This study evaluated the impact of a high-intensity strength training session on rowing sprint performance. Ten male club rowers performed a baseline counter-movement jump (CMJ) and a 250 m rowing ergometer sprint (250 m). Twenty-four hours after baseline, participants performed a high-intensity strength training session (ST) consisting of multi-joint barbell exercises. At 24 h, 48 h and 72 h post-ST, CMJ and 250 m were repeated alongside assessment of plasma creatine kinase (CK) and perceived muscle soreness. At 24 h post-ST 250 m performance decreased significantly (90% CI: 0.3 – 0.7 s), but returned to pre-test values at 48 h. There was a significant decrease in CMJ height at 48 h post ST (90% CI: −0.7 – −4.1 cm). Perceived muscle soreness was significantly greater than baseline at all assessed time points following ST (p < 0.05). There was a significant increase in CK from baseline to 24 h (90% CI: 146 – 390 U/L). High-intensity strength training was observed to adversely affect 250 m rowing ergometer performance after 24 h and coincided with transient muscle damage.