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Unstimulated Parotid Saliva Sampling in Juvenile Idiopathic Arthritis and Healthy Controls: A Proof-of-Concept Study on Biomarkers
Malmö universitet, Odontologiska fakulteten (OD). Center For Oral Rehabilitation, Torkelbergsgatan 11, 581 85 Linköping, Department of Biomedical and Clinical Science, Linköping University; postal code 581 83 Linköping, Sweden.ORCID-id: 0000-0002-2260-8442
Pain and Rehabilitation Centre, and Department of Health, Medicine and Caring Sciences, 58183 Linköping, Sweden.ORCID-id: 0000-0002-6396-5104
Center For Oral Rehabilitation, Torkelbergsgatan 11, 581 85 Linköping, Department of Biomedical and Clinical Science, Linköping University; postal code 581 83 Linköping, Sweden.
Malmö universitet, Odontologiska fakulteten (OD). Orofacial Pain and Jaw Function, Institute of Dental Medicine, Karolinska Institute, Alfred Nobels allé 8, 141 52 Huddinge, Sweden; Specialized Pain Rehabilitation, Skåne University Hospital, Lasarettsgatan 13, 222 41 Lund, Sweden.ORCID-id: 0000-0002-8539-7742
2020 (engelsk)Inngår i: Diagnostics (Basel), ISSN 2075-4418, Vol. 10, nr 4, artikkel-id 251Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

The aims of this proof-of-concept study were to develop a collecting method for unstimulated parotid saliva in juvenile idiopathic arthritis (JIA) patients and healthy children and to investigate if inflammatory biomarkers could be detected in these samples. Forty-five children with JIA (median age of 12 years and 25th-75th percentile of 10-15 years; 33 girls and 12 boys) and 16 healthy children as controls (median age of 13 years and 25-75th percentile of 10-13 years; 11 girls and 5 boys) were enrolled in this study. Unstimulated parotid saliva was collected with a modified Carlson-Crittenden collector. The salivary flow rate and salivary concentrations of total protein and inflammatory mediators were assessed. The Meso Scale Discovery electrochemiluminescence immunoassay was used for analyzing protein concentrations and the inflammatory biomarkers. Sufficient parotid saliva volumes to be analyzed could be collected with the collection device. JIA patients had a lower sampling saliva volume (p = 0.008) and saliva flow rate (p = 0.039) than controls. The total protein concentrations and inflammatory biomarkers were measured in the last six healthy subjects. The median protein concentration was 1312 mu g/mL (25th percentile: 844 mu g/mL and 75th percentile: 2062 mu g/mL; n = 6) and quantifiable concentrations of 39 inflammatory proteins could be assessed in these samples. In conclusion, this study indicates that the saliva sampling method, as used in the present study, is able to collect sufficient sample volumes in children, and that it is possible to analyze various inflammatory biomarkers in the collected saliva.

sted, utgiver, år, opplag, sider
MDPI, 2020. Vol. 10, nr 4, artikkel-id 251
Emneord [en]
biomarkers, children, juvenile idiopathic arthritis, parotid saliva, saliva collection
HSV kategori
Identifikatorer
URN: urn:nbn:se:mau:diva-17517DOI: 10.3390/diagnostics10040251ISI: 000534251000042PubMedID: 32344523Scopus ID: 2-s2.0-85083789706OAI: oai:DiVA.org:mau-17517DiVA, id: diva2:1443257
Tilgjengelig fra: 2020-06-18 Laget: 2020-06-18 Sist oppdatert: 2024-06-17bibliografisk kontrollert

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Dimitrijevic Carlsson, AlexandraAlstergren, Per

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