Purpose: The purpose of the study was to investigate certified registered nurse anesthetists’ attitude towards and utilization of evidence-based practice, and factors influencing such practice.

Design: The study had a descriptive, cross-sectional, and web-based survey design.

Methods: This study, conducted in September and October 2020, involved certified registered nurse anesthetists (n = 278) employed within 12 operation theater departments in southern Sweden. Data were collected by means of web-based questionnaires including a modified version of “Nurses’ attitudes towards research and development within nursing” (ATRAD-N, version II) with complementary questions. Descriptive statistics and manifest content analysis were used to analyze data.

Findings: A high mean value in ATRAD-N, indicating positive attitudes towards research and improvement, was reported. However, there was a low level of applying research and working with improvement activities. Factors influencing the utilization were attitude and educational level and according to the qualitative data, certified registered nurse anesthetists were limited regarding their participation due to organizational factors, such as lack of time, hierarchical structures, or lack of support from leaders.

Conclusions: Certified registered nurse anesthetists are expected to work in accordance with evidence-based practice to further develop nursing care. The main finding in the present study was that certified registered nurse anesthetists, although having a positive attitude towards research and improvement, are limited regarding their participation in evidence-based practice due to organizational factors. The findings clarify the value of continuous education for staff, and the importance of overcoming negative factors to better support certified registered nurse anesthetists in improving their ability to use evidence-based practice and increase their opportunities for integrating it into practice.