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Determination of Sorption Isotherm and Rheological Properties of Lysozyme Using a High-Resolution Humidity Scanning QCM-D Technique
Malmö högskola, Faculty of Health and Society (HS), Department of Biomedical Science (BMV).ORCID iD: 0000-0002-9852-5440
2013 (English)In: Journal of Physical Chemistry B, ISSN 1520-6106, E-ISSN 1520-5207, Vol. 117, no 34, p. 10017-10026Article in journal (Refereed)
Abstract [en]

The high-resolution humidity scanning QCM-D technique enables investigation of hydration of soft matter films using a quartz crystal microbalance with dissipation monitoring (QCM-D) equipped with a humidity module. Based on a continuous increase of relative humidity, properties of soft matter films can be investigated depending on the water content of the surrounding atmosphere. Determination of complete water sorption isotherms is possible via analysis of the overtone dependence of the resonance frequencies. Rheological properties are monitored via measurement of the dissipation. The glass transition can be identified from the change of viscoelastic properties of the film reflected in changes of the dissipation. A high-resolution water sorption isotherm of lysozyme was measured and compared with results from water sorption calorimetry. Analysis of the rheological behavior during hydration of lysozyme films revealed the presence of two separate sharp transitions at the water activities 0.67 and 0.91, which are connected to the glass transition. In previous works, only the existence of a broad glass transition has been reported so far. Combining the QCM-D data with Raman scattering data presented earlier, a new mechanism of isothermal glass transition in lysozyme is proposed.

Place, publisher, year, edition, pages
American Chemical Society (ACS), 2013. Vol. 117, no 34, p. 10017-10026
Keywords [en]
Hydration, Lysozyme, Sorption Calorimetry, QCM-D, quartz crystal microbalance with dissipation monitoring, Humidity, water content, LiCl, Glass transition, Rheology, G'/G''
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Natural Sciences
Identifiers
URN: urn:nbn:se:mau:diva-4168DOI: 10.1021/jp404138fISI: 000323917100020Local ID: 16490OAI: oai:DiVA.org:mau-4168DiVA, id: diva2:1400998
Available from: 2020-02-28 Created: 2020-02-28 Last updated: 2022-06-27Bibliographically approved

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Kocherbitov, Vitaly

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