A widely used method for determination of transition probabilities and oscillator strengths is based on measurements of branching fractions and radiative lifetimes. In the present work the different sources of uncertainty in branching fraction measurements using Fourier transform spectroscopy and lifetime measurements using laser induced fluorescence are discussed. A detailed description is presented of how the uncertainties should be combined to provide a well-defined uncertainty of the derived quantity. Finally, an example shows how the individual uncertainties can be presented in an “uncertainty budget”.