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Selective detection of phospholipids using molecularly imprinted fluorescent sensory core-shell particles
Malmö University, Faculty of Health and Society (HS), Department of Biomedical Science (BMV). Department of Food Science and Engineering, School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, Nanjing, 210023, China.
Malmö University, Faculty of Health and Society (HS), Department of Biomedical Science (BMV).ORCID iD: 0000-0001-9460-0936
Malmö University, Faculty of Health and Society (HS), Department of Biomedical Science (BMV).ORCID iD: 0000-0002-8657-2928
Malmö University, Faculty of Health and Society (HS), Department of Biomedical Science (BMV).
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2020 (English)In: Scientific Reports, E-ISSN 2045-2322, Vol. 10, no 1, article id 9924Article in journal (Refereed) Published
Abstract [en]

Sphingosine-1-phosphate (S1P) is a bioactive sphingo-lipid with a broad range of activities coupled to its role in G-protein coupled receptor signalling. Monitoring of both intra and extra cellular levels of this lipid is challenging due to its low abundance and lack of robust affinity assays or sensors. We here report on fluorescent sensory core-shell molecularly imprinted polymer (MIP) particles responsive to near physiologically relevant levels of S1P and the S1P receptor modulator fingolimod phosphate (FP) in spiked human serum samples. Imprinting was achieved using the tetrabutylammonium (TBA) salt of FP or phosphatidic acid (DPPA·Na) as templates in combination with a polymerizable nitrobenzoxadiazole (NBD)-urea monomer with the dual role of capturing the phospho-anion and signalling its presence. The monomers were grafted from ca 300 nm RAFT-modified silica core particles using ethyleneglycol dimethacrylate (EGDMA) as crosslinker resulting in 10-20 nm thick shells displaying selective fluorescence response to the targeted lipids S1P and DPPA in aqueous buffered media. Potential use of the sensory particles for monitoring S1P in serum was demonstrated on spiked serum samples, proving a linear range of 18-60 µM and a detection limit of 5.6 µM, a value in the same range as the plasma concentration of the biomarker.

Place, publisher, year, edition, pages
Nature Publishing Group, 2020. Vol. 10, no 1, article id 9924
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Analytical Chemistry
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URN: urn:nbn:se:mau:diva-17599DOI: 10.1038/s41598-020-66802-3ISI: 000543973600055PubMedID: 32555511Scopus ID: 2-s2.0-85086705539OAI: oai:DiVA.org:mau-17599DiVA, id: diva2:1449023
Available from: 2020-06-29 Created: 2020-06-29 Last updated: 2024-06-17Bibliographically approved

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Li, QianjinShinde, SudhirkumarGrasso, GiulianaAbouhany, RahmaPan, GuoqingSellergren, Börje

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