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Ohlsson, Lars
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Tassidis, H., Jankovskaja, S., Awad, K., Ohlsson, L., Gjörloff Wingren, A. & Gustafsson, A. (2024). Investigation of tryptophan to kynurenine degradation in response to interferon-γ in melanoma cell lines. Biochemistry and Biophysics Reports, 37, Article ID 101612.
Öppna denna publikation i ny flik eller fönster >>Investigation of tryptophan to kynurenine degradation in response to interferon-γ in melanoma cell lines
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2024 (Engelska)Ingår i: Biochemistry and Biophysics Reports, ISSN 2405-5808, Vol. 37, artikel-id 101612Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Background and aim: Melanoma is a fatal form of skin cancer that carries a grave prognosis if the cancer cells spread and form metastases. The Kynurenine (Kyn) pathway is activated by the enzyme indoleamine 2,3-dioxygenase 1 (IDO-1) and has been shown to have a role in tumour progression. We have previously shown that interferon-γ (IFN-γ) acts as an inducer of tryptophan (Trp) degradation to Kyn in keratinocytes of the basal layer in a 3D epidermis model. Before extending our reconstructed human epidermis model to not only contain keratinocytes but also fibroblasts and melanocytes/melanoma cells, we have in this study set out to investigate possible differences between primary adult melanocytes and six melanoma cell lines regarding the expression of the immune checkpoint inhibitors IDO-1 and programmed death ligand 1 (PD-L1) together with Kyn production.

Methods: The melanocytes and melanoma cells were stimulated with 1–20 ng/ml of IFN-γ and the levels of Trp to Kyn degradation were monitored with high-performance liquid chromatography (HPLC). To analyze the viability of the cell types after IFN-γ treatment, an MTT assay was performed. mRNA quantity of IDO-1, PD-L1 and IFN-γ receptor (IFN-GR1) was analyzed with qPCR.

Results: After 24 h, only the metastatic cell line WM-266-4 was affected by all concentrations of IFN-γ, whereas at 48 h, the higher IFN-γ concentrations gave a more pronounced effect on the viability in all cell types. Trp was detected at various levels in the culture medium from all cell types before and after IFN-γ treatment. The degradation to Kyn was detected in primary melanocytes, Mel Juso, and Mel Ho cell lines after 24 h of treatment and low levels of IFN-γ. However, the higher concentration of IFN-γ, 20 ng/ml, induced Kyn to various degrees in all cell types after 24 h. The change in mRNA quantity of IDO-1 and PD-L1 was similar in all cell types.

Conclusion: To conclude, no significant difference in upregulation of the immune checkpoint inhibitors PD-L1 and IDO-1 was seen between primary tumour and metastatic melanoma. IFN-γ stimulation of melanocytes and different stages of melanoma cell lines resulted in an increased Kyn/Trp ratio in the more aggressive melanoma cells when a high concentration was used (20 ng/ml) but when a lower concentration of IFN-γ (5 ng/ml) was used an increased Kyn/Trp ratio were detected in media from primary melanocytes and early-stage melanoma.

Ort, förlag, år, upplaga, sidor
Elsevier, 2024
Nyckelord
IDO-1, Interferon-γ, Kynurenine, Melanocytes, melanoma, Programmed death ligand 1, Tryptophan
Nationell ämneskategori
Cancer och onkologi
Identifikatorer
urn:nbn:se:mau:diva-64868 (URN)10.1016/j.bbrep.2023.101612 (DOI)001146252700001 ()38188364 (PubMedID)2-s2.0-85180557691 (Scopus ID)
Tillgänglig från: 2024-01-08 Skapad: 2024-01-08 Senast uppdaterad: 2024-02-27Bibliografiskt granskad
Falk, M., Psotta, C., Cirovic, S., Ohlsson, L. & Shleev, S. (2023). Electronic Tongue for Direct Assessment of SARS-CoV-2-Free and Infected Human Saliva-A Feasibility Study. Biosensors, 13(7), Article ID 717.
Öppna denna publikation i ny flik eller fönster >>Electronic Tongue for Direct Assessment of SARS-CoV-2-Free and Infected Human Saliva-A Feasibility Study
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2023 (Engelska)Ingår i: Biosensors, ISSN 2079-6374, Vol. 13, nr 7, artikel-id 717Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

An electronic tongue is a powerful analytical instrument based on an array of non-selective chemical sensors with a partial specificity for data gathering and advanced pattern recognition methods for data analysis. Connecting electronic tongues with electrochemical techniques for data collection has led to various applications, mostly within sensing for food quality and environmental monitoring, but also in biomedical research for the analyses of different bioanalytes in human physiological fluids. In this paper, an electronic tongue consisting of six electrodes (viz., gold, platinum, palladium, titanium, iridium, and glassy carbon) was designed and tested in authentic (undiluted, unpretreated) human saliva samples from eight volunteers, collected before and during the COVID-19 pandemic. Investigations of 11 samples using differential pulse voltammetry and a principal component analysis allowed us to distinguish between SARS-CoV-2-free and infected authentic human saliva. This work, as a proof-of-principle demonstration, provides a new perspective for the use of electronic tongues in the field of enzyme-free electrochemical biosensing, highlighting their potential for future applications in non-invasive biomedical analyses.

Ort, förlag, år, upplaga, sidor
MDPI, 2023
Nyckelord
electronic tongue, differential pulse voltammetry, principial component analysis, authentic human saliva, SARS-CoV-2
Nationell ämneskategori
Analytisk kemi
Identifikatorer
urn:nbn:se:mau:diva-61908 (URN)10.3390/bios13070717 (DOI)001038044400001 ()37504115 (PubMedID)2-s2.0-85165896609 (Scopus ID)
Tillgänglig från: 2023-08-16 Skapad: 2023-08-16 Senast uppdaterad: 2024-02-26Bibliografiskt granskad
Dao Nyesiga, G., Pool, L., Englezou, P. C., Hylander, T., Ohlsson, L., Appelgren, D., . . . Wigren, M. (2023). Tolerogenic dendritic cells generated in vitro using a novel protocol mimicking mucosal tolerance mechanisms represent a potential therapeutic cell platform for induction of immune tolerance.. Frontiers in Immunology, 14, Article ID 1045183.
Öppna denna publikation i ny flik eller fönster >>Tolerogenic dendritic cells generated in vitro using a novel protocol mimicking mucosal tolerance mechanisms represent a potential therapeutic cell platform for induction of immune tolerance.
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2023 (Engelska)Ingår i: Frontiers in Immunology, E-ISSN 1664-3224, Vol. 14, artikel-id 1045183Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Dendritic cells (DCs) are mediators between innate and adaptive immunity and vital in initiating and modulating antigen-specific immune responses. The most important site for induction of tolerance is the gut mucosa, where TGF-β, retinoic acid, and aryl hydrocarbon receptors collaborate in DCs to induce a tolerogenic phenotype. To mimic this, a novel combination of compounds – the synthetic aryl hydrocarbon receptor (AhR) agonist IGN-512 together with TGF-β and retinoic acid – was developed to create a platform technology for induction of tolerogenic DCs intended for treatment of several conditions caused by unwanted immune activation. These in vitro-generated cells, designated ItolDCs, are phenotypically characterized by their low expression of co-stimulatory and activating molecules along with high expression of tolerance-associated markers such as ILT3, CD103, and LAP, and a weak pro-inflammatory cytokine profile. When co-cultured with T cells and/or B cells, ItolDC-cultures contain higher frequencies of CD25+Foxp3+ regulatory T cells (Tregs), CD49b+LAG3+ ‘type 1 regulatory (Tr1) T cells, and IL-10-producing B cells and are less T cell stimulatory compared to cultures with matured DCs. Factor VIII (FVIII) and tetanus toxoid (TT) were used as model antigens to study ItolDC antigen-loading. ItolDCs can take up FVIII, process, and present FVIII peptides on HLA-DR. By loading both ItolDCs and mDCs with TT, antigen-specific T cell proliferation was observed. Cryo-preserved ItolDCs showed a stable tolerogenic phenotype that was maintained after stimulation with LPS, CD40L, or a pro-inflammatory cocktail. Moreover, exposure to other immune cells did not negatively impact ItolDCs’ expression of tolerogenic markers. In summary, a novel protocol was developed supporting the generation of a stable population of human DCs in vitro that exhibited a tolerogenic phenotype with an ability to increase proportions of induced regulatory T and B cells in mixed cultures. This protocol has the potential to constitute the base of a tolDC platform for inducing antigen-specific tolerance in disorders caused by undesired antigen-specific immune cell activation.

Ort, förlag, år, upplaga, sidor
Frontiers Media S.A., 2023
Nyckelord
antigen loading, antigen-specific response, cell therapy, immune tolerance, regulatory B cells (Bregs), regulatory T cells (Tregs), tolerogenic dendritic cells (tolDCs)
Nationell ämneskategori
Immunologi inom det medicinska området
Identifikatorer
urn:nbn:se:mau:diva-63661 (URN)10.3389/fimmu.2023.1045183 (DOI)001092545900001 ()37901231 (PubMedID)2-s2.0-85175369630 (Scopus ID)
Tillgänglig från: 2023-11-13 Skapad: 2023-11-13 Senast uppdaterad: 2024-04-19Bibliografiskt granskad
Beyer, S., Kimani, M., Zhang, Y., Verhassel, A., Sternbæk, L., Wang, T., . . . Stollenwerk, M. M. (2022). Fluorescent Molecularly Imprinted Polymer Layers against Sialic Acid on Silica-Coated Polystyrene Cores-Assessment of the Binding Behavior to Cancer Cells.. Cancers, 14(8), Article ID 1875.
Öppna denna publikation i ny flik eller fönster >>Fluorescent Molecularly Imprinted Polymer Layers against Sialic Acid on Silica-Coated Polystyrene Cores-Assessment of the Binding Behavior to Cancer Cells.
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2022 (Engelska)Ingår i: Cancers, ISSN 2072-6694, Vol. 14, nr 8, artikel-id 1875Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Sialic acid (SA) is a monosaccharide usually linked to the terminus of glycan chains on the cell surface. It plays a crucial role in many biological processes, and hypersialylation is a common feature in cancer. Lectins are widely used to analyze the cell surface expression of SA. However, these protein molecules are usually expensive and easily denatured, which calls for the development of alternative glycan-specific receptors and cell imaging technologies. In this study, SA-imprinted fluorescent core-shell molecularly imprinted polymer particles (SA-MIPs) were employed to recognize SA on the cell surface of cancer cell lines. The SA-MIPs improved suspensibility and scattering properties compared with previously used core-shell SA-MIPs. Although SA-imprinting was performed using SA without preference for the α2,3- and α2,6-SA forms, we screened the cancer cell lines analyzed using the lectins Maackia Amurensis Lectin I (MAL I, α2,3-SA) and Sambucus Nigra Lectin (SNA, α2,6-SA). Our results show that the selected cancer cell lines in this study presented a varied binding behavior with the SA-MIPs. The binding pattern of the lectins was also demonstrated. Moreover, two different pentavalent SA conjugates were used to inhibit the binding of the SA-MIPs to breast, skin, and lung cancer cell lines, demonstrating the specificity of the SA-MIPs in both flow cytometry and confocal fluorescence microscopy. We concluded that the synthesized SA-MIPs might be a powerful future tool in the diagnostic analysis of various cancer cells.

Ort, förlag, år, upplaga, sidor
MDPI, 2022
Nyckelord
SA conjugates, cancer, imprinting, molecularly imprinted polymers, sialic acid
Nationell ämneskategori
Biokemi och molekylärbiologi
Identifikatorer
urn:nbn:se:mau:diva-51285 (URN)10.3390/cancers14081875 (DOI)000786858400001 ()35454783 (PubMedID)2-s2.0-85127781436 (Scopus ID)
Tillgänglig från: 2022-05-04 Skapad: 2022-05-04 Senast uppdaterad: 2024-02-05Bibliografiskt granskad
Lindqvist, D., Furmark, T., Lavebratt, C., Ohlsson, L. & Månsson, K. N. (2022). Plasma circulating cell-free mitochondrial DNA in social anxiety disorder. Psychoneuroendocrinology, 148, Article ID 106001.
Öppna denna publikation i ny flik eller fönster >>Plasma circulating cell-free mitochondrial DNA in social anxiety disorder
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2022 (Engelska)Ingår i: Psychoneuroendocrinology, ISSN 0306-4530, E-ISSN 1873-3360, Vol. 148, artikel-id 106001Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

OBJECTIVE: To investigate plasma levels of circulating cell-free mitochondrial DNA (ccf-mtDNA) in patients with social anxiety disorder (SAD) and healthy controls (HC).

METHODS: In this study, 88 participants (46 patients with SAD and 42 HCs) were enrolled and both ccf-mtDNA and peripheral blood mononuclear cells (PBMC) mtDNA copy number (mtDNA-cn) were measured at up to three times per individual (9-11 weeks apart). SAD patients also received cognitive behavioral therapy (CBT) between the second and third time-point.

RESULTS: SAD patients had significantly lower ccf-mtDNA compared to HCs at all time points, but ccf-mtDNA did not change significantly after CBT, and was not associated with severity of anxiety symptoms. Plasma ccf-mtDNA did not significantly correlate with PBMC mtDNA-cn in patients.

CONCLUSION: This is the first report of lower ccf-mtDNA in patients with an anxiety disorder. Our findings could reflect a more chronic illness course in SAD patients with prolonged periods of psychological stress leading to decreased levels of ccf-mtDNA, but future longitudinal studies are needed to confirm or refute this hypothesis.

Ort, förlag, år, upplaga, sidor
Elsevier, 2022
Nyckelord
Circulating cell-free mitochondrial DNA, Cognitive behavioural therapy, Social anxiety disorder
Nationell ämneskategori
Psykiatri
Identifikatorer
urn:nbn:se:mau:diva-56603 (URN)10.1016/j.psyneuen.2022.106001 (DOI)000918010400006 ()36508952 (PubMedID)2-s2.0-85143981831 (Scopus ID)
Tillgänglig från: 2022-12-13 Skapad: 2022-12-13 Senast uppdaterad: 2024-02-05Bibliografiskt granskad
Fernström, J., Ohlsson, L., Asp, M., Lavant, E., Holck, A., Grudet, C., . . . Lindqvist, D. (2021). Plasma circulating cell-free mitochondrial DNA in depressive disorders. PLOS ONE, 16(11), Article ID e0259591.
Öppna denna publikation i ny flik eller fönster >>Plasma circulating cell-free mitochondrial DNA in depressive disorders
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2021 (Engelska)Ingår i: PLOS ONE, E-ISSN 1932-6203, Vol. 16, nr 11, artikel-id e0259591Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

BACKGROUND: Plasma circulating cell-free mitochondrial DNA (ccf-mtDNA) is an immunogenic molecule and a novel biomarker of psychiatric disorders. Some previous studies reported increased levels of ccf-mtDNA in unmedicated depression and recent suicide attempters, while other studies found unchanged or decreased ccf-mtDNA levels in depression. Inconsistent findings across studies may be explained by small sample sizes and between-study variations in somatic and psychiatric co-morbidity or medication status.

METHODS: We measured plasma ccf-mtDNA in a cohort of 281 patients with depressive disorders and 49 healthy controls. Ninety-three percent of all patients were treated with one or several psychotropic medications. Thirty-six percent had a personality disorder, 13% bipolar disorder. All analyses involving ccf-mtDNA were a priori adjusted for age and sex.

RESULTS: Mean levels in ccf-mtDNA were significantly different between patients with a current depressive episode (n = 236), remitted depressive episode (n = 45) and healthy controls (n = 49) (f = 8.3, p<0.001). Post-hoc tests revealed that both patients with current (p<0.001) and remitted (p = 0.002) depression had lower ccf-mtDNA compared to controls. Within the depressed group there was a positive correlation between ccf-mtDNA and "inflammatory depression symptoms" (r = 0.15, p = 0.02). We also found that treatment with mood stabilizers lamotrigine, valproic acid or lithium was associated with lower ccf-mtDNA (f = 8.1, p = 0.005).

DISCUSSION: Decreased plasma ccf-mtDNA in difficult-to-treat depression may be partly explained by concurrent psychotropic medications and co-morbidity. Our findings suggest that ccf-mtDNA may be differentially regulated in different subtypes of depression, and this hypothesis should be pursued in future studies.

Ort, förlag, år, upplaga, sidor
Public Library of Science, 2021
Nationell ämneskategori
Psykiatri
Identifikatorer
urn:nbn:se:mau:diva-46855 (URN)10.1371/journal.pone.0259591 (DOI)000755077100096 ()34735532 (PubMedID)2-s2.0-85118663333 (Scopus ID)
Tillgänglig från: 2021-11-15 Skapad: 2021-11-15 Senast uppdaterad: 2024-02-05Bibliografiskt granskad
Gustafsson, A., Prgomet, Z., Jankovskaja, S., Ruzgas, T., Engblom, J., Ohlsson, L. & Gjörloff Wingren, A. (2020). Effect of IFN-γ on the kynurenine/tryptophan ratio in monolayer-cultured keratinocytes and a 3D reconstructed human epidermis model. Journal of dermatological science (Amsterdam), 99(3), 177-184, Article ID S0923-1811(20)30234-6.
Öppna denna publikation i ny flik eller fönster >>Effect of IFN-γ on the kynurenine/tryptophan ratio in monolayer-cultured keratinocytes and a 3D reconstructed human epidermis model
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2020 (Engelska)Ingår i: Journal of dermatological science (Amsterdam), ISSN 0923-1811, E-ISSN 1873-569X, Vol. 99, nr 3, s. 177-184, artikel-id S0923-1811(20)30234-6Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

BACKGROUND: Interferon-gamma (IFN-γ) represents a potent inducer for keratinocyte inflammatory and immune activation in vitro. Since tryptophan (trp) conversion to kynurenine (kyn) is involved in inflammation, the topical kyn/trp ratio may serve as a biomarker of skin inflammation. However, the trp metabolism in keratinocytes exposed to IFN-γ is not yet fully understood.

OBJECTIVE: The aim of this study was to establish a human epidermis model in order to quantify cytokine and kyn/trp secretion from IFN-γ stimulated cells and tissues. Moreover, to compare the cell response of 2D-cultured keratinocytes and the 3D epidermis model.

METHODS: Polycarbonate filters were used on which primary keratinocytes could attach and stratify in order to form the typical layers of reconstructed human epidermis (RHE). After IFN-γ treatment, secretion of kyn/trp was measured by high performance liquid chromatography. Gene and protein expression of indoleamine 2,3-dioxygenase 1 (IDO) was analyzed with real-time PCR and immunohistochemistry. The secretion of cytokines was quantified with ELISA.

RESULTS: Trp catabolism to kyn was significantly increased (P < 0.01) in the 2D culture in response to IFN-γ treatment. Before kyn secretion, IDO was strongly upregulated (P < 0.001). IFN-γ treatment also increased the secretion of IL-6 and IL-8 from the keratinocytes. In the RHE, IDO was upregulated by IFN-γ, and kyn secretion could be detected. Interestingly, IDO expression was only present in the basal cells of the RHE.

CONCLUSION: Our results suggest that IFN-γ acts as an inducer of trp degradation preferentially in undifferentiated keratinocytes, indicated by the IDO expression in the basal layer of the RHE.

Ort, förlag, år, upplaga, sidor
Japanese Society for Investigative Dermatology, 2020
Nyckelord
IDO, Kynurenine, Pro-inflammatory cytokines, Reconstructed human epidermis, Tryptophan
Nationell ämneskategori
Medicin och hälsovetenskap
Identifikatorer
urn:nbn:se:mau:diva-18014 (URN)10.1016/j.jdermsci.2020.07.005 (DOI)000582365800005 ()32782183 (PubMedID)2-s2.0-85089257839 (Scopus ID)
Tillgänglig från: 2020-08-18 Skapad: 2020-08-18 Senast uppdaterad: 2024-02-05Bibliografiskt granskad
Ohlsson, L., Hall, A., Lindahl, H., Danielsson, R., Gustafsson, A., Lavant, E. & Ljunggren, L. (2020). Increased level of circulating cell-free mitochondrial DNA due to a single bout of strenuous physical exercise. European Journal of Applied Physiology, 120, 897-905
Öppna denna publikation i ny flik eller fönster >>Increased level of circulating cell-free mitochondrial DNA due to a single bout of strenuous physical exercise
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2020 (Engelska)Ingår i: European Journal of Applied Physiology, ISSN 1439-6319, E-ISSN 1439-6327, Vol. 120, s. 897-905Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Purpose Physical exercise is reported to affect the immune response in various ways. Thus, the levels of pro-inflammatory cytokines as well as the abundance of circulating leukocytes are changed. In this study, the occurence of circulating cell-free mitochondrial DNA (cfmtDNA) and nuclear DNA (nDNA) was investigated in connection with a single bout of strenuous physical exercise. Methods Healthy volunteers performed a controlled ergo-spirometry cycle test and venous blood samples were taken at different time-points to analyze the concentration of blood components before, during and after the test. The number of circulating leukocytes was measured, as well as secretion of the soluble urokinase activator receptor (suPAR). Results Cf-mtDNA significantly increased during exercise, compared to baseline values and after 30 and 90 min of rest. Circulating leukocytes increased during exercise, but returned to baseline levels afterwards. Surface expression of the urokinase plasminogen activating receptor (uPAR) on neutrophils decreased significantly during exercise. The concentration of suPAR tended to increase during exercise but only significantly after 90 min of rest. Conclusion Increased concentration of cf-mtDNA indicates that cell damage takes place during high intensity training. Hypoxia and tissue damage are likely causes of cf-mtDNA from muscle cells. The levels of cf-mtDNA remain high during the initial rest, due to the decreasing numbers of leukocytes normally clearing the plasma from cf-mtDNA. The increased levels of suPAR further emphasize that strenuous physical exercise causes a reaction similar to inflammation. Further studies are needed to detect the source of increased cf-mtDNA and the corresponding increase of suPAR liberation.

Ort, förlag, år, upplaga, sidor
Springer, 2020
Nationell ämneskategori
Fysiologi
Identifikatorer
urn:nbn:se:mau:diva-13796 (URN)10.1007/s00421-020-04330-8 (DOI)000516332900001 ()32088743 (PubMedID)2-s2.0-85079646397 (Scopus ID)
Tillgänglig från: 2020-03-17 Skapad: 2020-03-17 Senast uppdaterad: 2024-06-17Bibliografiskt granskad
El-Schich, Z., Zhang, Y., Feith, M., Beyer, S., Sternbæk, L., Ohlsson, L., . . . Gjörloff Wingren, A. (2020). Molecularly imprinted polymers in biological applications.. BioTechniques, 69(6)
Öppna denna publikation i ny flik eller fönster >>Molecularly imprinted polymers in biological applications.
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2020 (Engelska)Ingår i: BioTechniques, ISSN 0736-6205, E-ISSN 1940-9818, Vol. 69, nr 6Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Molecularly imprinted polymers (MIPs) are currently widely used and further developed for biological applications. The MIP synthesis procedure is a key process, and a wide variety of protocols exist. The templates that are used for imprinting vary from the smallest glycosylated glycan structures or even amino acids to whole proteins or bacteria. The low cost, quick preparation, stability and reproducibility have been highlighted as advantages of MIPs. The biological applications utilizing MIPs discussed here include enzyme-linked assays, sensors, in vivo applications, drug delivery, cancer diagnostics and more. Indeed, there are numerous examples of how MIPs can be used as recognition elements similar to natural antibodies

Ort, förlag, år, upplaga, sidor
Future Science, 2020
Nationell ämneskategori
Medicin och hälsovetenskap
Identifikatorer
urn:nbn:se:mau:diva-22017 (URN)10.2144/btn-2020-0091 (DOI)000575650700001 ()33000637 (PubMedID)2-s2.0-85097925056 (Scopus ID)
Tillgänglig från: 2020-10-28 Skapad: 2020-10-28 Senast uppdaterad: 2024-06-17Bibliografiskt granskad
Ohlsson, L., Gustafsson, A., Lavant, E., Suneson, K., Brundin, L., Westrin, Å., . . . Lindqvist, D. (2019). Leaky gut biomarkers in depression and suicidal behavior. (ed.). Acta Psychiatrica Scandinavica, 139(2), 185-193
Öppna denna publikation i ny flik eller fönster >>Leaky gut biomarkers in depression and suicidal behavior.
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2019 (Engelska)Ingår i: Acta Psychiatrica Scandinavica, ISSN 0001-690X, E-ISSN 1600-0447, Vol. 139, nr 2, s. 185-193Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

OBJECTIVE: Inflammation is associated with major depressive disorder (MDD) and suicidal behavior. According to the 'leaky gut hypothesis', increased intestinal permeability may contribute to this relationship via bacterial translocation across enterocytes. We measured plasma levels of gut permeability markers, in patients with a recent suicide attempt (rSA), MDD subjects with no history of a suicide attempt (nsMDD), and healthy controls (HC), and related these markers to symptom severity and inflammation. METHOD: We enrolled rSA (n = 54), nsMDD (n = 13), and HC (n = 17). Zonulin, intestinal fatty acid binding protein (I-FABP), soluble CD14, and interleukin-6 (IL-6) were quantified in plasma. Montgomery-Asberg Depression Rating Scale (MADRS) and Suicide Assessment Scale (SUAS) were used for symptom assessments. RESULTS: The rSA group displayed higher I-FABP and lower zonulin levels compared with both the nsMDD and the HC groups (all P < 0.001). IL-6 correlated positively with I-FABP (r = 0.24, P < 0.05) and negatively with zonulin (r = -0.25, P < 0.05). In all subjects, I-FABP levels correlated positively with MADRS (r = 0.25, P < 0.05) and SUAS scores (r = 0.38, P < 0.001), and the latter correlation was significant also in the nsMDD group (r = 0.60, P < 0.05). CONCLUSION: The 'leaky gut hypothesis' may improve our understanding of the link between inflammation and suicidal behavior. These findings should be considered preliminary until replicated in larger cohorts.

Ort, förlag, år, upplaga, sidor
John Wiley & Sons, 2019
Nationell ämneskategori
Medicin och hälsovetenskap
Identifikatorer
urn:nbn:se:mau:diva-5337 (URN)10.1111/acps.12978 (DOI)000456702900008 ()30347427 (PubMedID)2-s2.0-85055937078 (Scopus ID)27267 (Lokalt ID)27267 (Arkivnummer)27267 (OAI)
Tillgänglig från: 2020-02-28 Skapad: 2020-02-28 Senast uppdaterad: 2024-06-17Bibliografiskt granskad
Projekt
Icke-invasiv monitorering av hudsjukdomars progression och läkning baserat på lågmolekylära biomarkörer; Malmö universitetUtforskande av virusinteraktioner och humana celler; Malmö universitet
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