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Peptide discs as precursors of biologically relevant supported lipid bilayers
Niels Bohr Institute, University of Copenhagen, Universitetsparken 5, 2100 Copenhagen, Denmark.
Malmö universitet, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV). Malmö universitet, Biofilms Research Center for Biointerfaces.ORCID-id: 0000-0002-7405-6125
Niels Bohr Institute, University of Copenhagen, Universitetsparken 5, 2100 Copenhagen, Denmark.
Institut Laue Langevin, 71 avenue des Martyrs, 38000 Grenoble, France.
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2021 (Engelska)Ingår i: Journal of Colloid and Interface Science, ISSN 0021-9797, E-ISSN 1095-7103, Vol. 585, s. 376-385, artikel-id S0021-9797(20)31605-2Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Supported lipid bilayers (SLBs) are commonly used to investigate the structure and dynamics of biological membranes. Vesicle fusion is a widely exploited method to produce SLBs. However, this process becomes less favoured when the vesicles contain complex lipid mixtures, e.g. natural lipid extracts. In these cases, it is often necessary to change experimental parameters, such as temperature, to unphysiological values to trigger the SLB formation. This may induce lipid degradation and is also not compatible with including membrane proteins or other biomolecules into the bilayers. Here, we show that the peptide discs, ~10 nm discoidal lipid bilayers stabilized in solution by a self-assembled 18A peptide belt, can be used as precursors for SLBs. The characterizations by means of neutron reflectometry and attenuated total reflectance-FTIR spectroscopy show that SLBs were successfully formed both from synthetic lipid mixtures (surface coverage 90-95%) and from natural lipid mixtures (surface coverage ~85%). Traces of 18A peptide (below 0.02 M ratio) left at the support surface after the bilayer formation do not affect the SLB structure. Altogether, we demonstrate that peptide disc formation of SLBs is much faster than the SLB formation by vesicle fusion and without the need of altering any experimental variable from physiologically relevant values.

Ort, förlag, år, upplaga, sidor
Elsevier, 2021. Vol. 585, s. 376-385, artikel-id S0021-9797(20)31605-2
Nyckelord [en]
ATR-FTIR, Neutron reflectometry, Peptide discs, Supported lipid bilayers
Nationell ämneskategori
Biofysik
Identifikatorer
URN: urn:nbn:se:mau:diva-37777DOI: 10.1016/j.jcis.2020.11.086ISI: 000604569200010PubMedID: 33307306Scopus ID: 2-s2.0-85097582761OAI: oai:DiVA.org:mau-37777DiVA, id: diva2:1514337
Tillgänglig från: 2021-01-05 Skapad: 2021-01-05 Senast uppdaterad: 2023-10-24Bibliografiskt granskad

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Sebastiani, FedericaCárdenas, Marité

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Institutionen för biomedicinsk vetenskap (BMV)Biofilms Research Center for Biointerfaces
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Journal of Colloid and Interface Science
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