Malmö University Publications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Lipid Bilayer-like Mixed Self-Assembled Monolayers with Strong Mobility and Clustering-Dependent Lectin Affinity
Malmö University, Faculty of Health and Society (HS), Department of Biomedical Science (BMV). Malmö University, Biofilms Research Center for Biointerfaces.
Malmö University, Faculty of Health and Society (HS), Department of Biomedical Science (BMV). Malmö University, Biofilms Research Center for Biointerfaces.
Division of Physical Chemistry , Lund University , Box 124, 22100 Lund , Sweden.
Division of Physical Chemistry , Lund University , Box 124, 22100 Lund , Sweden.
Show others and affiliations
2019 (English)In: Langmuir, ISSN 0743-7463, E-ISSN 1520-5827, Vol. 35, no 24, p. 8174-8181Article in journal (Refereed) Published
Abstract [en]

Glycans at the surface of cellular membranes modulate biological activity via multivalent association with extracellular messengers. The lack of tuneable simplified models mimicking this dynamic environment complicates basic studies of these phenomena. We here present a series of mixed reversible self-assembled monolayers (rSAMs) that addresses this deficiency. Mixed rSAMs were prepared in water by simple immersion of a negatively charged surface in a mixture of sialic acid- and hydroxy-terminated benzamidine amphiphiles. Surface compositions derived from infrared reflection-absorption spectroscopy (IRAS) and film thickness information (atomic force microscopy, ellipsometry) suggest the latter to be statistically incorporated in the monolayer. These surfaces' affinity for the lectin hemagglutinin revealed a strong dependence of the affinity on the presentation, density, and mobility of the sialic acid ligands. Hence, a spacer length of 4 ethylene glycol and a surface density of 15% resulted in a dissociation constant K-d,K-multi of 1.3 x 10(-13) M, on par with the best di- or tri-saccharide-based binders reported to date, whereas a density of 20% demonstrated complete resistance to hemagglutinin binding. These results correlated with ligand mobility measured by fluorescence recovery after photobleaching which showed a dramatic drop in the same interval. The results have a direct bearing on biological cell surface multivalent recognition involving lipid bilayers and may guide the design of model surfaces and sensors for both fundamental and applied studies.

Place, publisher, year, edition, pages
American Chemical Society (ACS), 2019. Vol. 35, no 24, p. 8174-8181
Keywords [en]
Chemistry, Multidisciplinary, Chemistry, Physical, Materials Science, Multidisciplinary
National Category
Natural Sciences
Identifiers
URN: urn:nbn:se:mau:diva-5496DOI: 10.1021/acs.langmuir.9b01452ISI: 000472682600063PubMedID: 31117738Scopus ID: 2-s2.0-85067030050Local ID: 30218OAI: oai:DiVA.org:mau-5496DiVA, id: diva2:1402356
Available from: 2020-02-28 Created: 2020-02-28 Last updated: 2023-08-15Bibliographically approved
In thesis
1. Stimuli-responsive lipid bilayer mimics for protein, virus and cell recognition
Open this publication in new window or tab >>Stimuli-responsive lipid bilayer mimics for protein, virus and cell recognition
2018 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

The most well-studied two-dimensional biomimetic cellular membrane modelsare self-assembled monolayers (SAMs) and supported lipid bilayers (SLBs).The former has the advantage of control over ligand density, homogeneity andorientation, allowing unambiguous interaction studies. It however lacks longrangelateral mobility, which is one of the most important aspects of cellularmembranes. SLBs are laterally mobile but they are fragile and instable uponexposure to air. Literature examples that contain all the above desirablecharacteristics with stimuli-responsiveness to fabricate biomaterials forbiosensing or modulating cell adhesion are rare. We here report on anadaptable platform, reversible self-assembled monolayers (rSAMs), featuringstrongly enhanced affinity towards influenza viruses as compared to SAMs,lateral mobility to investigate glycan-lectin interactions and tunable surfacedynamics to modulate cell adhesion. This new system utilizes noncovalentamidinium-carboxylate ion pairs for building up stable and ordered twodimensionalassemblies, akin to lipid bilayers but with a simple preparationprocess, stimuli-responsiveness and fast on/off rates.

Place, publisher, year, edition, pages
Malmö university, Faculty of Health and Society, 2018. p. 68
Series
Malmö University Health and Society Dissertations, ISSN 1653-5383 ; 7
Keywords
Lipid bilayers, Adaptable, Self-assembled, Monolayers, Supramolecular chemistry, Cell adhesion, Biocensor
National Category
Natural Sciences
Identifiers
urn:nbn:se:mau:diva-7321 (URN)10.24834/2043/24967 (DOI)24967 (Local ID)9789171049261 (ISBN)9789171049278 (ISBN)24967 (Archive number)24967 (OAI)
Available from: 2020-02-28 Created: 2020-02-28 Last updated: 2023-07-06Bibliographically approved

Open Access in DiVA

fulltext(1742 kB)151 downloads
File information
File name FULLTEXT01.pdfFile size 1742 kBChecksum SHA-512
d96d099f3d4e31cd9b7db01d2d519a9bb52381d2957d90cbd74bef311ea128a0df0c203ae62ef5019a64f9cc98e5c2481ee99d6bfbe3b08a554db56a50bd1b1f
Type fulltextMimetype application/pdf

Other links

Publisher's full textPubMedScopus

Authority records

Yeung, Sing YeePan, GuoqingSellergren, Börje

Search in DiVA

By author/editor
Yeung, Sing YeeSergeeva, YuliaPan, GuoqingChaturvedi, VivekSellergren, Börje
By organisation
Department of Biomedical Science (BMV)Biofilms Research Center for Biointerfaces
In the same journal
Langmuir
Natural Sciences

Search outside of DiVA

GoogleGoogle Scholar
Total: 151 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 108 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf