Multi-modal image registration and machine learning for the generation of 3D virtual histology of bone implants Show others and affiliations
2024 (English) In: Developments in X-Ray Tomography XV, SPIE - The International Society for Optics and Photonics, 2024Conference paper, Published paper (Refereed)
Abstract [en]
In our correlative characterisation studies of biodegradable and permanent metal bone implants, we have performed both synchrotron-radiation microtomography (SR-µCT) and histology on the same samples. Histological staining is still the gold standard for tissue visualisation yet requires multiple time-consuming sample preparation steps (fixing, embedding, sectioning and staining) before imaging is performed on individual slices, in contrast to the non-invasive and 3D nature of tomography. In the process of correlating the corresponding data sets, we are able to combine advantages of both modalities by using machine learning methods to generate artificially stained 3D virtual histology datasets from SR-µCT datasets. For this we have developed an automated registration tool to find and fit the correct virtual tomographic plane to each histology slice. Preliminary results are promising after training a modified cycle generative adversarial network on our data, with two different histological stainings.
Place, publisher, year, edition, pages SPIE - The International Society for Optics and Photonics, 2024.
Series
Proceedings of SPIE, the International Society for Optical Engineering, ISSN 0277-786X ; 13152
Keywords [en]
artificially stained virtual histology, automated image registration, biodegradable metal bone implants, correlative characterisation, correlative imaging, cycleGAN, machine learning, multi-modal imaging, Biological implants, Computerized tomography, Image segmentation, Medical imaging, Metal implants, Noninvasive medical procedures, Synchrotron radiation, Biodegradable metal bone implant, Biodegradable metals, Bone implant, Correlative characterization, Images registration, Machine-learning, Multimodal imaging, Virtual histologies, Bone
National Category
Medical Imaging
Identifiers URN: urn:nbn:se:mau:diva-72968 DOI: 10.1117/12.3028465 Scopus ID: 2-s2.0-85212499617 ISBN: 9781510679641 (electronic) OAI: oai:DiVA.org:mau-72968 DiVA, id: diva2:1925147
Conference 15th SPIE Conference on Developments in X-Ray Tomography; 19-22 August 2024, San Diego, California, United States
2025-01-082025-01-082025-02-09 Bibliographically approved