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Metabolic Reprogramming of Macrophages upon In Vitro Incubation with Aluminum-Based Adjuvant
Malmö University, Faculty of Health and Society (HS), Department of Biomedical Science (BMV).
Malmö University, Faculty of Health and Society (HS), Department of Biomedical Science (BMV). Univ Clermont Auvergne, Polytech Clermont Ferrand, F-63001 Clermont ferrand, France..
Malmö University, Faculty of Health and Society (HS), Department of Biomedical Science (BMV).
Malmö University, Faculty of Health and Society (HS), Department of Biomedical Science (BMV).ORCID iD: 0000-0003-0769-9988
2023 (English)In: International Journal of Molecular Sciences, ISSN 1661-6596, E-ISSN 1422-0067, Vol. 24, no 5, article id 4409Article in journal (Refereed) Published
Abstract [en]

Aluminum-based adjuvants have been extensively used in vaccines. Despite their widespread use, the mechanism behind the immune stimulation properties of these adjuvants is not fully understood. Needless to say, extending the knowledge of the immune-stimulating properties of aluminum-based adjuvants is of utmost importance in the development of new, safer, and efficient vaccines. To further our knowledge of the mode of action of aluminum-based adjuvants, the prospect of metabolic reprogramming of macrophages upon phagocytosis of aluminum-based adjuvants was investigated. Macrophages were differentiated and polarized in vitro from human peripheral monocytes and incubated with the aluminum-based adjuvant Alhydrogel((R)). Polarization was verified by the expression of CD markers and cytokine production. In order to recognize adjuvant-derived reprogramming, macrophages were incubated with Alhydrogel((R)) or particles of polystyrene as control, and the cellular lactate content was analyzed using a bioluminescent assay. Quiescent M0 macrophages, as well as alternatively activated M2 macrophages, exhibited increased glycolytic metabolism upon exposure to aluminum-based adjuvants, indicating a metabolic reprogramming of the cells. Phagocytosis of aluminous adjuvants could result in an intracellular depot of aluminum ions, which may induce or support a metabolic reprogramming of the macrophages. The resulting increase in inflammatory macrophages could thus prove to be an important factor in the immune-stimulating properties of aluminum-based adjuvants.

Place, publisher, year, edition, pages
MDPI, 2023. Vol. 24, no 5, article id 4409
Keywords [en]
aluminum adjuvant, macrophages, metabolic reprogramming, lactate
National Category
Medical Biotechnology
Identifiers
URN: urn:nbn:se:mau:diva-59243DOI: 10.3390/ijms24054409ISI: 000947001700001PubMedID: 36901849Scopus ID: 2-s2.0-85149854014OAI: oai:DiVA.org:mau-59243DiVA, id: diva2:1751162
Available from: 2023-04-17 Created: 2023-04-17 Last updated: 2024-02-05Bibliographically approved

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Danielsson, RaviMile, IreneEriksson, Håkan

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