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Tailored polymer-based selective extraction of lipid mediators from biological samples
Precision Medicine Translational Research Programme and Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 119077, Singapore; SLING, Singapore Lipidomics Incubator, Life Sciences Institute, National University of Singapore, Singapore 119077, Singapore; Department of Molecular Metabolism, Harvard T.H. Chan School of Public Health, Harvard University, Cambridge, MA 02138, USA.
Department of Chemistry, University of Oslo, 0315 Oslo, Norway; Hormone Laboratory, Department of Medical Biochemistry, Oslo University Hospital, 0424 Oslo, Norway.
Department of Chemistry, University of Oslo, 0315 Oslo, Norway.
Department of Chemistry, University of Oslo, 0315 Oslo, Norway.
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2021 (Engelska)Ingår i: Metabolites, ISSN 2218-1989, E-ISSN 2218-1989, Vol. 11, nr 8, artikel-id 539Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Lipid mediators, small molecules involved in regulating inflammation and its resolution, are a class of lipids of wide interest as their levels in blood and tissues may be used to monitor health and disease states or the effect of new treatments. These molecules are present at low levels in biological samples, and an enrichment step is often needed for their detection. We describe a rapid and selective method that uses new low-cost molecularly imprinted (MIP) and non-imprinted (NIP) polymeric sorbents for the extraction of lipid mediators from plasma and tissue samples. The extraction process was carried out in solid-phase extraction (SPE) cartridges, manually packed with the sorbents. After extraction, lipid mediators were quantified by liquid chromatography–tandem mass spectrometry (LC–MSMS). Various parameters affecting the extraction efficiency were evaluated to achieve optimal recovery and to reduce non-specific interactions. Preliminary tests showed that MIPs, designed using the prostaglandin biosynthetic precursor arachidonic acid, could effectively enrich prostaglandins and structurally related molecules. However, for other lipid mediators, MIP and NIP displayed comparable recoveries. Under optimized conditions, the recoveries of synthetic standards ranged from 62% to 100%. This new extraction method was applied to the determination of the lipid mediators concentration in human plasma and mouse tissues and compared to other methods based on commercially available cartridges. In general, the methods showed comparable performances. In terms of structural specificity, our newly synthesized materials accomplished better retention of prostaglandins (PGs), hydroxydocosahexaenoic acid (HDoHE), HEPE, hydroxyeicosatetraenoic acids (HETE), hydroxyeicosatrienoic acid (HETrE), and PUFA compounds, while the commercially available Strata-X showed a higher recovery for dihydroxyeicosatetraenoic acid (diHETrEs). In summary, our results suggest that this new material can be successfully implemented for the extraction of lipid mediators from biological samples. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.

Ort, förlag, år, upplaga, sidor
MDPI, 2021. Vol. 11, nr 8, artikel-id 539
Nyckelord [en]
Lipid mediators, Molecularly imprinted polymer (MIP), Non-imprinted polymer (NIP), Solid-phase extraction (SPE), Strata-X
Nationell ämneskategori
Analytisk kemi
Identifikatorer
URN: urn:nbn:se:mau:diva-45067DOI: 10.3390/metabo11080539ISI: 000689440200001PubMedID: 34436480Scopus ID: 2-s2.0-85112699897OAI: oai:DiVA.org:mau-45067DiVA, id: diva2:1586869
Tillgänglig från: 2021-08-23 Skapad: 2021-08-23 Senast uppdaterad: 2023-10-03Bibliografiskt granskad

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Shinde, SudhirkumarSellergren, Börje

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Shinde, SudhirkumarSellergren, Börje
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Institutionen för biomedicinsk vetenskap (BMV)Biofilms Research Center for Biointerfaces
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