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Psotta, Carolin
Publications (6 of 6) Show all publications
Psotta, C., Nilsson, E. J., Sjöberg, T. & Falk, M. (2023). Bacteria-Infected Artificial Urine Characterization Based on a Combined Approach Using an Electronic Tongue Complemented with 1H-NMR and Flow Cytometry. Biosensors, 13(10), 916-916
Open this publication in new window or tab >>Bacteria-Infected Artificial Urine Characterization Based on a Combined Approach Using an Electronic Tongue Complemented with 1H-NMR and Flow Cytometry
2023 (English)In: Biosensors, E-ISSN 2079-6374, Vol. 13, no 10, p. 916-916Article in journal (Refereed) Published
Abstract [en]

The prevailing form of bacterial infection is within the urinary tract, encompassing a wide array of bacteria that harness the urinary metabolome for their growth. Through their metabolic actions, the chemical composition of the growth medium undergoes modifications as the bacteria metabolize urine compounds, leading to the subsequent release of metabolites. These changes can indirectly indicate the existence and proliferation of bacterial organisms. Here, we investigate the use of an electronic tongue, a powerful analytical instrument based on a combination of non-selective chemical sensors with a partial specificity for data gathering combined with principal component analysis, to distinguish between infected and non-infected artificial urine samples. Three prevalent bacteria found in urinary tract infections were investigated, Escherichia coli, Klebsiella pneumoniae, and Enterococcus faecalis. Furthermore, the electronic tongue analysis was supplemented with 1H NMR spectroscopy and flow cytometry. Bacteria-specific changes in compound consumption allowed for a qualitative differentiation between artificial urine medium and bacterial growth.

Place, publisher, year, edition, pages
MDPI, 2023
Keywords
electronic tongue, bacterial detection, artificial urine, urinary tract infection, 1H-NMR, flow cytometry
National Category
Microbiology
Identifiers
urn:nbn:se:mau:diva-63142 (URN)10.3390/bios13100916 (DOI)001096540000001 ()37887109 (PubMedID)2-s2.0-85175044453 (Scopus ID)
Available from: 2023-10-13 Created: 2023-10-13 Last updated: 2024-04-11Bibliographically approved
Psotta, C., Cirovic, S., Gudmundsson, P., Falk, M., Mandal, T., Reichhart, T., . . . Shleev, S. (2023). Continuous ex vivo glucose sensing in human physiological fluids using an enzymatic sensor in a vein replica. Bioelectrochemistry, 152, Article ID 108441.
Open this publication in new window or tab >>Continuous ex vivo glucose sensing in human physiological fluids using an enzymatic sensor in a vein replica
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2023 (English)In: Bioelectrochemistry, ISSN 1567-5394, E-ISSN 1878-562X, Vol. 152, article id 108441Article in journal (Refereed) Published
Abstract [en]

Managing blood glucose can affect important clinical outcomes during the intraoperative phase of surgery. However, currently available instruments for glucose monitoring during surgery are few and not optimized for the specific application. Here we report an attempt to exploit an enzymatic sensor in a vein replica that could continuously monitor glucose level in an authentic human bloodstream. First, detailed investigations of the superficial venous systems of volunteers were carried out using ocular and palpating examinations, as well as advanced ultrasound measurements. Second, a tubular glucose-sensitive biosensor mimicking a venous system was designed and tested. Almost ideal linear dependence of current output on glucose concentration in phosphate buffer saline was obtained in the range 2.2-22.0 mM, whereas the dependence in human plasma was less linear. Finally, the developed biosensor was investigated in whole blood under homeostatic conditions. A specific correlation was found between the current output and glucose concentration at the initial stage of the biodevice operation. However, with time, blood coagulation during measurements negatively affected the performance of the biodevice. When the experimental results were remodeled to predict the response without the influence of blood coagulation, the sensor output closely followed the blood glucose level.

Place, publisher, year, edition, pages
Elsevier, 2023
Keywords
Continuous glucose sensing, Enzymatic sensor, Vein replica, Human physiological fluids, Surgery
National Category
Endocrinology and Diabetes
Identifiers
urn:nbn:se:mau:diva-61052 (URN)10.1016/j.bioelechem.2023.108441 (DOI)000984583000001 ()37087795 (PubMedID)2-s2.0-85153044643 (Scopus ID)
Available from: 2023-06-20 Created: 2023-06-20 Last updated: 2024-02-26Bibliographically approved
Psotta, C. (2023). Electrochemical (bio-)sensors operating in human physiological fluids. (Doctoral dissertation). Malmö: Malmö university Press
Open this publication in new window or tab >>Electrochemical (bio-)sensors operating in human physiological fluids
2023 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

This thesis is focused on developing electrochemical (bio-)sensors specifically designed to detect biomolecules and bacteria in human physiological fluids. A more comprehensive understanding of their performance can be obtained by exposing the sensors to real human physiological fluids. Thus, four biosensors were designed and tested in saliva, plasma, blood, and urine. Specifically, a voltammetric electronic tongue, integrating six different electrode materials, was developed to qualitatively assess SARS-CoV-2 in saliva samples using principal component analysis. A tubular enzyme-based sensor utilizing incorporated cellobiose dehydrogenase in an Os(bpy)PVI redox polymer was employed for continuous glucose sensing in human plasma and undiluted whole blood under homeostatic conditions. Two different sensing concepts were developed for the label-free detection of bacteria (Escherichia coli, Enterococcus faecalis, and Klebsiella pneumoniae) in artificial urine and human urine based on metabolic activity due to bacterial growth. The first sensor enabled continuous bacterial detection by reducing Prussian Blue deposited on screen-printed electrodes with wireless data transfer. The second bacterial-sensitive sensor utilized electrochemical characterization to identify three bacteria types based on artificial urine metabolic changes. For a qualitative investigation of the metabolic changes, nuclear magnetic resonance was utilized, and flow cytometry was used to quantify and correlate bacterial growth with electrochemistry. Multivariate statistical data analysis was applied to distinguish bacteria-free and bacteria-infected artificial urine. Finally, an overview of the recent advances in the field of non-invasive electrochemical biosensors operating in secreted human physiological fluids, viz., tears, sweat, saliva, and urine, was give

Abstract [sv]

Denna avhandling är inriktad på utveckling av elektrokemiska (bio-)sensorer som är särskilt utformade för detektering av biomolekyler och bakterier i mänskliga fysiologiska vätskor. Genom att undersöka sensorerna i verkliga mänskliga fysiologiska vätskor, kan man få en mer omfattande förståelse för deras prestanda. Fyra olika biosensorer har utformats och testats i saliv, plasma, blod och urin. En voltammetrisk elektronisk tunga, som integrerar sex olika elektrodmaterial, utvecklades för kvalitativ bedömning av SARS-CoV-2 i salivprover med hjälp av principalkomponentanalys. En rörformad enzymbaserad sensor som använder inkorporerat Cellobiose Dehydrogenase i en Os(bpy)PVI redoxpolymer användes för kontinuerlig glukosmätning i humant plasma och outspätt helblod under homeostatiska förhållanden. Dessutom utvecklades två olika sensorkoncept för märkningsfri detektion av bakterier (Escherichia coli, Enterococcus faecalis och Klebsiella pneumoniae) i artificiell urin och humanurin baserat på metabolisk aktivitet till följd av bakterietillväxt. Den första sensorn möjliggjorde kontinuerlig bakteriedetektion genom reduktion av preussiskt blått elektroder med trådlös dataöverföring. Den andra bakteriekänsliga sensorn använde elektrokemisk karakterisering för att identifiera tre typer av bakterier baserat på metaboliska förändringar i artificiell urin. För en kvalitativ undersökning av de metaboliska förändringarna användes dessutom nukleär magnetisk resonans och flödescytometri för att kvantifiera och korrelera bakterietillväxt med elektrokemi. Multivariat statistisk dataanalys användes för att skilja mellan bakteriefri och bakterieinfekterad (artificiell) urin. Slutligen gavs en översikt över de senaste framstegen inom området för icke-invasiva elektrokemiska biosensorer som arbetar i utsöndrade mänskliga fysiologiska vätskor, dvs. tårar, svett, saliv och urin.

Place, publisher, year, edition, pages
Malmö: Malmö university Press, 2023. p. 74
Series
Malmö University Health and Society Dissertations, ISSN 1653-5383 ; 2023:3
Keywords
biosensor, bacteria, enzyme, sensor, physiological fluid
National Category
Natural Sciences
Identifiers
urn:nbn:se:mau:diva-63045 (URN)10.24834/isbn.9789178773992 (DOI)978-91-7877-398-5 (ISBN)978-91-7877-399-2 (ISBN)
Public defence
2023-09-22, Aulan, Jan Waldenströms gata 25, 14:00 (English)
Opponent
Supervisors
Note

Paper V in dissertation as Manuscript with title "Electrochemical characterization of bacteria-infected artificial urine using an electronic tongue approach"

Available from: 2023-10-10 Created: 2023-10-09 Last updated: 2023-10-13Bibliographically approved
Falk, M., Psotta, C., Cirovic, S., Ohlsson, L. & Shleev, S. (2023). Electronic Tongue for Direct Assessment of SARS-CoV-2-Free and Infected Human Saliva-A Feasibility Study. Biosensors, 13(7), Article ID 717.
Open this publication in new window or tab >>Electronic Tongue for Direct Assessment of SARS-CoV-2-Free and Infected Human Saliva-A Feasibility Study
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2023 (English)In: Biosensors, ISSN 2079-6374, Vol. 13, no 7, article id 717Article in journal (Refereed) Published
Abstract [en]

An electronic tongue is a powerful analytical instrument based on an array of non-selective chemical sensors with a partial specificity for data gathering and advanced pattern recognition methods for data analysis. Connecting electronic tongues with electrochemical techniques for data collection has led to various applications, mostly within sensing for food quality and environmental monitoring, but also in biomedical research for the analyses of different bioanalytes in human physiological fluids. In this paper, an electronic tongue consisting of six electrodes (viz., gold, platinum, palladium, titanium, iridium, and glassy carbon) was designed and tested in authentic (undiluted, unpretreated) human saliva samples from eight volunteers, collected before and during the COVID-19 pandemic. Investigations of 11 samples using differential pulse voltammetry and a principal component analysis allowed us to distinguish between SARS-CoV-2-free and infected authentic human saliva. This work, as a proof-of-principle demonstration, provides a new perspective for the use of electronic tongues in the field of enzyme-free electrochemical biosensing, highlighting their potential for future applications in non-invasive biomedical analyses.

Place, publisher, year, edition, pages
MDPI, 2023
Keywords
electronic tongue, differential pulse voltammetry, principial component analysis, authentic human saliva, SARS-CoV-2
National Category
Analytical Chemistry
Identifiers
urn:nbn:se:mau:diva-61908 (URN)10.3390/bios13070717 (DOI)001038044400001 ()37504115 (PubMedID)2-s2.0-85165896609 (Scopus ID)
Available from: 2023-08-16 Created: 2023-08-16 Last updated: 2024-02-26Bibliographically approved
Psotta, C., Chaturvedi, V., Gonzalez-Martinez, J. F., Sotres, J. & Falk, M. (2023). Portable Prussian Blue-Based Sensor for Bacterial Detection in Urine. Sensors, 23(1), Article ID 388.
Open this publication in new window or tab >>Portable Prussian Blue-Based Sensor for Bacterial Detection in Urine
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2023 (English)In: Sensors, E-ISSN 1424-8220, Vol. 23, no 1, article id 388Article in journal (Refereed) Published
Abstract [en]

Bacterial infections can affect the skin, lungs, blood, and brain, and are among the leading causes of mortality globally. Early infection detection is critical in diagnosis and treatment but is a time- and work-consuming process taking several days, creating a hitherto unmet need to develop simple, rapid, and accurate methods for bacterial detection at the point of care. The most frequent type of bacterial infection is infection of the urinary tract. Here, we present a wireless-enabled, portable, potentiometric sensor for E. coli. E. coli was chosen as a model bacterium since it is the most common cause of urinary tract infections. The sensing principle is based on reduction of Prussian blue by the metabolic activity of the bacteria, detected by monitoring the potential of the sensor, transferring the sensor signal via Bluetooth, and recording the output on a laptop or a mobile phone. In sensing of bacteria in an artificial urine medium, E. coli was detected in similar to 4 h (237 +/- 19 min; n = 4) and in less than 0.5 h (21 +/- 7 min, n = 3) using initial E. coli concentrations of similar to 10(3) and 10(5) cells mL(-1), respectively, which is under or on the limit for classification of a urinary tract infection. Detection of E. coli was also demonstrated in authentic urine samples with bacteria concentration as low as 10(4) cells mL(-1), with a similar response recorded between urine samples collected from different volunteers as well as from morning and afternoon urine samples.

Place, publisher, year, edition, pages
MDPI, 2023
Keywords
portable sensing, bacterial detection, Prussian blue, urine analysis
National Category
Analytical Chemistry
Identifiers
urn:nbn:se:mau:diva-58385 (URN)10.3390/s23010388 (DOI)000908806900001 ()36616986 (PubMedID)2-s2.0-85145976536 (Scopus ID)
Available from: 2023-02-27 Created: 2023-02-27 Last updated: 2024-02-05Bibliographically approved
Falk, M., Psotta, C., Cirovic, S. & Shleev, S. (2020). Non-Invasive Electrochemical Biosensors Operating in Human Physiological Fluids. Sensors, 20(21), 1-28, Article ID 6352.
Open this publication in new window or tab >>Non-Invasive Electrochemical Biosensors Operating in Human Physiological Fluids
2020 (English)In: Sensors, E-ISSN 1424-8220, Vol. 20, no 21, p. 1-28, article id 6352Article, review/survey (Refereed) Published
Abstract [en]

Non-invasive healthcare technologies are an important part of research and development nowadays due to the low cost and convenience offered to both healthcare receivers and providers. This work overviews the recent advances in the field of non-invasive electrochemical biosensors operating in secreted human physiological fluids, viz. tears, sweat, saliva, and urine. Described electrochemical devices are based on different electrochemical techniques, viz. amperometry, coulometry, cyclic voltammetry, and impedance spectroscopy. Challenges that confront researchers in this exciting area and key requirements for biodevices are discussed. It is concluded that the field of non-invasive sensing of biomarkers in bodily fluid is highly convoluted. Nonetheless, if the drawbacks are appropriately addressed, and the pitfalls are adroitly circumvented, the approach will most certainly disrupt current clinical and self-monitoring practices.

Place, publisher, year, edition, pages
MDPI, 2020
Keywords
non-invasive biosensors, human physiological fluids, tears, sweat, saliva, urine
National Category
Analytical Chemistry
Identifiers
urn:nbn:se:mau:diva-37103 (URN)10.3390/s20216352 (DOI)000589353300001 ()33171750 (PubMedID)2-s2.0-85096029841 (Scopus ID)
Available from: 2020-12-03 Created: 2020-12-03 Last updated: 2024-02-05Bibliographically approved
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