This thesis covers the animation technique rotoscoping and how abstraction of an animation’s information affects the viewer’s perception. This problem has been studied through creating three rotoscoping animations. These animations have been viewed and discussed in focus groups and the result has been analyzed through a semiotics and representation theoretical perspective. The result shows that the more abstract an animation is, the harder it is to understand meaningful information in it. This study also covers the significance of cultural and contextual aspects on how meaning is formed and shows on the result that if a viewer is familiar with the subject of the animation, the more likely it is that he or she easier could understand the animation.