The aim of this study was to analyse the reliability and validity of a cyberbullying behaviour questionnaire (CBQ) in working life. Further aim was to analyse the reliability and validity of a short version of a cyberbullying behaviour questionnaire (CBQ‐S) in working life. Altogether 3 371 working adults in Sweden, and 238 in the USA participated in the study. The results of the confirmatory factor analyses (CFA) showed that the one‐factor CFA model had an excellent fit to data, and Cronbach's alpha indicates that both scales are reliable. In addition, the convergent validity of the scales was demonstrated by significant correlations with the following theoretically relevant concepts: wellbeing, work engagement and intention to quit. The results of the study support the use of the CBQ and CBQ‐S as reliable and valid measures of cyberbullying behaviour in working life.