Publikationer från Malmö universitet
Endre søk
Begrens søket
1 - 6 of 6
RefereraExporteraLink til resultatlisten
Permanent link
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
Treff pr side
  • 5
  • 10
  • 20
  • 50
  • 100
  • 250
Sortering
  • Standard (Relevans)
  • Forfatter A-Ø
  • Forfatter Ø-A
  • Tittel A-Ø
  • Tittel Ø-A
  • Type publikasjon A-Ø
  • Type publikasjon Ø-A
  • Eldste først
  • Nyeste først
  • Skapad (Eldste først)
  • Skapad (Nyeste først)
  • Senast uppdaterad (Eldste først)
  • Senast uppdaterad (Nyeste først)
  • Disputationsdatum (tidligste først)
  • Disputationsdatum (siste først)
  • Standard (Relevans)
  • Forfatter A-Ø
  • Forfatter Ø-A
  • Tittel A-Ø
  • Tittel Ø-A
  • Type publikasjon A-Ø
  • Type publikasjon Ø-A
  • Eldste først
  • Nyeste først
  • Skapad (Eldste først)
  • Skapad (Nyeste først)
  • Senast uppdaterad (Eldste først)
  • Senast uppdaterad (Nyeste først)
  • Disputationsdatum (tidligste først)
  • Disputationsdatum (siste først)
Merk
Maxantalet träffar du kan exportera från sökgränssnittet är 250. Vid större uttag använd dig av utsökningar.
  • 1.
    Alshammari, Hatem
    Malmö universitet, Odontologiska fakulteten (OD).
    Antimicrobial potential of strontium against bacteria associated with peri-implantitis2023Doktoravhandling, med artikler (Annet vitenskapelig)
    Abstract [en]

    Dental implants are nowadays a standard treatment to replace missing teeth and restore function and aesthetics. However, biological complications associated with implants, e.g., peri-implant infections, are common and jeopardize the success of treatment. The main aim of this thesis was to explore strontium (Sr) as a possible prevention strategy against peri-implant infections, since Sr has been shown to have antibacterial action and also to promote titanium (Ti) implant osseointegration. In this thesis, a systematic appraisal of the literature about the antimicrobial potential of Sr-functionalized Ti surfaces for oral applications was performed, and was followed by a series of in vitro studies assessing the antimicrobial potential of Sr against micro-organisms associated with peri-implantitis.

    In Study I, the systematic appraisal of the literature resulted in an initial list of 1081 potentially relevant publications, where from nine publications from in vitro studies met the inclusion criteria. Most of the included studies showed that Sr-functionalized Ti exerted a limited immediate (i.e., 24 h) antimicrobial effect, likely due to a low Sr ion release; a relevant antimicrobial effect and biofilm inhibition potential against Streptococcus aureus was observed at both early and late timepoints, with an adequate Sr ion release.

    Study II assessed, in vitro, five different concentrations of soluble Sr(OH)2 (100, 10, 1, 0.1, and 0.01 mM) against 6 different mono-species bacteria (Streptococcusmitis, Staphylococcus epidermidis, Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Escherichia coli, and Fusobacterium nucleatum) in terms of cell growth, minimal inhibitory concentration (MIC), and biofilm viability. In the agar diffusion test, zones of inhibition were only observed for 0.01, 0.1, and 1 mM of Sr(OH)2 against P. gingivalis. Growth inhibition in planktonic cultures was achieved at 10 mM for all species tested. In the biofilm viability assay, 10 and 100 mM Sr(OH)2 showed potent bactericidal effect against S. mitis, S. epidermidis, A. actinomycetemcomitans, E. coli, and P. gingivalis. It was thus concluded that Sr(OH)2 has antimicrobial properties against bacteria associated with peri-implantitis.

    Study III assessed the in vitro early- and late bacteriostatic and bactericidal effect of Sr-functionalized wafers on bacteria associated with peri-implantitis (E. coli,S. aureus, Streptococcus oralis, Actinomyces naeslundii, Parvimonas micra, P.gingivalis and F. nucleatum) as mono-species after 2 and 24 hours, and as multispecies at day 1, 3, and 6. Sr-functionalized wafers, compared to Ti controls, were associated with statistically significant less viable cells in both mono- and multispecies tests. Number of colony forming units (CFUs) within the biofilm were significantly higher in Ti wafers, compared to Sr-functionalized wafers, for S. aureus at all time-points of evaluation and for E. coli at day 1. Gingipain activity was higher in Ti wafers compared to Sr-functionalized ones, and the qPCR showed that P. gingivalis comprised 15% of the total biofilm on Ti wafers at day 6, while it remained below detection levels at Sr-coated wafers.

    In Study IV, the impact of Ti surface roughness (turned vs moderately rough) on the antimicrobial effect of Sr on bacterial associated with peri-implantitis (S. oralis, P. micra, A. naeslundii, F. nucleatum, P. gingivalis, S. aureus, and E. coli), grown in different multispecies consortia, was assessed in vitro. Bacterial viability and biofilm formation, and well as, proteolytic activity of P. gingivalis were assessed at day 1, 3, and 6. Sr-functionalized surfaces were associated with statistically significant reduction in number of viable cells compared to nonfunctionalized surface at all times of investigation for all multispecies tested. Higher proteolytic activity of P. gingivalis was found at non-functionalized Ti disks compared to Sr-functionalized ones. Sr-functionalised surfaces were associated with notable growth inhibition of both E. coli and S. aureus, while P. gingivalis remined undetected at all time points of evaluation on all disks. The turned surface had a slightly higher release of Sr ion compared with the moderately rough surface in the first 24 hours, while both surfaces showed a sustained release for up to 15 days.

    Overall, the data generated with this series of projects indicate Sr surfaces exerts an antimicrobial potential on bacteria associated with peri-implantitis and it is worthwhile to further explore the potential of Sr-functionalized Ti in the prevention of peri-implant infections.

    Fulltekst (pdf)
    fulltext
    Download (jpg)
    presentationsbild
  • 2.
    Alshammari, Hatem
    Malmö universitet, Odontologiska fakulteten (OD).
    Antimicrobial Potential of Strontium Functionalized Titanium Against Bacteria Associated with Periimplantitis.Manuskript (preprint) (Annet vitenskapelig)
    Abstract [en]

    Aim: to explore the antimicrobial potential of strontium (Sr) coated wafers against multiple bacteria associated with per-implant infections, in both mono and multi-species forms.

    Materials and methods: Sr-coated and uncoated Ti wafers were tested for bactericidal effect and biofilm growth inhibition against several bacteria, either grown as a mono-species or as a culture of multi-species, using bacterial viability assay and plate counting method. Mono-species test performed over a rocking platform and evaluated after 2 and 24 hours, while multi-species antimicrobial test evaluation was carried out at day 1, 3 and 6. The impact of Sr coating on the total percentage of P. gingivalis in the multi-species biofilm, using qPCR, and gingipain activity were also assessed.

    Results: Sr-coated wafers were associated with statistically significant less viable cells for both mono- and multi-species tests compared to Ti controls. Number of colony forming units (CFUs) were significantly higher in Ti wafers, for S. aureus, at all time-points of evaluation and at day 1 for E. coli within the biofilm compared to Sr-coated wafers. Gingipains activity were higher in Ti wafers compared to Sr-coated ones, and qPCR showed that P. gingivalis percentage was 15% of the total biofilm of Ti wafers at day 6, while it remained below detection levels at Sr-coated wafers.

    Conclusion: Sr coating displayed promising antimicrobial potential against bacteria associated with peri-implantitis grown either as mono-species or in a multi-species consortium.

  • 3.
    Alshammari, Hatem
    Malmö universitet, Odontologiska fakulteten (OD).
    Impact of Strontium Functionalized Moderately Rough vs. Turned Titanium Disks on Biofilm Formation and Bacterial ViabilityManuskript (preprint) (Annet vitenskapelig)
    Abstract [en]

    Aim: To study the effect of Sr coated Ti disks with smooth and moderately rough surface on biofilm formation, gingipain activity, inhibition of growth of Porphyromonas gingivalis, Escherichia coli and Staphylococcus aureus using amulti-species model mimicking the subgingival environment, and to explore the pattern of Sr ion release.

    Materials and methods: the antimicrobial potential of Sr coated disks, i.e., smoothSr (S-Sr) and rough Sr (R-Sr) was investigated using a five species bacterial model and compared to uncoated Ti disks, i.e., smooth Ti (S-Ti) and rough Ti (RTi)at 3 different time point: 1, 3 and 6 days. Biofilm formation was investigated using LIVE/DEAD BacLight staining and confocal scanning laser microscopy and image analysis software, while inhibition of growth of P. gingivalis, E. coli and S. aureus was measured using qPCRr or growth on selective agar medium. Gingipain activity was measured using fluorescent substrate BIKKAM-16. Sr ion release from Sr coated disks was investigated up to 15 days.

    Results: S-Sr and R-Sr were associated with statistically significant reduction in number of viable cells compared to S-Ti and R-Ti at all different times of investigation for all multispecies tested (p < 0.001). Higher proteolytic activity associated with uncoated Ti disks compared to Sr coated disks in the P. gingivalis multispecies test at day 6. Utilizing qPCR, P. gingivalis remined undetected at all time points of evaluation for all disks, while in plate counting method, coated surfaces were associated with notable growth inhibition of both E.coil and S.aureus, using MacConkey and 110-plates, respectively. S-Sr had slightly higher release of Sr ion in the first 24 hours than R-Sr, while both Sr coated surfaces sustained stable release up to 15 days.

    Conclusion: In the present study, Sr coated surface, i.e., S-Sr and R-Sr, demonstrated great antimicrobial potential against multiple bacterial associated with peri-implant infection and provided a possible strategy for Ti implants infection prevention.

  • 4.
    Alshammari, Hatem
    et al.
    Malmö universitet, Odontologiska fakulteten (OD). University of Hail, Saudi Arabia.
    Bakitian, Fahad
    Umm Al-Qura University, Saudi Arabia.
    Neilands, Jessica
    Malmö universitet, Odontologiska fakulteten (OD).
    Andersen, Ole Zoffmann
    Elos Medtech, Denmark..
    Stavropoulos, Andreas
    Malmö universitet, Odontologiska fakulteten (OD). University of Geneva, Switzerland.
    Antimicrobial Properties of Strontium Functionalized Titanium Surfaces for Oral Applications, A Systematic Review2021Inngår i: Coatings, ISSN 2079-6412, Vol. 11, nr 7, artikkel-id 810Artikkel, forskningsoversikt (Fagfellevurdert)
    Abstract [en]

    The aim of this systematic review was to assess the current scientific evidence of the antimicrobial potential of strontium (Sr) when used to functionalize titanium (Ti) for oral applications. Out of an initial list of 1081 potentially relevant publications identified in three electronic databases (MEDLINE via PubMed, Scopus, and Cochrane) up to 1 February 2021, nine publications based on in vitro studies met the inclusion criteria. The antimicrobial potential of Sr was investigated on different types of functionalized Ti substrates, employing different application methods. Nine studies reported on the early, i.e., 6-24 h, and two studies on the late, i.e., 7-28 days, antimicrobial effect of Sr, primarily against Staphylococcus aureus (S. aureus) and/or Escherichia coli (E. coli). Sr-modified samples demonstrated relevant early antimicrobial potential against S. aureus in three studies; only one of which presented statistical significance values, while the other two presented only the percentage of antimicrobial rate and biofilm inhibition. A relevant late biofilm inhibition potential against S. aureus of 40% and 10%-after 7 and 14 days, respectively-was reported in one study. Combining Sr with other metal ions, i.e., silver (Ag), zinc (Zn), and fluorine (F), demonstrated a significant antimicrobial effect and biofilm inhibition against both S. aureus and E. coli. Sr ion release within the first 24 h was generally low, i.e., below 50 mu g/L and 0.6 ppm; however, sustained Sr ion release for up to 30 days, while maintaining up to 90% of its original content, was also demonstrated. Thus, in most studies included herein, Sr-functionalized Ti showed a limited immediate (i.e., 24 h) antimicrobial effect, likely due to a low Sr ion release; however, with an adequate Sr ion release, a relevant antimicrobial effect, as well as a biofilm inhibition potential against S. aureus-but not E. coli-was observed at both early and late timepoints. Future studies should assess the antimicrobial potential of Ti functionalized with Sr against multispecies biofilms associated with peri-implantitis.

    Fulltekst (pdf)
    fulltext
  • 5.
    Alshammari, Hatem
    et al.
    Malmö universitet, Odontologiska fakulteten (OD).
    Neilands, Jessica
    Malmö universitet, Odontologiska fakulteten (OD). Malmö universitet, Biofilms Research Center for Biointerfaces.
    Svensäter, Gunnel
    Malmö universitet, Odontologiska fakulteten (OD). Malmö universitet, Biofilms Research Center for Biointerfaces.
    Stavropoulos, Andreas
    Malmö universitet, Odontologiska fakulteten (OD). University of Geneva, Switzerland.
    Antimicrobial Potential of Strontium Hydroxide on Bacteria Associated with Peri-Implantitis2021Inngår i: Antibiotics, ISSN 0066-4774, E-ISSN 2079-6382, Vol. 10, nr 2, artikkel-id 150Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Background: Peri-implantitis due to infection of dental implants is a common complication that may cause significant patient morbidity. In this study, we investigated the antimicrobial potential of Sr(OH)2 against different bacteria associated with peri-implantitis. Methods: The antimicrobial potential of five concentrations of Sr(OH)2 (100, 10, 1, 0.1, and 0.01 mM) was assessed with agar diffusion test, minimal inhibitory concentration (MIC), and biofilm viability assays against six bacteria commonly associated with biomaterial infections: Streptococcus mitis, Staphylococcus epidermidis, Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Escherichia coli, and Fusobacterium nucleatum. Results: Zones of inhibition were only observed for, 0.01, 0.1, and 1 mM of Sr(OH)2 tested against P. gingivalis, in the agar diffusion test. Growth inhibition in planktonic cultures was achieved at 10 mM for all species tested (p < 0.001). In biofilm viability assay, 10 and 100 mM Sr(OH)2 showed potent bactericidal affect against S. mitis, S. epidermidis, A. actinomycetemcomitans, E. coli, and P. gingivalis. Conclusions: The findings of this study indicate that Sr(OH)2 has antimicrobial properties against bacteria associated with peri-implantitis. 

    Fulltekst (pdf)
    fulltext
  • 6.
    Bertl, Kristina
    et al.
    Malmö universitet, Odontologiska fakulteten (OD).
    Geissberger, Chiarra
    Univ Bern, Sch Dent Med, Dept Periodontol, Bern, Switzerland..
    Zinndorf, David
    Univ Bern, Sch Dent Med, Dept Periodontol, Bern, Switzerland..
    Edlund Johansson, Pia
    Malmö universitet, Odontologiska fakulteten (OD).
    Al-Shammari, Hatem
    Malmö universitet, Odontologiska fakulteten (OD).
    Eick, Sigrun
    Univ Bern, Sch Dent Med, Dept Periodontol, Bern, Switzerland..
    Stavropoulos, Andreas
    Malmö universitet, Odontologiska fakulteten (OD).
    Bacterial colonisation during regular daily use of a power-driven water flosser and risk for cross-contamination. Can it be prevented?2022Inngår i: Clinical Oral Investigations, ISSN 1432-6981, E-ISSN 1436-3771, Vol. 26, s. 1903-1913Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Objective To assess whether bacterial colonisation in a power-driven water flosser can be prevented. Materials and methods Twenty-four patients undergoing supportive periodontal treatment used 2 power-driven water flossers [Sonicare AirFloss (SAF), AirFloss Ultra (SAFU)] for 12 weeks each as follows: (a) with bottled water (BW); (b) with BW and cleaning the device extra-orally twice per week with chlorhexidine gluconate or (c) essential-oil-based (EO) mouth-rinse; (d) with EO only. Water-jet samples were taken after 6 and 12 weeks with the used nozzle and after exchanging to a brand-new nozzle. After 12 weeks, all devices underwent an intensive cleaning procedure. Samples were analysed by PCR-based method for cariogenic and periodontal pathogens and culture for staphylococci, aerobe gram-negative bacteria, and Candida sp. Results Contamination of SAF/SAFU with Streptococcus mutans was found in > 95% of the samples; periodontal pathogens and aerobe gram-negative bacteria were detected in 19-56% of the samples, while Staphylococcus aureus and Candida sp. were identified only in few samples. Contamination rate was basically unaffected by time-point, device, or way of use. Further, exchanging the nozzle did not prevent transmission of a contaminated water-jet, but the intensive cleaning reduced most of the pathogens significantly, except of S. mutans. Conclusion Neither a specific way of use nor exchanging the nozzle prevented bacterial colonisation and transmission of biofilm components via the water-jet of SAF/SAFU.

    Fulltekst (pdf)
    fulltext
1 - 6 of 6
RefereraExporteraLink til resultatlisten
Permanent link
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf