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Strategies for a Direct Characterization of Phosphoproteins on Hydroxyapatite Surfaces
Malmö högskola, Faculty of Health and Society (HS), Department of Biomedical Science (BMV).ORCID iD: 0000-0001-6937-3057
Malmö högskola, Faculty of Health and Society (HS), Department of Biomedical Science (BMV).ORCID iD: 0000-0002-1489-4098
Malmö högskola, Faculty of Odontology (OD).
Malmö högskola, Faculty of Health and Society (HS), Department of Biomedical Science (BMV).
2014 (English)In: Caries Research, ISSN 0008-6568, E-ISSN 1421-976X, Vol. 48, no 2, p. 98-110Article in journal (Refereed) Published
Abstract [en]

We show in this work how systems formed by phosphoproteins on calcium phosphate surfaces can be directly characterized, in real time, in liquid medium, without the need for elution or labeling. Specifically, we show how this is possible by applying three different techniques: ellipsometry, quartz crystal microbalance with dissipation, and atomic force microscopy-based friction force spectroscopy. We apply these techniques to study two different model systems, i.e. those formed upon the adsorption of two model phosphoproteins (κ- and β-casein) on hydroxyapatite (HA) surfaces. Information on the kinetics of adsorption, surface excess, viscoelasticity, water content, thickness of the layers, and protein-surface interaction is provided. Results indicate that both phosphoproteins form homogeneous elastic highly hydrated monolayers on the HA surfaces, the strength of β-casein layers being higher by approximately a factor of 4. Based on the experimental results, models for the conformation of κ- and β-casein molecules adsorbed on HA surfaces are proposed.

Place, publisher, year, edition, pages
S. Karger, 2014. Vol. 48, no 2, p. 98-110
Keywords [en]
Phosphoprotein, casein, hydroxyapatite, atomic force microscopy, friction force spectroscopy, ellipsometry, quartz crystal microbalance with dissipation
National Category
Natural Sciences
Identifiers
URN: urn:nbn:se:mau:diva-14828DOI: 10.1159/000351871ISI: 000331773600004PubMedID: 24296726Scopus ID: 2-s2.0-84897808409Local ID: 16248OAI: oai:DiVA.org:mau-14828DiVA, id: diva2:1418349
Available from: 2020-03-30 Created: 2020-03-30 Last updated: 2024-02-05Bibliographically approved

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Sotres, JavierBarrantes, AlejandroLindh, LiselottArnebrant, Thomas

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