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Macromolecular organization of saliva: identification of ‘insoluble’ MUC5B assemblies and non-mucin proteins in the gel phase
Malmö högskola, Faculty of Odontology (OD). Mucosal Biology Group, Department of Cell and Molecular Biology, Section for Molecular Pathogenesis, Lund University, P. O. Box 94, S-22100 Lund, Sweden.ORCID iD: 0000-0002-8183-8846
Malmö högskola, Faculty of Odontology (OD).ORCID iD: 0000-0003-3602-9449
Malmö högskola, Faculty of Odontology (OD). Mucosal Biology Group, Department of Cell and Molecular Biology, Section for Molecular Pathogenesis, Lund University, P. O. Box 94, S-22100 Lund, Sweden.ORCID iD: 0000-0001-5888-664X
Mucosal Biology Group, Department of Cell and Molecular Biology, Section for Molecular Pathogenesis, Lund University, P. O. Box 94, S-22100 Lund, Sweden.
2000 (English)In: Biochemical Journal, ISSN 0264-6021, E-ISSN 1470-8728, Vol. 351, no 2, p. 421-428Article in journal (Refereed) Published
Abstract [en]

Stimulated human submandibular’sublingual (HSMSL) and whole saliva were separated into sol and gel phases and mucins were isolated by density-gradient centrifugation in CsCl/4M guanidinium chloride. MUC5B and MUC7 were identified using anti-peptide antisera raised against sequences within the MUC5B and MUC7 apoproteins respectively. MUC7 was found mainly in the sol phase of both HSMSL and whole saliva, but some MUC7 was consistently present in the gel phase, suggesting that this mucin may interact with the salivary gel matrix. In HSMSL saliva, MUC5B was found in the gel phase ; however, most of the materialwas‘ insoluble ’inguanidiniumchlorideandwasonly brought into solution by reduction. In whole saliva, the MUC5B mucin was present both in the sol and gel phases although some materialwasagain‘ insoluble ’.Rate-zonalcentrifugationof whole saliva showed that MUC5B mucins in the sol phase were smaller than those in the gel phase, suggesting differences in oligomerization and/or degradation. Antibodies against IgA, secretory component, lysozyme and lactoferrin were used to study the distribution of non-gel-forming proteins in the different phases of saliva. The majority of these proteins was found in the sol phase of both HSMSL and whole saliva. However, a significant fraction was present in the gel phase of whole saliva, suggesting a post-secretory interaction with the salivary gel matrix. A monoclonal antibody against a parotid salivary agglutinin was used to show that this protein is present mainly in the gel phase of both whole saliva and parotid secretion.

Place, publisher, year, edition, pages
2000. Vol. 351, no 2, p. 421-428
Keywords [en]
mucin, MUC5B, saliva, gel-forming
National Category
Dentistry
Identifiers
URN: urn:nbn:se:mau:diva-15838DOI: 10.1042/0264-6021:3510421ISI: 000165080000016PubMedID: 11023828Scopus ID: 2-s2.0-0034667658Local ID: 11208OAI: oai:DiVA.org:mau-15838DiVA, id: diva2:1419360
Available from: 2020-03-30 Created: 2020-03-30 Last updated: 2024-11-26Bibliographically approved

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Wickström, ClaesChristersson, CeciliaDavies, Julia

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