Publikationer från Malmö universitet
Endre søk
Begrens søket
1 - 46 of 46
RefereraExporteraLink til resultatlisten
Permanent link
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
Treff pr side
  • 5
  • 10
  • 20
  • 50
  • 100
  • 250
Sortering
  • Standard (Relevans)
  • Forfatter A-Ø
  • Forfatter Ø-A
  • Tittel A-Ø
  • Tittel Ø-A
  • Type publikasjon A-Ø
  • Type publikasjon Ø-A
  • Eldste først
  • Nyeste først
  • Skapad (Eldste først)
  • Skapad (Nyeste først)
  • Senast uppdaterad (Eldste først)
  • Senast uppdaterad (Nyeste først)
  • Disputationsdatum (tidligste først)
  • Disputationsdatum (siste først)
  • Standard (Relevans)
  • Forfatter A-Ø
  • Forfatter Ø-A
  • Tittel A-Ø
  • Tittel Ø-A
  • Type publikasjon A-Ø
  • Type publikasjon Ø-A
  • Eldste først
  • Nyeste først
  • Skapad (Eldste først)
  • Skapad (Nyeste først)
  • Senast uppdaterad (Eldste først)
  • Senast uppdaterad (Nyeste først)
  • Disputationsdatum (tidligste først)
  • Disputationsdatum (siste først)
Merk
Maxantalet träffar du kan exportera från sökgränssnittet är 250. Vid större uttag använd dig av utsökningar.
  • 1.
    Ademovski, Emir
    Malmö universitet, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV).
    In vitro effects of skincare ingredients on keratinocytes2023Independent thesis Basic level (degree of Bachelor), 10 poäng / 15 hpOppgave
    Abstract [en]

    The skin has several functions as the largest and one of the most complex organs of the body. One of the skin’s primary functions is to prevent water loss by retaining water to allow the skin to function in dry environments. The outermost layer, stratum corneum (SC), retains water loss as rehydration by natural moisturizing factors (NMFs). In this project, HaCaT cells were incubated with commonly used skincare ingredients such as urea, glycerol, transcutol, salicylic acid, and polyethylene glycol 4000 Da (PEG-4000) to evaluate their impact on cell viability, MTT proliferation assay and gene expression measurements by qPCR. The relationship between cell viability, gene expression, and water activity was also studied. The excipients showed a dose-dependent decrease in cell viability because osmotic pressure increased. One finding was that transcutol exhibited a protective effect against concentrations where osmotic pressure harmed the cells. PEG-4000, with a concentration of 10 % (w/v), showed an upregulation of elongation of very long chain fatty acid 4 (ELOVL-4). Gene expression of serine palmitoyltransferase (SPT) was low, with 10 mM transcutol, even though the cells had a viability of >100 %. It should have conducted an upregulation of SPT from the high metabolic activity in the cells. In conclusion, the viability and gene expression were most likely related to osmotic stress but should be further analyzed with digital holographic microscopy (DHM) and Western blot. 

  • 2. Bergh, A-C
    et al.
    El-Schich, Zahra
    Malmö högskola, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV).
    Delfani, P
    Ohlsson, L
    Rósen, A
    Gjörloff Wingren, A
    B cell receptor signaling suppressor SHP-1 is active in CLL lymph node and peripheral blood2016Manuskript (preprint) (Annet vitenskapelig)
  • 3. Berglund, Mattias
    et al.
    Thunberg, Ulf
    Fridberg, Marie
    Gjörloff Wingren, Anette
    Malmö högskola, Fakulteten för hälsa och samhälle (HS).
    Gullbo, Joachim
    Leuchowius, KJ
    Amini, Ros-Marie
    Lagercrantz, Svetlana
    Horvat, Andrea
    Enblad, Gunilla
    Söderberg, Ola
    Establishment of a cell line from a chemotherapy resistant diffuse large B-cell lymphoma2007Inngår i: Leukemia Lymphoma, Vol. 48, nr 5, s. 1038-1041Artikkel i tidsskrift (Fagfellevurdert)
  • 4. Buhlin, Kåre
    et al.
    Hultin, Margareta
    Norderyd, Ola
    Malmö högskola, Odontologiska fakulteten (OD).
    Persson, Lena
    Pockley, Alan Graham
    Pussinen, Pirkko J.
    Rabe, Per
    Klinge, Björn
    Malmö högskola, Odontologiska fakulteten (OD).
    Gustafsson, Anders
    Periodontal treatment influences risk markers for atherosclerosis in patients with severe periodontitis2009Inngår i: Atherosclerosis, ISSN 0021-9150, E-ISSN 1879-1484, Vol. 206, nr 2, s. 518-522Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    This study investigated the effect of mechanical infection control for periodontitis and periodontal surgery on the prevalence of well-established risk factors for atherosclerosis, and plasma levels of cytokines, antibodies against heat shock proteins and markers of systemic inflammation. Sixty-eight patients between 39 and 73 years of age with severe periodontitis who had been referred to four specialist periodontology clinics in Sweden were investigated. A fasting venous blood sample was taken at baseline and additional samples were collected after 3 and 12 months. A total of 54 patients underwent periodontal treatment. The periodontal treatment was successful, as pathogenic gingival pockets decreased significantly. Plasma glucose, lipids and markers of systemic inflammation were not significantly altered after 3 months. One year after the initial treatment, HDL-C concentrations were significantly increased (Delta0.08mmol/L) whereas LDL-C concentrations decreased (Delta0.23mmol/L). Haptoglobin concentrations were also lower. Interleukin-18 and interferon-gamma levels were also lower after 12 months (60ng/L (-23%) and 11ng/L (-97%) respectively). Treatment had no effect on plasma levels of IgA, IgG1, IgG2 antibodies against heat shock proteins. In conclusion, this study indicates that standard treatment for periodontal disease induces systemic changes in several biochemical markers that reflect the risk for atherosclerosis.

    Fulltekst (pdf)
    FULLTEXT01
  • 5. Buhlin, Kåre
    et al.
    Hultin, Margareta
    Norderyd, Ola
    Malmö högskola, Odontologiska fakulteten (OD).
    Persson, Lena
    Pockley, Alan Graham
    Rabe, Per
    Klinge, Björn
    Malmö högskola, Odontologiska fakulteten (OD).
    Gustafsson, Anders
    Risk factors for atherosclerosis in cases with severe periodontitis2009Inngår i: Journal of Clinical Periodontology, ISSN 0303-6979, E-ISSN 1600-051X, Vol. 36, nr 7, s. 541-549Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    AIM: Studies have reported on an association between cardiovascular disease (CVD) and periodontitis. The purpose of this case-control study was to provide an insight into this association by determining the plasma levels of some risk markers for CVD in cases with periodontitis. MATERIALS AND METHODS: Sixty-eight cases with periodontitis, mean age 53.9 (SD 7.9) years, and 48 randomly selected healthy controls, mean age 53.1 (SD 7.9) years, were investigated. Fasting blood plasma was analysed for glucose, lipids, markers systemic inflammation, cytokines and antibodies against heat shock proteins (Hsp). The associations between periodontitis and the various substances analysed in plasma were calculated using a multivariate logistic regression model, which compensated for age, gender, smoking and body mass index. RESULTS: The regression analyses revealed a significant association between periodontitis and high levels of C-reactive protein (CRP) [odds ratio (OR) 4.0, confidence interval (CI) 1.4-11.4] and fibrinogen (OR 8.7, CI 2.6-28.4), IL-18 (OR 6.5, CI 2.2-19.5), and decreased levels of IL-4 (OR 0.12, CI 0.0-0.5). The study showed increased levels of antibodies against Hsp65 (OR 2.8, CI 1-7.6) and 70 (OR 2.9, CI 1.1-7.8) and decreased levels of antibodies against Hsp60 (OR 0.3, CI 0.1-0.8). CONCLUSIONS: Periodontitis was associated with increased levels of CRP, glucose, fibrinogen and IL-18, and with decreased levels of IL-4.

  • 6.
    Chaves, Roberta Rayra Martins
    et al.
    Department of Oral Surgery and Pathology, School of Dentistry, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, Brazil.
    Guimarães, Letícia Martins
    Department of Oral Surgery and Pathology, School of Dentistry, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, Brazil.
    Pereira, Thaís Dos Santos Fontes
    Department of Oral Surgery and Pathology, School of Dentistry, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, Brazil.
    Pereira, Núbia Braga
    Department of Pathology, Biological Sciences Institute, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, Brazil.
    Chrcanovic, Bruno
    Malmö universitet, Odontologiska fakulteten (OD). Malmö universitet, Biofilms Research Center for Biointerfaces.
    Fonseca, Felipe Paiva
    Department of Oral Surgery and Pathology, School of Dentistry, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, Brazil.
    Lafuente-Ibáñez de Mendoza, Irene
    Unit of Oral and Maxillofacial Pathology of the Dental Clinic Service, Department of Stomatology II, University of the Basque Country (UPV-EHU), Bizkaia, Spain.
    Aguirre-Urizar, José Manuel
    Unit of Oral and Maxillofacial Pathology of the Dental Clinic Service, Department of Stomatology II, University of the Basque Country (UPV-EHU), Bizkaia, Spain.
    Cavaliéri Gomes, Carolina
    Department of Pathology, Biological Sciences Institute, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, Brazil.
    Santiago Gomez, Ricardo
    Department of Oral Surgery and Pathology, School of Dentistry, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, Brazil.
    KRAS mutations in implant‐associated peripheral giant cell granuloma2020Inngår i: Oral Diseases, ISSN 1354-523X, E-ISSN 1601-0825, Vol. 26, nr 2, s. 334-340Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Objectives: To investigate the molecular pathogenesis of implant‐associated peripheral giant cell granuloma (IA‐PGCG). Methods: A convenience sample of 15 IA‐PGCG cases was selected. Hotspot mutations of KRAS, FGFR1, and TRPV4 genes, previously reported in conventional giant cell lesions of the jaws, were investigated by Sanger sequencing. As these mutations could activate MAPK/ERK pathway, the expression of phospho‐ERK1/2 was also evaluated by immunohistochemistry. Results: KRAS mutations were detected in 8/15 (53.4%) samples. Similar to conventional peripheral giant cell granuloma, the KRAS mutations most frequently occurred in codon 146 (p.A146V, n = 3), followed by codon 12 (p.G12A and p.G12D, n = 1 each) and codon 14 (p.V14L, n = 1). Variants of unknown significance (VUS) were also detected in two cases, affecting codons 37 (p.E37K) and 127 (p.T127I). All samples showed wild‐type (WT) sequences for FGFR1 and TRPV4 genes. Consistent with MAPK/ERK pathway activation, all mononuclear cells of the lesion showed strong staining for phospho‐ERK1/2 protein in the immunohistochemical analysis. Conclusions: KRAS mutations and activation of the MAPK‐ERK signaling pathway occur in IA‐PGCG. This is the first study to demonstrate cancer‐associated gene mutations in a non‐neoplastic reactive condition associated with dental implants.

  • 7.
    Correa, Yubexi
    et al.
    Malmö universitet, Biofilms Research Center for Biointerfaces. Malmö universitet, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV).
    Ravel, Mathilde
    Malmö universitet, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV). Malmö universitet, Biofilms Research Center for Biointerfaces.
    Imbert, Marie
    Malmö universitet, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV). Malmö universitet, Biofilms Research Center for Biointerfaces.
    Waldie, Sarah
    Malmö universitet, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV). Malmö universitet, Biofilms Research Center for Biointerfaces.
    Clifton, Luke
    Harwell Sci & Innovat Campus, Sci & Technol Facil Council, Rutherford Appleton Lab, ISIS Pulsed Neutron & Muon Source, Didcot, England..
    Terry, Ann
    Lund Univ, MAX Lab 4, CoSAXS Beamline, Lund, Sweden..
    Roosen-Runge, Felix
    Malmö universitet, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV). Malmö universitet, Biofilms Research Center for Biointerfaces.
    Lagerstedt, Jens O.
    Lund Univ, Diabet Ctr, Dept Clin Sci Malmö, Islet Cell Exocytosis, Malmö, Sweden.;Novo Nordisk, Rare Endocrine Disorders, Res & Early Dev, Copenhagen, Denmark..
    Moir, Michael
    Australian Nucl Sci & Technol Org ANSTO, Natl Deuterat Facil, Lucas Heights, NSW, Australia..
    Darwish, Tamim
    Australian Nucl Sci & Technol Org ANSTO, Natl Deuterat Facil, Lucas Heights, NSW, Australia.;Univ Canberra, Fac Sci & Technol, Canberra, ACT, Australia..
    Cárdenas, Marité
    Malmö universitet, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV). Malmö universitet, Biofilms Research Center for Biointerfaces. Basque Fdn Sci, Ikerbasque, Bilbao, Spain.;Univ Basque Country, Biofis Inst, Leioa, Spain..
    Del Giudice, Rita
    Malmö universitet, Biofilms Research Center for Biointerfaces. Malmö universitet, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV).
    Lipid exchange of apolipoprotein A-I amyloidogenic variants in reconstituted high-density lipoprotein with artificial membranes2024Inngår i: Protein Science, ISSN 0961-8368, E-ISSN 1469-896X, Vol. 33, nr 5, artikkel-id e4987Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    High-density lipoproteins (HDLs) are responsible for removing cholesterol from arterial walls, through a process known as reverse cholesterol transport. The main protein in HDL, apolipoprotein A-I (ApoA-I), is essential to this process, and changes in its sequence significantly alter HDL structure and functions. ApoA-I amyloidogenic variants, associated with a particular hereditary degenerative disease, are particularly effective at facilitating cholesterol removal, thus protecting carriers from cardiovascular disease. Thus, it is conceivable that reconstituted HDL (rHDL) formulations containing ApoA-I proteins with functional/structural features similar to those of amyloidogenic variants hold potential as a promising therapeutic approach. Here we explored the effect of protein cargo and lipid composition on the function of rHDL containing one of the ApoA-I amyloidogenic variants G26R or L174S by Fourier transformed infrared spectroscopy and neutron reflectometry. Moreover, small-angle x-ray scattering uncovered the structural and functional differences between rHDL particles, which could help to comprehend higher cholesterol efflux activity and apparent lower phospholipid (PL) affinity. Our findings indicate distinct trends in lipid exchange (removal vs. deposition) capacities of various rHDL particles, with the rHDL containing the ApoA-I amyloidogenic variants showing a markedly lower ability to remove lipids from artificial membranes compared to the rHDL containing the native protein. This effect strongly depends on the level of PL unsaturation and on the particles' ultrastructure. The study highlights the importance of the protein cargo, along with lipid composition, in shaping rHDL structure, contributing to our understanding of lipid-protein interactions and their behavior.

    Fulltekst (pdf)
    fulltext
  • 8.
    Del Giudice, Rita
    et al.
    Malmö universitet, Biofilms Research Center for Biointerfaces. Malmö universitet, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV). Lund Univ, Dept Expt Med Sci, S-22184 Lund, Sweden..
    Lindvall, Mikaela
    Lund Univ, Dept Expt Med Sci, S-22184 Lund, Sweden..
    Nilsson, Oktawia
    Lund Univ, Dept Expt Med Sci, S-22184 Lund, Sweden..
    Monti, Daria Maria
    Univ Napoli Federico II, Dept Chem Sci, Complesso Univ Monte St Angelo, I-80126 Naples, Italy.;Ist Nazl Biostrutture & Biosistemi INBB, I-00136 Rome, Italy..
    Lagerstedt, Jens O.
    Lund Univ, Dept Expt Med Sci, S-22184 Lund, Sweden.;Lund Univ, Diabet Ctr, Dept Clin Sci Malmö, Islet Cell Exocytosis, S-20506 Malmö, Sweden.;Novo Nord AS, DK-2880 Bagsvaerd, Denmark..
    The Apparent Organ-Specificity of Amyloidogenic ApoA-I Variants Is Linked to Tissue-Specific Extracellular Matrix Components2023Inngår i: International Journal of Molecular Sciences, ISSN 1661-6596, E-ISSN 1422-0067, Vol. 24, nr 1, artikkel-id 318Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Apolipoprotein A-I (ApoA-I) amyloidosis is a rare protein misfolding disease where fibrils of the N-terminal domain of the protein accumulate in several organs, leading to their failure. Although ApoA-I amyloidosis is systemic, the different amyloidogenic variants show a preferential tissue accumulation that appears to correlate with the location of the mutation in the protein sequence and with the local extracellular microenvironment. However, the factors leading to cell/tissues damage, as well as the mechanisms behind the observed organ specificity are mostly unknown. Therefore, we investigated the impact of ApoA-I variants on cell physiology and the mechanisms driving the observed tissue specificity. We focused on four ApoA-I amyloidogenic variants and analyzed their cytotoxicity as well as their ability to alter redox homeostasis in cell lines from different tissues (liver, kidney, heart, skin). Moreover, variant-specific interactions with extracellular matrix (ECM) components were measured by synchrotron radiation circular dichroism and enzyme-linked immunosorbent assay. Data indicated that ApoA-I variants exerted a cytotoxic effect in a time and cell-type-specific manner that seems to be due to protein accumulation in lysosomes. Interestingly, the ApoA-I variants exhibited specific preferential binding to the ECM components, reflecting their tissue accumulation pattern in vivo. While the binding did not to appear to affect protein conformations in solution, extended incubation of the amyloidogenic variants in the presence of different ECM components resulted in different aggregation propensity and aggregation patterns.

    Fulltekst (pdf)
    fulltext
  • 9. Dérand, Per
    et al.
    Warfvinge, Gunnar
    Malmö högskola, Odontologiska fakulteten (OD).
    Thor, Andreas
    Glomangioma. A case presentation2010Inngår i: Journal of oral and maxillofacial surgery (Print), ISSN 0278-2391, E-ISSN 1531-5053, Vol. 68, nr 1, s. 204-207Artikkel i tidsskrift (Fagfellevurdert)
  • 10.
    El-Schich, Zahra
    Malmö universitet, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV). Malmö universitet, Biofilms Research Center for Biointerfaces.
    Digital holographic microscopy: a noninvasive method to analyze the formation of spheroids2021Inngår i: BioTechniques, ISSN 0736-6205, E-ISSN 1940-9818, Vol. 71, nr 6, s. 598-603Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Digital holographic (DH) microscopy is a unique noninvasive method to analyze living cells. With DH microscopy, in vitro cell cultures can be imaged in 2D and pseudo-3D and measurements of size and morphology of the cells are provided. Here, a description of a novel methodology utilizing DH microscopy for the analysis of spheroids is presented. A cell culture protocol is introduced and morphological parameters of cell spheroids as measured by DH microscopy are presented. The study confirms the use of DH microscopy for the analysis of cell spheroids. In the future, organoids can be analyzed with DH microscopy, and it can also be used for drug response and cell death analyses. Method summary This method aims to optimize DH microscopy for the analysis of morphological parameters of spheroids in an easy and convenient way. Cell spheroids were cultured in culture dishes for 5 days. Then, the lid was replaced with HoloLid. After that, the spheroids were imaged and quantitative morphological information was collected and analyzed.

    Fulltekst (pdf)
    fulltext
  • 11. Fridberg, Marie
    et al.
    Servin, Anna
    Anagnostaki, Lola
    Linderoth, Johan
    Berglund, Mattias
    Söderberg, Ola
    Enblad, Gunilla
    Rosén, Anders
    Mustelin, Tomas
    Jerkeman, Mats
    Persson, Jenny
    Gjörloff Wingren, Anette
    Malmö högskola, Fakulteten för hälsa och samhälle (HS).
    Protein expression and cellular localization in two prognostic subgroups of diffuse large B-cell lymphoma: Higher expression of ZAP70 and PKC-beta II in the non-germinal center group and poor survival in patients deficient in nuclear PTEN2007Inngår i: Leukemia and Lymphoma, ISSN 1042-8194, E-ISSN 1029-2403, Vol. 48, nr 11, s. 2221-2232Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Patients diagnosed with diffuse large B-cell lymphoma (DLBCL) show varying responses to conventional therapy, and this might be contributed to the differentiation stage of the tumor B-cells. The aim of the current study was to evaluate a panel of kinases (ZAP70, PKC-beta I and II and phosphorylated PKB/Akt) and phosphatases (PTEN, SHP1 and SHP2) known to be frequently deregulated in lymphoid malignancies. De novo DLBCL cases were divided into two subgroups, the germinal center (GC) group (14/28) and the non-germinal center (non-GC) or activated B-cell (ABC) group (14/28). ZAP70 and PKC-beta II were expressed in a significantly higher percentage of tumor cells in the clinically more aggressive non-GC group compared with the prognostically favourable GC group. Also, the subcellular localization of PKC-beta I and II differed in DLBCL cells, with the PKC-beta I isoform being expressed in both the cytoplasm and nucleus, while PKC-beta II was found exclusively in the cytoplasm. Loss of nuclear PTEN correlated with poor survival in cases from both subgroups. In addition, five cell lines of DLBCL origin were analyzed for protein expression and for mRNA levels of PTEN and SHP1. For the first time, we show that ZAP70 is expressed in a higher percentage of tumor cells in the aggressive non-GC subgroup of DLBCL and that PKC-beta I and II are differently distributed in the two prognostic subgroups of de novo DLBCL.

    Fulltekst (pdf)
    FULLTEXT01
  • 12. Gauffin, Fredrika
    et al.
    Diffner, Eva
    Gustafsson, Bertil
    Nordgren, Ann
    Gjörloff Wingren, Anette
    Malmö högskola, Fakulteten för hälsa och samhälle (HS).
    Sander, Birgitta
    Persson Liao, Jenny
    Gustafsson, Britt
    Expression of PTEN and SHP1, investigated from tissue micro arrays in pediatric acute lymphoblastic leukaemia2009Inngår i: Pediatric Hematology & Oncology, ISSN 0888-0018, E-ISSN 1521-0669, Vol. 26, nr 1, s. 48-56Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    PTEN and SHP1 are tumor suppressor genes involved in the regulation of cell cycle control and apoptosis. We have investigated the protein expression of PTEN and SHP 1, by immunohistochemistry in micro tissue arrays from bone marrow samples in children, diagnosed with acute lymphoblastic leukaemia and non-malignant controls. PTEN was over expressed in ALL samples, while SHP1 showed a low expression. PTEN showed a significant difference in expression compared to non-malignant controls. The role of PTEN and SHP1 are not well investigated in pediatric leukemias and could in future play a role as prognostic factors.

  • 13.
    Gjörloff Wingren, Anette
    et al.
    Malmö universitet, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV). Malmö universitet, Biofilms Research Center for Biointerfaces.
    Ziyad Faik, Riyam
    Malmö universitet, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV). Malmö universitet, Biofilms Research Center for Biointerfaces.
    Holefors, Anna
    In Vitro Plant-Tech AB, Geijersg 4B, 21618 Limhamn, Sweden.
    Filecovic, Edina
    Malmö universitet, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV). Malmö universitet, Biofilms Research Center for Biointerfaces.
    Gustafsson, Anna
    Malmö universitet, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV). Malmö universitet, Biofilms Research Center for Biointerfaces.
    In vitro effects of undifferentiated callus extracts from Plantago major L, Rhodiola rosea L and Silybum marianum L in normal and malignant human skin cells cells2023Inngår i: Heliyon, E-ISSN 2405-8440, Vol. 9, nr 6, artikkel-id e16480Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    BACKGROUND AND OBJECTIVES: L was investigated both in normal and malignant skin cells.

    METHODS: Antioxidant activity of the extracts was analyzed by using the Trolox Equivalent Antioxidant Capacity (TEAC) assay. High-Performance Thin-Layer Chromatography (HPTLC) was performed to demonstrate the phytochemical profile, and the total flavonoid content was analyzed with an aluminum chloride colorimetric method. The anti-inflammatory effect was investigated by cell treatments using the plant extracts. Thereafter, the possible suppression of induced IL-6 response was measured from the cultured skin cancer cell lines A2058 and A431, and normal primary keratinocytes with Enzyme-Linked Immunosorbent Assay (ELISA).

    RESULTS: also had the highest flavonoid content and showed the highest antioxidant activity of the three extracts tested.

    CONCLUSION: possess properties such as antioxidant and anti-inflammatory activities in both normal and malignant keratinocytes, and thus could be a promising agent controlling the pro-inflammatory IL-6 production.

    Fulltekst (pdf)
    fulltext
  • 14. Goncalves, Isabel
    et al.
    Stollenwerk, Maria M
    Lindholm, Marie W
    Dias, Nuno
    Pedro, Luis M
    Fernandes e Fernandes, José
    Moses, Jonatan
    Nordin Fredrikson, Gunilla
    Malmö högskola, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV).
    Nilsson, Jan
    Ares, Mikko PS
    Activator protein-1 in carotid plaques is related to cerebrovascular symptoms and cholesteryl ester content2011Inngår i: Cardiovascular pathology, ISSN 1054-8807, E-ISSN 1879-1336, Vol. 20, nr 1, s. 36-43Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Transcription factor activator protein-1 regulates genes involved in inflammation and repair. The aim of this study was to determine whether transcription factor activator protein-1 activity in carotid plaques is related to symptoms, lipid accumulation, or extracellular matrix composition. Methods: Twenty-eight atherosclerotic carotid plaques were removed by endarterectomy and divided into two groups based on the presence or absence of ipsilateral symptoms (b1 month ago). Activator protein-1 DNA binding activity was assessed, and subunit (c-Jun, JunD, JunB, c-Fos, FosB, Fra-1, Fra-2) protein levels analyzed by immunoblotting. Distribution of c-Jun in plaques was analyzed by immunohistochemistry. Results: Plaques associated with symptoms had increased activator protein-1 activity and increased expression of c-Jun and JunD, as compared to asymptomatic plaques. Fra-1 and Fra-2 were present in equal amounts in both groups, whereas JunB, FosB, and c-Fos were undetectable. Activator protein-1 activity correlated with cholesteryl ester and elastin in plaques and decreased with age. Activator protein-1 activity did not correlate with collagen, calcified tissue, or proteoglycan content. Conclusions: Activator protein-1 is increased in plaques associated with symptoms. The correlation between activator protein-1 and cholesteryl esters suggests that high activator protein-1 is a marker of plaque vulnerability. Activator protein-1 expression can also reflect the activation of repair processes.

  • 15. Gorelick, Sergey
    et al.
    Rahkila, Paavo
    Sagari, A.R. Ananda
    Sajavaara, Timo
    Cheng, Sulin
    Karlsson, Lennart B.
    Malmö högskola, Teknik och samhälle (TS).
    van Kan, Jeroen A.
    Whitlow, Harry J
    Growth of osteoblasts on lithographically modified surfaces2007Inngår i: Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms, ISSN 0168-583X, E-ISSN 1872-9584, Vol. 260, nr 1, s. 130-135Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Here we report about preliminary investigations on developing substrates for culturing osteoblasts, the cells responsible for production of mineralised bone, by lithographically modifying the surfaces of several materials. The proton beam writing system at the National University of Singapore was used to fabricate high aspect ratio structures in PMMA, while two-dimensional low aspect ratio structures were fabricated using conventional electron beam lithography (EBL) and UV lithography (UVL) in SU-8. It was found that oxygen plasma treatment of structured SU-8 surfaces changed the surface layer and significantly improved cell attachment and proliferation. Cells grown on patterned thick PMMA exhibit a remarkable geometry-dependent behaviour.

  • 16.
    Hasterok, Sylwia
    et al.
    Malmö universitet, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV). Malmö universitet, Biofilms Research Center for Biointerfaces.
    Gustafsson, Anna
    Malmö universitet, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV). Malmö universitet, Biofilms Research Center for Biointerfaces.
    Gjörloff Wingren, Anette
    Malmö universitet, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV). Malmö universitet, Biofilms Research Center for Biointerfaces.
    Applications of Tumor Cells in an In Vitro 3D Environment2023Inngår i: Applied Sciences, E-ISSN 2076-3417, Vol. 13, nr 18, s. 10349-10349Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Spherical, multicellular aggregates of tumor cells, or three-dimensional (3D) tumor models, can be grown from established cell lines or dissociated cells from tissues in a serum-free medium containing appropriate growth factors. Air–liquid interfaces (ALIs) represent a 3D approach that mimics and supports the differentiation of respiratory tract and skin 3D models in vitro. Many 3D tumor cell models are cultured in conjunction with supporting cell types, such as fibroblasts, endothelial cells, or immune cells. To further mimic the in vivo situation, several extracellular matrix models are utilized to support tumor cell growth. Scaffolds used for 3D tumor cell culture growth include both natural and synthetic hydrogels. Three-dimensional cell culture experiments in vitro provide more accurate data on cell-to-cell interactions, tumor characteristics, drug discovery, metabolic profiling, stem cell research, and diseases. Moreover, 3D models are important for obtaining reliable precision data on therapeutic candidates in human clinical trials before predicting drug cytotoxicity. This review focuses on the recent literature on three different tissue types of 3D tumor models, i.e., tumors from a colorectal site, prostate, and skin. We will discuss the establishment of 3D tumor cell cultures in vitro and the requirement for additional growth support.

    Fulltekst (pdf)
    fulltext
  • 17.
    Jansson, Henrik
    et al.
    Malmö högskola, Odontologiska fakulteten (OD).
    Bratthall, Douglas
    Söderholm, Gunnar
    Clinical Outcome Observed in Subjects with Recurrent Periodontal Disease Following Local Treatment with 25% Metronidazole Gel.2003Inngår i: Journal of Periodontology, ISSN 0022-3492, E-ISSN 1943-3670, Vol. 74, s. 372-377Artikkel i tidsskrift (Annet vitenskapelig)
    Abstract [en]

    BACKGROUND: The aim of this study was to evaluate the clinical outcome in patients with recurrent periodontal disease following treatment with 25% metronidazole gel. METHODS: Twenty subjects in a maintenance care program but with recurrent periodontal disease participated. Three months after scaling and root planing, a total of 40 sites, 2 in each patient, with probing depth > or = 5 mm were selected. One site randomly selected was treated with metronidazole gel (test) and the other site with a placebo gel (control). Baseline and follow-up measurements included plaque index (PI), gingival index (GI), bleeding on probing (BOP), probing depth (PD), and clinical attachment level (CAL). RESULTS: There were no statistically significant differences in PI, GI, BOP, PD, or CAL between test and control sites. CONCLUSION: This study showed that local treatment with 25% metronidazole gel did not seem to influence the clinical healing in this group of subjects with recurrent periodontal disease.

  • 18.
    Jansson, Henrik
    et al.
    Malmö högskola, Odontologiska fakulteten (OD).
    Hamberg, Kristina
    Malmö högskola, Odontologiska fakulteten (OD).
    Söderholm, Göran
    Bratthall, Gunilla
    The Microbial Outcome Observed with Polymerase Chain Reaction in Subjects with Recurrent Periodontal Disease following local treatment with 25% metronidazole gel2004Inngår i: Swedish Dental Journal, ISSN 0347-9994, Vol. 28, nr 2, s. 67-76Artikkel i tidsskrift (Annet vitenskapelig)
    Abstract [en]

    The aim of this study was to evaluate the microbial outcome in patients with recurrent periodontal disease following treatment with 25% metronidazole gel using the polymerase chain reaction (PCR). Twenty subjects in a maintenance care program but with recurrent periodontal disease participated. Three months after scaling and root planing a total of 40 sites, 2 in each patient, with pocket probing depth of > or = 5 mm were selected. One site randomly selected was treated with 25% metronidazole gel (test) and the other site with a placebo gel (control). A bacterial sample was collected on paperpoint from each test and control site at baseline and 12 weeks after treatment. The following pathogens were analysed and detected with PCR:Actinobacillus actinomycetemcomitans (A.a.), Porphyromonas gingivalis (P.g.) and Prevotella nigrescens (P.n.). At baseline, A.a., P.g. and P.n. were detected in 30, 60 and 70% of all test sites and in 32, 58 and 21% of all control sites. There was a statistically significant difference between the test and control sites for P.n. at baseline. The major difference after treatment with 25% metronidazole gel was the increase of positive control sites for P.g. and P.n. However, there were no statistically significant differences in the occurrence rate of A.a., P.g. and P.n. at test and control sites after treatment. This study has shown that 25% metronidazole gel treatment did not seem to influence the microbial outcome, when PCR was used to analyse the presence/absence of A.a., P.g. and P.n. in this group of subjects with recurrent periodontal disease.

  • 19.
    Jansson, Henrik
    et al.
    Malmö högskola, Odontologiska fakulteten (OD).
    Lyssenko, Valeriya
    Gustavsson, Åsa
    Hamberg, Kristina
    Malmö högskola, Odontologiska fakulteten (OD).
    Söderfeldt, Björn
    Malmö högskola, Odontologiska fakulteten (OD).
    Groop, Leif
    Bratthall, Gunilla
    Analysis of the interleukin-1 and interleukin-6 polymorphisms in patients with chronic periodontitis. A pilot study2006Inngår i: Swedish Dental Journal, ISSN 0347-9994, Vol. 30, nr 1, s. 17-23Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    The aim of this study was to analyse whether the interleukin-1 (IL-1) and IL-6 gene polymorphisms were associated with the susceptibility of chronic periodontitis. Genomic DNA was obtained from 20 patients with chronic periodontitis and 31 periodontally healthy subjects. All subjects were of North European heritage. The test subjects were kept in a maintenance program after periodontal treatment but yet showing signs of recurrent disease. Genotyping of the IL-1alpha [+4845C>T], IL-1beta [-3954C>T] and IL-6 [-174G>C] polymorphisms was carried out using an allelic discrimination Assay-by-Design method on ABI PRISM 7900 Sequence Detection System. All genotypes were analyzed using the GeneMapper 2.0 software. A similar distribution of Single Nucleotide Polymorphism (SNP) was seen in both groups. Analysis by logistic regression including gender, IL-1alpha [+4845C>T], IL-1beta [-3954C>T], IL-6 [-174G>C] genotypes, the composite IL-1 genotype, the combination of the composite IL-1 genotype and the IL-6 -174G>C genotype and adjusting for smoking did not result in any statistically significant difference. SNPs in IL-1alpha [+4845C>T], IL-1beta [-3954C>T] and IL-6 [-174G>C] do not seem to increase the susceptibility to chronic periodontitis in this group of subjects.

  • 20.
    Jansson, Henrik
    et al.
    Malmö högskola, Odontologiska fakulteten (OD).
    Norderyd, Ola
    Malmö högskola, Odontologiska fakulteten (OD).
    Evaluation of a periodontal risk assessment model in subjects with severe periodontitis. A 5-year retrospective study2008Inngår i: Swedish Dental Journal, ISSN 0347-9994, Vol. 32, nr 1, s. 1-7Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    The aim of this study was to evaluate a well-established periodontal risk assessment tool in patients with severe periodontitis included in a supportive periodontal treatment (SPT) program. In total 20 individuals were included in the analysis. All subjects were randomly selected after successful periodontal treatment and at least 5 years SPT. Clinical and radiographic measurements were collected from patient records and analyzed according to the periodontal risk assessment model. Using the periodontal risk assessment model all subjects were classified as low, moderate, or high-risk patients. According to the model 7 patients were classified as moderate risk patients and 13 as high-risk patients. When comparing all the patients using only bleeding on probing (BoP) mean prevalence of 20% as a cut-off point, 15 patients were categorised as having low-moderate risk for periodontitis progression and 5 subjects as having high-risk for disease progression. The periodontal risk assessment model seems to overestimate the risk for disease progression. However the model is a suitable tool to visualize for both the clinician and the patient different variables of importance for periodontal health. The model is also beneficial to show how periodontal treatment can reduce further risk for periodontal disease.

  • 21.
    Jönsson, Daniel
    et al.
    Malmö högskola, Odontologiska fakulteten (OD).
    Nebel, Daniel
    Malmö högskola, Odontologiska fakulteten (OD).
    Bratthall, Gunilla
    Nilsson, Bengt-Olof
    LPS-induced MCP-1 and IL-6 production is not reversed by oestrogen in human periodontal ligament cells2008Inngår i: Archives of Oral Biology, ISSN 0003-9969, E-ISSN 1879-1506, Vol. 58, nr 9, s. 896-902Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Department of Periodontology, Faculty of Odontology, Malmö University, SE-205 06 Malmö, Sweden. daniel.jonsson@od.mah.se OBJECTIVE: Periodontal ligament (PDL) cells express oestrogen receptors but the functional importance of oestrogen in PDL cells exposed to bacterial endotoxins is not known. Here we investigate if the inflammation promoter lipopolysaccharide (LPS) affects PDL cell production of interleukin-6 (IL-6), monocyte chemoattractant protein-1 (MCP-1), C-reactive protein (CRP) and/or normal functional PDL cell characteristics such as collagen synthesis and cell proliferation and if oestrogen modulates the effects of LPS. METHODS: PDL cells were obtained from periodontal ligament of premolars. PDL cells were treated with Escherichia coli LPS in the absence or presence of oestrogen (17beta-oestradiol, E2). Cellular concentration of IL-6, MCP-1 and CRP was determined by enzyme-linked immunosorbent assay (ELISA). Collagen synthesis was determined by l-[3H]proline incorporation. Cell proliferation was assessed by DNA synthesis measurement using [3H]thymidine incorporation. RESULTS: Stimulation with LPS (500 ng/ml to 10 microg/ml) increased IL-6 production in a concentration-dependent manner. Lower concentration (100 ng/ml) of LPS had no effect. LPS-induced stimulation of IL-6 was not reversed by a physiologically high concentration (100 nM) of E2. LPS increased also MCP-1 production which was unaffected by E2. Treatment with E2 alone had no effect on either IL-6 or MCP-1. Stimulation with LPS had no effect on CRP. LPS did not affect collagen synthesis and cell proliferation, reflecting normal physiological properties of PDL cells. CONCLUSIONS: LPS stimulates PDL cell IL-6 and MCP-1 production but has no effect on the normal physiological properties of PDL cells. LPS-induced IL-6 and MCP-1 is not reversed by oestrogen suggesting that oestrogen exerts no anti-inflammatory effect via this mechanism.

  • 22. Karlsson, Marcus R
    et al.
    Diogo Löfgren, Christina
    Malmö högskola, Odontologiska fakulteten (OD).
    Jansson, Henrik
    Malmö högskola, Odontologiska fakulteten (OD).
    The effect of laser therapy as an adjunct to non-surgical periodontal treatment in subjects with chronic periodontitis: a systematic review2008Inngår i: Journal of Periodontology, ISSN 0022-3492, E-ISSN 1943-3670, Vol. 79, nr 11, s. 2021-2028Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Background: The objective of this study was to systematically review the evidence on the effectiveness of laser therapy as an adjunct to non-surgical periodontal treatment in adults with chronic periodontitis. Methods: A search was conducted for randomized controlled trials comparing the outcome of periodontitis with laser as an adjunct to scaling and root planing in the treatment of chronic periodontal disease. The electronic databases, PubMed and Cochrane Central Register of Controlled Trials, were used as data sources. Screening, data abstraction, and quality assessment were conducted independently by three reviewers (MK, HJ, and CDL). The primary outcome measures evaluated were changes in clinical attachment level, probing depth, and bleeding on probing. Results: The search resulted in 25 abstracts; four randomized controlled clinical trials were included. Four different laser methods were used; consequently, it was impossible to conduct a quantitative data synthesis leading to a meta-analysis. All studies included a limited number of subjects. Conclusions: No consistent evidence supports the efficacy of laser treatment as an adjunct to non-surgical periodontal treatment in adults with chronic periodontitis. More randomized controlled clinical trials are needed. PMID: 18980508 [PubMed - in process]

  • 23.
    Lindberg, Pia
    et al.
    Malmö högskola, Odontologiska fakulteten (OD).
    Larsson, Åke
    Malmö högskola, Odontologiska fakulteten (OD).
    Nielsen, Boye Schnack
    Expression of plasminogen activator inhibitor-1, urokinase receptor and laminin gamma-2 chain is an early coordinated event in incipient oral squamous cell carcinoma2006Inngår i: International Journal of Cancer, ISSN 0020-7136, E-ISSN 1097-0215, Vol. 118, nr 12, s. 2948-2956Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    We have studied the expression of plasminogen activator inhibitor-1 (PAI-1) and urokinase receptor (uPAR) together with the gamma2-chain of laminin-5 (lam-gamma2) by immunohistochemistry in 20 cases with incipient oral squamous cell carcinoma (SCC). PAI-1-positive neoplastic cells located at the tip of the putative invasive front of grade 1 (incipient) carcinoma were seen in 16 of the 20 cases (75%), whereas adjacent normal and dysplastic epithelium was PAI-1-negative. Clusters of putative invasive neoplastic cells located in the lamina propria were PAI-1-positive in areas with grade 2 incipient carcinoma as were invasive cancer cells in areas of grade 3-4 invasive carcinoma. uPAR immunoreactivity was strongly expressed in numerous stromal cells in the carcinoma area in all 20 lesions, while a few uPAR-positive stromal cells were found in areas with normal and dysplastic epithelium. uPAR-positive neoplastic cell islands located at the front of the lesions were seen in 15 of the 20 cases. The expression pattern of lam-gamma2 was very similar to that of PAI-1; however, lam-gamma2-positive neoplastic cells were only detected in 11 of the 20 cases (55%) in areas of grade 1 incipient carcinoma. Direct comparison of the 3 components revealed colocalization in neoplastic cell islands in both incipient and invasive SCC. Our results suggest that PAI-1 is a novel potential marker of initial invasion in oral SCC, and that the coordinated expression of PAI-1 with uPAR and lam-gamma2 sustain the features of the early invasive cancer cells.

  • 24.
    Lysén, Martin
    Malmö universitet, Fakulteten för hälsa och samhälle (HS).
    SPHEROID CULTIVATION: AND MOTILITY REDUCTION THROUGH ACE2-RBDINTERACTION OF MONOLAYER COUNTERPARTS2021Independent thesis Basic level (degree of Bachelor), 10 poäng / 15 hpOppgave
    Abstract [en]

    Spheroids are three-dimensional (3D) cell structures of aggregated cells that more closely mimic the cell-cell interactions and microenvironment of tumors. In this study 0,1% v/v of nano fibrillar cellulose hydrogel solubilized in spheroid medium was tested against static suspension culture. The aim was to determine whether either of the mediums was suitable to make the cell lines MCF-7, MDAMB-231, HMEC-1 as well as co-cultures of MCF-7/HMEC-1 and MDA-MB-231to form growth as spheroids. This study used holographic digital microscopy as well as light microscopy with image processing to analyze the cells. This study also examined the possible effect that the receptor-binding domain (RBD) of SARSCov2 might have on cell motility by interacting with angiotensin-converting enzyme 2 (ACE2). This study also included cross-linking of the RBD-ACE2complex to strengthen the RBD-ACE2 interaction. To this end, a motility assay was conducted on monolayers of the cell lines. This study found that among the cell lines and co-cultures only MCF-7 and MCF-7/HMEC-1 formed spheroids. The other cell lines and co-culture formed loose or tight aggregates. This study also showed that there was a significant difference in motility between the cell lines and indicated upon the difference between cells treated these results implicate decreased invasiveness by tumors in cancer patients due to toRBD-ACE2 interactions. This merits further experiments to determine whether-ACE2 treatment in vitro can serve to decrease the motility of tumor cells in cultures. 

  • 25.
    Miskelly, Michael G
    et al.
    Neuroendocrine Cell Biology, Lund University Diabetes Centre, Lund University, Malmö, Sweden.
    Lindqvist, Andreas
    Neuroendocrine Cell Biology, Lund University Diabetes Centre, Lund University, Malmö, Sweden.
    Piccinin, Elena
    Department of Translational Biomedicine and Neuroscience, University of Bari 'Aldo Moro', Bari, Italy; Department of Interdisciplinary Medicine, University of Bari 'Aldo Moro', Bari, Italy.
    Hamilton, Alexander
    Molecular Metabolism, Lund University Diabetes Centre, Lund University, Malmö, Sweden; Islet Cell Exocytosis, Lund University Diabetes Centre, Lund University, Malmö, Sweden.
    Cowan, Elaine
    Islet Cell Exocytosis, Lund University Diabetes Centre, Lund University, Malmö, Sweden.
    Nergård, Bent-Johnny
    Aleris Obesitas, Lund, Sweden.
    Del Giudice, Rita
    Malmö universitet, Biofilms Research Center for Biointerfaces. Malmö universitet, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV). Department of Experimental Medical Science, Lund University, Lund, Sweden.
    Ngara, Mtakai
    Neuroendocrine Cell Biology, Lund University Diabetes Centre, Lund University, Malmö, Sweden.
    Cataldo, Luis R
    Molecular Metabolism, Lund University Diabetes Centre, Lund University, Malmö, Sweden; Novo Nordisk Foundation Centre for Basic Metabolic Research, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.
    Kryvokhyzha, Dmytro
    Bioinformatics Unit, Lund University Diabetes Centre, Lund University, Malmö, Sweden.
    Volkov, Petr
    Bioinformatics Unit, Lund University Diabetes Centre, Lund University, Malmö, Sweden.
    Engelking, Luke
    Internal Medicine, University of Texas Southwestern Medical Center, Dallas, TX, USA; Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, TX, USA.
    Artner, Isabella
    Endocrine Cell Differentiation and Function, Stem Cell Centre, Lund University, Malmö, Sweden.
    Lagerstedt, Jens O
    Islet Cell Exocytosis, Lund University Diabetes Centre, Lund University, Malmö, Sweden; Department of Experimental Medical Science, Lund University, Lund, Sweden.
    Eliasson, Lena
    Islet Cell Exocytosis, Lund University Diabetes Centre, Lund University, Malmö, Sweden.
    Ahlqvist, Emma
    Genomics, Diabetes and Endocrinology, Lund University Diabetes Centre, Lund University, Malmö, Sweden.
    Moschetta, Antonio
    Department of Interdisciplinary Medicine, University of Bari 'Aldo Moro', Bari, Italy; INBB National Institute for Biostructure and Biosystems, Rome, Italy.
    Hedenbro, Jan
    Department of Surgery, Department of Clinical Sciences Lund, Lund University, Lund, Sweden.
    Wierup, Nils
    Neuroendocrine Cell Biology, Lund University Diabetes Centre, Lund University, Malmö, Sweden.
    RNA sequencing unravels novel L cell constituents and mechanisms of GLP-1 secretion in human gastric bypass-operated intestine2024Inngår i: Diabetologia, ISSN 0012-186X, E-ISSN 1432-0428, Vol. 67, nr 2, s. 356-370Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Aims/hypothesis: Roux-en-Y gastric bypass surgery (RYGB) frequently results in remission of type 2 diabetes as well as exaggerated secretion of glucagon-like peptide-1 (GLP-1). Here, we assessed RYGB-induced transcriptomic alterations in the small intestine and investigated how they were related to the regulation of GLP-1 production and secretion in vitro and in vivo.

    Methods: Human jejunal samples taken perisurgically and 1 year post RYGB (n=13) were analysed by RNA-seq. Guided by bioinformatics analysis we targeted four genes involved in cholesterol biosynthesis, which we confirmed to be expressed in human L cells, for potential involvement in GLP-1 regulation using siRNAs in GLUTag and STC-1 cells. Gene expression analyses, GLP-1 secretion measurements, intracellular calcium imaging and RNA-seq were performed in vitro. OGTTs were performed in C57BL/6j and iScd1-/- mice and immunohistochemistry and gene expression analyses were performed ex vivo.

    Results: Gene Ontology (GO) analysis identified cholesterol biosynthesis as being most affected by RYGB. Silencing or chemical inhibition of stearoyl-CoA desaturase 1 (SCD1), a key enzyme in the synthesis of monounsaturated fatty acids, was found to reduce Gcg expression and secretion of GLP-1 by GLUTag and STC-1 cells. Scd1 knockdown also reduced intracellular Ca2+ signalling and membrane depolarisation. Furthermore, Scd1 mRNA expression was found to be regulated by NEFAs but not glucose. RNA-seq of SCD1 inhibitor-treated GLUTag cells identified altered expression of genes implicated in ATP generation and glycolysis. Finally, gene expression and immunohistochemical analysis of the jejunum of the intestine-specific Scd1 knockout mouse model, iScd1-/-, revealed a twofold higher L cell density and a twofold increase in Gcg mRNA expression.

    Conclusions/interpretation: RYGB caused robust alterations in the jejunal transcriptome, with genes involved in cholesterol biosynthesis being most affected. Our data highlight SCD as an RYGB-regulated L cell constituent that regulates the production and secretion of GLP-1.

    Fulltekst (pdf)
    fulltext
  • 26. Mårtensson, Carina
    et al.
    Söderfeldt, Björn
    Malmö högskola, Odontologiska fakulteten (OD).
    Andersson, Pia
    Halling, Arne
    Renvert, Srefan
    Factors behind change in knowledge after a mass media campaign targeting periodontitis2006Inngår i: International Journal of Dental Hygiene, ISSN 1601-5029, E-ISSN 1601-5037, Vol. 4, nr 1, s. 8-14Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    The aim of this study was to investigate changes in knowledge before and after a mass media campaign, in relation to social attributes, care system attributes and oral health aspects. The study was based on a questionnaire in a cohort design, sent out to 900 randomly sampled people aged 50–75 in Sweden. The response rate to the questionnaire before and after the campaign was 70% and 65% respectively. Sixty-four percent answered both questionnaires. Two questions addressed knowledge, while 10 questions aimed to measure social attributes, care system attributes and oral health aspects. Data were analysed for bivariate relations as to change in knowledge and social attributes, care system attributes and oral health aspects. Data were also analysed in multiple regression analysis with knowledge before, knowledge after and knowledge differences as dependent variables. The results showed that there were a number of independent variables with influence on the dependent variables. Of the social attributes, secondary education gave almost 10% (P< 0.001) better knowledge both before and after the campaign. Among care system attributes, high care utilization was related to knowledge both before and after the campaign. The most important factors for knowledge about periodontitis were education, care utilization and perceived importance of oral health. In conclusion, this study demonstrates that mass media might increase knowledge about periodontitis as a health promotion strategy.

  • 27. Mårtensson, Carina
    et al.
    Söderfeldt, Björn
    Malmö högskola, Odontologiska fakulteten (OD).
    Halling, Arne
    Renvert, Stefan
    Knowledge on periodontal disease before and after a mass media campaign2004Inngår i: Swedish Dental Journal, ISSN 0347-9994, Vol. 28, nr 4, s. 165-171Artikkel i tidsskrift (Annet vitenskapelig)
    Abstract [en]

    The aim of this study was to evaluate if a mass media campaign regarding periodontal disease could increase the knowledge in the general population of diagnoses, symptoms and treatment options of periodontal disease. More specifically, the aim was to investigate the number of correct answers to knowledge questions before and after the campaign. The Swedish Association of Periodontology conducted the campaign through brochures, newspapers, radio and TV. The effect of the campaign was evaluated by a pre- and post campaign questionnaire with a cohort design. From a national population register of 50-75 year olds in Sweden, 900 persons were randomly sampled for the study. A total of 64% of the sample answered both questionnaires. The result of the study showed an improvement among the respondents. There was a significant increase in the number of correct answers regarding diagnoses, symptoms and treatments of periodontitis. In the questionnaire, correct answers regarding "Mobile teeth" increased from 57% to 65% (p=0.003) and "careful dental hygiene" from 65% to 73% (p=0.001). Kappa value's were calculated for consistency in the reply and all kappa values were low especially for the questions "X-ray" (0.36) and "Cleaning between the teeth" (0.38). It was concluded that the campaign probably was successful from a public health knowledge standpoint.

  • 28.
    Nebel, Daniel
    et al.
    Malmö högskola, Odontologiska fakulteten (OD).
    Bratthall, Gunilla
    Warfvinge, Gunnar
    Malmö högskola, Odontologiska fakulteten (OD).
    Nilsson, Bengt-Olof
    Effects of ovariectomy and aging on tooth attachment in female mice assessed by morphometric analysis2009Inngår i: Acta Odontologica Scandinavica, ISSN 0001-6357, E-ISSN 1502-3850, Vol. 67, nr 1, s. 8-12Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Objective. Non-human primates, dogs, rats, hamsters and ferrets, have frequently been used as laboratory animals in periodontal biology and pathology. In the past, mice have been used less for this purpose, but nowadays attract a lot of interest because gene knockout and transgenic technologies utilize mice primarily. In this study, we investigate the effects of ovariectomy and aging on tooth attachment in female mice. Material and methods. Eight-week-old mice (n=15) were divided into three experimental groups (control, n=5; sham-operated, n=5; ovariectomy, n=5) and ovaries removed bilaterally. Attachment level, assessed by measuring alveolar bone height and apical termination of the junctional epithelium, was determined 6 weeks post-ovariectomy by digital morphometric analysis in sagittal sections of the mandible. The plasma level of the inflammation marker serum amyloid A (SAA) was determined by ELISA. In another series of experiments, tooth attachment was determined in female mice (n=7) at 8–26 weeks of age. Results. Withdrawal of female sex hormone production by ovariectomy had no effect on alveolar bone height and apical termination of the junctional epithelium. The SAA level in plasma was unaffected by removal of the ovaries, suggesting that systemic inflammation is not induced by ovariectomy. Bone height was similar in mice sacrificed at 8–26 weeks of age and apical termination of the junctional epithelium was at the cemento-enamel junction at all ages. Conclusions. Removal of ovarian production of female sex hormones by ovariectomy has no influence on tooth attachment, and further tooth attachment is preserved with age in female mice.

    Fulltekst (pdf)
    FULLTEXT01
  • 29.
    Nebel, Daniel
    et al.
    Malmö högskola, Odontologiska fakulteten (OD).
    Jonsson, Daniel
    Bratthall, Gunilla
    Nilsson, Bengt-Olof
    Estrogen affects gene expression in LPS stimulated PDL-cells2009Inngår i: Journal of Clinical Periodontology, ISSN 0303-6979, E-ISSN 1600-051X, Vol. 36, nr suppl 9, s. 61-61, artikkel-id 82Artikkel i tidsskrift (Annet vitenskapelig)
    Abstract [en]

    Background: The periodontal ligament cells (PDL cells) play a key role in the formation of the periodontal ligament but these cells have other functions as well. The PDL cells express estrogen receptors but the functional importance is not known. Estrogen modulates inflammation and our hypothesis is that estrogen protects the periodontium via an anti-inflammatory effect on PDL cells. Aim: To identify genes regulated by estrogen in LPS-treated human PDL cells by whole genome arrays. Materials and methods: PDL cells were obtained from human premolars extracted for orthodontic reasons. The cells were divided into two groups. One group was pre-treated with 17β-estradiol (E2, 100 nM) for 2 h and then with Escherichia coli LPS (500ng/ml) for 24 h. The other group was treated with LPS only. Total RNA was extracted and purified by RNeasy Mini Kit (Qiagen®, USA). A whole genome microarray was performed (Affymetrix®, USA) comparing gene expression in the two groups. The cut-off limit was set to a twofold change. Results and Conclusion: Estrogen caused an up-regulation of 38 genes, while 28 genes were down-regulated. Estrogen regulated genes associated with cell-metabolism and cell-signalling but also genes associated with early the inflammatory response. The functional significance of these findings is now determined by e.g. measuring protein levels with ELISA. We conclude that estrogen regulates gene expression in human PDL cells exposed to LPS.

  • 30.
    Nebel, Daniel
    et al.
    Malmö högskola, Odontologiska fakulteten (OD).
    Jönsson, Daniel
    Malmö högskola, Odontologiska fakulteten (OD).
    Bratthall, Gunilla
    Nilsson, Bengt-Olof
    Genes regulated by estrogen in human PDL cells by whole genome arrays2009Konferansepaper (Annet vitenskapelig)
    Abstract [en]

    Background: The periodontal ligament cells (PDL cells) play a key role in the formation of the periodontal ligament but these cells have other functions as well. The PDL cells express estrogen receptors but the functional importance is not known. Estrogen modulates inflammation and our hypothesis is that estrogen protects the periodontium via an anti-inflammatory effect on PDL cells. Aim: To identify genes regulated by estrogen in LPS-treated human PDL cells by whole genome arrays. Materials and methods: PDL cells were obtained from human premolars extracted for orthodontic reasons. The cells were divided into two groups. One group was pre-treated with 17ÿ-estradiol (E2, 100 nM) for 2 h and then with Escherichia coli LPS (500ng/ml) for 24 h. The other group was treated with LPS only. Total RNA was extracted and purified by RNeasy Mini Kit (Qiagen®, USA). A whole genome microarray was performed (Affymetrix®, USA) comparing gene expression in the two groups. The cut-off limit was set to a twofold change. Results and Conclusion: Estrogen caused an up-regulation of 38 genes, while 28 genes were down-regulated. Estrogen regulated genes associated with cell-metabolism and cell-signalling but also genes associated with early the inflammatory response. The functional significance of these findings is now determined by e.g. measuring protein levels with ELISA. We conclude that estrogen regulates gene expression in human PDL cells exposed to LPS.

  • 31.
    Neilands, Jessica
    et al.
    Malmö universitet, Odontologiska fakulteten (OD).
    Svensäter, Gunnel
    Malmö universitet, Odontologiska fakulteten (OD).
    Boisen, Gabriella
    Malmö universitet, Odontologiska fakulteten (OD).
    Robertsson, Carolina
    Malmö universitet, Odontologiska fakulteten (OD).
    Wickström, Claes
    Malmö universitet, Odontologiska fakulteten (OD).
    Davies, Julia R
    Malmö universitet, Odontologiska fakulteten (OD).
    Formation and Analysis of Mono-species and Polymicrobial Oral Biofilms in Flow-Cell Models2023Inngår i: Bacterial Pathogenesis: Methods and Protocols,, Springer, 2023, s. 33-52Kapittel i bok, del av antologi (Fagfellevurdert)
    Abstract [en]

    The oral microbiota, which is known to include at least 600 different bacterial species, is found on the teethand mucosal surfaces as multi-species communities or biofilms. The oral surfaces are covered with a pellicleof proteins absorbed from saliva, and biofilm formation is initiated when primary colonizers, which expresssurface adhesins that bind to specific salivary components, attach to the oral tissues. Further developmentthen proceeds through co-aggregation of additional species. Over time, the composition of oral biofilms,which varies between different sites throughout the oral cavity, is determined by a combination ofenvironmental factors such as the properties of the underlying surface, nutrient availability and oxygenlevels, and bacterial interactions within the community. A complex equilibrium between biofilm communities and the host is responsible for the maintenance of a healthy biofilm phenotype (eubiosis). In the faceof sustained environmental perturbation, however, biofilm homeostasis can break down giving rise todysbiosis, which is associated with the development of oral diseases such as caries and periodontitis.In vitro models have an important part to play in increasing our understanding of the complex processesinvolved in biofilm development in oral health and disease, and the requirements for experimental system,microbial complexity, and analysis techniques will necessarily vary depending on the question posed. In thischapter we describe some current and well-established methods used in our laboratory for studying oralbacteria in biofilm models which can be adapted to suit the needs of individual users. 

  • 32.
    Nilsson, Oktawia
    et al.
    Lund Univ, Dept Expt Med Sci, Lund, Sweden..
    Lindvall, Mikaela
    Lund Univ, Dept Expt Med Sci, Lund, Sweden..
    Obici, Laura
    Fdn IRCCS Policlin San Matteo, Amyloidosis Res & Treatment Ctr, Pavia, Italy..
    Ekstrom, Simon
    Lund Univ, BioMS Swedish Natl Infrastruct Biol Mass Spectrom, Lund, Sweden..
    Lagerstedt, Jens O.
    Lund Univ, Dept Expt Med Sci, Lund, Sweden.;Lund Inst Adv Neutron & Xray Sci LINXS, Lund, Sweden..
    Del Giudice, Rita
    Malmö universitet, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV). Lund Univ, Dept Expt Med Sci, Lund, Sweden.
    Structure dynamics of ApoA-I amyloidogenic variants in small HDL increase their ability to mediate cholesterol efflux2021Inngår i: Journal of Lipid Research, ISSN 0022-2275, E-ISSN 1539-7262, Vol. 62, artikkel-id 100004Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Apolipoprotein A-I (ApoA-I) of high density lipoproteins (HDLs) is essential for the transportation of cholesterol between peripheral tissues and the liver. However, specific mutations in ApoA-I of HDLs are responsible for a late-onset systemic amyloidosis, the pathological accumulation of protein fibrils in tissues and organs. Carriers of these mutations do not exhibit increased cardiovascular disease risk despite displaying reduced levels of ApoA-I/HDL cholesterol. To explain this paradox, we show that the HDL particle profiles of patients carrying either L75P or L174S ApoA-I amyloidogenic variants show a higher relative abundance of the 8.4-nm versus 9.6-nm particles and that serum from patients, as well as reconstituted 8.4- and 9.6-nm HDL particles (rHDL), possess increased capacity to catalyze cholesterol efflux from macrophages. Synchrotron radiation circular dichroism and hydrogen-deuterium exchange revealed that the variants in 8.4-nm rHDL have altered secondary structure composition and display a more flexible binding to lipids than their native counterpart. The reduced HDL cholesterol levels of patients carrying ApoA-I amyloidogenic variants are thus balanced by higher proportion of small, dense HDL particles, and better cholesterol efflux due to altered, region-specific protein structure dynamics.

    Fulltekst (pdf)
    fulltext
  • 33. Nordman, Henrik
    et al.
    Davies, Julia
    Malmö högskola, Odontologiska fakulteten (OD).
    Lindell, Gert
    De Bolos, Carmé
    Real, Francisco
    Carlstedt, Ingemar
    Gastric MUC5AC and MUC6 are large oligomeric mucins which differ in size, glycosylation and tissue distribution2002Inngår i: Biochemical Journal, ISSN 0264-6021, E-ISSN 1470-8728, Vol. 364, s. 191-200Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Gastric MUC5AC and MUC6 mucins were studied using polyclonal antibodies. Immunohistochemistry showed MUC5AC to originate from the surface epithelium, whereas MUC6 was produced by the glands. Mucins from the surface epithelium or glands of corpus and antrum were purified using CsCl/4M guanidinium chloride density-gradient centrifugation. MUC5AC appeared as two distinct populations at 1.4 and 1.3 g/ml, whereas MUC6, which was enriched in the gland tissue, appeared at 1.45 g/ml. Reactivity with antibodies against the Le(b) structure (where Le represents the Lewis antigen) followed the MUC5AC distribution, whereas antibodies against the Le(y) structure and reactivity with the GlcNAc-selective Solanum tuberosum lectin coincided with MUC6, suggesting that the two mucins are glycosylated differently. Rate-zonal centrifugation of whole mucins and reduced subunits showed that both gastric MUC5AC and MUC6 are oligomeric glycoproteins composed of disulphide-bond linked subunits and that oligomeric MUC5AC was apparently smaller than MUC6. A heterogeneous population of 'low-density' MUC5AC mucins, which were smaller than the 'high-density' ones both before and after reduction, reacted with an antibody against a variable number tandem repeat sequence within MUC5AC, suggesting that they represent precursor forms of this mucin. Following ion-exchange HPLC, both MUC5AC and MUC6 appeared as several distinct populations, probably corresponding to 'glycoforms' of the mucins, the most highly charged of which were found in the gland tissue.

  • 34.
    Patel, Megha
    et al.
    Malmö universitet, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV).
    Feith, Marek
    Masaryk Univ, Fac Med, Dept Physiol, Brno 62500, Czech Republic..
    Janicke, Birgit
    Phase Holog Imaging AB, S-22363 Lund, Sweden..
    Alm, Kersti
    Phase Holog Imaging AB, S-22363 Lund, Sweden..
    El-Schich, Zahra
    Malmö universitet, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV). Malmö universitet, Biofilms Research Center for Biointerfaces.
    Evaluation of the Impact of Imprinted Polymer Particles on Morphology and Motility of Breast Cancer Cells by Using Digital Holographic Cytometry2020Inngår i: Applied Sciences, E-ISSN 2076-3417, Vol. 10, nr 3, artikkel-id 750Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Breast cancer is the second most common cancer type worldwide and breast cancer metastasis accounts for the majority of breast cancer-related deaths. Tumour cells produce increased levels of sialic acid (SA) that terminates the monosaccharide on glycan chains of the glycosylated proteins. SA can contribute to cellular recognition, cancer invasiveness and increase the metastatic potential of cancer cells. SA-templated molecularly imprinted polymers (MIPs) have been proposed as promising reporters for specific targeting of cancer cells when deployed in nanoparticle format. The sialic acid-molecularly imprinted polymers (SA-MIPs), which use SA for the generation of binding sites through which the nanoparticles can target and stain breast cancer cells, opens new strategies for efficient diagnostic tools. This study aims at monitoring the effects of SA-MIPs on morphology and motility of the epithelial type MCF-7 and the highly metastatic MDAMB231 breast cancer cell lines, using digital holographic cytometry (DHC). DHC is a label-free technique that is used in cell morphology studies of e.g., cell volume, area and thickness as well as in motility studies. Here, we show that MCF-7 cells move slower than MDAMB231 cells. We also show that SA-MIPs have an effect on cell morphology, motility and viability of both cell lines. In conclusion, by using DH microscopy, we could detect SA-MIPs impact on different breast cancer cells regarding morphology and motility.

    Fulltekst (pdf)
    fulltext
  • 35.
    Robertsson, Carolina
    Malmö universitet, Odontologiska fakulteten (OD). Malmö universitet, Biofilms Research Center for Biointerfaces.
    Responses to External Cues in Oral Bacteria2023Doktoravhandling, med artikler (Annet vitenskapelig)
    Abstract [en]

    This thesis investigates responses to external cues in oral bacteria on a molecular level. Paper I maps Ser/Thr/Tyr phosphorylated proteins in relation to the general proteome in an oral commensal streptococcus (Streptococcus gordonii DL1). The identified phosphoproteins were involved in various bacterial processes, several associated to dysbiosis and development of biofilm-induced disease. Comparison against phosphoproteomes of other bacteria showed many similarities. This is of interest for the identification of shared phosphorylation profiles. 

    Paper II studies differences between the S. gordonii DL1 general proteomes in planktonic and biofilm growth phases, and the regulatory effects of salivary mucin MUC5B on protein expression in the biofilm cells. Regulations in protein expression between the different growth conditions provides insights in bacterial mechanisms for adaptation to the biofilm lifestyle. 

    Paper III examines the regulatory roles of salivary MUC5B on biofilm attachment and metabolic output in two clinical isolates of oral commensals, S. gordonii CW and Actinomyces naeslundii CW. S. gordonii facilitated adhesion of A. naeslundii to MUC5B during early attachment. Both bacteria were also able to utilize MUC5B as a sole nutrient source during early biofilm formation, individually and synergistically in a dual species biofilm. The specific responses elicited by MUC5B in paper II-III seem to promote commensal colonization while down-regulating dysbiosis-related biofilm activities. 

    Microbiological studies are often focused on dysbiosis and development of disease. However, mechanisms that promote eubiosis are equally important to understand how health can be maintained. Findings associated with responses to external cues in oral bacteria may contribute to future development of novel preventative strategies and identification of predictive biomarkers for oral health. 

    Fulltekst (pdf)
    fulltext
    Download (jpg)
    presentationsbild
  • 36.
    Roos‐Jansåker, Ann‐Marie
    Malmö högskola, Odontologiska fakulteten (OD).
    Long time follow up of implant therapy and treatment of peri-implantitis2007Doktoravhandling, med artikler (Annet vitenskapelig)
    Abstract [sv]

    POPULÄRVETENSKAPLIG SAMMANSTÄLLNINGDentala implantat har blivit ett ofta använt alternativ för att ersätta förlorade tänder, vilket resulterat i att en ökad andel av den vuxna befolkningen är försedd med implantatförankrad protetik. Trots attfördelaktiga långtidsresultat av implantatbehandling rapporteratsförekommer infektioner. Hitintills har endast ett fåtal studier inkluderat data om infektioner runt implantat, vilket troligen lett till att denna komplikation vid implantatbehandling underskattats. Det ärmöjligt att vissa infektioner runt implantat utvecklas långsamt och att peri-implantit (infektion runt implantat med benförlust) blir en vanlig komplikation vid implantatbehandling, när fler patienter harhaft sina implantat en lång tid (>10 år). Det finns begränsad information om hur peri-implantit ska behandlas.Målet med avhandlingen var att studera frekvens av implantatförlustersamt förekomst av infektioner runt implantaten i en grupp av patienter som fått Brånemark-implantat installerade för 9-14 årsedan, samt att relatera dessa komplikationer till patient- och implantatspecifika faktorer. Vidare utvärderades tre kirurgiska behandlingsmodeller för peri-implantit.Denna avhandling baseras på sex studier;Studie I-III inkluderar 218 patienter och 1057 implantat som följdes i 9-14 år och utvärderar förekomsten av samt faktorer som kan relateras till implantatförlust och förekomst av samt faktorer relateradetill lesioner runt implantat.11Studie IV är en översiktsartikel som beskriver behandling av infektionerrunt implantat. Studie V är en prospektiv kohortstudie som inkluderar 36 patienteroch 65 implantat och som utvärderar användandet av benersättningsmedelmed eller utan resorberbart membran. Studie VI är en fallstudie med 12 patienter och 16 implantat och som utvärderar benersättningsmedel i kombination med resorberbart membran och täckt läkning.Denna avhandling visar att;Efter 9-14 år finns de flesta implantaten kvar i munnen hos patienterna (95.7%). Patienter som förlorat ett implantat förlorar ofta flera. Implantatförlust är relaterat till förekomst av parodontit (röntgenologisk benförlust på >30 % av tänderna).(Studie I)Peri-implantit är en vanlig klinisk företeelse efter 9-14 år.(Studie II)Vid användandet av implantatet som statistisk enhet förklarasen bennivå på ≥3 gängor (1.8 mm) av förekomst av keratiniseradmukosa och pus. På patientnivå förklaras peri-implantit av parodontit och rökning. (Studie III)Djurstudier har visat att re-osseointgration är möjlig. Majoriteten av humanstudierna är fallstudier. Täckt läkning och bentransplantat kan ge benfyllnad i defekter runt implantat.(Studie IV)Kirurgisk behandling av peri-implantit med ett benersättningsmedel eller benersättningsmedel och ettresorberbart membran resulterade i jämförbara kliniska ochröntgenologiska förbättringar. (Studie V)Benersättningsmedel i kombination med resorberbart membran och täckt läkning resulterade i defektfyllnad med

  • 37.
    Shanbhag, S.
    et al.
    Center for Translational Oral Research (TOR), Department of Clinical Dentistry, Faculty of Medicine, University of Bergen, Bergen, Norway; Department of Immunology and Transfusion Medicine, Haukeland University Hospital, Bergen, Norway.
    Kampleitner, C.
    Ludwig Boltzmann Institute for Traumatology, The Research Center in Cooperation With AUVA, Vienna, Austria; Karl Donath Laboratory for Hard Tissue and Biomaterial Research, University Clinic of Dentistry, Medical University of Vienna, Vienna, Austria; Austrian Cluster for Tissue Regeneration, Vienna, Austria.
    Mohamed-Ahmed, S.
    Center for Translational Oral Research (TOR), Department of Clinical Dentistry, Faculty of Medicine, University of Bergen, Bergen, Norway.
    Yassin, M. A.
    Center for Translational Oral Research (TOR), Department of Clinical Dentistry, Faculty of Medicine, University of Bergen, Bergen, Norway.
    Dongre, H.
    Gade Laboratory for Pathology, Department of Clinical Medicine, Faculty of Medicine, University of Bergen, Bergen, Norway; Centre for Cancer Biomarkers (CCBIO), Faculty of Medicine, University of Bergen, Bergen, Norway.
    Costea, D. E.
    Gade Laboratory for Pathology, Department of Clinical Medicine, Faculty of Medicine, University of Bergen, Bergen, Norway; Centre for Cancer Biomarkers (CCBIO), Faculty of Medicine, University of Bergen, Bergen, Norway.
    Tangl, S.
    Karl Donath Laboratory for Hard Tissue and Biomaterial Research, University Clinic of Dentistry, Medical University of Vienna, Vienna, Austria; Austrian Cluster for Tissue Regeneration, Vienna, Austria.
    Stavropoulos, Andreas
    Malmö universitet, Odontologiska fakulteten (OD). Division of Conservative Dentistry and Periodontology, University Clinic of Dentistry, Medical University of Vienna, Vienna, Austria.
    Bolstad, A. I.
    Center for Translational Oral Research (TOR), Department of Clinical Dentistry, Faculty of Medicine, University of Bergen, Bergen, Norway.
    Suliman, S.
    Center for Translational Oral Research (TOR), Department of Clinical Dentistry, Faculty of Medicine, University of Bergen, Bergen, Norway.
    Mustafa, K.
    Center for Translational Oral Research (TOR), Department of Clinical Dentistry, Faculty of Medicine, University of Bergen, Bergen, Norway.
    Ectopic Bone Tissue Engineering in Mice Using Human Gingiva or Bone Marrow-Derived Stromal/Progenitor Cells in Scaffold-Hydrogel Constructs2021Inngår i: Frontiers in Bioengineering and Biotechnology, E-ISSN 2296-4185, Vol. 9, artikkel-id 783468Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Three-dimensional (3D) spheroid culture can promote the osteogenic differentiation and bone regeneration capacity of mesenchymal stromal cells (MSC). Gingiva-derived progenitor cells (GPC) represent a less invasive alternative to bone marrow MSC (BMSC) for clinical applications. The aim of this study was to test the in vivo bone forming potential of human GPC and BMSC cultured as 3D spheroids or dissociated cells (2D). 2D and 3D cells encapsulated in constructs of human platelet lysate hydrogels (HPLG) and 3D-printed poly (L-lactide-co-trimethylene carbonate) scaffolds (HPLG-PLATMC) were implanted subcutaneously in nude mice; cell-free HPLG-PLATMC constructs served as a control. Mineralization was assessed using micro-computed tomography (µCT), histology, scanning electron microscopy (SEM) and in situ hybridization (ISH). After 4–8 weeks, µCT revealed greater mineralization in 3D-BMSC vs. 2D-BMSC and 3D-GPC (p < 0.05), and a similar trend in 2D-GPC vs. 2D-BMSC (p > 0.05). After 8 weeks, greater mineralization was observed in cell-free constructs vs. all 2D- and 3D-cell groups (p < 0.05). Histology and SEM revealed an irregular but similar mineralization pattern in all groups. ISH revealed similar numbers of 2D and 3D BMSC/GPC within and/or surrounding the mineralized areas. In summary, spheroid culture promoted ectopic mineralization in constructs of BMSC, while constructs of dissociated GPC and BMSC performed similarly. The combination of HPLG and PLATMC represents a promising scaffold for bone tissue engineering applications. 

    Fulltekst (pdf)
    fulltext
  • 38. Shanbhag, Siddharth
    et al.
    Suliman, Salwa
    Bolstad, Anne Isine
    Stavropoulos, Andreas
    Malmö universitet, Odontologiska fakulteten (OD).
    Mustafa, Kamal
    Univ Bergen, Dept Clin Dent, Fac Med, Bergen, Norway..
    Xeno-Free Spheroids of Human Gingiva-Derived Progenitor Cells for Bone Tissue Engineering2020Inngår i: Frontiers in Bioengineering and Biotechnology, E-ISSN 2296-4185, Vol. 8, artikkel-id 968Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Gingiva has been identified as a minimally invasive source of multipotent progenitor cells (GPCs) for use in bone tissue engineering (BTE). To facilitate clinical translation, it is important to characterize GPCs in xeno-free cultures. Recent evidence indicates several advantages of three-dimensional (3D) spheroid cultures of mesenchymal stromal cells (MSCs) over conventional 2D monolayers. The present study aimed to characterize human GPCs in xeno-free 2D cultures, and to test their osteogenic potential in 3D cultures, in comparison to bone marrow MSCs (BMSCs). Primary GPCs and BMSCs were expanded in human platelet lysate (HPL) or fetal bovine serum (FBS) and characterized based onin vitroproliferation, immunophenotype and multi-lineage differentiation. Next, 3D spheroids of GPCs and BMSCs were formed via self-assembly and cultured in HPL. Expression of stemness- (SOX2, OCT4, NANOG) and osteogenesis-related markers (BMP2, RUNX2, OPN, OCN) was assessed at gene and protein levels in 3D and 2D cultures. The cytokine profile of 3D and 2D GPCs and BMSCs was assessed via a multiplex immunoassay. Monolayer GPCs in both HPL and FBS demonstrated a characteristic MSC-like immunophenotype and multi-lineage differentiation; osteogenic differentiation of GPCs was enhanced in HPL vs. FBS. CD271(+)GPCs in HPL spontaneously acquired a neuronal phenotype and strongly expressed neuronal/glial markers. 3D spheroids of GPCs and BMSCs with high cell viability were formed in HPL media. Expression of stemness- and osteogenesis-related genes was significantly upregulated in 3D vs. 2D GPCs/BMSCs; the latter was independent of osteogenic induction. Synthesis of SOX2, BMP2 and OCN was confirmed via immunostaining, andin vitromineralization via Alizarin red staining. Finally, secretion of several growth factors and chemokines was enhanced in GPC/BMSC spheroids, while that of pro-inflammatory cytokines was reduced, compared to monolayers. In summary, monolayer GPCs expanded in HPL demonstrate enhanced osteogenic differentiation potential, comparable to that of BMSCs. Xeno-free spheroid culture further enhances stemness- and osteogenesis-related gene expression, and cytokine secretion in GPCs, comparable to that of BMSCs.

    Fulltekst (pdf)
    fulltext
  • 39.
    Sinkiewicz, Gabriela
    et al.
    Malmö högskola, Fakulteten för hälsa och samhälle (HS).
    Cronholm, Sofie
    Malmö högskola, Odontologiska fakulteten (OD).
    Ljunggren, Lennart
    Malmö högskola, Fakulteten för hälsa och samhälle (HS).
    Dahlén, Gunnar
    Bratthall, Gunilla
    Malmö högskola, Odontologiska fakulteten (OD).
    Influence of dietary supplementation with Lactobacillus reuteri on the oral flora of healthy subjects2009Inngår i: Swedish Dental Journal, ISSN 0347-9994, Vol. 33, nr 4, s. 211-211, artikkel-id 18Artikkel i tidsskrift (Annet vitenskapelig)
    Abstract [en]

    Aim: The aim of this stydy was to assess whether supplementation of Lactobacillus reuteri could have an impact on the oral microbiota. Material and Methods: Twent-three healthy people aged 29 to 63 years were included. A randomised double-blind placebo-controlled study was executed consisting of 12 persons in the test group which was given the study product twice a day for twelve weeks containing L. reuteri (an equal mix of ATCC 55730 and PTA 5289 at a total of 2x108 CFU/dose) and the control group of 11 persons having corresponding placebo. Pre and post of study period plaque index and oral health status (gingivitis, probing pocket depth, bleeding on probing) were measured together with sampling of supra-, subgingival plaque and saliva collection. Microbiological analysis was done by using checkerboard DNA-DNA hybridization technique and selective culturing for lactobacilli determination. Four weeks after the last intake of the product reassessments of plaque and saliva was performed. Results: No difference in general oral health could be observed between the groups after L. reuteri supplementation. Plaque index increased significantly in the control group after twelve weeks (p= 0.023). A significant increase in total Lactobacillus counts in saliva occurred in both groups (p<0.05). The probiotic intervention resulted in a significant increase of L. reuteri (p=0.008) corresponding to 13.8% of the total lactobacilli couont. A distribution ratio of 1:4 (ATCC 55730/ATCC PTA 5289) between the two installed L. reuteri strains in saliva was noticed. Termination of intervention resulted in a wash out of L. reuteri. A significant increase was found for most bacterial species in both groups and both in supra- and subgingival plaque during the test period. There was no significant difference detected for any of the bacterial species between the groups neither in plaque location. The ratio between "bad/good" supragingival bacteria decreased for the test group, however, not significant. The corresponding ratio for subgingival bacteria decreased significantly for both groups (p= 0.05)with no significant difference between the groups. Conclusions: The supplementation of L. reuteri did not affect general oral health. Presence of L. reuteri in saliva is only temporary and washed out after termination of intervention. Microbiologically no significant effect of the probiosis was observed. It can not be concluded whether L. reuteri was established in the plaque of the test group or not.

  • 40.
    Sonnby-Borgström, Marianne
    Malmö högskola, Fakulteten för hälsa och samhälle (HS).
    Alexithymia As Related to Facial Imitation, Empathy, and Pattern of Attachment2008Konferansepaper (Fagfellevurdert)
    Abstract [en]

    The aims of this study were to investigate alexithymia in relation to facial imitation, attachment, and empathy. Pictures of facial expressions were presented to 102 participants. Electromyographic activity was measured. Self-report questionnaires were used to measure alexithymia, attachment, and empathy. High-alexithymic participants showed less imitative responses and less empathy and less secure pattern of attachment.

  • 41. Stagnér, Kerstin
    et al.
    Bratthall, Gunilla
    Malmö högskola, Odontologiska fakulteten (OD).
    Clinical evaluation of local antibiotic treatment in smokers with chronic periodontitis in a maintenance care program2009Inngår i: Swedish Dental Journal, ISSN 0347-9994, Vol. 33, nr 4, s. 201-226, artikkel-id 21Artikkel i tidsskrift (Annet vitenskapelig)
    Abstract [sv]

    Syftet med den här studien var att undersöka om lokal antibiotika gel, 8,8 % Doxycyklin (Atridox®), kan förbättra läkningen av inflammerade tandköttsfickor hos vuxna rökande patienter under stödbehandling. Material och Metod I den här experimentellt blinda randomiserade studien valdes 20 rökande patienter (≥ 10 cigaretter/dag) med diagnos kronisk parodontit ut från ett parodontalt stödbehandlingsprogram. Varje patient hade ≥ 5 tänder, och ≥ 5 tandköttsfickor med fickdjup ≥ 5 millimeter. Patienterna indelades i två grupper, 10 i testgruppen (Doxycyklingel 8,8 %, Atridox®) och 10 i kontrollgruppen (Placebogel). Vid baseline och 3 månader efter behandling utfördes följande kliniska registreringar: fickdjup (PPD), klinisk fästenivå (CAL), blödning vid sondering (BOP), och plackindex (PLI) vid 4 ytor på alla indextänder. Mätning av mängden gingivalexudat (GI) utfördes vid 2 ytor på indextänder. Tandhygienist (KS) utförde scaling och debridering i hela bettet, även molarer (full-mouth). Tandläkare behandlade omgående patienterna i testgruppen med applicering av antibiotikagel Atridox® i minst 5 tandköttsfickor med primärt fickdjup ≥ 5 millimeter och blödning vid sondering. Patienterna i kontrollgruppen fick placebogel applicerad på motsvarande sätt. Efter applikationen av gel sköljde patienterna med 0,1 % klorhexidin i 7-10 dagar då mekanisk munhygien inte fick utföras under den perioden. Statistiska analyser Statistikprogram som användes var StatXact och Excel. Statistiska metoder Wilcoxon rank och Wilcoxon tecken rank test. P-värden ≤ 0.05 har bedömts som statistiskt signifikanta. Medelvärden över alla tänder/ytor har använts för varje patient och med patienten som statistisk enhet. Resultat När studien avslutades var det 16 patienter kvar. 4 patienter exkluderades ur studien på grund av sjukdom. Dessvärre visade det sig att bortfallet endast var i testgruppen som då bestod av 4 kvinnor och 2 män med genomsnittsålder 56,3 år. I kontrollgruppen ingick 5 kvinnor och 5 män med genomsnittsålder 59,4 år. Tre månader efter behandling noterades signifikant förbättring för testgruppen avseende PPD -1.36 mm (p=0.0313), signifikant förbättring för kontrollgruppen avseende PPD -1.07 mm (p=0.0020), CAL -0.65 mm (p=0.0200) och BoP (p=0.0078). Efter 3 månader fanns det ingen signifikant skillnad mellan test- och kontrollgrupp avseende samtliga parametrar. När resultaten av samtliga tänder (inklusive indextänder) analyserades noterades signifikant förbättring av PPD för både test- (p=0.0313) och kontrollgrupp (p=0.0039). Signifikant förbättring noterades även för kontrollgruppen av BOP, CAL och GI. Ingen signifikant skillnad mellan doxycyklin- respektive placebobehandlade bett avseende samtliga parametrar. Slutsats Resultatet av den här randomiserade kontrollerade studien kunde inte visa statistisk signifikant skillnad mellan test- och kontrollgrupp i det här begränsade materialet. Lokal antibiotikabehandling med 8,8 % Doxycyklingel (Atridox®) hade inte någon tilläggseffekt till scaling och debridering hos vuxna rökare med diagnos kronisk parodontit i ett stödbehandlingsprogram. Resultatet visade god läkning och signifikant förbättring av inflammerade tandköttsfickor efter subgingival scaling och debridering i både test- och kontrollgrupp utan någon skillnad mellan antibiotika- och placebogel.

  • 42. Stagnér, Kerstin
    et al.
    Bratthall, Gunilla
    Malmö högskola, Odontologiska fakulteten (OD).
    Klinisk utvärdering av lokal antibiotikabehandling hos rökare i ett parodontalt stödbehandlingsprogram2009Inngår i: Tandhygienisttidningen, ISSN 1102-6146, Vol. 29, nr 6, s. 41-49Artikkel i tidsskrift (Annet vitenskapelig)
    Abstract [sv]

    KLINISK UTVÄRDERING AV LOKAL ANTIBIOTIKABEHANDLING HOS RÖKARE I ETT PARODONTALT STÖDBEHANDLINGSPROGRAM. Författare Kerstin Stagnér. Medförfattare Gunilla Bratthall, Avdelning för Parodontologi, Odontologiska Fakulteten, Malmö Högskola. Syfte - Syftet med den här studien var att undersöka om lokal antibiotika gel, 8,8 % Doxycyklin (Atridox®), kan förbättra läkningen av inflammerade tandköttsfickor hos vuxna rökande patienter under stödbehandling. Material och Metod. I den här experimentellt blinda randomiserade studien valdes 20 rökande patienter (≥ 10 cigaretter/dag) med diagnos kronisk parodontit ut från ett parodontalt stödbehandlingsprogram. Varje patient hade ≥ 5 tänder, och ≥ 5 tandköttsfickor med fickdjup ≥ 5 millimeter. Patienterna indelades i två grupper, 10 i testgruppen (Doxycyklingel 8,8 %, Atridox®) och 10 i kontrollgruppen (Placebogel). Vid baseline och 3 månader efter behandling utfördes följande kliniska registreringar: fickdjup (PPD), klinisk fästenivå (CAL), blödning vid sondering (BOP), och plackindex (PLI) vid 4 ytor på alla indextänder. Mätning av mängden gingivalexudat (GI) utfördes vid 2 ytor på indextänder. Tandhygienist (KS) utförde scaling och debridering i hela bettet, även molarer (full-mouth). Tandläkare behandlade omgående patienterna i testgruppen med applicering av antibiotikagel Atridox® i minst 5 tandköttsfickor med primärt fickdjup ≥ 5 millimeter och blödning vid sondering. Patienterna i kontrollgruppen fick placebogel applicerad på motsvarande sätt. Efter applikationen av gel sköljde patienterna med 0,1 % klorhexidin i 7-10 dagar då mekanisk munhygien inte fick utföras under den perioden. Statistiska analyser. Statistikprogram som användes var StatXact och Excel. Statistiska metoder Wilcoxon rank och Wilcoxon tecken rank test. P-värden ≤ 0.05 har bedömts som statistiskt signifikanta. Medelvärden över alla tänder/ytor har använts för varje patient och med patienten som statistisk enhet. Resultat. När studien avslutades var det 16 patienter kvar. 4 patienter exkluderades ur studien på grund av sjukdom. Dessvärre visade det sig att bortfallet endast var i testgruppen som då bestod av 4 kvinnor och 2 män med genomsnittsålder 56,3 år. I kontrollgruppen ingick 5 kvinnor och 5 män med genomsnittsålder 59,4 år. Tre månader efter behandling noterades signifikant förbättring för testgruppen avseende PPD -1.36 mm (p=0.0313), signifikant förbättring för kontrollgruppen avseende PPD -1.07 mm (p=0.0020), CAL -0.65 mm (p=0.0200) och BoP (p=0.0078). Efter 3 månader fanns det ingen signifikant skillnad mellan test- och kontrollgrupp avseende samtliga parametrar. När resultaten av samtliga tänder (inklusive indextänder) analyserades noterades signifikant förbättring av PPD för både test- (p=0.0313) och kontrollgrupp (p=0.0039). Signifikant förbättring noterades även för kontrollgruppen av BOP, CAL och GI. Ingen signifikant skillnad mellan doxycyklin- respektive placebobehandlade bett avseende samtliga parametrar. Slutsats. Resultatet av den här randomiserade kontrollerade studien kunde inte visa statistisk signifikant skillnad mellan test- och kontrollgrupp i det här begränsade materialet. Lokal antibiotikabehandling med 8,8 % Doxycyklingel (Atridox®) hade inte någon tilläggseffekt till scaling och debridering hos vuxna rökare med diagnos kronisk parodontit i ett stödbehandlingsprogram. Resultatet visade god läkning och signifikant förbättring av inflammerade tandköttsfickor efter subgingival scaling och debridering i både test- och kontrollgrupp utan någon skillnad mellan antibiotika- och placebogel.

    Fulltekst (pdf)
    FULLTEXT01
  • 43.
    Sternbæk, Louise
    et al.
    Malmö universitet, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV). Malmö universitet, Biofilms Research Center for Biointerfaces. Phase Holographic Imaging AB, SE-223 63 Lund, Sweden.
    Kimani, Martha
    Chemical and Optical Sensing Division, Bundesanstalt für Materialforschung und-prüfung (BAM), DE-12489 Berlin, Germany.
    Gawlitza, Kornelia
    Chemical and Optical Sensing Division, Bundesanstalt für Materialforschung und-prüfung (BAM), DE-12489 Berlin, Germany.
    Rurack, Knut
    Chemical and Optical Sensing Division, Bundesanstalt für Materialforschung und-prüfung (BAM), DE-12489 Berlin, Germany.
    Janicke, Birgit
    Phase Holographic Imaging AB, SE-223 63 Lund, Sweden.
    Alm, Kersti
    Phase Holographic Imaging AB, SE-223 63 Lund, Sweden.
    Gjörloff Wingren, Anette
    Malmö universitet, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV). Malmö universitet, Biofilms Research Center for Biointerfaces.
    Eriksson, Håkan
    Malmö universitet, Fakulteten för hälsa och samhälle (HS), Institutionen för biomedicinsk vetenskap (BMV).
    Molecularly Imprinted Polymers Exhibit Low Cytotoxic and Inflammatory Properties in Macrophages In Vitro2022Inngår i: Applied Sciences, E-ISSN 2076-3417, Vol. 12, s. 1-16, artikkel-id 6091Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Molecularly imprinted polymers (MIPs) against sialic acid (SA) have been developed as a detection tool to target cancer cells. Before proceeding to in vivo studies, a better knowledge of the overall effects of MIPs on the innate immune system is needed. The aim of this study thus was to exemplarily assess whether SA-MIPs lead to inflammatory and/or cytotoxic responses when administered to phagocytosing cells in the innate immune system. The response of monocytic/macrophage cell lines to two different reference particles, Alhydrogel and PLGA, was compared to their response to SA-MIPs. In vitro culture showed a cellular association of SA-MIPs and Alhydrogel, as analyzed by flow cytometry. The reference particle Alhydrogel induced secretion of IL-1β from the monocytic cell line THP-1, whereas almost no secretion was provoked for SA-MIPs. A reduced number of both THP-1 and RAW 264.7 cells were observed after incubation with SA-MIPs and this was not caused by cytotoxicity. Digital holographic cytometry showed that SA-MIP treatment affected cell division, with much fewer cells dividing. Thus, the reduced number of cells after SA-MIP treatment was not linked to SA-MIPs cytotoxicity. In conclusion, SA-MIPs have a low degree of inflammatory properties, are not cytotoxic, and can be applicable for future in vivo studies.

    Fulltekst (pdf)
    fulltext
  • 44. Thornton, David
    et al.
    Davies, Julia
    Malmö högskola, Odontologiska fakulteten (OD).
    Kirkham, Sara
    Gautrey, Alex
    Khan, Nagma
    Richardson, Paul
    Sheehan, John
    Identification of a nonmucin glycoprotein (gp-340) from a purified respiratory mucin preparation: evidence for an association involving the MUC5B mucin2001Inngår i: Glycobiology, ISSN 0959-6658, E-ISSN 1460-2423, Vol. 11, nr 11, s. 969-977Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Rate-zonal centrifugation of a reduced and alkylated respiratory mucin preparation identified a protein-rich fraction. This was subjected to trypsin treatment and one of the many liberated peptides was purified and its N-terminal sequence determined. The peptide was identical to a 14 amino acid sequence from the scavenger receptor cysteine-rich domain containing glycoprotein gp-340. A polyclonal antiserum, raised against the peptide, stained the serous cells in the submucosal glands of human tracheal tissue. The glycoprotein was purified from respiratory mucus by density-gradient centrifugation, gel chromatography, and anion exchange chromatography. The molecule exhibited a heterogeneous distribution of buoyant density (1.28-1.46 g/ml) that overlapped with the gel-forming mucins, was included on Sepharose CL-2B and was quite highly anionic. SDS-PAGE indicated a mass greater than 208 kDa and measurements performed across the molecular size distribution indicated an average M(r) of 5 x 10(5) with a range of M(r) from 2 x 10(5) to 1 x 10(6). Gel chromatography of respiratory mucus extracts ("associative" and "dissociative") indicated that this glycoprotein forms complexes that may involve the large gel-forming mucins MUC5AC and MUC5B. Rate zonal centrifugation suggested such complexes are more likely to involve MUC5B rather than MUC5AC mucins.

  • 45. Wallin Bengtsson, Viveca
    et al.
    Piiutainen, Eeva
    Bratthall, Gunilla
    Malmö högskola, Odontologiska fakulteten (OD).
    Alpha-1-antitrypsin deficiency and periodontitis, a pilot study2009Inngår i: Journal of clinical periodontology, Vol. 39, nr supplement 9, s. 88-88, artikkel-id 182Artikkel i tidsskrift (Annet vitenskapelig)
    Abstract [en]

    Proteases are capable of tissue breakdown. Plasma and gingival crevicular fluid (GCF) contain antiproteases, such as alfa-1-antitirypsin (AAT). Lack of AAT may lead to periodontal destruction. The aim was to study if periodontal parameters and elastase in GCF and plasma are different in AAT deficient subjects compared to subjects without AAT deficiency. Material & methods: 30 subjects were included, 20 of whom with severe AAT deficiency. Ten of them suffered from chronic obstructive pulmonary disease (group 1) and 10 were asymptomatic (group 2). Ten control subjects (group 3) were recruited from a public dental clinic. The examination comprised GCF, Gingival index (GI), Plaque Index (PlI), probing pocket depth (PPD) and radiography. GCF was collected with paper strips (Periopaper®). Plasma AAT concentration was measured by nephelometry and AAT in GCF with ELISA. Elastase activity and protein in plasma and GCF were determined by spectrophotometry. Results: The mean values for GI, PlI, PPD and the radiological measurements did not show any statistically significant differences between the groups. AAT in GCF and plasma did not show any significant difference between group 1 and 2 but a statistical difference in comparison with group 3. Elastase in GCF and plasma did not show any difference between the three groups. In conclusion no differences were found between AAT deficient subjects and healthy controls in this limited material.

    Fulltekst (pdf)
    fulltext
  • 46.
    Öztürk, Saban
    Malmö universitet, Fakulteten för hälsa och samhälle (HS).
    Hur påverkar fysisk aktivitet individens epigenom?: Litteraturstudie2022Independent thesis Basic level (degree of Bachelor), 10 poäng / 15 hpOppgave
    Abstract [sv]

    Forskningen visar att fysisk aktivitet främjar god hälsa och minskar risken för att utveckla olika typer av sjukdomar som framför allt är korrelerat till en stillasittande livsstil. I dagsläget är det svårt att i detalj beskriva hur kroppen genetiskt påverkas av olika typer av fysisk ansträngning. Det har på senare år blivit tydligare att epigenetisk modifiering har en betydande roll i hur kroppens vävnader anpassar sig till rådande yttre faktorer. Epigenetik som är ett relativt nytt område inom molekylärbiologin, beskriver hur genomet kan regleras utan att nukleotiderna förändras. Vilket innebär en förändring av genuttrycket som sker ovanpå genomet. Syftet med denna litteraturstudie är att undersöka hur fysisk aktivitet påverkar individers epigenom på specifikt skelettmuskelceller i åldersgrupperna 18–65 år, hos både män och kvinnor. Artiklarna som användes i den här litteraturstudien söktes fram med hjälp av databasen PubMed med specifikt utvalda sökord som går i linje med denna litteraturstudies syfte. Sökorden som användes var: Epigenetic AND exercise AND skeletal muscle AND Epigenetic skeletal muscle regulation. Litteraturstudien visade att en reduktion av metyleringar på ett flertal promotorregioner på DNA bildas efter ett intensivt träningspass. Detta leder i sin tur till en ökad transkription av de berörda generna. Metyleringsmönster som bildas är i hög grad korrelerad till träningsform/intensitet. Det kunde även observeras en ökning av acetyleringar på specifika gener. Endast specifika microRNA uppreglerades regelbundet efter intensiv träning som har en koppling till muskelproliferation samt differentiering. Det kunde även observeras en nedreglering av samma gener vid kroniskträning. Majoriteten av resultateten som erhölls var i direkt korrelation med träningsintensiteten. Detta är en avgörande faktor för hur bland annat metyleringsmönstret kommer att se ut samt att dessa mönster bildas snabbare än vad det tidigare har spekulerats kring.

1 - 46 of 46
RefereraExporteraLink til resultatlisten
Permanent link
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf