Malmö University Publications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Increased levels of glycosamanoglycans during septic shock: relation to mortality and the antibacterial actions of plasma
Malmö högskola, Faculty of Health and Society (HS).
Show others and affiliations
2008 (English)In: Shock, ISSN 1073-2322, E-ISSN 1540-0514, Vol. 30, no 6, p. 623-627Article in journal (Refereed)
Abstract [en]

Glycosaminoglycans (GAGs) are structurally heterogeneous negatively charged polysaccharides. Endothelial GAGs, also known as glycocalyx, are involved in capillary permeability. In rat venules stimulated with proinflammatory substances ex vivo, the GAG-containing proteoglycan, syndecan-1, is shed from the endothelium. We wanted to investigate if we could trace the same response during septic shock as reflected in the circulating GAG levels. Arterial plasma samples were collected from 18 consecutive septic shock patients admitted to our intensive care unit. Plasma GAGs were measured with an Alcian blue slot binding assay, and syndecan-1 levels were measured with enzymelinked immunosorbent assay. Effects of GAGs on the antibacterial activity of plasma were assessed by a radial diffusion assay. The median plasma GAG level was significantly higher in the septic shock patients than in matched controls (median [interquartile range], 2.7 2g/mL [1.9 Y 4.8 2g/mL] vs. 1.8 2g/mL [1.7 Y 2.0 2g/mL]). Furthermore, the GAG levels were significantly higher in nonsurvivors (4.6 2g/mL [3.1 Y 8.8 2g/mL], n = 8) than survivors (1.8 2g/mL [1.6 Y 2.6 2g/mL], n = 10). The syndecan-1 levels were also increased in the patients compared with controls (246 ng/mL [180 Y 496 ng/mL] vs. 26 ng/mL [23 Y 31 ng/mL]) and correlated to the cardiovascular Sequential Organ Failure Assessment (SOFA) score. The GAGs inhibited the endogenous antibacterial activity of plasma as well as isolated antimicrobial peptides. The concentrations required were in the same range as the GAG levels measured in the patients. These results show that the GAG levels are increased in septic shock patients, possibly reflecting peripheral endothelial cell damage. We also found that GAGs in relevant concentrations neutralize antimicrobial peptides in plasma.

Place, publisher, year, edition, pages
Biomedical press , 2008. Vol. 30, no 6, p. 623-627
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:mau:diva-3941DOI: 10.1097/SHK.0b013e3181777da3ISI: 000261032600003PubMedID: 18497712Scopus ID: 2-s2.0-54849433850Local ID: 7331OAI: oai:DiVA.org:mau-3941DiVA, id: diva2:1400764
Available from: 2020-02-28 Created: 2020-02-28 Last updated: 2024-02-05Bibliographically approved

Open Access in DiVA

No full text in DiVA

Other links

Publisher's full textPubMedScopus

Authority records

Ljunggren, Lennart

Search in DiVA

By author/editor
Ljunggren, Lennart
By organisation
Faculty of Health and Society (HS)
In the same journal
Shock
Medical and Health Sciences

Search outside of DiVA

GoogleGoogle Scholar

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 23 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf