Publikationer från Malmö universitet
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  • 1. Braune, W
    et al.
    Ekelund, Nils
    Institutionen för Fysiologisk Botanik, Lunds Universitet.
    Phototactic responses in Haematococcus lacustris and its modification by light intensity and the carotenoid biosynthesis inhibitor Norflurazon1990Inngår i: Archives of Microbiology, ISSN 0302-8933, E-ISSN 1432-072X, Vol. 154, s. 448-452Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    At fluence rates below 45 W· m-2 cells of the flagellate stage of Haematococcus lacustris react only positively phototactically with a rather high degree of orientation (indicated by r values up to 0.66 with the Rayleigh test). The directedness of orientation decreases with decreasing irradiance. The degree of directedness of the phototactic response depends on the intensity of preirradiation: Low light intensity applied after strong light application results in a “dark reaction” (low r values), low light given after darkness stimulates a rather high degree of directedness of positive phototaxis. Weak blue light (λ=483 nm; 0.4 W · m-2) stimulates positive phototactic response, whereas comparable red light (λ=658 nm; 0.5 W · m-2) does not.

  • 2.
    Ekelund, Nils
    Department of Plant Physiology, Lund University.
    Effects of calcium channel blockers and DCMU on motility and the photophobic response of Gyrodinium dorsum1989Inngår i: Archives of Microbiology, ISSN 0302-8933, E-ISSN 1432-072X, Vol. 151, s. 187-190Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    The effects of the calcium channel blockers, verapamil, diltiazem and lanthanum ions and the Ca2+ dependency on motility as well as the photophobic response (stop-response) of Gyrodinium dorsum were studied. At Ca2+ concentrations below 10-3 M, motility was inhibited. La3+ inhibits the stop-response, in contrast to verapamil and diltiazem. The only calcium channel blocker that increased the amount of non-motile cells was verapamil. The results indicate that motility are Ca2+ dependent and that the stop-responses of G. dorsum could be affected by extracellular Ca2+. Effects of the photosythesis inhibitor (DCMU) on the stop-response was also determined. With background light of different wavelength (614, 658 and 686 nm) the stop-response increased. DCMU inhibited this effect of background light. Negative results with the monoclonal antibody Pea-25 directed to phytochrome and the results with DCMU, indicate that the stop-response of G. dorsum is coupled to photosynthesis rather than to a phytochrome-like pigment. Oxygen evolution, but not cell movement, was completely inhibited by 10-6 M DCMU.

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