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Ultra-stable zeolites - a tool for in-cell chemistry
Malmö högskola, School of Technology (TS).
Malmö högskola, School of Technology (TS).ORCID iD: 0000-0003-0769-9988
2004 (English)In: Journal of Biotechnology, ISSN 0168-1656, E-ISSN 1873-4863, Vol. 111, no 3, p. 279-290Article in journal (Refereed) Published
Abstract [en]

Ultrastable zeolite particles were used as vehicles to carry low molecular bio-active substances and macromolecules as proteins into viable cells. Zeolite particles that can be used for internalisation by phagocytosis were obtained from the non-sedimenting fraction of a commercially available zeolite preparation after 1 × g sedimentation. Protein adsorbed on the zeolite surface was shown to enter the endosomal pathway after phagocytosis and could be cleaved by the endosomal proteases. As a model of a low molecular weight bio-active molecule, the inhibitor of the cellular synthesis of nitrogen oxide, N-nitro-l-arginine methyl ester (L-NAME), was used. A partial inhibition of the cellular NO production was shown after utilizing zeolites as vehicles to introduce the inhibitor into the cells. A targeting of the intra-cellular enzymes that was at least 10 times more efficient was obtained by the use of zeolites as a carrier of the inhibitor, as opposed to addition of the inhibitor to the culture medium.

Place, publisher, year, edition, pages
Elsevier, 2004. Vol. 111, no 3, p. 279-290
National Category
Chemical Engineering
Identifiers
URN: urn:nbn:se:mau:diva-4195DOI: 10.1016/j.jbiotec.2004.04.008ISI: 000222954500006PubMedID: 15246664Scopus ID: 2-s2.0-3042799378Local ID: 2733OAI: oai:DiVA.org:mau-4195DiVA, id: diva2:1401025
Available from: 2020-02-28 Created: 2020-02-28 Last updated: 2024-08-20Bibliographically approved

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Eriksson, Håkan

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