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Autotolerant ceruloplasmin based biocathodes for implanted biological power sources
Malmö University, Faculty of Health and Society (HS), Department of Biomedical Science (BMV).
Russia Saint-Petersburg State University, Russia.
University of Seville, Spain.
Malmö University, Faculty of Health and Society (HS), Department of Biomedical Science (BMV).ORCID iD: 0000-0001-9847-5132
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2021 (English)In: Bioelectrochemistry, ISSN 1567-5394, E-ISSN 1878-562X, Vol. 140Article in journal (Refereed) Published
Abstract [en]

High-performance autotolerant bioelectrodes should be ideally suited to design implantable bioelectronic devices. Because of its high redox potential and ability to reduce oxygen directly to water, human ceruloplasmin, HCp, the only blue multicopper oxidase present in human plasma, appears to be the ultimate biocatalyst for oxygen biosensors and also biocathodes in biological power sources. In comparison to fungal and plant blue multicopper oxidases, e.g. Myrothecium verrucaria bilirubin oxidase and Rhus vernicifera laccase, respectively, the inflammatory response to HCp in human blood is significantly reduced. Partial purification of HCp allowed to preserve the native conformation of the enzyme and its biocatalytic activity. Therefore, electrochemical studies were carried out with the partially purified enzyme immobilised on nanostructured graphite electrodes at physiological pH and temperature. Amperometric investigations revealed low reductive current densities, i.e. about 1.65 µA cm−2 in oxygenated electrolyte and in the absence of any mediator, demonstrating nevertheless direct electron transfer based O2 bioelectroreduction by HCp for the first time. The reductive current density obtained in the mediated system was about 12 µA cm−2. Even though the inflammatory response of HCp is diminished in human blood, inadequate bioelectrocatalytic performance hinders its use as a cathodic bioelement in a biofuel cell.

Place, publisher, year, edition, pages
Elsevier, 2021. Vol. 140
Keywords [en]
Biological fuel cells; Blood; Electrolytes; Electron transport properties; Enzyme activity; Enzyme electrodes; Oxygen; pH; Purification; Redox reactions, Biocatalytic activity; Bioelectronic device; Direct electron transfer; Electrochemical studies; Inflammatory response; Nanostructured graphite; Native conformation; Partial purification, Graphite electrodes
National Category
Analytical Chemistry
Identifiers
URN: urn:nbn:se:mau:diva-42684DOI: 10.1016/j.bioelechem.2021.107794ISI: 000663599600011PubMedID: 33744681Scopus ID: 2-s2.0-85102974379OAI: oai:DiVA.org:mau-42684DiVA, id: diva2:1560110
Available from: 2021-06-03 Created: 2021-06-03 Last updated: 2023-11-29Bibliographically approved

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Aleksejeva, OlgaGustafsson, AnnaEriksson, HåkanLjunggren, LennartShleev, Sergey

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