Objectives: The aim of the present study was to obtain information on film forming properties by investigating adsorption of selected pellicle components and human whole saliva to different types of model surfaces, and their elutability by buffer and sodium dodecyl sulphate (SDS). Methods: Time resolved in situ ellipsometry was used to determine the adsorbed amounts of purified PRP-1, MUC5B and human whole saliva, individually and in sequence, onto pure (hydrophilic, negatively charged) and methylated (hydrophobized) silica surfaces. Results: As a general observation, the measurements show that on hydrophobic surfaces higher amounts were adsorbed than on hydrophilic ones. For the individual components, rinsing with PBS resulted in a decrease in adsorbed amounts on both model substrates. After SDS exposure a minor fraction remained adsorbed on hydrophilic surfaces, while a larger fraction was left on hydrophobic ones. Addition of HWS or MUC5B in sequence after previously adsorbed PRP-1 resulted in an increase of adsorbed amounts on both types of surfaces. Rinsing with PBS and SDS elution resulted in a decrease in the adsorbed amount of protein, although, the remaining layer may be significantly different from that of the individual, indicating interactions between adsorbed protein components. Conclusion: The different desorbability upon buffer rinsing and addition of SDS indicate that the adsorbed proteins have varying binding strengths to the two types of surfaces. The results show that salivary components from HWS as well as MUC5B adsorb after pre-adsorption of PRP-1. Interactions affecting buffer and SDS elutability may take place between sequentially adsorbed proteins.