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Franz cells for facile biosensor evaluation: A case of HRP/SWCNT-based hydrogen peroxide detection via amperometric and wireless modes
Malmö universitet, Biofilms Research Center for Biointerfaces. Malmö universitet, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV). The University of Sydney, Australia.
Malmö universitet, Biofilms Research Center for Biointerfaces. Malmö universitet, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV).
Malmö universitet, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV). Malmö universitet, Biofilms Research Center for Biointerfaces.
The University of Sydney, Australia.
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2021 (Engelska)Ingår i: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 191, artikel-id 113420Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Reducing animal use in biosensor research requires broader use of in vitro methods. In this work, we present a novel application of Franz cells suitable for biosensor development and evaluation in vitro. The work describes how Franz cell can be equipped with electrodes enabling characterization of biosensors in close proximity to skin. As an example of a sensor, hydrogen peroxide biosensor was prepared based on horseradish peroxidase (HRP)/single-walled carbon nanotube (SWCNT)-modified textile. The electrode exhibited lower detection limit of 0.3 μM and sensitivity of 184 μA mM−1 cm−2. The ability of this biosensor to monitor H2O2 penetration through skin and dialysis membranes was evaluated in Franz cell setup in amperometric and wireless modes. The results also show that catalase activity present in skin is a considerable problem for epidermal sensing of H2O2. This work highlights opportunities and obstacles that can be addressed by assessment of biosensors in Franz cell setup before progressing to their testing in animals and humans.

Ort, förlag, år, upplaga, sidor
Elsevier, 2021. Vol. 191, artikel-id 113420
Nyckelord [en]
Epidermal sensing, Franz cell, Hydrogen peroxide biosensor, Skin membrane, Animals, Biosensors, Electrodes, Oxidation, Amperometric, H$-2$/O$-2$, Horse-radish peroxidase, Hydrogen peroxide biosensors, Hydrogen peroxide detections, In-vitro, Single-walled carbon, Hydrogen peroxide
Nationell ämneskategori
Analytisk kemi
Identifikatorer
URN: urn:nbn:se:mau:diva-44657DOI: 10.1016/j.bios.2021.113420ISI: 000685993400006PubMedID: 34182432Scopus ID: 2-s2.0-85108599982OAI: oai:DiVA.org:mau-44657DiVA, id: diva2:1578818
Tillgänglig från: 2021-07-07 Skapad: 2021-07-07 Senast uppdaterad: 2023-10-31Bibliografiskt granskad
Ingår i avhandling
1. Development of Wireless Biosensors Integrated into the Radio Frequency Antenna for Chipless and Battery-less Monitoring of Biological Reactions
Öppna denna publikation i ny flik eller fönster >>Development of Wireless Biosensors Integrated into the Radio Frequency Antenna for Chipless and Battery-less Monitoring of Biological Reactions
2023 (Engelska)Doktorsavhandling, sammanläggning (Övrigt vetenskapligt)
Abstract [en]

Development of wireless sensors and biosensors is currently experiencing a rapid progress with a substantial focus directed toward highlighting their potential applications as non-invasive wearables, implants, and highly mobile point-of-care devices. Integration of wireless biosensors into the Internet of Things (IoT) is widely acknowledged as a technological advancement with the potential to significantly change daily life. To maximize this potential, simple integration of biosensors with wireless communication elements would be advantageous. In this regard, systems functioning in chipless, and battery-less modes outperform integrated circuit (IC) based and battery-powered wireless biosensors. Nevertheless, the accessibility of these wireless designs is still limited. In this study, we present a novel approach where incorporating silver nanoparticles(AgNPs) as a part of the radio frequency (RF) tag antenna enables the realization of simple, chipless, and battery-less wireless sensing of biological oxidation and reduction reactions. We exemplified the mechanism of operation in such systems by electronic wiring of enzymes through direct electron transfer (DET) and microorganisms through mediated electron transfer (MET) to the redox conversion of Ag/AgCl. The wiring was designed to facilitate the transformation of metallic AgNPs into AgCl (Ag → AgCl) or the conversion of AgCl particles back into metallic AgNPs (AgCl → Ag) when the enzymatic/microorganism based electron transfer reactions were present. These reactions occurring on the biosensor RF tag antenna strongly changed the impedance of the tag, which was wirelessly monitored by a radio frequency identification (RFID) reader. The functionality of the proposed setup in direct electron transfer coupling of the enzymatic reactions to the redox conversion of the Ag/AgCl was demonstrated by wireless detection of glucose in whole blood samples and hydrogen peroxide penetrated through the skin membrane using the enzymes glucose dehydrogenase(GDH) and horseradish peroxidase (HRP). Additionally, the capability of the proposed configuration in mediated electron transfer wiring of microorganisms to the Ag/AgCl electrochemistry was shown by wireless monitoring of medically relevant microbial biofilms in simulated wound fluid. Generalizing, the results of this work, for the first time, demonstrated that exploiting Ag/AgCl as a part of the tag antenna allows simple, chipless, and battery-less wireless sensing of biological oxidation and reduction reactions.

Ort, förlag, år, upplaga, sidor
Malmö: Malmö University Press, 2023. s. 108
Serie
Malmö University Health and Society Dissertations, ISSN 1653-5383 ; 99
Nationell ämneskategori
Kemi Teknik och teknologier Medicinsk bioteknologi
Identifikatorer
urn:nbn:se:mau:diva-63289 (URN)10.24834/isbn.9789178774128 (DOI)9789178774111 (ISBN)9789178774128 (ISBN)
Disputation
2023-10-27, AS: E002, Faculty of Health and Society, 09:15
Opponent
Handledare
Anmärkning

Paper IV in dissertation as manuscript.

Tillgänglig från: 2023-10-31 Skapad: 2023-10-30 Senast uppdaterad: 2024-02-27Bibliografiskt granskad

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Shafaat, AtefehJankovskaja, SkaidreRuzgas, Tautgirdas

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