Objectives To evaluate the effect of enamel matrix derivative in liquid form (EMD-liquid) as adjunct to grafting with natural bovine bone (NBB), on new bone formation and osseointegration in buccal dehiscence defects at dental implants. Material and methods In six beagles, 3 months after extraction of the mandibular premolars and first molars. Three titanium implants (3.3 o x 8.0 mm) were inserted, and dehiscence-type defects (mesiodistal width 3 mm x 5 mm depth) were created on their buccal aspect. The defects were randomly assigned to one of the following three treatment groups: Group 1: NBB, Group 2: NBB/EMD-L, Group 3: Control. All sites were covered with a collagen membrane. Histomorphometric measurements were performed after 3 months of healing. Results New bone area, bone-to-implant contact (BIC), and first BIC (fBIC) in the NBB and NBB/EMD-L groups were significantly greater than in the control group (p < .05). Further, f-BIC was at a significantly more coronal position in the NBB + EMD-liquid group (0.4 +/- 0.1 mm) compared with the NBB group (1.2 +/- 0.2 mm). Conclusions Natural bovine bone grafting enhances bone regeneration and osseointegration at implants with buccal bone dehiscences compared with no grafting, and adjunct use of EMD-liquid appears to further enhance bone formation and osseointegration.