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Expanding the Toolbox for Bicelle-Forming Surfactant–Lipid Mixtures
Malmö University, Biofilms Research Center for Biointerfaces. Malmö University, Faculty of Health and Society (HS), Department of Biomedical Science (BMV). Plant Biochemistry, Department of Plant and Environmental Sciences, University of Copenhagen, Thorvaldsensvej 40, Copenhagen, DK-1871, Denmark.ORCID iD: 0000-0002-9927-9413
Malmö University, Faculty of Health and Society (HS), Department of Biomedical Science (BMV). Malmö University, Biofilms Research Center for Biointerfaces.ORCID iD: 0000-0003-3178-4867
Plant Biochemistry, Department of Plant and Environmental Sciences, University of Copenhagen, Thorvaldsensvej 40, DK-1871 Copenhagen, Denmark.ORCID iD: 0000-0002-6493-2259
European Molecular Biology Laboratory (EMBL) Hamburg Outstation, DESY, 22607 Hamburg, Germany.
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2022 (English)In: Molecules, ISSN 1431-5157, E-ISSN 1420-3049, Vol. 27, no 21, p. 7628-7628Article in journal (Refereed) Published
Abstract [en]

Bicelles are disk-shaped models of cellular membranes used to study lipid–protein interactions, as well as for structural and functional studies on transmembrane proteins. One challenge for the incorporation of transmembrane proteins in bicelles is the limited range of detergent and lipid combinations available for the successful reconstitution of proteins in model membranes. This is important, as the function and stability of transmembrane proteins are very closely linked to the detergents used for their purification and to the lipids that the proteins are embedded in. Here, we expand the toolkit of lipid and detergent combinations that allow the formation of stable bicelles. We use a combination of dynamic light scattering, small-angle X-ray scattering and cryogenic electron microscopy to perform a systematic sample characterization, thus providing a set of conditions under which bicelles can be successfully formed.

Place, publisher, year, edition, pages
MDPI, 2022. Vol. 27, no 21, p. 7628-7628
National Category
Biochemistry and Molecular Biology
Research subject
Health and society
Identifiers
URN: urn:nbn:se:mau:diva-55888DOI: 10.3390/molecules27217628ISI: 000883556400001PubMedID: 36364455Scopus ID: 2-s2.0-85141759223OAI: oai:DiVA.org:mau-55888DiVA, id: diva2:1709980
Funder
Swedish Research Council, 2018-03990Swedish Research Council, 2018-04833Available from: 2022-11-10 Created: 2022-11-10 Last updated: 2024-06-17Bibliographically approved

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Del Giudice, RitaParacini, NicolòRoosen-Runge, FelixCárdenas, Marité

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Del Giudice, RitaParacini, NicolòLaursen, TomasRoosen-Runge, FelixCárdenas, Marité
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