ISBN in publication: 978-91-7877-326-1 (pdf)

Paper II in dissertation as manuscript and is not included in the fulltext online 

Available from: 2022-11-25 Created: 2022-11-25 Last updated: 2024-03-07Bibliographically approved

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Beyer, SarahZhang, YuechengSternbæk, LouisePersson, Jenny L.Ohlsson, LarsEl-Schich, ZahraGjörloff Wingren, AnetteStollenwerk, Maria M

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