SUMMARY Fluoride release fromglass-ionomers (GI)may be important for the prevention of secondary caries. The addition of chlorhexidine gluconate (CHX) to glass-ionomer cement (3%) adds an effect that enables it to be used as a varnish for the temporary coating of surfaces at risk for caries. This study investigated the fluoride release pattern and antibacterial effect of such a material. Glassionomer luting cement powder (Aqua-Cem) was mixed with water, 10% CHX or 10% CHX with 11% tartaric acid (TA), respectively, to test specimens (6 * 1.5 mm). After setting, the specimens were immersed in 10 ml deionized water and transferred to new vials after various intervals over a period of two months. The antibacterial effect towards mutans streptococci was assessed using agar diffusion. The fluoride release was measured after two hours and after shifting the specimens to new vials 10 times during the two-month period. The total fluoride release was 69.02, 50.64 and 48.56 μg/cm2 from each specimen in the GI, GI-CHX and the GI-CHX-TA groups, respectively. For two-hour old specimens, the mean inhibition zone was 0, 50, 36 mm2 in the GI, GI-CHX and GICHX- TA groups, respectively, and, after two months, 45 mm2 in the GI-CHX group and 19 mm2 in the GI-CHX-TA group. It can be concluded that the addition of CHX and CHX-TA adds antibacterial properties to GI and the release of fluoride is decreased.