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Adsorption behaviour of statherin and a statherin peptide onto hydroxyapatite and silica surfaces studied by in situ ellipsometry
Malmö högskola, Faculty of Health and Society (HS).
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2008 (English)In: Journal of Colloid and Interface Science, ISSN 0021-9797, E-ISSN 1095-7103, Vol. 318, no 2, p. 175-182Article in journal (Refereed) Published
Abstract [en]

The salivary protein statherin is known to adsorb selectively onto hydroxyapatite (HA), which constitutes the main mineral of the tooth enamel. This adsorption is believed to be crucial for its function as an inhibitor of primary (spontaneous) and secondary (crystal growth) precipitation of calcium phosphate salts present in saliva. A fragment corresponding to the first 21 N-terminus amino acids of statherin (StN21) was previously found to reduce the rate of demineralization of HA. Therefore, the interfacial properties of this peptide and statherin onto silica, hydrophobized silica and HA discs was studied by in situ ellipsometry. Their reversibility induced by dilution and elutability induced by buffer and sodium dodecyl sulfate (SDS) was also determined. The results revealed that statherin adsorbed at a greater extent onto the HA as compared to StN21, suggesting that the hydrogen bonding between the uncharged polar residues at the C-terminal region of statherin and HA contributes to its adsorption. However, on both silica surfaces the peptide adsorption appeared to proceed in a similar way. Onto the hydrophobized silica the adsorption of both peptides was suggested to occur either via multilayer formation or adsorption of aggregates from solution, while onto the hydrophilic silica adsorption of peptide aggregates from solution was the suggested mechanism. Further, both peptides were observed to be strongly adsorbed onto HA, even after SDS treatment, in comparison to the layers adsorbed onto hydrophobized silica. Both peptide layers were found to be weakly adsorbed onto the hydrophilic silica surface as they were totally removed by buffer dilution.

Place, publisher, year, edition, pages
2008. Vol. 318, no 2, p. 175-182
Keywords [en]
Ellipsometry, Statherin, Hydroxyapatite, Silica, Elution, Surfactants
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:mau:diva-4822DOI: 10.1016/j.jcis.2007.11.015ISI: 000252482800005PubMedID: 18054952Scopus ID: 2-s2.0-37349049931Local ID: 7472OAI: oai:DiVA.org:mau-4822DiVA, id: diva2:1401656
Available from: 2020-02-28 Created: 2020-02-28 Last updated: 2024-02-05Bibliographically approved

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Lindh, Liselott

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Faculty of Health and Society (HS)Faculty of Odontology (OD)
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