Publikationer från Malmö universitet
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
Development of Wireless Biosensors Integrated into the Radio Frequency Antenna for Chipless and Battery-less Monitoring of Biological Reactions
Malmö universitet, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV). Malmö universitet, Biofilms Research Center for Biointerfaces.
2023 (Engelska)Doktorsavhandling, sammanläggning (Övrigt vetenskapligt)
Abstract [en]

Development of wireless sensors and biosensors is currently experiencing a rapid progress with a substantial focus directed toward highlighting their potential applications as non-invasive wearables, implants, and highly mobile point-of-care devices. Integration of wireless biosensors into the Internet of Things (IoT) is widely acknowledged as a technological advancement with the potential to significantly change daily life. To maximize this potential, simple integration of biosensors with wireless communication elements would be advantageous. In this regard, systems functioning in chipless, and battery-less modes outperform integrated circuit (IC) based and battery-powered wireless biosensors. Nevertheless, the accessibility of these wireless designs is still limited. In this study, we present a novel approach where incorporating silver nanoparticles(AgNPs) as a part of the radio frequency (RF) tag antenna enables the realization of simple, chipless, and battery-less wireless sensing of biological oxidation and reduction reactions. We exemplified the mechanism of operation in such systems by electronic wiring of enzymes through direct electron transfer (DET) and microorganisms through mediated electron transfer (MET) to the redox conversion of Ag/AgCl. The wiring was designed to facilitate the transformation of metallic AgNPs into AgCl (Ag → AgCl) or the conversion of AgCl particles back into metallic AgNPs (AgCl → Ag) when the enzymatic/microorganism based electron transfer reactions were present. These reactions occurring on the biosensor RF tag antenna strongly changed the impedance of the tag, which was wirelessly monitored by a radio frequency identification (RFID) reader. The functionality of the proposed setup in direct electron transfer coupling of the enzymatic reactions to the redox conversion of the Ag/AgCl was demonstrated by wireless detection of glucose in whole blood samples and hydrogen peroxide penetrated through the skin membrane using the enzymes glucose dehydrogenase(GDH) and horseradish peroxidase (HRP). Additionally, the capability of the proposed configuration in mediated electron transfer wiring of microorganisms to the Ag/AgCl electrochemistry was shown by wireless monitoring of medically relevant microbial biofilms in simulated wound fluid. Generalizing, the results of this work, for the first time, demonstrated that exploiting Ag/AgCl as a part of the tag antenna allows simple, chipless, and battery-less wireless sensing of biological oxidation and reduction reactions.

Ort, förlag, år, upplaga, sidor
Malmö: Malmö University Press, 2023. , s. 108
Serie
Malmö University Health and Society Dissertations, ISSN 1653-5383 ; 99
Nationell ämneskategori
Kemi Teknik och teknologier Medicinsk bioteknologi
Identifikatorer
URN: urn:nbn:se:mau:diva-63289DOI: 10.24834/isbn.9789178774128ISBN: 9789178774111 (tryckt)ISBN: 9789178774128 (digital)OAI: oai:DiVA.org:mau-63289DiVA, id: diva2:1808301
Disputation
2023-10-27, AS: E002, Faculty of Health and Society, 09:15
Opponent
Handledare
Anmärkning

Paper IV in dissertation as manuscript.

Tillgänglig från: 2023-10-31 Skapad: 2023-10-30 Senast uppdaterad: 2024-02-27Bibliografiskt granskad
Delarbeten
1. Glucose-to-Resistor Transduction Integrated into a Radio-Frequency Antenna for Chip-less and Battery-less Wireless Sensing
Öppna denna publikation i ny flik eller fönster >>Glucose-to-Resistor Transduction Integrated into a Radio-Frequency Antenna for Chip-less and Battery-less Wireless Sensing
Visa övriga...
2022 (Engelska)Ingår i: ACS Sensors, E-ISSN 2379-3694, Vol. 7, nr 4, s. 1222-1234Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

To maximize the potential of 5G infrastructure in healthcare, simple integration of biosensors with wireless tag antennas would be beneficial. This work introduces novel glucose-to-resistor transduction, which enables simple, wireless biosensor design. The biosensor was realized on a near-field communication tag antenna, where a sensing bioanode generated electrical current and electroreduced a nonconducting antenna material into an excellent conductor. For this, a part of the antenna was replaced by a Ag nanoparticle layer oxidized to high-resistance AgCl. The bioanode was based on Au nanoparticle-wired glucose dehydrogenase (GDH). The exposure of the cathode-bioanode to glucose solution resulted in GDH-catalyzed oxidation of glucose at the bioanode with a concomitant reduction of AgCl to highly conducting Ag on the cathode. The AgCl-to-Ag conversion strongly affected the impedance of the antenna circuit, allowing wireless detection of glucose. Mimicking the final application, the proposed wireless biosensor was ultimately evaluated through the measurement of glucose in whole blood, showing good agreement with the values obtained with a commercially available glucometer. This work, for the first time, demonstrates that making a part of the antenna from the AgCl layer allows achieving simple, chip-less, and battery-less wireless sensing of enzyme-catalyzed reduction reaction. 

Ort, förlag, år, upplaga, sidor
American Chemical Society (ACS), 2022
Nyckelord
Internet of Things, wireless detection of glucose, direct electron transfer, glucose dehydrogenase, chip-less wireless sensing
Nationell ämneskategori
Analytisk kemi
Identifikatorer
urn:nbn:se:mau:diva-51019 (URN)10.1021/acssensors.2c00394 (DOI)000794994500032 ()35392657 (PubMedID)2-s2.0-85128799436 (Scopus ID)
Forskningsfinansiär
Vetenskapsrådet, 2018-04320KK-stiftelsen, 20170058KK-stiftelsen, 20190010
Tillgänglig från: 2022-04-08 Skapad: 2022-04-08 Senast uppdaterad: 2024-03-05Bibliografiskt granskad
2. A Rapidly Responsive Sensor for Wireless Detection of Early and Mature Microbial Biofilms.
Öppna denna publikation i ny flik eller fönster >>A Rapidly Responsive Sensor for Wireless Detection of Early and Mature Microbial Biofilms.
Visa övriga...
2023 (Engelska)Ingår i: Angewandte Chemie International Edition, ISSN 1433-7851, E-ISSN 1521-3773, Vol. 62, nr 40, artikel-id e202308181Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Biofilm-associated infections, which are able to resist antibiotics, pose a significant challenge in clinical treatments. Such infections have been linked to various medical conditions, including chronic wounds and implant-associated infections, making them a major public-health concern. Early-detection of biofilm formation offers significant advantages in mitigating adverse effects caused by biofilms. In this work, we aim to explore the feasibility of employing a novel wireless sensor for tracking both early-stage and matured-biofilms formed by the medically relevant bacteria Staphylococcus aureus and Pseudomonas aeruginosa. The sensor utilizes electrochemical reduction of an AgCl layer bridging two silver legs made by inkjet-printing, forming a part of near-field-communication tag antenna. The antenna is interfaced with a carbon cloth designed to promote the growth of microorganisms, thereby serving as an electron source for reduction of the resistive AgCl into a highly-conductive Ag bridge. The AgCl-Ag transformation significantly alters the impedance of the antenna, facilitating wireless identification of an endpoint caused by microbial growth. To the best of our knowledge, this study for the first time presents the evidence showcasing that electrons released through the actions of bacteria can be harnessed to convert AgCl to Ag, thus enabling the wireless, battery-less, and chip-less early-detection of biofilm formation.

Ort, förlag, år, upplaga, sidor
John Wiley & Sons, 2023
Nyckelord
Microbial biofilm, chip-less wireless sensing, inkjet printing, mediated electron transfer, near field communication
Nationell ämneskategori
Mikrobiologi
Identifikatorer
urn:nbn:se:mau:diva-62039 (URN)10.1002/anie.202308181 (DOI)001090146000021 ()37490019 (PubMedID)2-s2.0-85168699269 (Scopus ID)
Tillgänglig från: 2023-08-22 Skapad: 2023-08-22 Senast uppdaterad: 2024-04-17Bibliografiskt granskad
3. Franz cells for facile biosensor evaluation: A case of HRP/SWCNT-based hydrogen peroxide detection via amperometric and wireless modes
Öppna denna publikation i ny flik eller fönster >>Franz cells for facile biosensor evaluation: A case of HRP/SWCNT-based hydrogen peroxide detection via amperometric and wireless modes
Visa övriga...
2021 (Engelska)Ingår i: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 191, artikel-id 113420Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Reducing animal use in biosensor research requires broader use of in vitro methods. In this work, we present a novel application of Franz cells suitable for biosensor development and evaluation in vitro. The work describes how Franz cell can be equipped with electrodes enabling characterization of biosensors in close proximity to skin. As an example of a sensor, hydrogen peroxide biosensor was prepared based on horseradish peroxidase (HRP)/single-walled carbon nanotube (SWCNT)-modified textile. The electrode exhibited lower detection limit of 0.3 μM and sensitivity of 184 μA mM−1 cm−2. The ability of this biosensor to monitor H2O2 penetration through skin and dialysis membranes was evaluated in Franz cell setup in amperometric and wireless modes. The results also show that catalase activity present in skin is a considerable problem for epidermal sensing of H2O2. This work highlights opportunities and obstacles that can be addressed by assessment of biosensors in Franz cell setup before progressing to their testing in animals and humans.

Ort, förlag, år, upplaga, sidor
Elsevier, 2021
Nyckelord
Epidermal sensing, Franz cell, Hydrogen peroxide biosensor, Skin membrane, Animals, Biosensors, Electrodes, Oxidation, Amperometric, H$-2$/O$-2$, Horse-radish peroxidase, Hydrogen peroxide biosensors, Hydrogen peroxide detections, In-vitro, Single-walled carbon, Hydrogen peroxide
Nationell ämneskategori
Analytisk kemi
Identifikatorer
urn:nbn:se:mau:diva-44657 (URN)10.1016/j.bios.2021.113420 (DOI)000685993400006 ()34182432 (PubMedID)2-s2.0-85108599982 (Scopus ID)
Tillgänglig från: 2021-07-07 Skapad: 2021-07-07 Senast uppdaterad: 2023-10-31Bibliografiskt granskad
4. Electrogenicity of microbial biofilms of medically relevant microorganisms: potentiometric, amperometric and wireless detection.
Öppna denna publikation i ny flik eller fönster >>Electrogenicity of microbial biofilms of medically relevant microorganisms: potentiometric, amperometric and wireless detection.
Visa övriga...
2024 (Engelska)Ingår i: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 246, artikel-id 115892Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Since the progression of biofilm formation is related to the success of infection treatment, detecting microbial biofilms is of great interest. Biofilms of Gram-positive Staphylococcus aureus and Streptococcus gordonii bacteria, Gram-negative Pseudomonas aeruginosa and Escherichia coli bacteria, and Candida albicans yeast were examined using potentiometric, amperometric, and wireless readout modes in this study. As a biofilm formed, the open circuit potential (OCP) of biofilm hosting electrode (bioanode) became increasingly negative. Depending on the microorganism, the OCP ranged from −70 to −250 mV. The co-culture generated the most negative OCP (−300 mV vs Ag/AgCl), while the single-species biofilm formed by E. coli developed the least negative (−70 mV). The OCP of a fungal biofilm formed by C. albicans was −100 mV. The difference in electrode currents generated by biofilms was more pronounced. The current density of the S. aureus biofilm was 0.9‧10−7 A cm−2, while the value of the P. aeruginosa biofilm was 1.3‧10−6 A cm−2. Importantly, a biofilm formed by a co-culture of S. aureus and P. aeruginosa had a slightly higher negative OCP value and current density than the most electrogenic P. aeruginosa single-species biofilm. We present evidence that bacteria can share redox mediators found in multi-species biofilms. This synergy, enabling higher current and OCP values of multi-species biofilm hosting electrodes, could be beneficial for electrochemical detection of infectious biofilms in clinics. We demonstrate that the electrogenic biofilm can provide basis to construct novel wireless, chip-free, and battery-free biofilm detection method.

Ort, förlag, år, upplaga, sidor
Elsevier, 2024
Nyckelord
Biofilm detection, Microbial biosensor, Open circuit potential, Wireless biosensor
Nationell ämneskategori
Mikrobiologi
Identifikatorer
urn:nbn:se:mau:diva-64686 (URN)10.1016/j.bios.2023.115892 (DOI)001135565500001 ()38056343 (PubMedID)2-s2.0-85178667875 (Scopus ID)
Tillgänglig från: 2023-12-21 Skapad: 2023-12-21 Senast uppdaterad: 2024-02-05Bibliografiskt granskad

Open Access i DiVA

fulltext(16962 kB)411 nedladdningar
Filinformation
Filnamn FULLTEXT01.pdfFilstorlek 16962 kBChecksumma SHA-512
2d9dd1338b2e2a354ba7bbe7e299b7593af04e18d09ab727794a2f3ab68e48fc2395a411db368723063dd08d7cb5c3238a36cfc9f7cbda7030faa598c85aa27b
Typ fulltextMimetyp application/pdf

Övriga länkar

Förlagets fulltext

Person

Shafaat, Atefeh

Sök vidare i DiVA

Av författaren/redaktören
Shafaat, Atefeh
Av organisationen
Institutionen för biomedicinsk vetenskap (BMV)Biofilms Research Center for Biointerfaces
KemiTeknik och teknologierMedicinsk bioteknologi

Sök vidare utanför DiVA

GoogleGoogle Scholar
Totalt: 412 nedladdningar
Antalet nedladdningar är summan av nedladdningar för alla fulltexter. Det kan inkludera t.ex tidigare versioner som nu inte längre är tillgängliga.

doi
isbn
urn-nbn

Altmetricpoäng

doi
isbn
urn-nbn
Totalt: 748 träffar
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf