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Antibody–antigen interaction on polystyrene: An in situ ellipsometric study
Malmö högskola, Faculty of Health and Society (HS).
Malmö högskola, Faculty of Health and Society (HS).
2012 (English)In: Journal of Colloid and Interface Science, ISSN 0021-9797, E-ISSN 1095-7103, Vol. 368, no 1, p. 533-539Article in journal (Refereed) Published
Abstract [en]

The objective of this investigation was to monitor the adsorption of antibodies to polystyrene surfaces using ellipsometry. Commercial polystyrene slides used for solid state diagnostics were selected as substrates and the adsorption of three different antibodies (human IgG, bovine IgG and goat anti-human IgG) were evaluated. Based on theoretical models describing the ellipsometric data, it was concluded that the adsorption of antibodies should result in layers that are sufficiently thick to be able to monitor the adsorption in terms of adsorbed amount and thickness of the layer with a reasonable precision. The experimental results confirmed this assumption and values of 2.0–2.3 mg/m2 were detected for the adsorbed amount with a corresponding thickness of 10–16 nm. It was furthermore found that the antibodies bound irreversibly with respect to rinsing with protein-free solutions. In additional experiments, the consecutive incubation of human IgG and anti-human IgG was investigated. These results showed that, on average, approximately half of the surface immobilized anti-human IgG molecules are capable of binding to human IgG during its incubation. From the consecutive binding experiments it could also be concluded that antibodies present in the polyclonal anti-human IgG preparation were capable of binding to around four different epitopes on the human IgG. A final set of experiments addressed the stability of adsorbed human IgG layers with respect to drying and incubation with surfactant. The results revealed that the adsorbed antibody layer is relatively resistant to these treatments.

Place, publisher, year, edition, pages
Elsevier, 2012. Vol. 368, no 1, p. 533-539
Keywords [en]
Adsorption, Antibody, Polystyrene, Ellipsometry, Immunoassays, ELISA
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:mau:diva-3955DOI: 10.1016/j.jcis.2011.11.034ISI: 000299490500069PubMedID: 22172693Scopus ID: 2-s2.0-84855849728Local ID: 13069OAI: oai:DiVA.org:mau-3955DiVA, id: diva2:1400779
Available from: 2020-02-28 Created: 2020-02-28 Last updated: 2024-02-05Bibliographically approved

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Svensson, OlofArnebrant, Thomas

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