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Lipoteichoic acid extraction from plasma: Chromatography techniques utilizing truncated derivates of antimicrobial peptides
Malmö University, Faculty of Health and Society (HS), Department of Biomedical Science (BMV).
2022 (English)Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
Abstract [en]

With increasing incidence rates aligned with poor prognosis; sepsis represents one of the biggest challenges in modern health care. It is a multifactorial syndrome defined as organ dysfunction caused by disturbed systemic response to an infection. Most of the inpatient sepsis are caused by Gram positive bacteria and one of its major constituents of the cell envelope: lipoteichoic acid (LTA). An adjuvant treatment that has gained prominence recently is extracorporeal blood removal therapies i.e., hemoperfusions. The concept is to remove the bacterial virulence factors that triggers immune responses and therefor stabilize the hemodynamic parameters of the patient. The dominating research of this method centres around adsorption of the Gram negative bacterias’ endotoxin lipopolysaccharide (LPS) but not LTA, whose biochemical and physiological properties resembles each other. The aim of this study was to determine whether LTA can be adsorbed using immobilized truncated derivates of antimicrobial peptides (AMPs). LTA was quantified using ELISA comparing before and after passage through columns with immobilized peptides. Further, the absorption abilities of LTA from two different solid phases with distinctive surfaces were investigated. This was of interest to elucidate the nature of the mechanisms behind LTA extractions. All results generated inconclusive data, except for one trial which demonstrated that peptide KEF-19 adsorbed most LTA and that the electrostatic force had the greatest influence of the adsorption. Future studies should however be carried out to validate these statements as well as feasibility and safety estimations for KEF-19 as the sorbent in hemoperfusions for Gram positive bacteria and LTA.

Place, publisher, year, edition, pages
2022. , p. 25
Keywords [en]
antimicrobial peptides, chromatography, hemoperfusions, lipoteichoic acid, plasma, Sepsis
National Category
Immunology Hematology Other Chemistry Topics
Identifiers
URN: urn:nbn:se:mau:diva-52558OAI: oai:DiVA.org:mau-52558DiVA, id: diva2:1667832
Educational program
HS Biomedicinsk laboratorievetenskap
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Available from: 2022-06-13 Created: 2022-06-11 Last updated: 2022-06-13Bibliographically approved

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fulltext(689 kB)161 downloads
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Sedelius, Gustav
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CiteExportLink to record
Permanent link

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Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf