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Paper-Based Competitive Immunochromatography Coupled with an Enzyme-Modified Electrode to Enable the Wireless Monitoring and Electrochemical Sensing of Cotinine in Urine
Biosensors and Bioanalytical Technology for Cells and Innovative Testing Device Research Unit, Chulalongkorn University, Bangkok 10330, Thailand.
Biosensors and Bioanalytical Technology for Cells and Innovative Testing Device Research Unit, Chulalongkorn University, Bangkok 10330, Thailand.
Malmö universitet, Biofilms Research Center for Biointerfaces. Malmö universitet, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV).ORCID-id: 0000-0003-0304-7528
Biosensors and Bioanalytical Technology for Cells and Innovative Testing Device Research Unit, Chulalongkorn University, Bangkok 10330, Thailand; Department of Clinical Chemistry, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok 10330, Thailand.
2021 (Engelska)Ingår i: Sensors, E-ISSN 1424-8220, Vol. 21, nr 5, artikel-id 1659Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

This paper proposes a combined strategy of using paper-based competitive immunochromatography and a near field communication (NFC) tag for wireless cotinine determination. The glucose oxidase labeled cotinine antibody specifically binds free cotinine in a sample, whereas the unoccupied antibody attached to BSA-cotinine at the test line on a lateral flow strip. The glucose oxidase on the strip and an assistant pad in the presence of glucose generated H2O2 and imposed the Ag oxidation on the modified electrode. This enabled monitoring of immunoreaction by either electrochemical measurement or wireless detection. Wireless sensing was realized for cotinine in the range of 100–1000 ng/mL (R2 = 0.96) in PBS medium. Undiluted urine samples from non-smokers exhibited an Ag-oxidation rate three times higher than the smoker’s urine samples. For 1:8 diluted urine samples (smokers), the proposed paper-based competitive immunochromatography coupled with an enzyme-modified electrode differentiated positive and negative samples and exhibited cotinine discrimination at levels higher than 12 ng/mL. This novel sensing platform can potentially be combined with a smartphone as a reader unit.

Ort, förlag, år, upplaga, sidor
MDPI, 2021. Vol. 21, nr 5, artikel-id 1659
Nyckelord [en]
cotinine, immunochromatography, nanomaterials, wireless biosensor
Nationell ämneskategori
Analytisk kemi
Identifikatorer
URN: urn:nbn:se:mau:diva-41182DOI: 10.3390/s21051659ISI: 000628546900001PubMedID: 33670868Scopus ID: 2-s2.0-85101721543OAI: oai:DiVA.org:mau-41182DiVA, id: diva2:1536350
Tillgänglig från: 2021-03-10 Skapad: 2021-03-10 Senast uppdaterad: 2023-10-11Bibliografiskt granskad

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