The adsorption of human whole saliva (HWS) onto silica and hydroxyapatite surfaces (HA) was followed by quartz crystal microbalance with dissipation (QCM-D) and ellipsometry. The influence of different surface properties and adsorption media (water and PBS) on the HWS adsorption was studied. The viscoelastic properties of the saliva films formed onto the solid surfaces were estimated by the use of the Voigt-based viscoelastic film model. Furthermore, the efficiency of SDS and delmopinol to elute the adsorbed saliva film from the surfaces was investigated at different surfactant concentrations. It was observed a biphasic kinetic regime for the adsorption of saliva on the silica and HA surfaces, indicating the formation of a rigidly coupled first layer corresponding to an initial adsorption of small proteins and a more loosely bound second layer. The results further showed a higher adsorption of HWS onto the HA surfaces compared to the silica surfaces in both adsorption media (PBS and water). The adsorption in PBS led to higher adsorbed amounts on both surfaces as compared to water. SDS was found to be more efficient in removing the saliva film from both surfaces than delmopinol. The salivary film was found to be less tightly bound on to the silica surfaces since more saliva film could be removed with both SDS and delmopinol compared to from the HA surface. When saliva was adsorbed from PBS a higher energy dissipation was determined implying that a softer saliva layer is build up in PBS as opposed to in water. In the same media (water) a softer saliva layer was formed onto the HA surfaces, while in PBS the saliva layer formed at both surfaces seems to have similar structure.