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  • 1.
    Engberg, Agnes
    Malmö University, Faculty of Health and Society (HS), Department of Biomedical Science (BMV).
    Förbättrad art- och underartbestämning av Streptococcus bovis/Streptococcus equinus-komplexet med MALDI-TOF MS2022Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
    Abstract [en]

    The Streptococcus bovis/Streptococcus equinus complex (SBSEC) includes seven species and subspecies of commensal and opportunistic pathogenic bacteria found in humans, domesticated livestock, and wild animals. The wide range of phenotypic expression within the complex has resulted in repeated name changes and reclassifications in the past hundred years. Infection with SBSEC is rare but associated with severe conditions such as bacteremia and endocarditis. There is an established association between infection with certain subspecies and colon cancer. Bruker MALDI-TOF MS Biotyper is used for bacterial species identification of clinical patient samples in Region Skåne. Identification of members of SBSEC is currently not available with high resolution to subspecies level, and with insufficient accuracy. There is a need for proper identification of SBSEC subspecies as it has clinical implications to the affected patients. New reference libraries that can replace or complement existing libraries from Bruker are evidently needed. A group of reference isolates, with previously determined species and subspecies through whole genome sequencing, were cultured under aerobic and anaerobic conditions and constituted a new library. Brukers library and the newly created library were evaluated and compared by a group of identified test isolates cultured under aerobic and anaerobic conditions (n = 51).The results showed a significant improvement (p < 0.0001) in accurate species and subspecies identification with MALDI-TOF MS from 67% with Brukers library to 100% with the new library.

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