This study aimed to investigate the role of perceived supervisor support and organizational identification in job satisfaction among Turkish medical secretaries. The data were collected using a questionnaire form among a convenience sample of 549 secretaries working in various private hospital settings located in Istanbul. Job satisfaction was found to be positively correlated with perceived supervisor support and organizational identification partially mediated this relationship. Thus, having a supportive relationship with the supervisor increased job satisfaction, and organizational identification was found as a significant component of this relationship.