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  • 1.
    Davies, Julia R
    et al.
    Malmö universitet, Odontologiska fakulteten (OD). Malmö universitet, Biofilms Research Center for Biointerfaces.
    Kad, Trupti
    Malmö universitet, Odontologiska fakulteten (OD). Malmö universitet, Biofilms Research Center for Biointerfaces.
    Neilands, Jessica
    Malmö universitet, Odontologiska fakulteten (OD). Malmö universitet, Biofilms Research Center for Biointerfaces.
    Kinnby, B
    Malmö universitet, Odontologiska fakulteten (OD). Malmö universitet, Biofilms Research Center for Biointerfaces.
    Prgomet, Zdenka
    Malmö universitet, Odontologiska fakulteten (OD). Malmö universitet, Biofilms Research Center for Biointerfaces.
    Bengtsson, Torbjörn
    School of Medical Sciences, Örebro University, Örebro, Sweden..
    Khalaf, Hazem
    School of Medical Sciences, Örebro University, Örebro, Sweden..
    Svensäter, Gunnel
    Malmö universitet, Odontologiska fakulteten (OD). Malmö universitet, Biofilms Research Center for Biointerfaces.
    Polymicrobial synergy stimulates Porphyromonas gingivalis survival and gingipain expression in a multi-species subgingival community.2021Ingår i: BMC Oral Health, E-ISSN 1472-6831, Vol. 21, nr 1, artikel-id 639Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    BACKGROUND: Dysbiosis in subgingival microbial communities, resulting from increased inflammatory transudate from the gingival tissues, is an important factor in initiation and development of periodontitis. Dysbiotic communities are characterized by increased numbers of bacteria that exploit the serum-like transudate for nutrients, giving rise to a proteolytic community phenotype. Here we investigate the contribution of interactions between members of a sub-gingival community to survival and development of virulence in a serum environment-modelling that in the subgingival pocket.

    METHODS: Growth and proteolytic activity of three Porphyromonas gingivalis strains in nutrient broth or a serum environment were assessed using A600 and a fluorescent protease substrate, respectively. Adherence of P. gingivalis strains to serum-coated surfaces was studied with confocal microscopy and 2D-gel electrophoresis of bacterial supernatants used to investigate extracellular proteins. A model multi-species sub-gingival community containing Fusobacterium nucleatum, Streptococcus constellatus, Parvimonas micra with wild type or isogenic mutants of P. gingivalis was then created and growth and proteolytic activity in serum assessed as above. Community composition over time was monitored using culture techniques and qPCR.

    RESULTS: The P. gingivalis strains showed different growth rates in nutrient broth related to the level of proteolytic activity (largely gingipains) in the cultures. Despite being able to adhere to serum-coated surfaces, none of the strains was able to grow alone in a serum environment. Together in the subgingival consortium however, all the included species were able to grow in the serum environment and the community adopted a proteolytic phenotype. Inclusion of P. gingivalis strains lacking gingipains in the consortium revealed that community growth was facilitated by Rgp gingipain from P. gingivalis.

    CONCLUSIONS: In the multi-species consortium, growth was facilitated by the wild-type and Rgp-expressing strains of P. gingivalis, suggesting that Rgp is involved in delivery of nutrients to the whole community through degradation of complex protein substrates in serum. Whereas they are constitutively expressed by P. gingivalis in nutrient broth, gingipain expression in the model periodontal pocket environment (serum) appeared to be orchestrated through signaling to P. gingivalis from other members of the community, a phenomenon which then promoted growth of the whole community.

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  • 2.
    Hall, Jan
    et al.
    The Brånemark Clinic, Public Dental Health Service, Västra Götaland, Gothenburg, Sweden.
    Neilands, Jessica
    Malmö universitet, Odontologiska fakulteten (OD). Malmö universitet, Biofilms Research Center for Biointerfaces.
    Davies, Julia R
    Malmö universitet, Odontologiska fakulteten (OD). Malmö universitet, Biofilms Research Center for Biointerfaces.
    Ekestubbe, Annika
    Department of Oral and Maxillofacial Radiology, Institute of Odontology, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.
    Friberg, Bertil
    The Brånemark Clinic, Public Dental Health Service, Västra Götaland, Gothenburg, Sweden.
    A randomized, controlled, clinical study on a new titanium oxide abutment surface for improved healing and soft tissue health2019Ingår i: Clinical Implant Dentistry and Related Research, ISSN 1523-0899, E-ISSN 1708-8208, Vol. 21, nr Suppl 1, s. 55-68Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Background: A newly developed, anodized titanium oxide surface containing anatase has been reported to have antimicrobial properties that could reduce bacterial adherence to abutments. Purpose: To investigate if abutments with the anodized surface improve healing and soft tissue health in a randomized controlled study. Materials and Methods: Test abutments with a nanostructured anodized surface were compared with control machined titanium abutments. In total, 35 subjects each received a pair of test and control abutments. The primary endpoint was reduction of biofilm formation at test abutments at the 6‐week follow‐up. Secondary endpoints included several soft tissue assessments. qPCR for gene markers was used to indirectly evaluate healing and soft tissue health. Results: No significant differences in biofilm formation were observed between test and control abutments, but soft tissue bleeding upon abutment removal was significantly lower for test abutments compared with control abutments (P = 0.006) at 6 weeks. Keratinized mucosa height was significantly greater at test abutments compared with control abutments at the 6‐week, 6‐month, and 2‐year follow‐ups. Significant gene expression differences indicated differences in healing and tissue remodeling. Conclusions: Abutments with an anodized and nanostructured surface compared with a conventional, machined titanium surface had no significant effect on bacterial colonization and proteolytic activity but were associated with better soft tissue outcomes such as a lower bleeding index at abutment removal and consistently greater height of keratinized mucosa throughout the 2‐year follow‐up, suggesting improved surface‐dependent peri‐implant healing and soft tissue health.

  • 3.
    Kinnby, Bertil
    Malmö högskola, Odontologiska fakulteten (OD).
    Multifaceted plasminogen2016Ingår i: Archives of Oral Biology, ISSN 0003-9969, E-ISSN 1879-1506, Vol. 74, s. 133-135Artikel i tidskrift (Refereegranskat)
  • 4.
    Kinnby, Bertil
    et al.
    Malmö högskola, Odontologiska fakulteten (OD).
    Booth, Nuala A
    Svensäter, Gunnel
    Malmö högskola, Odontologiska fakulteten (OD).
    Plasminogen binding by oral streptococci from dental plaque and inflammatory lesions2008Ingår i: Microbiology, ISSN 1350-0872, E-ISSN 1465-2080, Vol. 154, nr 3, s. 924-931Artikel i tidskrift (Övrig (populärvetenskap, debatt, mm))
    Abstract [en]

    Plasminogen binding by bacteria is a virulence factor important for the entry and dissemination of bacteria in the body. A wide variety of bacteria bind plasminogen, including both organisms causing disease and components of the normal oral flora. The purpose of this study was to examine the characteristics of plasminogen binding by six clinical isolates of oral streptococci from both dental plaque and inflammatory lesions. All the strains bound plasminogen with approximately the same affinity, and binding was specific and lysine-dependent as evidenced by its inhibition by epsilon-aminocaproic acid. All of the test strains were capable of activating bound plasminogen to plasmin without the addition of a plasminogen activator, and subsequent analysis revealed the presence of streptokinase in all strains. However, the streptococci exhibited fibrinolytic activity only in the presence of plasminogen and this could be inhibited by the addition of epsilon-aminocaproic acid. SDS-PAGE and 2D gel electrophoresis coupled with plasminogen ligand blotting showed that only a subset of the total proteins (2-15) were involved in the binding of plasminogen. Partial identification of the binding proteins revealed that four glycolytic enzymes, enolase, phosphoglycerate kinase, glyceraldehyde-3-phosphate dehydrogenase and phosphoglycerate mutase, were predominant in binding plasminogen. The binding of plasminogen by bacteria from pus did not differ from that of the strains from supragingival plaque. The findings illustrate how apparently innocuous commensal bacteria are capable of utilizing a mechanism that is generally regarded as being of importance to pathogenicity and suggest an additional role of plasminogen binding.

  • 5.
    Kinnby, Bertil
    et al.
    Malmö högskola, Odontologiska fakulteten (OD).
    Chavez de Paz, Luis E.
    Malmö högskola, Odontologiska fakulteten (OD).
    Plasminogen coating increases initial adhesion of oral bacteria in vitro2016Ingår i: Microbial Pathogenesis, ISSN 0882-4010, E-ISSN 1096-1208, Vol. 100, s. 10-16Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Plasminogen is a major plasma protein and the zymogen of the broad spectrum protease plasmin. Plasmin activity leads to tissue degradation, direct and through activation of metalloproteinases. Infected tooth root canals, as a consequence of the inflammatory response and eventual necrosis, contain tissue fluid and blood components. These will coat the root canal walls and act as conditioning films that allow bacterial biofilms to grow and be a potential source of hematogenously spreading bacteria. We investigated the effect of in vitro surface conditioning with human plasminogen on the initial adhesion of bacteria. Four bacterial species, L. salivarius, E. faecalis, A. naeslundii, and S. gordonii, isolated from dental root canals, and three other oral streptococci, S. oralis, S. anginosus, and S. sanguinis, were grown in albumin- or plasminogen-coated flow chambers and studied by confocal laser scanning microscopy using the cell viability staining LIVE/DEAD and 16S rRNA fluorescence in situ hybridization (FISH). A. naeslundii, L. salivarius and in particular S. gordonii showed a higher initial adhesion to the plasminogen-coated surfaces. E. faecalis did not show any preference for plasminogen. Four-species biofilms cultured for 96 h showed that streptococci increased their proportion with time. Further experiments aimed at studying different streptococcal strains. All these adhered more to plasminogen-coated surfaces than to albumin-coated control surfaces. The specificity of the binding to plasminogen was verified by blocking lysine-binding sites with epsilon-aminocaproic acid. Plasminogen is thus an important plasma component for the initial adhesion of oral bacteria, in particular streptococci. This binding may contribute to their spread locally as well as to distant organs or tissues.

  • 6.
    Lindberg, Pia
    et al.
    Malmö högskola, Odontologiska fakulteten (OD).
    Billström, Anita
    Kinnby, Bertil
    Malmö högskola, Odontologiska fakulteten (OD).
    Reduced Gingival Fluid Flow: a Peripheral Marker of the Pharmacological Effect of Roquinimex2003Ingår i: InflammoPharmacology, ISSN 0925-4692, E-ISSN 1568-5608, Vol. 11, nr 3, s. 267-276Artikel i tidskrift (Övrigt vetenskapligt)
    Abstract [en]

    OBJECTIVE: Roquinimex is a drug with effects on inflammation and tumors. The pharmacological effect is not fully understood, and the molecular mechanism most characterized in vitro is an increase of plasminogen activator inhibitor type 2 (PAI-2) in human peripheral blood monocytes. The aims were to investigate peripheral pharmacological effects of roquinimex on peripheral blood monocytes and dog gingival fluid (GCF). DESIGN: Six dogs were used in a cross-over study. The amount of GCF was determined with a Periotron. The PAI-2 concentration in GCF was determined with ELISA. Monocytes were isolated from peripheral blood. RESULTS: Dogs treated with the drug had significantly lower GCF flow values and the PAI-2 concentration in GCF was higher, but no effect was seen on peripheral monocytes. CONCLUSION: Roquinimex treatment led to a consistently decreased flow rate of GCF and a higher local concentration of PAI-2 in GCF.

  • 7.
    Neilands, Jessica
    et al.
    Malmö högskola, Odontologiska fakulteten (OD).
    Bikker, Floris
    Department of Oral Biochemistry, Academic Centre for Dentistry Amsterdam, Free University and University of Amsterdam, Amsterdam, The Netherlands.
    Kinnby, Bertil
    Malmö högskola, Odontologiska fakulteten (OD).
    PAI-2/SerpinB2 Inhibits Proteolytic Activity in a P. gingivalis-dominated Multispecies Bacterial Consortium2016Ingår i: Archives of Oral Biology, ISSN 0003-9969, E-ISSN 1879-1506, Vol. 70, s. 1-8Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Objective The aim of this study was to investigate the ability of the serine protease inhibitor plasminogen activator inhibitor type 2 (PAI-2/Serpin B2) to inhibit proteases produced by a multispecies bacterial consortium in vitro. Background Gingival and periodontal inflammation is associated with an increased flow of protein-rich gingival fluid. This nutritional change in the microenvironment favors bacteria with a proteolytic phenotype, triggering inflammation and associated tissue breakdown. PAI-2 is produced by macrophages and keratinocytes and is present in very high concentrations in gingival crevicular fluid; the highest level in the body. Design A multispecies bacterial consortium comprising nine bacterial strains, resembling the conditions in a periodontal pocket, was grown planktonically and as a biofilm. After seven days PAI-2 was added to the consortium and the proteolytic activity was assayed with fluorogenic protease substrates; FITC-labeled casein to detect global protease activity, fluorescent H-Gly-Pro-AMC for serine protease activity and fluorescent BIKKAM-10 for Porphyromonas gingivalis-associated protease activity. Protease activity associated with biofilm cells was examined by confocal scanning laser microscopy. Results PAI-2 inhibited proteolytic activity of the bacterial consortium, as seen by decreased fluorescence of all substrates. PAI-2 specifically inhibited P. gingivalis proteolytic activity. Conclusion To our knowledge, this is the first time that PAI-2 has been shown to inhibit bacterial proteases. Given the high concentration of PAI-2 in the gingival region, our results indicate that PAI-2 might play a role for the integrity of the epithelial barrier.

  • 8.
    Neilands, Jessica
    et al.
    Malmö universitet, Odontologiska fakulteten (OD).
    Kinnby, B
    Malmö universitet, Odontologiska fakulteten (OD). Malmö universitet, Biofilms Research Center for Biointerfaces.
    Porphyromonas gingivalis initiates coagulation and secretes polyphosphates: A mechanism for sustaining chronic inflammation?2022Ingår i: Microbial Pathogenesis, ISSN 0882-4010, E-ISSN 1096-1208, Vol. 162, s. 1-8, artikel-id 104648Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    BACKGROUND: Periodontitis is a chronic inflammation resulting in destruction of tooth-supporting bone. Chronic inflammation is characterized by extravascular fibrin deposition. Fibrin is central to destruction of bone; monocytes bind to fibrin and form osteoclasts, thus providing a link between coagulation and the tissue destructive processes in periodontitis. The oral microbiome is essential to oral health. However, local ecological changes, such as increased biofilm formation, result in a dysbiotic microbiome characterized by an increase of protease-producing species e.g. Porphyromonas gingivalis. Proteases initiate inflammation and may cleave coagulation factors. Polyphosphates (polyP) may also provide bacteria with procoagulant properties similar to platelet-released polyP. P. gingivalis has also been found in remote locations related to vascular pathology and Alzheimer's disease.

    OBJECTIVES: The aim of this study was to investigate procoagulant activity of ten different species of oral bacteria present in oral health and disease as well as presence of polyP and fibrin formation in planktonic and biofilm bacteria.

    METHODS: Oral bacteria were studied for protease production and procoagulant activity. The presence of polyP and formation of fibrin was observed using confocal microscopy.

    RESULTS: P. gingivalis showed strong protease activity and was the only species exerting procoagulant activity. Confocal microscopy showed polyP intracellularly in planktonic bacteria and extracellularly after biofilm formation. Fibrin formation emanated from planktonic bacteria and from both bacteria and polyP in biofilm cultures.

    CONCLUSIONS: The procoagulant activity of P. gingivalis could explain its role in chronic inflammation, locally in oral tissues as well as in remote locations.

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  • 9.
    Neilands, Jessica
    et al.
    Malmö högskola, Odontologiska fakulteten (OD).
    Wickström, Claes
    Malmö högskola, Odontologiska fakulteten (OD).
    Kinnby, Bertil
    Malmö högskola, Odontologiska fakulteten (OD).
    Davies, Julia R
    Malmö högskola, Odontologiska fakulteten (OD).
    Hall, Jan
    Friberg, Bertil
    Malmö högskola, Odontologiska fakulteten (OD).
    Svensäter, Gunnel
    Malmö högskola, Odontologiska fakulteten (OD).
    Bacterial profiles and proteolytic activity in peri-implantitis versus healthy sitesBacterial profiles and proteolytic activity in peri-implantitis versus healthy sites2015Ingår i: Anaerobe, ISSN 1075-9964, E-ISSN 1095-8274, Vol. 35, s. 28-34Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Peri-implantitis is a biofilm-induced destructive inflammatory process that, over time, results in loss of supporting bone around an osseointegrated dental implant. Biofilms at peri-implantitis sites have been reported to be dominated by Gram-negative anaerobic rods with a proteolytic metabolism such as, Fusobacterium, Porphyromonas, Prevotella and Tannerella, as well as anaerobic Gram-positive cocci. In this study, we hypothesized that protease activity is instrumental in driving bone destruction and we therefore compared the microbial composition and level of protease activity in samples of peri-implant biofluid (PIBF) from 25 healthy subjects (H group) and 25 subjects with peri-implantitis (PI group). Microbial composition was investigated using culture techniques and protease activity was determined using a FITC-labelled casein substrate. The microbial composition was highly variable in subjects both in the H and PI groups but one prominent difference was the prevalence of Porphyromonas/Prevotella and anaerobic Gram positive cocci which was significantly higher in the PI than in the H group. A subgroup of subjects with peri-implantitis displayed a high level of protease activity in the PIBF compared to healthy subjects. However, this activity could not be related to the presence of specific bacterial species. We propose that a high level of protease activity may be a predictive factor for disease progression in peri-implantitis. Further longitudinal studies are however required to determine whether assessment of protease activity could serve as a useful method to identify patients at risk for progressive tissue destruction.

  • 10.
    Olofsson, A
    et al.
    Malmö högskola, Odontologiska fakulteten (OD).
    Lindberg, Pia
    Malmö högskola, Odontologiska fakulteten (OD).
    Lanke, J
    Matsson, Lars
    Malmö högskola, Odontologiska fakulteten (OD).
    Kinnby, B
    Malmö högskola, Odontologiska fakulteten (OD).
    Relationship between fibrinolytic activity and gingival inflammatory reaction in young individuals2003Ingår i: Journal of Periodontal Research, ISSN 0022-3484, E-ISSN 1600-0765, Vol. 38, nr 1, s. 104-108Artikel i tidskrift (Övrigt vetenskapligt)
    Abstract [en]

    The fibrinolytic system (the plasminogen activating system) is involved in several physiological and pathological processes. Through the transformation of plasminogen to the aggressive broad spectrum protease plasmin, potent enzymatic activity is released. Plasmin acts directly on connective tissue components, and indirectly by activating proforms of the metalloproteinases. The destructive potential of the fibrinolytic system may thus be of importance for the initiation and progression of periodontal diseases. Earlier studies have shown high concentrations of the plasminogen activator t-PA and its inhibitor PAI-2 in gingival crevicular fluid (GCF) as well as enhanced concentrations in areas of gingival inflammation. The aim of this study was to investigate a possible relationship between the gingival inflammatory reactivity and the fibrinolytic activity in gingival crevicular fluid. Thirty-one young individuals took part in the study. Gingival Index scores and Plaque Index scores were assessed and used to formulate a score expressing an individuals’ inflammatory response to microbial plaque levels (Relative G/P score). The fibrinolytic activity of GCF was assessed with a fibrin gel lysis assay, and the levels of t-PA and PAI-2 were assayed with ELISAs. All samples showed fibrinolytic activity. A positive correlation between the fibrinolytic activity and Relative G/P score was found. Thus, in individuals with an enhanced reactivity to dental plaque, a higher plasminogen activating activity in GCF was seen. This indicates a higher potential for tissue proteolysis in these individuals, possibly facilitating spread and deeper involvement of the lesions.

  • 11.
    Pihl, M
    et al.
    Malmö högskola, Odontologiska fakulteten (OD).
    Arnebrant, Thomas
    Malmö högskola, Fakulteten för hälsa och samhälle (HS).
    Kinnby, B
    Malmö högskola, Odontologiska fakulteten (OD).
    Svensäter, Gunnel
    Malmö högskola, Odontologiska fakulteten (OD).
    Peritoneal dialysis catheter biofilms.2008Konferensbidrag (Övrigt vetenskapligt)
  • 12. Rabe, Per
    et al.
    Twetman, Svante
    Kinnby, Bertil
    Malmö högskola, Odontologiska fakulteten (OD). Malmö högskola, Biofilms Research Center for Biointerfaces.
    Svensäter, Gunnel
    Malmö högskola, Odontologiska fakulteten (OD). Malmö högskola, Biofilms Research Center for Biointerfaces.
    Davies, Julia R
    Malmö högskola, Odontologiska fakulteten (OD). Malmö högskola, Biofilms Research Center for Biointerfaces.
    Effect of fluoride and chlorhexidine digluconate mouthrinses on plaque biofilms2015Ingår i: The Open Dentistry Journal, E-ISSN 1874-2106, Vol. 9, s. 106-111Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Objective. To develop a model in which to investigate the architecture of plaque biofilms formed on enamel surfaces in vivo and to compare the effects of anti-microbial agents of relevance for caries on biofilm vitality. Materials and Methodology : Enamel discs mounted on healing abutments in the pre-molar region were worn by three subjects for 7 days. Control discs were removed before subjects rinsed with 0.1% chlorhexidine digluconate (CHX) or 0.2% sodium fluoride (NaF) for 1 minute. Biofilms were stained with Baclight Live/Dead and z-stacks of images created using confocal scanning laser micoscopy. The levels of vital and dead/damaged bacteria in the biofilms, assessed as the proportion of green and red pixels respectively, were analysed using ImageTrak(®) software. Results : The subjects showed individual differences in biofilm architecture. The thickness of the biofilms varied from 28-96µm although cell density was always the greatest in the middle layers. In control biofilms, the overall levels of vitality were high (71-98%) especially in the area closest to the enamel interface. Rinsing with either CHX or NaF caused a similar reduction in overall vitality. CHX exerted an effect throughout the biofilm, particularly on the surface of cell clusters whereas NaF caused cell damage/death mainly in the middle to lower biofilm layers. Conclusion : We describe a model that allows the formation of mature, undisturbed oral biofilms on human enamel surfaces in vivo and show that CHX and NaF have a similar effect on overall vitality but differ in their sites of action.

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  • 13.
    Sonesson, Mikael
    et al.
    Malmö högskola, Odontologiska fakulteten (OD).
    Ericson, Dan
    Malmö högskola, Odontologiska fakulteten (OD).
    Kinnby, Bertil
    Malmö högskola, Odontologiska fakulteten (OD).
    Wickström, Claes
    Malmö högskola, Odontologiska fakulteten (OD).
    Glycoprotein 340 and sialic acid in minor-gland and whole saliva of children, adolescents, and adults2011Ingår i: European Journal of Oral Sciences, ISSN 0909-8836, E-ISSN 1600-0722, Vol. 6, nr 119, s. 435-440Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Glycoprotein 340 (gp-340) is a bacterial-binding glycoprotein observed in major and minor gland saliva. Sialic acid, a common terminal structure of salivary glycoproteins, interacts with microorganisms and host ligands, as well as free radicals. This study investigated the content of gp-340 and sialic acid in minor gland and whole saliva of children (3-yr-olds), adolescents (14-yr-olds) and adults (20 to 25-yr-olds). Labial and buccal gland saliva was collected on filter paper and un-stimulated whole saliva by draining into a tube. The relative amount of gp-340 and sialic acid was determined by ELISA and ELLA, respectively. In minor gland saliva, no statistically significant differences in gp-340 and sialic acid were seen between the age-groups. Significantly lower amounts of gp-340 and sialic acid were seen in labial saliva compared to buccal among adults (P < 0.05, P < 0.01). In whole saliva, the amount of gp-340 was significantly lower among adults compared to children (P < 0.01). No differences between genders were seen. Stable gp-340 and sialic acid contents in minor-gland saliva across the age-groups and higher gp-340 content in the whole saliva of the youngest age-group (3-yr-olds) compared with the adult-group may reflect a vital innate factor of immunity in children’s saliva.

  • 14.
    Sonesson, Mikael
    et al.
    Malmö högskola, Odontologiska fakulteten (OD).
    Wickström, Claes
    Malmö högskola, Odontologiska fakulteten (OD).
    Kinnby, Bertil
    Malmö högskola, Odontologiska fakulteten (OD).
    Ericson, Dan
    Malmö högskola, Odontologiska fakulteten (OD).
    Matsson, Lars
    Malmö högskola, Odontologiska fakulteten (OD).
    Mucins MUC5B and MUC7 in minor salivary gland secretion of children and adults2008Ingår i: Archives of Oral Biology, ISSN 0003-9969, E-ISSN 1879-1506, Vol. 53, nr 6, s. 523-527Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    OBJECTIVE: The study was designed to investigate the relative amount of MUC5B and MUC7 in minor salivary glands in children and adults, in order to test the hypothesis that secretion of salivary mucins changes between childhood and adulthood. METHODS: Ninety individuals in the age-groups 3-year-olds, 14-year-olds, and young adults 20-25 year-olds were recruited. Sialopapers were applied on the labial and the buccal mucosa and then placed in the Periotron 8000 (Proflowtrade mark) for calculation of the amount of saliva. The assessment of MUC5B and MUC7 was carried out in an ELISA using the LUM5B-2 and the LUM7-1 antiserum, respectively. RESULTS: MUC5B and MUC7 were detected in the labial minor gland saliva in all age groups. In buccal gland saliva, only a few individuals in each age group showed detectable amounts of the mucins. In the labial area, a significantly lower level of MUC7 was noted in 3-year-olds compared with adults. CONCLUSION: The results indicate a site-dependent difference in minor gland mucin secretion and an age-related difference in the labial gland secretion of MUC7.

  • 15. Ullbro, Christer
    et al.
    Kinnby, Bertil
    Malmö högskola, Odontologiska fakulteten (OD).
    Lindberg, Pia
    Malmö högskola, Odontologiska fakulteten (OD).
    Matsson, Lars
    Malmö högskola, Odontologiska fakulteten (OD).
    Tissue Plasminogen Activator (t-PA) and Placental Plasminogen Activator Inhibitor (PAI-2) in Gingival Crevicular Fluid from Patients with Papillon-Lefevre Syndrome2004Ingår i: Journal of Clinical Periodontology, ISSN 0303-6979, E-ISSN 1600-051X, Vol. 31, nr 9, s. 708-712Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    OBJECTIVES: Numerous patients with Papillon-Lefevre syndrome (PLS) express a severe periodontal inflammation that results in prema-ture loss of deciduous and permanent teeth. The plasminogen activat-ing (PA) system is involved in physiological and pathological processes including epithelial healing, extracellular proteolysis and local in-flammatory reactions. The aim of the study was to explore a possible role of the PA system in patients with PLS. MATERIAL AND METHODS: Samples of gingival crevicular fluid (GCF) were collected from areas with gingival infection in 20 patients with PLS and in 20 healthy controls. The concentration of tissue plasminogen activator (t-PA) and inhibitor (PAI-2) was measured with ELISA. RESULTS: The median level of PAI-2 was significantly higher (p < 0.01) in PLS pa-tients than in the controls, while the median value of t-PA did not dif-fer between the groups. No difference in t-PA or PAI-2 levels was found regarding age, gender or presence of active periodontal disease. CONCLUSION: The findings indicate an atypical activity of the PA system with a disturbed epithelial function in PLS patients, suggesting that the periodontal destruction seen in patients with PLS is secondary to a hereditary defect in the defense system.

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