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Falk, Magnus
Publikationer (10 of 33) Visa alla publikationer
Figueiredo, C., Psotta, C., Jayakumar, K., Lielpetere, A., Mandal, T., Schuhmann, W., . . . De Lacey, A. L. (2024). Effect of Protection Polymer Coatings on the Performance of an Amperometric Galactose Biosensor in Human Plasma. Biosensors, 14(4), Article ID 167.
Öppna denna publikation i ny flik eller fönster >>Effect of Protection Polymer Coatings on the Performance of an Amperometric Galactose Biosensor in Human Plasma
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2024 (Engelska)Ingår i: Biosensors, ISSN 2079-6374, Vol. 14, nr 4, artikel-id 167Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Galactose monitoring in individuals allows the prevention of harsh health conditions related to hereditary metabolic diseases like galactosemia. Current methods of galactose detection need development to obtain cheaper, more reliable, and more specific sensors. Enzyme-containing amperometric sensors based on galactose oxidase activity are a promising approach, which can be enhanced by means of their inclusion in a redox polymer coating. This strategy simultaneously allows the immobilization of the biocatalyst to the electroactive surface and hosts the electron shuttling units. An additional deposition of capping polymers prevents external interferences like ascorbic or uric acid as well as biofouling when measuring in physiological fuels. This work studies the protection effect of poly(2-methacryloyloxyethyl phosphorylcholine-co-glycidyl methacrylate (MPC) and polyvinylimidazole-polysulfostyrene (P(VI-SS)) when incorporated in the biosensor design for the detection of galactose in human plasma.

Nyckelord
galactose, biosensor, plasma, protection polymers
Nationell ämneskategori
Kemi
Identifikatorer
urn:nbn:se:mau:diva-67293 (URN)10.3390/bios14040167 (DOI)001210296900001 ()38667160 (PubMedID)2-s2.0-85191463455 (Scopus ID)
Tillgänglig från: 2024-05-20 Skapad: 2024-05-20 Senast uppdaterad: 2024-05-20Bibliografiskt granskad
Psotta, C., Nilsson, E. J., Sjöberg, T. & Falk, M. (2023). Bacteria-Infected Artificial Urine Characterization Based on a Combined Approach Using an Electronic Tongue Complemented with 1H-NMR and Flow Cytometry. Biosensors, 13(10), 916-916
Öppna denna publikation i ny flik eller fönster >>Bacteria-Infected Artificial Urine Characterization Based on a Combined Approach Using an Electronic Tongue Complemented with 1H-NMR and Flow Cytometry
2023 (Engelska)Ingår i: Biosensors, E-ISSN 2079-6374, Vol. 13, nr 10, s. 916-916Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

The prevailing form of bacterial infection is within the urinary tract, encompassing a wide array of bacteria that harness the urinary metabolome for their growth. Through their metabolic actions, the chemical composition of the growth medium undergoes modifications as the bacteria metabolize urine compounds, leading to the subsequent release of metabolites. These changes can indirectly indicate the existence and proliferation of bacterial organisms. Here, we investigate the use of an electronic tongue, a powerful analytical instrument based on a combination of non-selective chemical sensors with a partial specificity for data gathering combined with principal component analysis, to distinguish between infected and non-infected artificial urine samples. Three prevalent bacteria found in urinary tract infections were investigated, Escherichia coli, Klebsiella pneumoniae, and Enterococcus faecalis. Furthermore, the electronic tongue analysis was supplemented with 1H NMR spectroscopy and flow cytometry. Bacteria-specific changes in compound consumption allowed for a qualitative differentiation between artificial urine medium and bacterial growth.

Ort, förlag, år, upplaga, sidor
MDPI, 2023
Nyckelord
electronic tongue, bacterial detection, artificial urine, urinary tract infection, 1H-NMR, flow cytometry
Nationell ämneskategori
Mikrobiologi
Identifikatorer
urn:nbn:se:mau:diva-63142 (URN)10.3390/bios13100916 (DOI)001096540000001 ()37887109 (PubMedID)2-s2.0-85175044453 (Scopus ID)
Tillgänglig från: 2023-10-13 Skapad: 2023-10-13 Senast uppdaterad: 2024-04-11Bibliografiskt granskad
Psotta, C., Cirovic, S., Gudmundsson, P., Falk, M., Mandal, T., Reichhart, T., . . . Shleev, S. (2023). Continuous ex vivo glucose sensing in human physiological fluids using an enzymatic sensor in a vein replica. Bioelectrochemistry, 152, Article ID 108441.
Öppna denna publikation i ny flik eller fönster >>Continuous ex vivo glucose sensing in human physiological fluids using an enzymatic sensor in a vein replica
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2023 (Engelska)Ingår i: Bioelectrochemistry, ISSN 1567-5394, E-ISSN 1878-562X, Vol. 152, artikel-id 108441Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Managing blood glucose can affect important clinical outcomes during the intraoperative phase of surgery. However, currently available instruments for glucose monitoring during surgery are few and not optimized for the specific application. Here we report an attempt to exploit an enzymatic sensor in a vein replica that could continuously monitor glucose level in an authentic human bloodstream. First, detailed investigations of the superficial venous systems of volunteers were carried out using ocular and palpating examinations, as well as advanced ultrasound measurements. Second, a tubular glucose-sensitive biosensor mimicking a venous system was designed and tested. Almost ideal linear dependence of current output on glucose concentration in phosphate buffer saline was obtained in the range 2.2-22.0 mM, whereas the dependence in human plasma was less linear. Finally, the developed biosensor was investigated in whole blood under homeostatic conditions. A specific correlation was found between the current output and glucose concentration at the initial stage of the biodevice operation. However, with time, blood coagulation during measurements negatively affected the performance of the biodevice. When the experimental results were remodeled to predict the response without the influence of blood coagulation, the sensor output closely followed the blood glucose level.

Ort, förlag, år, upplaga, sidor
Elsevier, 2023
Nyckelord
Continuous glucose sensing, Enzymatic sensor, Vein replica, Human physiological fluids, Surgery
Nationell ämneskategori
Endokrinologi och diabetes
Identifikatorer
urn:nbn:se:mau:diva-61052 (URN)10.1016/j.bioelechem.2023.108441 (DOI)000984583000001 ()37087795 (PubMedID)2-s2.0-85153044643 (Scopus ID)
Tillgänglig från: 2023-06-20 Skapad: 2023-06-20 Senast uppdaterad: 2024-04-19Bibliografiskt granskad
Falk, M., Psotta, C., Cirovic, S., Ohlsson, L. & Shleev, S. (2023). Electronic Tongue for Direct Assessment of SARS-CoV-2-Free and Infected Human Saliva-A Feasibility Study. Biosensors, 13(7), Article ID 717.
Öppna denna publikation i ny flik eller fönster >>Electronic Tongue for Direct Assessment of SARS-CoV-2-Free and Infected Human Saliva-A Feasibility Study
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2023 (Engelska)Ingår i: Biosensors, ISSN 2079-6374, Vol. 13, nr 7, artikel-id 717Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

An electronic tongue is a powerful analytical instrument based on an array of non-selective chemical sensors with a partial specificity for data gathering and advanced pattern recognition methods for data analysis. Connecting electronic tongues with electrochemical techniques for data collection has led to various applications, mostly within sensing for food quality and environmental monitoring, but also in biomedical research for the analyses of different bioanalytes in human physiological fluids. In this paper, an electronic tongue consisting of six electrodes (viz., gold, platinum, palladium, titanium, iridium, and glassy carbon) was designed and tested in authentic (undiluted, unpretreated) human saliva samples from eight volunteers, collected before and during the COVID-19 pandemic. Investigations of 11 samples using differential pulse voltammetry and a principal component analysis allowed us to distinguish between SARS-CoV-2-free and infected authentic human saliva. This work, as a proof-of-principle demonstration, provides a new perspective for the use of electronic tongues in the field of enzyme-free electrochemical biosensing, highlighting their potential for future applications in non-invasive biomedical analyses.

Ort, förlag, år, upplaga, sidor
MDPI, 2023
Nyckelord
electronic tongue, differential pulse voltammetry, principial component analysis, authentic human saliva, SARS-CoV-2
Nationell ämneskategori
Analytisk kemi
Identifikatorer
urn:nbn:se:mau:diva-61908 (URN)10.3390/bios13070717 (DOI)001038044400001 ()37504115 (PubMedID)2-s2.0-85165896609 (Scopus ID)
Tillgänglig från: 2023-08-16 Skapad: 2023-08-16 Senast uppdaterad: 2024-02-26Bibliografiskt granskad
Psotta, C., Chaturvedi, V., Gonzalez-Martinez, J. F., Sotres, J. & Falk, M. (2023). Portable Prussian Blue-Based Sensor for Bacterial Detection in Urine. Sensors, 23(1), Article ID 388.
Öppna denna publikation i ny flik eller fönster >>Portable Prussian Blue-Based Sensor for Bacterial Detection in Urine
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2023 (Engelska)Ingår i: Sensors, E-ISSN 1424-8220, Vol. 23, nr 1, artikel-id 388Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Bacterial infections can affect the skin, lungs, blood, and brain, and are among the leading causes of mortality globally. Early infection detection is critical in diagnosis and treatment but is a time- and work-consuming process taking several days, creating a hitherto unmet need to develop simple, rapid, and accurate methods for bacterial detection at the point of care. The most frequent type of bacterial infection is infection of the urinary tract. Here, we present a wireless-enabled, portable, potentiometric sensor for E. coli. E. coli was chosen as a model bacterium since it is the most common cause of urinary tract infections. The sensing principle is based on reduction of Prussian blue by the metabolic activity of the bacteria, detected by monitoring the potential of the sensor, transferring the sensor signal via Bluetooth, and recording the output on a laptop or a mobile phone. In sensing of bacteria in an artificial urine medium, E. coli was detected in similar to 4 h (237 +/- 19 min; n = 4) and in less than 0.5 h (21 +/- 7 min, n = 3) using initial E. coli concentrations of similar to 10(3) and 10(5) cells mL(-1), respectively, which is under or on the limit for classification of a urinary tract infection. Detection of E. coli was also demonstrated in authentic urine samples with bacteria concentration as low as 10(4) cells mL(-1), with a similar response recorded between urine samples collected from different volunteers as well as from morning and afternoon urine samples.

Ort, förlag, år, upplaga, sidor
MDPI, 2023
Nyckelord
portable sensing, bacterial detection, Prussian blue, urine analysis
Nationell ämneskategori
Analytisk kemi
Identifikatorer
urn:nbn:se:mau:diva-58385 (URN)10.3390/s23010388 (DOI)000908806900001 ()36616986 (PubMedID)2-s2.0-85145976536 (Scopus ID)
Tillgänglig från: 2023-02-27 Skapad: 2023-02-27 Senast uppdaterad: 2024-02-05Bibliografiskt granskad
Carlson, E., Stigmar, M., Engberg, M., Falk, M., Stollenwerk, M. M., Gudmundsson, P. & Enskär, K. (2022). Students´ Experiences of Participation in a Research Team: Evaluation of a Research-based Teaching Activity in HigherEducation. International Journal for the Scholarship of Teaching & Learning, 16(3)
Öppna denna publikation i ny flik eller fönster >>Students´ Experiences of Participation in a Research Team: Evaluation of a Research-based Teaching Activity in HigherEducation
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2022 (Engelska)Ingår i: International Journal for the Scholarship of Teaching & Learning, E-ISSN 1931-4744, Vol. 16, nr 3Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

AbstractIn Sweden as well as internationally the teaching and research nexus has been described as the defining charac-teristics of higher education promoting generic skills such as information analysis and critical reflection. Vertically Integrated Projects has been proposed as one educational strategy where research and teaching are linked by in-viting students to take active part in actual research projects. The strategy is well aligned to Scholarship of teaching and learning enabling the transition from a teacher-centred accepted knowledge to a student-centred perspective where students are invited as producers of knowledge. The aim of the current study was to explore students’ experiences of participation in a research-based learning activity with academia and industrial partners, designed as a qualitative explorative study using focus group interviews. Findings describe not only factors students find motivating for learning, but also their experience of being part of professional life with its benefits and challenges.

Ort, förlag, år, upplaga, sidor
Faculty Center at Georgia Southern University, 2022
Nyckelord
Focus group, Interdisciplinary, Research-based learning, Students, Vertically Integrated Project
Nationell ämneskategori
Utbildningsvetenskap
Identifikatorer
urn:nbn:se:mau:diva-56407 (URN)10.20429/ijsotl.2022.160306 (DOI)
Tillgänglig från: 2022-12-01 Skapad: 2022-12-01 Senast uppdaterad: 2024-05-07Bibliografiskt granskad
Falk, M., Nilsson, E. J., Cirovic, S., Tudosoiu, B. & Shleev, S. (2021). Wearable Electronic Tongue for Non-Invasive Assessment of Human Sweat. Sensors, 21(21), Article ID 7311.
Öppna denna publikation i ny flik eller fönster >>Wearable Electronic Tongue for Non-Invasive Assessment of Human Sweat
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2021 (Engelska)Ingår i: Sensors, E-ISSN 1424-8220, Vol. 21, nr 21, artikel-id 7311Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Sweat is a promising biofluid in allowing for non-invasive sampling. Here, we investigate the use of a voltammetric electronic tongue, combining different metal electrodes, for the purpose of non-invasive sample assessment, specifically focusing on sweat. A wearable electronic tongue is presented by incorporating metal electrodes on a flexible circuit board and used to non-invasively monitor sweat on the body. The data obtained from the measurements were treated by multivariate data processing. Using principal component analysis to analyze the data collected by the wearable electronic tongue enabled differentiation of sweat samples of different chemical composition, and when combined with 1H-NMR sample differentiation could be attributed to changing analyte concentrations.

Ort, förlag, år, upplaga, sidor
MDPI, 2021
Nyckelord
electronic tongue, human sweat, non-invasive analysis, wearable sensors
Nationell ämneskategori
Analytisk kemi
Identifikatorer
urn:nbn:se:mau:diva-46850 (URN)10.3390/s21217311 (DOI)000719077700001 ()34770617 (PubMedID)2-s2.0-85118345498 (Scopus ID)
Tillgänglig från: 2021-11-15 Skapad: 2021-11-15 Senast uppdaterad: 2024-02-05Bibliografiskt granskad
Falk, M., Psotta, C., Cirovic, S. & Shleev, S. (2020). Non-Invasive Electrochemical Biosensors Operating in Human Physiological Fluids. Sensors, 20(21), 1-28, Article ID 6352.
Öppna denna publikation i ny flik eller fönster >>Non-Invasive Electrochemical Biosensors Operating in Human Physiological Fluids
2020 (Engelska)Ingår i: Sensors, E-ISSN 1424-8220, Vol. 20, nr 21, s. 1-28, artikel-id 6352Artikel, forskningsöversikt (Refereegranskat) Published
Abstract [en]

Non-invasive healthcare technologies are an important part of research and development nowadays due to the low cost and convenience offered to both healthcare receivers and providers. This work overviews the recent advances in the field of non-invasive electrochemical biosensors operating in secreted human physiological fluids, viz. tears, sweat, saliva, and urine. Described electrochemical devices are based on different electrochemical techniques, viz. amperometry, coulometry, cyclic voltammetry, and impedance spectroscopy. Challenges that confront researchers in this exciting area and key requirements for biodevices are discussed. It is concluded that the field of non-invasive sensing of biomarkers in bodily fluid is highly convoluted. Nonetheless, if the drawbacks are appropriately addressed, and the pitfalls are adroitly circumvented, the approach will most certainly disrupt current clinical and self-monitoring practices.

Ort, förlag, år, upplaga, sidor
MDPI, 2020
Nyckelord
non-invasive biosensors, human physiological fluids, tears, sweat, saliva, urine
Nationell ämneskategori
Analytisk kemi
Identifikatorer
urn:nbn:se:mau:diva-37103 (URN)10.3390/s20216352 (DOI)000589353300001 ()33171750 (PubMedID)2-s2.0-85096029841 (Scopus ID)
Tillgänglig från: 2020-12-03 Skapad: 2020-12-03 Senast uppdaterad: 2024-02-05Bibliografiskt granskad
Shleev, S., Aleksejeva, O., Falk, M. & Blum, Z. (2019). Biodegradable electric power devices. In: Serge Cosnier (Ed.), Bioelectrochemistry: Design and Applications of Biomaterials (pp. 237-258). Walter de Gruyter
Öppna denna publikation i ny flik eller fönster >>Biodegradable electric power devices
2019 (Engelska)Ingår i: Bioelectrochemistry: Design and Applications of Biomaterials / [ed] Serge Cosnier, Walter de Gruyter, 2019, s. 237-258Kapitel i bok, del av antologi (Övrigt vetenskapligt)
Ort, förlag, år, upplaga, sidor
Walter de Gruyter, 2019
Nationell ämneskategori
Annan elektroteknik och elektronik
Identifikatorer
urn:nbn:se:mau:diva-44438 (URN)10.1515/9783110570526-012 (DOI)2-s2.0-85096739550 (Scopus ID)9783110568981 (ISBN)9783110570526 (ISBN)
Tillgänglig från: 2021-06-30 Skapad: 2021-06-30 Senast uppdaterad: 2024-02-05Bibliografiskt granskad
Shleev, S., Falk, M., Cirovic, S. & Blum, Z. (2019). Wearable bioelectronic devices. In: Serge Cosnier (Ed.), Bioelectrochemistry: Design and Applications of Biomaterials (pp. 213-236). Walter de Gruyter
Öppna denna publikation i ny flik eller fönster >>Wearable bioelectronic devices
2019 (Engelska)Ingår i: Bioelectrochemistry: Design and Applications of Biomaterials / [ed] Serge Cosnier, Walter de Gruyter, 2019, s. 213-236Kapitel i bok, del av antologi (Övrigt vetenskapligt)
Ort, förlag, år, upplaga, sidor
Walter de Gruyter, 2019
Nationell ämneskategori
Annan elektroteknik och elektronik
Identifikatorer
urn:nbn:se:mau:diva-44436 (URN)10.1515/9783110570526-011 (DOI)2-s2.0-85096729246 (Scopus ID)9783110568981 (ISBN)9783110570526 (ISBN)
Tillgänglig från: 2021-06-30 Skapad: 2021-06-30 Senast uppdaterad: 2024-02-05Bibliografiskt granskad
Projekt
Icke-invasiva biomedicinska multiparameterenheter: synliggörande av dolda tränings- och hälsoindikatorer; Malmö universitetHögpresterande kostnadseffektiva fotoelektriska biosuperkondensatorer för industriell tillverkning; Malmö universitet
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