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Formation and Analysis of Mono-species and Polymicrobial Oral Biofilms in Flow-Cell Models
Malmö University, Faculty of Odontology (OD).
Malmö University, Faculty of Odontology (OD).ORCID iD: 0000-0003-3173-7577
Malmö University, Faculty of Odontology (OD).
Malmö University, Faculty of Odontology (OD).ORCID iD: 0000-0001-9545-9161
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2023 (English)In: Bacterial Pathogenesis: Methods and Protocols,, Springer, 2023, p. 33-52Chapter in book (Refereed)
Abstract [en]

The oral microbiota, which is known to include at least 600 different bacterial species, is found on the teethand mucosal surfaces as multi-species communities or biofilms. The oral surfaces are covered with a pellicleof proteins absorbed from saliva, and biofilm formation is initiated when primary colonizers, which expresssurface adhesins that bind to specific salivary components, attach to the oral tissues. Further developmentthen proceeds through co-aggregation of additional species. Over time, the composition of oral biofilms,which varies between different sites throughout the oral cavity, is determined by a combination ofenvironmental factors such as the properties of the underlying surface, nutrient availability and oxygenlevels, and bacterial interactions within the community. A complex equilibrium between biofilm communities and the host is responsible for the maintenance of a healthy biofilm phenotype (eubiosis). In the faceof sustained environmental perturbation, however, biofilm homeostasis can break down giving rise todysbiosis, which is associated with the development of oral diseases such as caries and periodontitis.In vitro models have an important part to play in increasing our understanding of the complex processesinvolved in biofilm development in oral health and disease, and the requirements for experimental system,microbial complexity, and analysis techniques will necessarily vary depending on the question posed. In thischapter we describe some current and well-established methods used in our laboratory for studying oralbacteria in biofilm models which can be adapted to suit the needs of individual users. 

Place, publisher, year, edition, pages
Springer, 2023. p. 33-52
Series
Methods in Molecular Biology, E-ISSN 1940-6029 ; 2674
National Category
Cell and Molecular Biology
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URN: urn:nbn:se:mau:diva-62874DOI: 10.1007/978-1-0716-3243-7_2PubMedID: 37258958Scopus ID: 2-s2.0-85160680476ISBN: 978-1-0716-3242-0 (print)ISBN: 978-1-0716-3243-7 (electronic)OAI: oai:DiVA.org:mau-62874DiVA, id: diva2:1801076
Available from: 2023-09-29 Created: 2023-09-29 Last updated: 2023-10-06Bibliographically approved

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Neilands, JessicaSvensäter, GunnelRobertsson, CarolinaWickström, ClaesDavies, Julia R

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Neilands, JessicaSvensäter, GunnelBoisen, GabriellaRobertsson, CarolinaWickström, ClaesDavies, Julia R
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