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Limosilactobacillus reuteri inhibits the acid tolerance response in oral bacteria
Malmö University, Faculty of Odontology (OD). Malmö University, Biofilms Research Center for Biointerfaces.
Malmö University, Faculty of Odontology (OD). Malmö University, Faculty of Health and Society (HS), Department of Biomedical Science (BMV).
Malmö University, Faculty of Health and Society (HS), Department of Biomedical Science (BMV). Malmö University, Biofilms Research Center for Biointerfaces.
Malmö University, Faculty of Odontology (OD).
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2023 (English)In: Biofilm, E-ISSN 2590-2075, Vol. 6, article id 100136Article in journal (Refereed) Published
Abstract [en]

Probiotic bacteria show promising results in prevention of the biofilm-mediated disease caries, but the mechanisms are not fully understood. The acid tolerance response (ATR) allows biofilm bacteria to survive and metabolize at low pH resulting from microbial carbohydrate fermentation. We have studied the effect of probiotic strains: Limosilactobacillus reuteri and Lacticaseibacillus rhamnosus on ATR induction in common oral bacteria. Communities of L. reuteri ATCC PTA5289 and Streptoccus gordonii, Streptococcus oralis, Streptococcus mutans or Actinomyces naeslundii in the initial stages of biofilm formation were exposed to pH 5.5 to allow ATR induction, followed by a low pH challenge. Acid tolerance was evaluated as viable cells after staining with LIVE/ DEAD & REG;BacLightTM. The presence of L. reuteri ATCC PTA5289 caused a significant reduction in acid tolerance in all strains except S. oralis. When S. mutans was used as a model organism to study the effects of additional probiotic strains (L. reuteri SD2112, L. reuteri DSM17938 or L. rhamnosus GG) as well as L. reuteri ATCC PTA5289 supernatant on ATR development, neither the other probiotic strains nor supernatants showed any effect. The presence of L. reuteri ATCC PTA5289 during ATR induction led to down-regulation of three key genes involved in tolerance of acid stress (luxS, brpA and ldh) in Streptococci. These data suggest that live cells of probiotic L. reuteri ATCC PTA5289 can interfere with ATR development in common oral bacteria and specific strains of L. reuteri may thus have a role in caries prevention by inhibiting development of an acid-tolerant biofilm microbiota.

Place, publisher, year, edition, pages
Elsevier, 2023. Vol. 6, article id 100136
Keywords [en]
Probiotics, Acid tolerance, Caries, Early oral biofilms, Pioneer species
National Category
Dentistry
Identifiers
URN: urn:nbn:se:mau:diva-61921DOI: 10.1016/j.bioflm.2023.100136ISI: 001038416000001PubMedID: 37408693Scopus ID: 2-s2.0-85163191080OAI: oai:DiVA.org:mau-61921DiVA, id: diva2:1788587
Available from: 2023-08-16 Created: 2023-08-16 Last updated: 2023-12-07Bibliographically approved

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Boisen, GabriellaPrgomet, ZdenkaEnggren, GabrielaDavies, Julia R

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Boisen, GabriellaPrgomet, ZdenkaEnggren, GabrielaDavies, Julia R
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Faculty of Odontology (OD)Biofilms Research Center for BiointerfacesDepartment of Biomedical Science (BMV)
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