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Thin poly(vinyl alcohol) cryogels: reactive groups, macropores and translucency in microtiter plate assays
Malmö University, Faculty of Health and Society (HS), Department of Biomedical Science (BMV).
2019 (English)In: Heliyon, E-ISSN 2405-8440, Vol. 5, no 11, article id e02913Article in journal (Refereed) Published
Abstract [en]

Thin macroporous poly(vinyl alcohol) (PVA) hydrogels were produced by cross-linking of PVA in a semi-frozen state with glutaraldehyde (GA) on glass slides or in the wells of microtiter plates. The 100-130 mu m-thick gels were mechanically transferable, squamous translucent films with a high porosity of 7.2 +/- 0.3 mL/g dry PVA i.e. similar to larger cylindrical PVA monoliths of the same composition. Additional treatment of the gels with 1% GA increased the aldehyde group content from 0.7 to 2.4 mu mol/mL as estimated using dinitrophenylhydrazine (DNPH) reagent. Translucency of the gels allowed registration of UV-visible spectra of the DNP' ;Wined films. The catalytic activity of trypsin covalently immobilized on thin gels in the microtiter plates was estimated with chromogenic substrate directly in the wells, and indicated that the amount of protein immobilized was at least 0.34 mg/mL gel. Human immunoglobulin G (IgG) immobilized on thin gels at 0.1-10 mg/mL starting concentrations could be detected in a concentration-dependent manner due to recognition by anti-human rabbit IgG conjugated with peroxidase and photometric registration of the enzymatic activity. The results indicate good permeability of the hydrogel pores for macromolecular biospecific reagents and suggest applications of thin reactive PVA hydrogels in photometric analytical techniques.

Place, publisher, year, edition, pages
Elsevier, 2019. Vol. 5, no 11, article id e02913
Keywords [en]
Chemistry, Chemical engineering, Materials science, Multiwell, Aldehyde, Film, Photometry, Hydrogel
National Category
Polymer Chemistry
Identifiers
URN: urn:nbn:se:mau:diva-39171DOI: 10.1016/j.heliyon.2019.e02913ISI: 000500530100154PubMedID: 31844765Scopus ID: 2-s2.0-85075502604OAI: oai:DiVA.org:mau-39171DiVA, id: diva2:1518111
Available from: 2021-01-15 Created: 2021-01-15 Last updated: 2024-02-05Bibliographically approved

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Ljunggren, Lennart

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