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A Rapidly Responsive Sensor for Wireless Detection of Early and Mature Microbial Biofilms.
Malmö University, Faculty of Health and Society (HS), Department of Biomedical Science (BMV). Malmö University, Biofilms Research Center for Biointerfaces.
Polytechnic University of Cartagena: Universidad Politecnica de Cartagena, Department of Applied Physics, SPAIN.
HES-SO Valais Wallis, Institute of system engineering, SWITZERLAND.
University of Bologna Department of Industrial Chemistry Toso Montanari: Universita degli Studi di Bologna Dipartimento di Chimica Industriale Toso Montanari, Department of Industrial Chemistry, ITALY.
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2023 (English)In: Angewandte Chemie International Edition, ISSN 1433-7851, E-ISSN 1521-3773, Vol. 62, no 40, article id e202308181Article in journal (Refereed) Published
Abstract [en]

Biofilm-associated infections, which are able to resist antibiotics, pose a significant challenge in clinical treatments. Such infections have been linked to various medical conditions, including chronic wounds and implant-associated infections, making them a major public-health concern. Early-detection of biofilm formation offers significant advantages in mitigating adverse effects caused by biofilms. In this work, we aim to explore the feasibility of employing a novel wireless sensor for tracking both early-stage and matured-biofilms formed by the medically relevant bacteria Staphylococcus aureus and Pseudomonas aeruginosa. The sensor utilizes electrochemical reduction of an AgCl layer bridging two silver legs made by inkjet-printing, forming a part of near-field-communication tag antenna. The antenna is interfaced with a carbon cloth designed to promote the growth of microorganisms, thereby serving as an electron source for reduction of the resistive AgCl into a highly-conductive Ag bridge. The AgCl-Ag transformation significantly alters the impedance of the antenna, facilitating wireless identification of an endpoint caused by microbial growth. To the best of our knowledge, this study for the first time presents the evidence showcasing that electrons released through the actions of bacteria can be harnessed to convert AgCl to Ag, thus enabling the wireless, battery-less, and chip-less early-detection of biofilm formation.

Place, publisher, year, edition, pages
John Wiley & Sons, 2023. Vol. 62, no 40, article id e202308181
Keywords [en]
Microbial biofilm, chip-less wireless sensing, inkjet printing, mediated electron transfer, near field communication
National Category
Microbiology
Identifiers
URN: urn:nbn:se:mau:diva-62039DOI: 10.1002/anie.202308181ISI: 001090146000021PubMedID: 37490019Scopus ID: 2-s2.0-85168699269OAI: oai:DiVA.org:mau-62039DiVA, id: diva2:1790267
Available from: 2023-08-22 Created: 2023-08-22 Last updated: 2024-04-17Bibliographically approved
In thesis
1. Development of Wireless Biosensors Integrated into the Radio Frequency Antenna for Chipless and Battery-less Monitoring of Biological Reactions
Open this publication in new window or tab >>Development of Wireless Biosensors Integrated into the Radio Frequency Antenna for Chipless and Battery-less Monitoring of Biological Reactions
2023 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Development of wireless sensors and biosensors is currently experiencing a rapid progress with a substantial focus directed toward highlighting their potential applications as non-invasive wearables, implants, and highly mobile point-of-care devices. Integration of wireless biosensors into the Internet of Things (IoT) is widely acknowledged as a technological advancement with the potential to significantly change daily life. To maximize this potential, simple integration of biosensors with wireless communication elements would be advantageous. In this regard, systems functioning in chipless, and battery-less modes outperform integrated circuit (IC) based and battery-powered wireless biosensors. Nevertheless, the accessibility of these wireless designs is still limited. In this study, we present a novel approach where incorporating silver nanoparticles(AgNPs) as a part of the radio frequency (RF) tag antenna enables the realization of simple, chipless, and battery-less wireless sensing of biological oxidation and reduction reactions. We exemplified the mechanism of operation in such systems by electronic wiring of enzymes through direct electron transfer (DET) and microorganisms through mediated electron transfer (MET) to the redox conversion of Ag/AgCl. The wiring was designed to facilitate the transformation of metallic AgNPs into AgCl (Ag → AgCl) or the conversion of AgCl particles back into metallic AgNPs (AgCl → Ag) when the enzymatic/microorganism based electron transfer reactions were present. These reactions occurring on the biosensor RF tag antenna strongly changed the impedance of the tag, which was wirelessly monitored by a radio frequency identification (RFID) reader. The functionality of the proposed setup in direct electron transfer coupling of the enzymatic reactions to the redox conversion of the Ag/AgCl was demonstrated by wireless detection of glucose in whole blood samples and hydrogen peroxide penetrated through the skin membrane using the enzymes glucose dehydrogenase(GDH) and horseradish peroxidase (HRP). Additionally, the capability of the proposed configuration in mediated electron transfer wiring of microorganisms to the Ag/AgCl electrochemistry was shown by wireless monitoring of medically relevant microbial biofilms in simulated wound fluid. Generalizing, the results of this work, for the first time, demonstrated that exploiting Ag/AgCl as a part of the tag antenna allows simple, chipless, and battery-less wireless sensing of biological oxidation and reduction reactions.

Place, publisher, year, edition, pages
Malmö: Malmö University Press, 2023. p. 108
Series
Malmö University Health and Society Dissertations, ISSN 1653-5383 ; 99
National Category
Chemical Sciences Engineering and Technology Medical Biotechnology
Identifiers
urn:nbn:se:mau:diva-63289 (URN)10.24834/isbn.9789178774128 (DOI)9789178774111 (ISBN)9789178774128 (ISBN)
Public defence
2023-10-27, AS: E002, Faculty of Health and Society, 09:15
Opponent
Supervisors
Note

Paper IV in dissertation as manuscript.

Available from: 2023-10-31 Created: 2023-10-30 Last updated: 2024-02-27Bibliographically approved

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Shafaat, AtefehLopes da Silva, ZitaNeilands, JessicaSotres, JavierBjörklund, SebastianRuzgas, Tautgirdas

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Shafaat, AtefehLopes da Silva, ZitaNeilands, JessicaSotres, JavierBjörklund, SebastianRuzgas, Tautgirdas
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