Malmö University Publications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Monoclonal antibody capture from cell culture supernatants using epitope imprinted macroporous membranes
Lehrstuhl für Technische Chemie II, Universität Duisburg-Essen, Essen, Germany.
Faculty of Chemistry, Technical University of Dortmund, Germany.
Bibitec GmbH, Bielefeld, Germany.
Bibitec GmbH, Bielefeld, Germany.
Show others and affiliations
2016 (English)In: RSC Advances, E-ISSN 2046-2069, Vol. 6, no 58, p. 53162-53169Article in journal (Refereed)
Abstract [en]

Epitope-imprinted membranes targeting the C-terminal fragment of the immunoglobuline G (IgG) heavy chain was developed and used for the purification of a commercial monoclonal antibody. The membranes exhibited strongly enhanced IgG affinity when compared with non-imprinted or IgG imprinted membranes reflected in binding selectivities in a protein mixture (IgG/HSA 1 : 10 w/w) of up to 40, and the elution of 95 to 100% pure IgG after washing. The dynamic binding capacity amounted to 3.9 mg mL(-1) membrane volume with minor loss in performance upon repeated cleaning with alkali. The depletion of host cell proteins from a cell culture broth after production of anti-IL8 antibody using the best performing imprinted membrane under low-salt conditions reached 88% (0.7-1.2 log units) implying an effective removal of impurities from the cell culture supernatant.

Place, publisher, year, edition, pages
Royal Society of Chemistry, 2016. Vol. 6, no 58, p. 53162-53169
Keywords [en]
Chemistry, Multidisciplinary
National Category
Natural Sciences
Identifiers
URN: urn:nbn:se:mau:diva-4578DOI: 10.1039/c6ra06632aISI: 000378563200080Scopus ID: 2-s2.0-84973365179Local ID: 23063OAI: oai:DiVA.org:mau-4578DiVA, id: diva2:1401410
Available from: 2020-02-28 Created: 2020-02-28 Last updated: 2024-06-17Bibliographically approved

Open Access in DiVA

fulltext(980 kB)245 downloads
File information
File name FULLTEXT01.pdfFile size 980 kBChecksum SHA-512
faf77b80ae848c8998bb5f13a067703cc1fd6df9be41116be3c05a562da7ed8414010334cab2eeef5baa3034d4104e2d5650a63a15467d349d848ab64bf5ff89
Type fulltextMimetype application/pdf

Other links

Publisher's full textScopus

Authority records

Sellergren, Börje

Search in DiVA

By author/editor
Sellergren, Börje
By organisation
Department of Biomedical Science (BMV)
In the same journal
RSC Advances
Natural Sciences

Search outside of DiVA

GoogleGoogle Scholar
Total: 245 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
urn-nbn

Altmetric score

doi
urn-nbn
Total: 36 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf